Wings of Change: aPKC/FoxP-dependent plasticity in steering motor neurons underlies operant selflearning inDrosophila DOI Creative Commons

Andreas Ehweiner,

Carsten Duch, Björn Brembs

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 16, 2022

Abstract Background Motor learning is central to human existence, such as speak or walk, sports moves, rehabilitation after injury. Evidence suggests that all forms of motor share an evolutionarily conserved molecular plasticity pathway. Here, we present novel insights into the neural processes underlying operant self-learning, a form in fruit fly Drosophila . Methods We operantly trained wild type and transgenic flies, tethered at torque meter, task required them initiate maintain turning maneuvers around their vertical body axis (yaw torque). combined this behavioral experiment with peptide expression, CRISPR/Cas9-mediated, spatio-temporally controlled gene knock-out confocal microscopy. Results find expression atypical protein kinase C (aPKC) direct wing steering motoneurons co-expressing transcription factor FoxP necessary for aPKC likely acts via non-canonical pathways. also found it takes more than week CRISPR/Cas9-mediated knockout adult animals impair learning, suggesting self-learning. Conclusions Our experiments suggest that, , co-expression motoneurons. Some these neurons control beat amplitude when generating optomotor responses, have discovered modulation behavior pathways flies.

Language: Английский

Protein Kinase C (PKC) in Neurological Health: Implications for Alzheimer’s Disease and Chronic Alcohol Consumption DOI Creative Commons
R.S. Kaler, Shouvik Kumar Nandy, Anupam Jyoti

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(6), P. 554 - 554

Published: May 29, 2024

Protein kinase C (PKC) is a diverse enzyme family crucial for cell signalling in various organs. Its dysregulation linked to numerous diseases, including cancer, cardiovascular disorders, and neurological problems. In the brain, PKC plays pivotal roles synaptic plasticity, learning, memory, neuronal survival. Specifically, PKC’s involvement Alzheimer’s Disease (AD) pathogenesis of significant interest. The has been AD. This review elucidates role health, particularly its implications AD chronic alcohol addiction. AD, characterised by neurodegeneration, implicates dysfunction cognitive decline. Conversely, consumption elicits neural adaptations intertwined with signalling, exacerbating addictive behaviours. By unravelling these afflictions, potential therapeutic avenues emerge, offering promise ameliorating their debilitating effects. navigates complex interplay between PKC, pathology, addiction, illuminating pathways future neurotherapeutic interventions.

Language: Английский

Citations

7
Very-long-chain fatty acids are crucial to neuronal polarity by providing sphingolipids to lipid rafts DOI Creative Commons
Atsuko Honda, Motohiro Nozumi, Yasuyuki Ito

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(10), P. 113195 - 113195

Published: Oct. 1, 2023

Fatty acids have long been considered essential to brain development; however, the involvement of their synthesis in nervous system formation is unclear. We generate mice with knockout GPSN2, an enzyme for very-long-chain fatty (VLCFAs) and investigate effects. Both GPSN2−/− GPSN2+/− show abnormal neuronal networks as a result impaired polarity determination. Lipidomics embryos reveal that ceramide specifically inhibited depending on FA length; namely, VLCFA-containing reduced. demonstrate lipid rafts are highly enriched growth cones neurons lose gangliosides membranes. Application C24:0 ceramide, but not C16:0 or phosphatidylcholine, rescues both determination lipid-raft density cone. Taken together, our results indicate VLCFA contributes physiological development network formation, particular through rafts.

Language: Английский

Citations

15
Blastocyst Cavity Expansion Promotes Cell Polarization During Early Development of Mouse Embryos DOI
Zheng Guo,

Xinxin Lv,

Jianwen Li

et al.

Birth Defects Research, Journal Year: 2025, Volume and Issue: 117(5)

Published: May 1, 2025

ABSTRACT Background Cell polarization is an important morphological process that crucial for the formation and function of tissues organs. The blastocyst cavity expansion apparent event during second cell fate specification in mouse embryos, yet its impact on remains unclear. In this study, we investigate effects polarization. Methods methods study involve hyperosmotic treatment or disruption TE cortical tension by laser ablation, combined with immunofluorescence. Results We found inhibition through hypertonic ablation suppresses levels ζ isotype protein kinase C (PKC ζ) which a member atypical PKC subfamily involved further embryonic stages E3.5 to E4.0, expression extracellular signal‐regulated 1 (ERK1), key upstream regulator ζ, altered similar tendency indicating potential regulatory ERK1 early development embryos. Conclusions This reveals mechanical behavior embryos mammalian relationship between provides new understanding, thereby offering fresh insights screening detection indicators normal development.

Language: Английский

Citations

0
Stepwise modulation of apical orientational cell adhesions for vertebrate neurulation DOI Open Access
Lili Zhang, Xiangyun Wei

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2023, Volume and Issue: 98(6), P. 2271 - 2283

Published: Aug. 3, 2023

Neurulation transforms the neuroectoderm into neural tube. This transformation relies on reorganising configurational relationships between orientations of intrinsic polarities neighbouring cells. These orientational intercellular are established, maintained, and modulated by cell adhesions (OCAs). Here, using zebrafish (Danio rerio) neurulation as a major model, we propose new perspective how OCAs contribute to parallel, antiparallel, opposing that underlie plate-keel-rod-tube transformation, stepwise process aggregation followed cord hollowing. We also discuss in may be regulated various adhesion molecules, including cadherins, Eph/Ephrins, Claudins, Occludins, Crumbs, Na+ /K+ -ATPase, integrins. By comparing among species, reveal antiparallel represent conserved mechanism for fusion Throughout, highlight some outstanding questions regarding neurulation. Answers these will help us understand better mechanisms tubulogenesis many tissues.

Language: Английский

Citations

4
Wings of Change: aPKC/FoxP-dependent plasticity in steering motor neurons underlies operant self-learning in Drosophila DOI Creative Commons

Andreas Ehweiner,

Carsten Duch, Björn Brembs

et al.

F1000Research, Journal Year: 2024, Volume and Issue: 13, P. 116 - 116

Published: June 11, 2024

Background Motor learning is central to human existence, such as speak or walk, sports moves, rehabilitation after injury. Evidence suggests that all forms of motor share an evolutionarily conserved molecular plasticity pathway. Here, we present novel insights into the neural processes underlying operant self-learning, a form in fruit fly Drosophila. Methods We operantly trained wild type and transgenic Drosophila flies, tethered at torque meter, task required them initiate maintain turning maneuvers around their vertical body axis (yaw torque). combined this behavioral experiment with peptide expression, CRISPR/Cas9-mediated, spatio-temporally controlled gene knock-out confocal microscopy. Results find expression atypical protein kinase C (aPKC) direct wing steering motoneurons co-expressing transcription factor FoxP necessary for aPKC likely acts via non-canonical pathways. also found it takes more than week CRISPR/Cas9-mediated knockout adult animals impair learning, suggesting self-learning. Conclusions Our experiments suggest that, Drosophila, co-expression motoneurons. Some these neurons control beat amplitude when generating optomotor responses, have discovered modulation behavior pathways FoxP flies.

Language: Английский

Citations

1
Wings of Change: aPKC/FoxP-dependent plasticity in steering motor neurons underlies operant self-learning in Drosophila DOI Creative Commons

Andreas Ehweiner,

Carsten Duch, Björn Brembs

et al.

F1000Research, Journal Year: 2024, Volume and Issue: 13, P. 116 - 116

Published: Feb. 19, 2024

Background Motor learning is central to human existence, such as speak or walk, sports moves, rehabilitation after injury. Evidence suggests that all forms of motor share an evolutionarily conserved molecular plasticity pathway. Here, we present novel insights into the neural processes underlying operant self-learning, a form in fruit fly Drosophila. Methods We operantly trained wild type and transgenic Drosophila flies, tethered at torque meter, task required them initiate maintain turning maneuvers around their vertical body axis (yaw torque). combined this behavioral experiment with peptide expression, CRISPR/Cas9-mediated, spatio-temporally controlled gene knock-out confocal microscopy. Results find expression atypical protein kinase C (aPKC) direct wing steering motoneurons co-expressing transcription factor FoxP necessary for aPKC likely acts via non-canonical pathways. also found it takes more than week CRISPR/Cas9-mediated knockout adult animals impair learning, suggesting self-learning. Conclusions Our experiments suggest that, Drosophila, co-expression motoneurons. Some these neurons control beat amplitude when generating optomotor responses, have discovered modulation behavior pathways FoxP flies.

Language: Английский

Citations

1
Entosis implicates a new role for P53 in microcephaly pathogenesis, beyond apoptosis DOI
Noelle A. Sterling, Seo‐Hee Cho, Seonhee Kim

et al.

BioEssays, Journal Year: 2024, Volume and Issue: 46(8)

Published: May 22, 2024

Abstract Entosis, a form of cell cannibalism, is newly discovered pathogenic mechanism leading to the development small brains, termed microcephaly, in which P53 activation was found play major role. Microcephaly with entosis, Pals1 mutant mice, displays that promotes entosis and apoptotic death. This previously unappreciated represents novel cellular dynamic dividing cortical progenitors responsible for loss. To date, various recent models microcephaly have bolstered importance death microcephaly. caused by mitotic delay or DNA damage manifests can be suppressed removal these animal models. Such genetic studies attest as quality control meant eliminate genomically unfit cells minimal involvement actual function associated genes. In this review, we summarize known role variety introduce wherein entotic cannibalism neural triggered activation.

Language: Английский

Citations

1
Mechanistic investigation of the differential synergistic neurotoxicity between pesticide metam sodium and copper or zinc DOI
Chen Shen,

Zhi-Guo Sheng,

Jie Shao

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 328, P. 138430 - 138430

Published: March 22, 2023

Language: Английский

Citations

3
Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma DOI
Suzannah Miller,

Maria Hoh,

Christopher C. Ebmeier

et al.

Molecular Biology of the Cell, Journal Year: 2023, Volume and Issue: 35(3)

Published: Dec. 20, 2023

The WRAMP structure is a protein network associated with tail-end actomyosin contractility, membrane retraction, and directional persistence during cell migration. A marker of structures melanoma adhesion molecule (MCAM) which dynamically polarizes to the rear. However, factors that mediate MCAM polarization are still unknown. In this study, BioID using as bait identifies ERM family proteins, moesin, ezrin, radixin, components. We also present novel image analysis pipeline, Protein Polarity by Percentile ("3P"), classifies machine learning facilitates quantitative analysis. Using 3P, we find depletion lesser extent decreases proportion cells polarized MCAM. Furthermore, although copolarized proteins show high spatial overlap, 3P subpopulations closer periphery. Live-cell imaging confirms tightly coordinated, but enrich at edge first. Finally, deletion juxtamembrane segment in previously shown promote interactions impedes polarization. Our findings highlight requirement for recruitment an advanced computational tool characterize

Language: Английский

Citations

1
The Lego hypothesis of tissue morphogenesis: stereotypic organization of parallel orientational cell adhesions for epithelial self‐assembly DOI Creative Commons
Lili Zhang, Xiangyun Wei

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

How tissues develop distinct structures remains poorly understood. We propose herein the Lego hypothesis of tissue morphogenesis, which states that during topographical properties cell surface adhesion molecules can be dynamically altered and polarised by regulating spatiotemporal expression localization orientational (OCA) cell-autonomously non-cell-autonomously, thus modulating cells into unique pieces for self-assembling cytoarchitectures. This concept exemplified epithelial in are coalesced a sheet many types adhesions. Among them, parallel OCAs (pOCAs) at lateral membranes essential configuring parallel. Major pOCAs include Na

Language: Английский

Citations

0