Bacterial motility: machinery and mechanisms

Nature Reviews Microbiology, Год журнала: 2021, Номер 20(3), С. 161 - 173

Опубликована: Сен. 21, 2021

Язык: Английский

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Campylobacter jejuni in Poultry: Pathogenesis and Control Strategies

Microorganisms, Год журнала: 2022, Номер 10(11), С. 2134 - 2134

Опубликована: Окт. 28, 2022

C. jejuni is the leading cause of human foodborne illness associated with poultry, beef, and pork consumption. highly prevalent in commercial poultry farms, where horizontal transmission from environment considered to be primary source jejuni. As an enteric pathogen, expresses virulence factors regulated by a two-component system that mediates jejuni's ability survive host. survives reproduces avian intestinal mucus. The mucus sulfated sialylated compared modulating pathogenicity into near commensal bacteria poultry. Birds are usually infected two four weeks age remain colonized until they reach market age. A small dose (around 35 CFU/mL) sufficient for successful bird colonization. In U.S., chickens raised under antibiotic-free environments, additional strategies required reduce prevalence on broilers farms. Strict biosecurity measures can decrease more than 50% at Vaccination probiotics, prebiotics, synbiotics, organic acids, bacteriophages, bacteriocins, quorum sensing inhibitors supplementation improve gut health competitively exclude load broilers. Most mentioned showed promising results; however, not fully implemented production. Current knowledge morphology, transmission, pathogenesis available preharvest colonization addressed this review.

Язык: Английский

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Wrapped Up: The Motility of Polarly Flagellated Bacteria

Annual Review of Microbiology, Год журнала: 2022, Номер 76(1), С. 349 - 367

Опубликована: Июнь 2, 2022

A huge number of bacterial species are motile by flagella, which allow them to actively move toward favorable environments and away from hazardous areas conquer new habitats. The general perception flagellum-mediated movement chemotaxis is dominated the Escherichia coli paradigm, with its peritrichous flagellation famous run-and-tumble navigation pattern, has shaped view on how bacteria swim navigate in chemical gradients. However, a significant amount-more likely majority-of exhibit (bi)polar flagellar localization pattern instead lateral flagella. Accordingly, these have evolved very different mechanisms for chemotaxis. Here, we review earlier recent findings various modes motility mediated polar

Язык: Английский

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Campylobacter jejuni: targeting host cells, adhesion, invasion, and survival

Applied Microbiology and Biotechnology, Год журнала: 2023, Номер 107(9), С. 2725 - 2754

Опубликована: Март 21, 2023

Campylobacter jejuni, causing strong enteritis, is an unusual bacterium with numerous peculiarities. Chemotactically controlled motility in viscous milieu allows targeted navigation to intestinal mucus and colonization. By phase variation, quorum sensing, extensive O-and N-glycosylation use of the flagellum as type-3-secretion system C. jejuni adapts effectively environmental conditions. utilizes proteases open cell-cell junctions subsequently transmigrates paracellularly. Fibronectin at basolateral side polarized epithelial cells serves binding site for adhesins CadF FlpA, leading intracellular signaling, which again triggers membrane ruffling reduced host cell migration by focal adhesion. Cell contacts results its secretion invasion antigens, induce paxillin-independent pathway. In addition fibronectin-binding proteins, other target structures lectins their corresponding sugar are involved host-pathogen interaction. Invasion into depends on structures. Fibronectin, clathrin, dynein influence cytoskeletal restructuring, endocytosis, vesicular transport, through different mechanisms. can persist over a 72-h period cell. Campylobacter-containing vacuoles, avoid fusion lysosomes enter perinuclear space via dynein, inducing signaling pathways. Secretion cytolethal distending toxin directs programmed death, including pyroptotic release proinflammatory substances from destroyed compartments. The immune reacts inflammatory cascade participation cells. development autoantibodies, directed not only against lipooligosaccharides, but also endogenous gangliosides, autoimmune diseases. Lesions epithelium result loss electrolytes, water, blood, diarrhea, flushes out containing jejuni. Together response system, this limits infection time. Based structural interactions between bacterium, virulence mechanisms, effects that characterize process wide variety targets attenuation pathogen be characterized. review summarizes strategies interaction should stimulate innovative research towards improved definition future drug development. KEY POINTS: • Bacterial adhesion strictly coordinated processes, serve prevent infection. Reaction signalling depend type. factors used antivirulence compounds.

Язык: Английский

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The Missing Pieces: The Role of Secretion Systems in Campylobacter jejuni Virulence

Biomolecules, Год журнала: 2023, Номер 13(1), С. 135 - 135

Опубликована: Янв. 9, 2023

Campylobacter jejuni is likely the most common bacterial cause of gastroenteritis worldwide, responsible for millions cases inflammatory diarrhea characterized by severe abdominal cramps and blood in stool. Further, C. infections are associated with post-infection sequelae developed countries malnutrition growth-stunting low- middle-income countries. Despite increasing prevalence disease, campylobacteriosis, recognition that this pathogen a serious health threat, our understanding pathogenesis remains incomplete. In review, we focus on secretion systems proposed to contribute host-cell interactions survival host. Moreover, have applied genomics approach defining structural mechanistic features type III, IV, VI systems. Special attention focused flagellar III system prediction putative effectors, given proteins exported via essential host cell invasion response. We conclude does not possess IV relies establish niche potentiate disease.

Язык: Английский

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More than propellers: how flagella shape bacterial motility behaviors

Current Opinion in Microbiology, Год журнала: 2021, Номер 61, С. 73 - 81

Опубликована: Апрель 9, 2021

Bacteria use a wide variety of flagellar architectures to navigate their environment. While the iconic run-tumble motility strategy peritrichously flagellated Escherichia coli has been well studied, recent work revealed new behaviors that can be achieved with different architectures, such as single, bundled, or opposing polar flagella. The discovery various gymnastics flicking and wrapping is increasingly shifting view from flagella passive propellers versatile appendages used in range conformations. Here, we review observations how shape summarize nascent structure-function map linking flagellation behavior.

Язык: Английский

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Campylobacter Virulence Factors and Molecular Host–Pathogen Interactions

Current topics in microbiology and immunology, Год журнала: 2021, Номер unknown, С. 169 - 202

Опубликована: Янв. 1, 2021

Язык: Английский

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Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Март 17, 2022

Abstract Flagellar filaments function as the propellers of bacterial flagellum and their supercoiling is key to motility. The outer domains on surface filament are non-critical for motility in many bacteria structures functions not conserved. Here, we show atomic cryo-electron microscopy flagellar from enterohemorrhagic Escherichia coli O157:H7, enteropathogenic E. O127:H6, Achromobacter , Sinorhizobium meliloti where dimerize or tetramerize form either a sheath screw-like surface. These dimers formed by 180° rotations half domains. domain (ODS) plays role stabilizing an intermediate waveform prolonging tumbling cells. Bacteria with these ODS commonly found soil human intestinal environments relatively high viscosity suggesting dimerization environments.

Язык: Английский

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An overview of the structure and function of the flagellar hook FlgE protein

World Journal of Microbiology and Biotechnology, Год журнала: 2023, Номер 39(5)

Опубликована: Март 21, 2023

Язык: Английский

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A hybrid receptor binding protein enables phage F341 infection of Campylobacter by binding to flagella and lipooligosaccharides

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Фев. 1, 2024

Flagellotropic bacteriophages are interesting candidates as therapeutics against pathogenic bacteria dependent on flagellar motility for colonization and causing disease. Yet, phage resistance other than loss of has been scarcely studied. Here we developed a soft agar assay to study flagellotropic F341 in motile Campylobacter jejuni . We found that adsorption was prevented by diverse genetic mutations the lipooligosaccharides forming secondary receptor F341. Genome sequencing showed belongs Fletchervirus genus otherwise comprising capsular-dependent C. phages. Interestingly, encodes hybrid binding protein (RBP) predicted short tail fiber showing partial similarity RBP1 encoded , but with domain similar H CJIE1 prophages. Thus, prophages may represent pool from where lytic phages can acquire new traits like recognition receptors.

Язык: Английский

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