High glucose induces renal tubular epithelial cell senescence by inhibiting autophagic flux

Human Cell, Год журнала: 2025, Номер 38(2)

Опубликована: Янв. 10, 2025

Язык: Английский

---

Mechanisms of female reproductive toxicity in pigs induced by exposure to environmental pollutants

Molecules and Cells, Год журнала: 2024, Номер 47(5), С. 100065 - 100065

Опубликована: Апрель 26, 2024

Environmental pollutants, including endocrine disruptors, heavy metals, nanomaterials, and pesticides, have been detected in various ecosystems are of growing global concern. The potential for toxicity to non-target organisms has consistently raised is being studied using animal models. In this review, we focus on pesticides frequently the environment investigate their exposure livestock. Owing reproductive similarities between humans pigs, vitro porcine models such as oocytes, trophectoderm cells, luminal epithelial used verify toxicity. These cell lines study toxic mechanisms induced by environmental toxicants, organophosphate insecticides, pyrethroid dinitroaniline herbicides, diphenyl ether which persist threaten livestock health. Collectively, these results indicate that can induce female pigs suggest possibility adverse effects other species. also possible humans, requires further investigation.

Язык: Английский

---

Brief guide to senescence assays using cultured mammalian cells

Molecules and Cells, Год журнала: 2024, Номер 47(9), С. 100102 - 100102

Опубликована: Июль 23, 2024

Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present brief guide to mammalian assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, expression secretory phenotype (SASP). This work will be useful for biologists minimum expertise assays.

Язык: Английский

---

Propanil impairs organ development in zebrafish by inducing apoptosis and inhibiting mitochondrial respiration

Journal of Hazardous Materials, Год журнала: 2024, Номер 480, С. 136364 - 136364

Опубликована: Окт. 31, 2024

Язык: Английский

---

Autophagy regulation and redox perturbation by transcrocetin suppress the growth of endometriosis

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 173, С. 116284 - 116284

Опубликована: Фев. 23, 2024

Until non-hormonal therapeutic targets for endometriosis are suggested, we focused on mitochondrial function and autophagy regulation in the disease. Transcrocetin is a carotenoid retinoic acid with high antioxidant potency antiproliferative effects several diseases. In this study, demonstrated mechanisms of transcrocetin using End1/E6E7 VK2/E6E7 cell lines. suppressed viability proliferation these lines did not affect normal uterine stromal cells. p21 Waf1/Cip1 as cycle regulator target p53, were increased by caused G1 arrest via inhibition cyclin-dependent kinase activity, which might further cause death. Furthermore, confirmed endoplasmic reticulum stress calcium ion dysregulation cytosol matrix, disrupting membrane potential. Mitochondrial bioenergetics transcrocetin, oxidative phosphorylation-related gene expression was downregulated. Moreover, cells regulated transcrocetin-induced stress. Finally, verified impairment autophagic flux following pre-treatment chloroquine. Therefore, may be potent alternative endometriosis.

Язык: Английский

---

Heat-killed probiotic Levilactobacillus brevis MKAK9 and its exopolysaccharide promote longevity by modulating aging hallmarks and enhancing immune responses in Caenorhabditis elegans

Immunity & Ageing, Год журнала: 2024, Номер 21(1)

Опубликована: Авг. 2, 2024

Proteostasis is a critical aging hallmark responsible for removing damaged or misfolded proteins and their aggregates by improving proteasomal degradation through the autophagy-lysosome pathway (ALP) ubiquitin-proteasome system (UPS). Research on impact of heat-killed probiotic bacteria structural components hallmarks innate immune responses scarce, yet enhancing these effects could potentially delay age-related diseases.

Язык: Английский

---

The Emerging Roles of Vacuolar-Type ATPase-Dependent Lysosomal Acidification in Cardiovascular Disease

Biomolecules, Год журнала: 2025, Номер 15(4), С. 525 - 525

Опубликована: Апрель 3, 2025

The vacuolar-type ATPase (V-ATPase) is a multi-subunit enzyme complex that maintains lysosomal acidification, critical process for cellular homeostasis. By controlling the pH within lysosomes, V-ATPase contributes to overall homeostasis, helping maintain balance between degradation and synthesis of components. Dysfunction impairs leading accumulation undigested materials contributing various diseases, including cardiovascular diseases (CVDs) like atherosclerosis myocardial disease. Furthermore, V-ATPase's role in function suggests potential therapeutic strategies targeting this mitigate disease progression. Understanding mechanisms by which influences pathology essential developing novel treatments aimed at improving outcomes patients with heart vascular diseases.

Язык: Английский

---

Developmental and Organ Toxicity of Fenpropimorph in Zebrafish: Involvement of Apoptosis and Inflammation

Chemico-Biological Interactions, Год журнала: 2025, Номер unknown, С. 111512 - 111512

Опубликована: Апрель 1, 2025

Язык: Английский

---

Impacts of a high-glucose diet or starvation on microRNA-transcription factor networks in Caenorhabditis elegans through Boolean mathematical modeling

Biosystems, Год журнала: 2025, Номер unknown, С. 105503 - 105503

Опубликована: Май 1, 2025

Язык: Английский

---

Brief guide to Caenorhabditis elegans survival assays

Molecules and Cells, Год журнала: 2025, Номер unknown, С. 100232 - 100232

Опубликована: Май 1, 2025

Язык: Английский

---