Constructing and Tuning Excitatory Cholinergic Synapses: The Multifaceted Functions of Nicotinic Acetylcholine Receptors in Drosophila Neural Development and Physiology DOI Creative Commons
Justin Rosenthal, Quan Yuan

Frontiers in Cellular Neuroscience, Год журнала: 2021, Номер 15

Опубликована: Сен. 28, 2021

Nicotinic acetylcholine receptors (nAchRs) are widely distributed within the nervous system across most animal species. Besides their well-established roles in mammalian neuromuscular junctions, studies using invertebrate models have also proven fruitful revealing function of nAchRs central system. During earlier years, both vitro and had helped clarify basic molecular features members Drosophila nAchR gene family illustrated utility as targets for insecticides. Later, increasingly sophisticated techniques illuminated how mediate excitatory neurotransmission brain play an integral part neural development synaptic plasticity, well cognitive processes such learning memory. This review is intended to provide updated survey subunits, focusing on diversity unique contributions physiology plasticity fly circuitry. We will highlight promising new avenues research that likely contribute better understanding cholinergic other organisms.

Язык: Английский

Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle DOI Creative Commons
Max Jameson Aragon, Aaron T. Mok, Jamien Shea

и другие.

eLife, Год журнала: 2022, Номер 11

Опубликована: Янв. 24, 2022

We developed a multiphoton imaging method to capture neural structure and activity in behaving flies through the intact cuticle. Our measurements showed that fly head cuticle has surprisingly high transmission at wavelengths >900nm, difficulty of through-cuticle is due air sacs and/or fat tissue underneath By compressing or removing sacs, we performed brain anatomical functional results show 2- 3-photon are comparable superficial regions such as mushroom body, but superior deeper central complex beyond. further demonstrated 2-photon odor-evoked calcium responses from body γ-lobes short term long term. The here extends time limits vivo opens new ways brain.

Язык: Английский

Процитировано

30
Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications DOI Creative Commons
Sebastian Dunst, Pavel Tomančák

Genetics, Год журнала: 2018, Номер 211(1), С. 15 - 34

Опубликована: Ноя. 26, 2018

Abstract The development of fluorescent labels and powerful imaging technologies in the last two decades has revolutionized field fluorescence microscopy, which is now widely used diverse scientific fields from biology to biomedical materials science. Fluorescence microscopy also become a standard technique research laboratories working on Drosophila melanogaster as model organism. Here, we review principles wide-field Super-resolution its application field.

Язык: Английский

Процитировано

58
Olfactory and Neuromodulatory Signals Reverse Visual Object Avoidance to Approach in Drosophila DOI Creative Commons
Karen Y. Cheng,

Rachel A Colbath,

Mark A. Frye

и другие.

Current Biology, Год журнала: 2019, Номер 29(12), С. 2058 - 2065.e2

Опубликована: Май 30, 2019

Язык: Английский

Процитировано

46
Intellectual disability and autism spectrum disorders ‘on the fly’: insights from Drosophila DOI Creative Commons
Mireia Coll-Tané,

Alina Krebbers,

Anna Castells‐Nobau

и другие.

Disease Models & Mechanisms, Год журнала: 2019, Номер 12(5)

Опубликована: Май 1, 2019

Intellectual disability (ID) and autism spectrum disorders (ASD) are frequently co-occurring neurodevelopmental affect 2-3% of the population. Rapid advances in exome genome sequencing have increased number known implicated genes by threefold, to more than a thousand. The main challenges field now understand various pathomechanisms associated with this bewildering genetic disorders, identify new establish causality variants still-undiagnosed cases, work towards causal treatment options that so far available only for few metabolic conditions. To meet these challenges, research community needs highly efficient model systems. With an increasing relevant assays rapidly developing novel methodologies, fruit fly Drosophila melanogaster is ideally positioned change gear ID ASD research. aim Review summarize some exciting already has drawn attention as disorders. We highlight well-established ID- ASD-relevant phenotypes at (sub)cellular, brain behavioral levels, discuss strategies how extraordinarily versatile can contribute 'next generation' medical genomics better understanding

Язык: Английский

Процитировано

45
Rhodopsin-based voltage imaging tools for use in muscles and neurons of Caenorhabditis elegans DOI Creative Commons

Negin Azimi Hashemi,

Amelie Bergs,

Christina Schüler

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(34), С. 17051 - 17060

Опубликована: Авг. 1, 2019

Genetically encoded voltage indicators (GEVIs) based on microbial rhodopsins utilize the voltage-sensitive fluorescence of all- trans retinal (ATR), while in electrochromic FRET (eFRET) sensors, donor drops when rhodopsin acts as depolarization-sensitive acceptor. In recent years, such tools have become widely used mammalian cells but are less commonly invertebrate systems, mostly due to low yields. We systematically assessed Arch(D95N), Archon, QuasAr, and eFRET sensors MacQ-mCitrine QuasAr-mOrange, nematode Caenorhabditis elegans . ATR-bearing reported changes body wall muscles (BWMs), pharynx, feeding organ [where Arch(D95N) showed approximately 128% ΔF/F increase per 100 mV], neurons, integrating circuit activity. ATR is very dim, yet, using analog dimethylaminoretinal, it was boosted 250-fold. provided sensitivities 45 78% mV, induced by BWM action potentials, pharyngeal muscle, measured simultaneous optical sharp electrode recordings, 20% mV. All differences muscle depolarization a voltage-gated Ca 2+ -channel mutant. Optogenetically evoked de- or hyperpolarization motor neurons increased eliminated potential activity caused rise drop sensor fluorescence. Finally, we analyzed dynamics across entire showing uniform compartmentalized repolarization anterior posterior parts. Our work establishes all-optical, noninvasive electrophysiology live, intact C.

Язык: Английский

Процитировано

45
An electrically coupled pioneer circuit enables motor development via proprioceptive feedback in Drosophila embryos DOI Creative Commons

Xiangsunze Zeng,

Yuko Komanome,

Tappei Kawasaki

и другие.

Current Biology, Год журнала: 2021, Номер 31(23), С. 5327 - 5340.e5

Опубликована: Окт. 19, 2021

Язык: Английский

Процитировано

34
Dietary restriction and the transcription factor clock delay eye aging to extend lifespan in Drosophila Melanogaster DOI Creative Commons
Brian A. Hodge, Geoffrey T. Meyerhof, Subhash D. Katewa

и другие.

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Июнь 7, 2022

Abstract Many vital processes in the eye are under circadian regulation, and dysfunction has emerged as a potential driver of aging. Dietary restriction is one most robust lifespan-extending therapies amplifies rhythms with age. Herein, we demonstrate that dietary extends lifespan Drosophila melanogaster by promoting homeostatic protect visual system from age- light-associated damage. Altering positive limb core molecular clock transcription factor, CLOCK, or CLOCK-output genes, accelerates senescence, induces systemic immune response, shortens lifespan. Flies subjected to protected lifespan-shortening effects photoreceptor activation. Inversely, inactivation, achieved via mutating rhodopsin housing flies constant darkness, primarily reared on high-nutrient diet. Our findings establish diet-sensitive modulator indicates vision an antagonistically pleiotropic process contributes organismal

Язык: Английский

Процитировано

25
Context-dependent control of behavior in Drosophila DOI Creative Commons
Tess Oram, Gwyneth M Card

Current Opinion in Neurobiology, Год журнала: 2022, Номер 73, С. 102523 - 102523

Опубликована: Март 11, 2022

The representation of contextual information peripheral to a salient stimulus is central an animal's ability correctly interpret and flexibly respond that stimulus. While the computations circuits underlying context-dependent modulation stimulus-response pairings have typically been studied in vertebrates, genetic tractability, numeric simplification, well-characterized connectivity patterns Drosophila melanogaster brain facilitated circuit-level insights into processing. Recent studies flies reveal neuronal mechanisms create flexible behavioral responses sensory events conditions predation threat, feeding regulation, social interaction.

Язык: Английский

Процитировано

24
Spatial Comparisons of Mechanosensory Information Govern the Grooming Sequence in Drosophila DOI Creative Commons
Neil Zhang, Li Guo, J. Simpson

и другие.

Current Biology, Год журнала: 2020, Номер 30(6), С. 988 - 1001.e4

Опубликована: Март 1, 2020

Animals integrate information from different sensory modalities, body parts, and time points to inform behavioral choice, but the relevant comparisons underlying neural circuits are still largely unknown. We use grooming behavior of Drosophila melanogaster as a model investigate that govern motor sequence. Flies perform movements spontaneously, when covered with dust, they clean their bodies following an anterior-to-posterior After investigating modalities could detect we focus on mechanosensory bristle neurons, whose optogenetic activation induces similar Computational modeling predicts higher input strength head will cause anterior occur first. test this prediction using competition assay whereby two targeted light beams independently activate neurons parts. find initial choice movement is determined by ratio inputs In dust-covered flies, change result successful cleaning movements. Simulations our suggest results in sequence progression. One possibility flies frequent between posterior inputs, changing ratios drive choices. Alternatively, may track temporal given part measure effectiveness. The first hypothesis supported experiments: iterative spatial parts essential for organizing

Язык: Английский

Процитировано

39
PiVR: An affordable and versatile closed-loop platform to study unrestrained sensorimotor behavior DOI Creative Commons
David Tadres, Matthieu Louis

PLoS Biology, Год журнала: 2020, Номер 18(7), С. e3000712 - e3000712

Опубликована: Июль 14, 2020

Tools enabling closed-loop experiments are crucial to delineate causal relationships between the activity of genetically labeled neurons and specific behaviors. We developed Raspberry Pi Virtual Reality (PiVR) system conduct optogenetic stimulation neural functions in unrestrained animals. PiVR is an experimental platform that operates at high temporal resolution (70 Hz) with low latencies (<30 milliseconds), while being affordable (

Язык: Английский

Процитировано

39