Primary Cilia Directionality Reveals a Slow Reverse Movement of Principal Neurons for Postnatal Positioning and Lamina Refinement in the Cerebral Cortex DOI Open Access
Juan Yang,

Soheila Mirhosseiniardakani,

Liyan Qiu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Dec. 21, 2021

Abstract Currently little is known about neuronal positioning and the roles of primary cilia in postnatal neurodevelopment. We show that principal neurons undergo marked changes orientation, concurrent with neuron mouse cerebral cortex. Primary early- late-born compact layers display opposite orientations, while loose laminae are predominantly oriented toward pia. In contrast, astrocytes interneurons, nucleated brain regions do not specific directionality. further discovered cell bodies inside-out laminated spanning from hippocampal CA1 region to neocortex a slow “reverse movement” for lamina refinement. Further, selective disruption function forebrain leads altered lamination gyrification retrosplenial cortex (RSC) formed by reverse movement. Collectively, this study identifies movement as fundamental process refines casts light on evolutionary transition 3-layered allocortices 6-layered neocortices. Summary Statement Guided directionality, we somata but substantial positioning, which cortical layering.

Language: Английский

Emerging principles of primary cilia dynamics in controlling tissue organization and function DOI Creative Commons
Jay Gopalakrishnan, Kerstin Feistel, Benjamin M. Friedrich

et al.

The EMBO Journal, Journal Year: 2023, Volume and Issue: 42(21)

Published: Sept. 25, 2023

Abstract Primary cilia project from the surface of most vertebrate cells and are key in sensing extracellular signals locally transducing this information into a cellular response. Recent findings show that primary not merely static organelles with distinct lipid protein composition. Instead, function relies on dynamic composition molecules within cilium, context‐dependent processing stimuli, cycles assembly disassembly cell‐ tissue‐specific manner. Thereby, dynamically integrate different inputs control cell fate during tissue development. Here, we review recently emerging concept dynamics development, organization, remodeling, function.

Language: Английский

Citations

25
The neuronal cilium – a highly diverse and dynamic organelle involved in sensory detection and neuromodulation DOI Creative Commons
Nathalie Jurisch‐Yaksi, Dagmar Wachten, Jay Gopalakrishnan

et al.

Trends in Neurosciences, Journal Year: 2024, Volume and Issue: 47(5), P. 383 - 394

Published: April 4, 2024

Cilia are fascinating organelles that act as cellular antennae, sensing the environment. gained significant attention in late 1990s after their dysfunction was linked to genetic diseases known ciliopathies. Since then, several breakthrough discoveries have uncovered mechanisms underlying cilia biogenesis and function. Like most cells animal kingdom, neurons also harbor cilia, which enriched neuromodulatory receptors. Yet, how neuronal modulate physiology behavior remains poorly understood. By comparing ciliary biology between sensory central nervous systems (CNS), we provide new perspectives on functions of brain physiology.

Language: Английский

Citations

13
Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain DOI Creative Commons
Percival P. D’Gama, Inyoung Jeong, Andreas Moe Nygård

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(1), P. 115195 - 115195

Published: Jan. 1, 2025

Language: Английский

Citations

2
Cilia directionality reveals a slow reverse movement of principal neurons for positioning and lamina refinement in the cerebral cortex DOI Creative Commons
Juan Yang,

Soheila Mirhosseiniardakani,

Liyan Qiu

et al.

Development, Journal Year: 2025, Volume and Issue: 152(5)

Published: March 1, 2025

Currently, not much is known about neuronal positioning and the roles of primary cilia in postnatal neurodevelopment. We show that principal neurons undergo marked changes orientation, concurrent with neuron mouse cerebral cortex. Primary early- late-born compact layers display opposite orientations, while loose laminae are predominantly oriented toward pia. In contrast, astrocytes interneurons, nucleated brain regions do specific directionality. further discovered cell bodies inside-out laminated spanning from hippocampal CA1 region to neocortex a slow 'reverse movement' for lamina refinement. Furthermore, selective disruption function forebrain leads altered lamination gyrification retrosplenial cortex formed by reverse movement. Collectively, this study identifies movement as fundamental process refines casts light on evolutionary transition three-layered allocortices six-layered neocortices.

Language: Английский

Citations

1
Intrinsic Diversity in Primary Cilia Revealed Through Spatial Proteomics DOI Creative Commons
Jan N. Hansen, Huangqingbo Sun, Konstantin Kahnert

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract Primary cilia are a critical organelle found on most human cells, and their dysfunction is linked to hereditary ciliopathies with diverse symptoms. Despite significance, the specific roles of in different cell types remain poorly understood due limitations analyzing ciliary protein composition. We employed antibody-based spatial proteomics expand Human Protein Atlas primary cilia. Our analysis identified subciliary locations 654 proteins across three lines, examining 110,844 individual that 67% proteome cell-type specific, 78% exhibited dynamically varied localization patterns, indicating trafficking crucial for function. findings portray as dynamic sensors tuning effectively sense environment compute cellular responses. 71 novel genetic variant CREB3 typical ciliopathy phenotypes. This open, atlas advances research ciliopathies.

Language: Английский

Citations

4
Ciliogenesis defects after neurulation impact brain development and neuronal activity in larval zebrafish DOI Creative Commons

Percival P D'Gama,

Inyoung Jeong, Andreas Moe Nygård

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 110078 - 110078

Published: May 22, 2024

Language: Английский

Citations

4
Phosphorylation at the Helm: Kinase‐Mediated Regulation of Primary Cilia Assembly and Disassembly DOI Creative Commons
Andrea Lacigová, Lukáš Čajánek

Cytoskeleton, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

The primary cilium serves as an antenna of most vertebrate cells and is important for conveying cues from several signaling pathways into appropriate cellular responses during development homeostasis. Cilia assembly disassembly processes are thought to be strictly controlled; however, the precise nature molecular events underlying this control still awaits full resolution. Through their enzymatic activity, kinases function flexible yet highly controllable regulators a vast variety processes. Their activity ranges cell cycle regulation motility, signal transduction, metabolism. This review focuses on emerging role in cilia biology. We underscore functions formation, maintenance, resorption while examining available models respective mechanisms actions.

Language: Английский

Citations

0
Methods to Assess Neuronal Primary Cilia Electrochemical Signaling DOI Creative Commons
Paul G. DeCaen, Louise F. Kimura

Journal of Cellular Physiology, Journal Year: 2025, Volume and Issue: 240(4)

Published: April 1, 2025

ABSTRACT Primary cilia are polymodal sensory organelles which project from the apical side of polarized cells. They found in all brain hemispheres but most pronounced neurons, comprise granular layers hippocampus and cerebellum. Pathogenic variants genes encode primary components responsible for neuronal ciliopathies—a group central nervous system disorders characterized by neurodevelopmental conditions such as intellectual disability, seizure, ataxia, deficits. In hippocampus, form chemical synapses with axons their membranes populated unique sets ion channels G protein‐coupled receptors (GPCRs). small privileged compartments that challenging to study. detail, we describe electrophysiology methods use cilia‐specific fluorescent sensors assay polycystin channel function serotonergic receptor signaling, respectively. These tools allow researchers calcium, cAMP channel‐related signaling pathways isolated neurons real‐time semi‐quantitative terms, while enhancing our understanding this understudied organelle its dysregulation ciliopathy disease states.

Language: Английский

Citations

0
Metabolic reprogramming in polycystic kidney disease and other renal ciliopathies DOI Creative Commons
Sara Clerici, Alessandra Boletta

EMBO Molecular Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Language: Английский

Citations

0
Primary cilia in growth plates orchestrate long bone development DOI Creative Commons
Lei Zhang,

Xiaoqiao Xu,

Dike Tao

et al.

Fundamental Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0