bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 19, 2024
Highlights
Alveolar
organoids
are
formed
with
a
two-step,
Matrigel-free
method
in
semi-synthetic
hyaluronic
acid
(HA)
hydrogel
The
two-step
offers
control
over
alveolar
size,
density,
and
growth
maintain
their
AT2
identity
HA
hydrogels
secrete
nascent
extracellular
matrix
supporting
organoid
without
Matrigel
Summary
Human
induced
pluripotent
stem
cell
(iPSC)
derived
have
emerged
as
system
to
model
the
epithelium
homeostasis
disease.
However,
typically
grown
Matrigel,
mouse-sarcoma
basement
membrane
that
poor
properties,
prompting
development
of
synthetic
alternative.
Here,
we
develop
culture
involves
pre-aggregation
hydrogel-based
microwells
followed
by
embedding
supports
growth,
while
also
offering
considerable
properties.
We
find
aggregated
own
(ECM)
both
upon
hydrogels.
Thus,
gels
described
here
allow
us
de-couple
exogenous
ECM
order
interrogate
role
formation.
Trends in Cell Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Brain
organoids
are
important
3D
models
for
studying
human
brain
development,
disease,
and
evolution.
To
overcome
some
of
the
existing
limitations
that
affect
organoid
quality,
reproducibility,
characteristics,
in
vivo
resemblance,
current
efforts
directed
to
improve
their
physiological
relevance
by
exploring
different,
yet
interconnected,
routes.
In
this
review,
these
approaches
latest
developments
discussed,
including
stem
cell
optimization,
refining
morphogen
administration
strategies,
altering
extracellular
matrix
(ECM)
niche,
manipulating
tissue
architecture
mimic
morphogenesis.
Additionally,
strategies
increase
diversity
enhance
maturation,
such
as
establishing
co-cultures,
assembloids,
xenotransplantation,
reviewed.
We
explore
how
various
factors
can
be
tuned
intermingled
speculate
on
future
avenues
towards
even
more
physiologically-advanced
organoids.
Materials Today Bio,
Год журнала:
2025,
Номер
unknown, С. 101561 - 101561
Опубликована: Фев. 1, 2025
Spinal
cord
organoids
(SCOs)
are
in
vitro
models
that
faithfully
recapitulate
the
basic
tissue
architecture
and
cell
types
of
spinal
play
a
crucial
role
developmental
studies,
disease
modeling,
drug
screening.
Physiological
cues
required
for
proliferation
differentiation
during
SCO
culture.
However,
commonly
used
basement
membrane
matrix
products,
such
as
Matrigel®,
lack
tissue-specific
biophysical
signals.
The
current
study
utilizes
decellularization
process
to
fabricate
tissue-derived
hydrogel
from
porcine
retain
intrinsic
components.
This
gel
system
supported
an
expanded
neuroepithelial
scale
enhanced
ventral
recognition
patterns
cultivation.
Based
on
characteristics
enlarged
aggregate
size,
technical
cutting
subculture
proposed
improve
economic
feasibility.
Finally,
advantage
S-gel
maintaining
neurite
outgrowth
also
found,
which
suggests
its
potential
application
neural-related
microphysiological
systems.
Current Opinion in Neurobiology,
Год журнала:
2025,
Номер
92, С. 103018 - 103018
Опубликована: Апрель 11, 2025
The
field
of
brain
organoids
has
experienced
a
period
rapid
and
transformative
growth,
enabling
researchers
to
investigate
complex
human
biological
mechanisms
that
were
previously
deemed
intractable.
This
review
provides
an
overview
the
current
landscape
organoids,
with
particular
focus
on
their
relevance
in
context
neurodevelopmental
disorders.
It
also
emphasizes
crucial
role
these
models
play
elucidating
both
cell-autonomous
non-cell-autonomous
mechanisms.
We
describe
how
two
mechanisms,
often
considered
be
independent,
are
intricately
interconnected.
In
conclusion,
this
aims
highlight
utilization
considerably
advanced
our
comprehension
disorders,
while
delineating
prospective
avenues
for
investigating
conditions.
BMC Bioinformatics,
Год журнала:
2025,
Номер
26(1)
Опубликована: Апрель 15, 2025
Abstract
Background
Single-cell
RNA
sequencing
allows
for
the
exploration
of
transcriptomic
features
at
individual
cell
level,
but
high
dimensionality
and
sparsity
data
pose
substantial
challenges
downstream
analysis.
Feature
selection,
therefore,
is
a
critical
step
to
reduce
enhance
interpretability.
Results
We
developed
robust
feature
selection
algorithm
that
leverages
optimized
locally
estimated
scatterplot
smoothing
regression
(LOESS)
precisely
capture
relationship
between
gene
average
expression
level
positive
ratio
while
minimizing
overfitting.
Our
evaluations
showed
our
consistently
outperforms
eight
leading
methods
across
three
benchmark
criteria
helps
improve
analysis,
thus
offering
significant
improvement
in
subset
selection.
Conclusions
By
preserving
key
biological
information
through
GLP
provides
informative
accuracy
effectiveness
analyses.
Oncogene,
Год журнала:
2023,
Номер
42(32), С. 2417 - 2427
Опубликована: Июль 12, 2023
A
key
challenge
in
the
clinical
management
and
cause
of
treatment
failure
prostate
cancer
(PCa)
is
its
molecular,
cellular
heterogeneity.
Modelling
systems
that
fully
recapitulate
diversity
resistant
phenotypes
are
urgently
required
for
development
successful
personalised
PCa
therapies.
The
advent
three-dimensional
(3D)
organoid
model
has
revolutionised
preclinical
research
through
reflecting
heterogeneity
offering
genomic
environmental
manipulation
opened
up
unparalleled
opportunities
applications
disease
modelling,
high-throughput
drug
screening
precision
medicine.
Despite
these
remarkable
achievements
technology,
several
shortcomings
emulating
complex
tumor
microenvironment
dynamic
process
metastasis
as
well
epigenome
profile
limit
organoids
achieving
true
vivo
functionality.
Technological
advances
tissue
engineering
have
enabled
innovative
tools
to
facilitate
design
improved
3D
models.
In
this
review,
we
highlight
current
vitro
models
with
a
special
focus
on
discuss
approaches
create
more
physiologically
relevant
maximise
their
translational
relevance
ultimately
will
help
realise
transformational
power
Stem Cell Reports,
Год журнала:
2024,
Номер
19(11), С. 1635 - 1646
Опубликована: Окт. 10, 2024
Organoids,
self-organized
cell
aggregates,
contribute
significantly
to
developing
disease
models
and
cell-based
therapies.
Organoid-to-organoid
variations,
however,
are
inevitable
despite
the
use
of
latest
differentiation
protocols.
Here,
we
focused
on
morphology
organoids
formed
in
a
cerebral
organoid
culture
assessed
their
cellular
compositions
by
single-cell
RNA
sequencing
analysis.
The
data
revealed
that
primarily
composed
non-neuronal
cells,
such
as
those
from
neural
crest
choroid
plexus,
showed
unique
morphological
features.
Moreover,
demonstrate
non-destructive
analysis
can
accurately
distinguish
cortical
tissues
other
tissues,
thus
enhancing
experimental
accuracy
reliability
ensure
safety
