bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Дек. 21, 2021
Abstract
Currently
little
is
known
about
neuronal
positioning
and
the
roles
of
primary
cilia
in
postnatal
neurodevelopment.
We
show
that
principal
neurons
undergo
marked
changes
orientation,
concurrent
with
neuron
mouse
cerebral
cortex.
Primary
early-
late-born
compact
layers
display
opposite
orientations,
while
loose
laminae
are
predominantly
oriented
toward
pia.
In
contrast,
astrocytes
interneurons,
nucleated
brain
regions
do
not
specific
directionality.
further
discovered
cell
bodies
inside-out
laminated
spanning
from
hippocampal
CA1
region
to
neocortex
a
slow
“reverse
movement”
for
lamina
refinement.
Further,
selective
disruption
function
forebrain
leads
altered
lamination
gyrification
retrosplenial
cortex
(RSC)
formed
by
reverse
movement.
Collectively,
this
study
identifies
movement
as
fundamental
process
refines
casts
light
on
evolutionary
transition
3-layered
allocortices
6-layered
neocortices.
Summary
Statement
Guided
directionality,
we
somata
but
substantial
positioning,
which
cortical
layering.
Abstract
Primary
cilia
project
from
the
surface
of
most
vertebrate
cells
and
are
key
in
sensing
extracellular
signals
locally
transducing
this
information
into
a
cellular
response.
Recent
findings
show
that
primary
not
merely
static
organelles
with
distinct
lipid
protein
composition.
Instead,
function
relies
on
dynamic
composition
molecules
within
cilium,
context‐dependent
processing
stimuli,
cycles
assembly
disassembly
cell‐
tissue‐specific
manner.
Thereby,
dynamically
integrate
different
inputs
control
cell
fate
during
tissue
development.
Here,
we
review
recently
emerging
concept
dynamics
development,
organization,
remodeling,
function.
Trends in Neurosciences,
Год журнала:
2024,
Номер
47(5), С. 383 - 394
Опубликована: Апрель 4, 2024
Cilia
are
fascinating
organelles
that
act
as
cellular
antennae,
sensing
the
environment.
gained
significant
attention
in
late
1990s
after
their
dysfunction
was
linked
to
genetic
diseases
known
ciliopathies.
Since
then,
several
breakthrough
discoveries
have
uncovered
mechanisms
underlying
cilia
biogenesis
and
function.
Like
most
cells
animal
kingdom,
neurons
also
harbor
cilia,
which
enriched
neuromodulatory
receptors.
Yet,
how
neuronal
modulate
physiology
behavior
remains
poorly
understood.
By
comparing
ciliary
biology
between
sensory
central
nervous
systems
(CNS),
we
provide
new
perspectives
on
functions
of
brain
physiology.
Currently,
not
much
is
known
about
neuronal
positioning
and
the
roles
of
primary
cilia
in
postnatal
neurodevelopment.
We
show
that
principal
neurons
undergo
marked
changes
orientation,
concurrent
with
neuron
mouse
cerebral
cortex.
Primary
early-
late-born
compact
layers
display
opposite
orientations,
while
loose
laminae
are
predominantly
oriented
toward
pia.
In
contrast,
astrocytes
interneurons,
nucleated
brain
regions
do
specific
directionality.
further
discovered
cell
bodies
inside-out
laminated
spanning
from
hippocampal
CA1
region
to
neocortex
a
slow
'reverse
movement'
for
lamina
refinement.
Furthermore,
selective
disruption
function
forebrain
leads
altered
lamination
gyrification
retrosplenial
cortex
formed
by
reverse
movement.
Collectively,
this
study
identifies
movement
as
fundamental
process
refines
casts
light
on
evolutionary
transition
three-layered
allocortices
six-layered
neocortices.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 21, 2024
Abstract
Primary
cilia
are
a
critical
organelle
found
on
most
human
cells,
and
their
dysfunction
is
linked
to
hereditary
ciliopathies
with
diverse
symptoms.
Despite
significance,
the
specific
roles
of
in
different
cell
types
remain
poorly
understood
due
limitations
analyzing
ciliary
protein
composition.
We
employed
antibody-based
spatial
proteomics
expand
Human
Protein
Atlas
primary
cilia.
Our
analysis
identified
subciliary
locations
654
proteins
across
three
lines,
examining
110,844
individual
that
67%
proteome
cell-type
specific,
78%
exhibited
dynamically
varied
localization
patterns,
indicating
trafficking
crucial
for
function.
findings
portray
as
dynamic
sensors
tuning
effectively
sense
environment
compute
cellular
responses.
71
novel
genetic
variant
CREB3
typical
ciliopathy
phenotypes.
This
open,
atlas
advances
research
ciliopathies.
The
primary
cilium
serves
as
an
antenna
of
most
vertebrate
cells
and
is
important
for
conveying
cues
from
several
signaling
pathways
into
appropriate
cellular
responses
during
development
homeostasis.
Cilia
assembly
disassembly
processes
are
thought
to
be
strictly
controlled;
however,
the
precise
nature
molecular
events
underlying
this
control
still
awaits
full
resolution.
Through
their
enzymatic
activity,
kinases
function
flexible
yet
highly
controllable
regulators
a
vast
variety
processes.
Their
activity
ranges
cell
cycle
regulation
motility,
signal
transduction,
metabolism.
This
review
focuses
on
emerging
role
in
cilia
biology.
We
underscore
functions
formation,
maintenance,
resorption
while
examining
available
models
respective
mechanisms
actions.
Journal of Cellular Physiology,
Год журнала:
2025,
Номер
240(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Primary
cilia
are
polymodal
sensory
organelles
which
project
from
the
apical
side
of
polarized
cells.
They
found
in
all
brain
hemispheres
but
most
pronounced
neurons,
comprise
granular
layers
hippocampus
and
cerebellum.
Pathogenic
variants
genes
encode
primary
components
responsible
for
neuronal
ciliopathies—a
group
central
nervous
system
disorders
characterized
by
neurodevelopmental
conditions
such
as
intellectual
disability,
seizure,
ataxia,
deficits.
In
hippocampus,
form
chemical
synapses
with
axons
their
membranes
populated
unique
sets
ion
channels
G
protein‐coupled
receptors
(GPCRs).
small
privileged
compartments
that
challenging
to
study.
detail,
we
describe
electrophysiology
methods
use
cilia‐specific
fluorescent
sensors
assay
polycystin
channel
function
serotonergic
receptor
signaling,
respectively.
These
tools
allow
researchers
calcium,
cAMP
channel‐related
signaling
pathways
isolated
neurons
real‐time
semi‐quantitative
terms,
while
enhancing
our
understanding
this
understudied
organelle
its
dysregulation
ciliopathy
disease
states.
