Understanding bacterial biofilms: From definition to treatment strategies

Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13

Опубликована: Апрель 6, 2023

Bacterial biofilms are complex microbial communities encased in extracellular polymeric substances. Their formation is a multi-step process. Biofilms significant problem treating bacterial infections and one of the main reasons for persistence infections. They can exhibit increased resistance to classical antibiotics cause disease through device-related non-device (tissue) -associated infections, posing severe threat global health issues. Therefore, early detection search new alternative treatments essential suppressing biofilm-associated In this paper, we systematically reviewed biofilms, associated methods, potential treatment strategies, aiming provide researchers with latest progress biofilms.

Язык: Английский

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Inhibitors of bacterial H ₂ S biogenesis targeting antibiotic resistance and tolerance

Science, Год журнала: 2021, Номер 372(6547), С. 1169 - 1175

Опубликована: Июнь 10, 2021

Emergent resistance to all clinical antibiotics calls for the next generation of therapeutics. Here we report an effective antimicrobial strategy targeting bacterial hydrogen sulfide (H2S)-mediated defense system. We identified cystathionine γ-lyase (CSE) as primary generator H2S in two major human pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, discovered small molecules that inhibit CSE. These inhibitors potentiate bactericidal against both pathogens vitro mouse models infection. CSE also suppress tolerance, disrupting biofilm formation substantially reducing number persister bacteria survive antibiotic treatment. Our results establish a multifunctional factor drug target versatile enhancers.

Язык: Английский

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Environmental, Microbiological, and Immunological Features of Bacterial Biofilms Associated with Implanted Medical Devices

Clinical Microbiology Reviews, Год журнала: 2022, Номер 35(2)

Опубликована: Янв. 19, 2022

The spread of biofilms on medical implants represents one the principal triggers persistent and chronic infections in clinical settings, it has been subject many studies past few years, with most them focused prosthetic joint infections. We review here recent works biofilm formation microbial colonization a large variety indwelling devices, ranging from heart valves pacemakers to urological breast biliary stents endoscopic tubes contact lenses neurosurgical implants. focus bacterial abundance distribution across different devices body sites role environmental features, such as presence fluid flow properties implant surface, well interplay between response human immune system.

Язык: Английский

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Marine-derived polysaccharides and their therapeutic potential in wound healing application - A review

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 253, С. 127331 - 127331

Опубликована: Окт. 10, 2023

Язык: Английский

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Medical Device-Associated Biofilm Infections and Multidrug-Resistant Pathogens

Pathogens, Год журнала: 2024, Номер 13(5), С. 393 - 393

Опубликована: Май 8, 2024

Medical devices such as venous catheters (VCs) and urinary (UCs) are widely used in the hospital setting. However, implantation of these is often accompanied by complications. About 60 to 70% nosocomial infections (NIs) linked biofilms. The main complication ability microorganisms adhere surfaces form biofilms which protect them help persist host. Indeed, crossing skin barrier, insertion VC inevitably allows flora or accidental environmental contaminants access underlying tissues cause fatal complications like bloodstream (BSIs). In fact, 80,000 central catheters-BSIs (CVC-BSIs)-mainly occur intensive care units (ICUs) with a death rate 12 25%. Similarly, catheter-associated tract (CA-UTIs) most commonlyhospital-acquired (HAIs) worldwide.These represent up 40% NIs.In this review, we present summary biofilm formation steps. We provide an overview two important clinical settings medical devices, namely catheter-asociated (CA-BSIs) (CA-UTIs), highlight also multidrug resistant bacteria implicated infections. Furthermore, draw attention toseveral useful prevention strategies, advanced antimicrobial antifouling approaches developed reduce bacterial colonization on catheter incidence catheter-related

Язык: Английский

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Prevalence of Infections and Antimicrobial Resistance of ESKAPE Group Bacteria Isolated from Patients Admitted to the Intensive Care Unit of a County Emergency Hospital in Romania

Antibiotics, Год журнала: 2024, Номер 13(5), С. 400 - 400

Опубликована: Апрель 27, 2024

The ESKAPE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella Pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp.) is a of bacteria very difficult to treat due their high ability acquire resistance antibiotics and are the main cause nosocomial infections worldwide, posing threat global public health. Nosocomial with MDR found mainly in Intensive Care Units, multitude maneuvers invasive medical devices used, prolonged antibiotic treatments, serious general condition these critical patients, duration hospitalization. Materials Methods: During period one year, from January 2023 December 2023, this cross-sectional study was conducted on patients diagnosed sepsis admitted Unit Sibiu County Emergency Clinical Hospital. Samples taken were tracheal aspirate, catheter tip, pharyngeal exudate, wound secretion, urine culture, blood peritoneal fluid. Results: most common isolated our pneumoniae, followed by baumanii aeruginosa. Gram-positive cocci faecium Staphilococcus aureus) rarely isolated. Most bacteria. Conclusions: rise antimicrobial among strains environment especially Units raises concerns about limited treatment options.

Язык: Английский

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Carbonic anhydrase and bacterial metabolism: a chance for antibacterial drug discovery

Expert Opinion on Therapeutic Patents, Год журнала: 2024, Номер 34(6), С. 465 - 474

Опубликована: Март 20, 2024

Introduction Carbonic anhydrases (CAs, EC 4.2.1.1) play a pivotal role in the regulation of carbon dioxide , bicarbonate, and hydrogen ions within bacterial cells, ensuring pH homeostasis facilitating energy production. We conducted systematic literature search (PubMed, Web Science, Google Scholar) to examine intricate interplay between CAs metabolism, revealing potential CA inhibitors (CAIs) as innovative therapeutic agents against pathogenic bacteria.

Язык: Английский

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Biochemistry of Bacterial Biofilm: Insights into Antibiotic Resistance Mechanisms and Therapeutic Intervention

Life, Год журнала: 2025, Номер 15(1), С. 49 - 49

Опубликована: Янв. 2, 2025

Biofilms, composed of structured communities bacteria embedded in a self-produced extracellular matrix, pose significant challenge due to their heightened resistance antibiotics and immune responses. This review highlights the mechanisms underpinning antibiotic within bacterial biofilms, elucidating adaptive strategies employed by microorganisms withstand conventional antimicrobial agents. encompasses role altered gene expression, formation persister cells, contributing recalcitrance biofilms eradication. A comprehensive understanding these provides for exploring innovative therapeutic interventions. study explores promising avenues future research, emphasizing necessity uncovering specific genetic phenotypic adaptations occurring biofilms. The identification vulnerabilities biofilm architecture elucidation key biofilm-specific targets emerge as crucial focal points development targeted strategies. In addressing limitations traditional antibiotics, this discusses approaches. Nanomaterials with inherent properties, quorum-sensing inhibitors disrupting communication, bacteriophages viral agents are highlighted potential alternatives. exploration combination therapies, involving agents, biofilm-disrupting enzymes, immunomodulators, is emphasized enhance efficacy existing treatments overcome resilience.

Язык: Английский

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Biofilm Battle: New transformative tactics to tackle the bacterial biofilm infections

Microbial Pathogenesis, Год журнала: 2025, Номер 199, С. 107277 - 107277

Опубликована: Янв. 5, 2025

Язык: Английский

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Polymeric nano- and microparticulate drug delivery systems for treatment of biofilms

Advanced Drug Delivery Reviews, Год журнала: 2021, Номер 174, С. 30 - 52

Опубликована: Апрель 26, 2021

Язык: Английский

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