Wings of Change: aPKC/FoxP-dependent plasticity in steering motor neurons underlies operant selflearning inDrosophila DOI Creative Commons

Andreas Ehweiner,

Carsten Duch, Björn Brembs

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Дек. 16, 2022

Abstract Background Motor learning is central to human existence, such as speak or walk, sports moves, rehabilitation after injury. Evidence suggests that all forms of motor share an evolutionarily conserved molecular plasticity pathway. Here, we present novel insights into the neural processes underlying operant self-learning, a form in fruit fly Drosophila . Methods We operantly trained wild type and transgenic flies, tethered at torque meter, task required them initiate maintain turning maneuvers around their vertical body axis (yaw torque). combined this behavioral experiment with peptide expression, CRISPR/Cas9-mediated, spatio-temporally controlled gene knock-out confocal microscopy. Results find expression atypical protein kinase C (aPKC) direct wing steering motoneurons co-expressing transcription factor FoxP necessary for aPKC likely acts via non-canonical pathways. also found it takes more than week CRISPR/Cas9-mediated knockout adult animals impair learning, suggesting self-learning. Conclusions Our experiments suggest that, , co-expression motoneurons. Some these neurons control beat amplitude when generating optomotor responses, have discovered modulation behavior pathways flies.

Язык: Английский

Very-long-chain fatty acids are crucial to neuronal polarity by providing sphingolipids to lipid rafts DOI Creative Commons
Atsuko Honda, Motohiro Nozumi, Yasuyuki Ito

и другие.

Cell Reports, Год журнала: 2023, Номер 42(10), С. 113195 - 113195

Опубликована: Окт. 1, 2023

Fatty acids have long been considered essential to brain development; however, the involvement of their synthesis in nervous system formation is unclear. We generate mice with knockout GPSN2, an enzyme for very-long-chain fatty (VLCFAs) and investigate effects. Both GPSN2−/− GPSN2+/− show abnormal neuronal networks as a result impaired polarity determination. Lipidomics embryos reveal that ceramide specifically inhibited depending on FA length; namely, VLCFA-containing reduced. demonstrate lipid rafts are highly enriched growth cones neurons lose gangliosides membranes. Application C24:0 ceramide, but not C16:0 or phosphatidylcholine, rescues both determination lipid-raft density cone. Taken together, our results indicate VLCFA contributes physiological development network formation, particular through rafts.

Язык: Английский

Процитировано

18
Protein Kinase C (PKC) in Neurological Health: Implications for Alzheimer’s Disease and Chronic Alcohol Consumption DOI Creative Commons
R.S. Kaler, Shouvik Kumar Nandy, Anupam Jyoti

и другие.

Brain Sciences, Год журнала: 2024, Номер 14(6), С. 554 - 554

Опубликована: Май 29, 2024

Protein kinase C (PKC) is a diverse enzyme family crucial for cell signalling in various organs. Its dysregulation linked to numerous diseases, including cancer, cardiovascular disorders, and neurological problems. In the brain, PKC plays pivotal roles synaptic plasticity, learning, memory, neuronal survival. Specifically, PKC’s involvement Alzheimer’s Disease (AD) pathogenesis of significant interest. The has been AD. This review elucidates role health, particularly its implications AD chronic alcohol addiction. AD, characterised by neurodegeneration, implicates dysfunction cognitive decline. Conversely, consumption elicits neural adaptations intertwined with signalling, exacerbating addictive behaviours. By unravelling these afflictions, potential therapeutic avenues emerge, offering promise ameliorating their debilitating effects. navigates complex interplay between PKC, pathology, addiction, illuminating pathways future neurotherapeutic interventions.

Язык: Английский

Процитировано

7
Blastocyst Cavity Expansion Promotes Cell Polarization During Early Development of Mouse Embryos DOI
Zheng Guo,

Xinxin Lv,

Jianwen Li

и другие.

Birth Defects Research, Год журнала: 2025, Номер 117(5)

Опубликована: Май 1, 2025

ABSTRACT Background Cell polarization is an important morphological process that crucial for the formation and function of tissues organs. The blastocyst cavity expansion apparent event during second cell fate specification in mouse embryos, yet its impact on remains unclear. In this study, we investigate effects polarization. Methods methods study involve hyperosmotic treatment or disruption TE cortical tension by laser ablation, combined with immunofluorescence. Results We found inhibition through hypertonic ablation suppresses levels ζ isotype protein kinase C (PKC ζ) which a member atypical PKC subfamily involved further embryonic stages E3.5 to E4.0, expression extracellular signal‐regulated 1 (ERK1), key upstream regulator ζ, altered similar tendency indicating potential regulatory ERK1 early development embryos. Conclusions This reveals mechanical behavior embryos mammalian relationship between provides new understanding, thereby offering fresh insights screening detection indicators normal development.

Язык: Английский

Процитировано

0
Stepwise modulation of apical orientational cell adhesions for vertebrate neurulation DOI Open Access
Lili Zhang, Xiangyun Wei

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Год журнала: 2023, Номер 98(6), С. 2271 - 2283

Опубликована: Авг. 3, 2023

Neurulation transforms the neuroectoderm into neural tube. This transformation relies on reorganising configurational relationships between orientations of intrinsic polarities neighbouring cells. These orientational intercellular are established, maintained, and modulated by cell adhesions (OCAs). Here, using zebrafish (Danio rerio) neurulation as a major model, we propose new perspective how OCAs contribute to parallel, antiparallel, opposing that underlie plate-keel-rod-tube transformation, stepwise process aggregation followed cord hollowing. We also discuss in may be regulated various adhesion molecules, including cadherins, Eph/Ephrins, Claudins, Occludins, Crumbs, Na+ /K+ -ATPase, integrins. By comparing among species, reveal antiparallel represent conserved mechanism for fusion Throughout, highlight some outstanding questions regarding neurulation. Answers these will help us understand better mechanisms tubulogenesis many tissues.

Язык: Английский

Процитировано

4
Wings of Change: aPKC/FoxP-dependent plasticity in steering motor neurons underlies operant self-learning in Drosophila DOI Creative Commons

Andreas Ehweiner,

Carsten Duch, Björn Brembs

и другие.

F1000Research, Год журнала: 2024, Номер 13, С. 116 - 116

Опубликована: Фев. 19, 2024

Background Motor learning is central to human existence, such as speak or walk, sports moves, rehabilitation after injury. Evidence suggests that all forms of motor share an evolutionarily conserved molecular plasticity pathway. Here, we present novel insights into the neural processes underlying operant self-learning, a form in fruit fly Drosophila. Methods We operantly trained wild type and transgenic Drosophila flies, tethered at torque meter, task required them initiate maintain turning maneuvers around their vertical body axis (yaw torque). combined this behavioral experiment with peptide expression, CRISPR/Cas9-mediated, spatio-temporally controlled gene knock-out confocal microscopy. Results find expression atypical protein kinase C (aPKC) direct wing steering motoneurons co-expressing transcription factor FoxP necessary for aPKC likely acts via non-canonical pathways. also found it takes more than week CRISPR/Cas9-mediated knockout adult animals impair learning, suggesting self-learning. Conclusions Our experiments suggest that, Drosophila, co-expression motoneurons. Some these neurons control beat amplitude when generating optomotor responses, have discovered modulation behavior pathways FoxP flies.

Язык: Английский

Процитировано

1
Entosis implicates a new role for P53 in microcephaly pathogenesis, beyond apoptosis DOI
Noelle A. Sterling, Seo‐Hee Cho, Seonhee Kim

и другие.

BioEssays, Год журнала: 2024, Номер 46(8)

Опубликована: Май 22, 2024

Abstract Entosis, a form of cell cannibalism, is newly discovered pathogenic mechanism leading to the development small brains, termed microcephaly, in which P53 activation was found play major role. Microcephaly with entosis, Pals1 mutant mice, displays that promotes entosis and apoptotic death. This previously unappreciated represents novel cellular dynamic dividing cortical progenitors responsible for loss. To date, various recent models microcephaly have bolstered importance death microcephaly. caused by mitotic delay or DNA damage manifests can be suppressed removal these animal models. Such genetic studies attest as quality control meant eliminate genomically unfit cells minimal involvement actual function associated genes. In this review, we summarize known role variety introduce wherein entotic cannibalism neural triggered activation.

Язык: Английский

Процитировано

1
Wings of Change: aPKC/FoxP-dependent plasticity in steering motor neurons underlies operant self-learning in Drosophila DOI Creative Commons

Andreas Ehweiner,

Carsten Duch, Björn Brembs

и другие.

F1000Research, Год журнала: 2024, Номер 13, С. 116 - 116

Опубликована: Июнь 11, 2024

Background Motor learning is central to human existence, such as speak or walk, sports moves, rehabilitation after injury. Evidence suggests that all forms of motor share an evolutionarily conserved molecular plasticity pathway. Here, we present novel insights into the neural processes underlying operant self-learning, a form in fruit fly Drosophila. Methods We operantly trained wild type and transgenic Drosophila flies, tethered at torque meter, task required them initiate maintain turning maneuvers around their vertical body axis (yaw torque). combined this behavioral experiment with peptide expression, CRISPR/Cas9-mediated, spatio-temporally controlled gene knock-out confocal microscopy. Results find expression atypical protein kinase C (aPKC) direct wing steering motoneurons co-expressing transcription factor FoxP necessary for aPKC likely acts via non-canonical pathways. also found it takes more than week CRISPR/Cas9-mediated knockout adult animals impair learning, suggesting self-learning. Conclusions Our experiments suggest that, Drosophila, co-expression motoneurons. Some these neurons control beat amplitude when generating optomotor responses, have discovered modulation behavior pathways FoxP flies.

Язык: Английский

Процитировано

1
Mechanistic investigation of the differential synergistic neurotoxicity between pesticide metam sodium and copper or zinc DOI
Chen Shen,

Zhi-Guo Sheng,

Jie Shao

и другие.

Chemosphere, Год журнала: 2023, Номер 328, С. 138430 - 138430

Опубликована: Март 22, 2023

Язык: Английский

Процитировано

3
Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma DOI
Suzannah Miller,

Maria Hoh,

Christopher C. Ebmeier

и другие.

Molecular Biology of the Cell, Год журнала: 2023, Номер 35(3)

Опубликована: Дек. 20, 2023

The WRAMP structure is a protein network associated with tail-end actomyosin contractility, membrane retraction, and directional persistence during cell migration. A marker of structures melanoma adhesion molecule (MCAM) which dynamically polarizes to the rear. However, factors that mediate MCAM polarization are still unknown. In this study, BioID using as bait identifies ERM family proteins, moesin, ezrin, radixin, components. We also present novel image analysis pipeline, Protein Polarity by Percentile ("3P"), classifies machine learning facilitates quantitative analysis. Using 3P, we find depletion lesser extent decreases proportion cells polarized MCAM. Furthermore, although copolarized proteins show high spatial overlap, 3P subpopulations closer periphery. Live-cell imaging confirms tightly coordinated, but enrich at edge first. Finally, deletion juxtamembrane segment in previously shown promote interactions impedes polarization. Our findings highlight requirement for recruitment an advanced computational tool characterize

Язык: Английский

Процитировано

1
Time-resolved function of cell polarity kinases PRKCZ and PRKCI in CNS myelination DOI Open Access
María E. Mercau,

Rieke-Marie Hackbarth,

Xinran Liu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 23, 2024

ABSTRACT The atypical Protein Kinase C (aPKC) is a critical component of the PAR polarity complex, as well cell in general. There are two paralogs aPKC mammalian genome. Their expression pattern and functional role oligodendrocyte lineage differentiation development remains heretofore uncharacterized. Here we show that both – PRKCI, primary transcript Prkci PKMz, an alternate Prkcz expressed during differentiation, yet functionally non-redundant. Asymmetric division early specification fate stage required paralogs. By contrast, maturation involving membrane polarization myelination axons continued to require but no longer needed PRKCI. Taken together, our results indicate two-stage aPKCs development.

Язык: Английский

Процитировано

0