A comparative study of the neuroprotective effects of dl-3-n-butylphthalide and edaravone dexborneol on cerebral ischemic stroke rats

European Journal of Pharmacology, Год журнала: 2023, Номер 951, С. 175801 - 175801

Опубликована: Май 18, 2023

Язык: Английский

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Rho/ROCK Pathway and Noncoding RNAs: Implications in Ischemic Stroke and Spinal Cord Injury

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(21), С. 11573 - 11573

Опубликована: Окт. 26, 2021

Ischemic strokes (IS) and spinal cord injuries (SCI) are major causes of disability. RhoA is a small GTPase protein that activates downstream effector, ROCK. The up-regulation the RhoA/ROCK pathway contributes to neuronal apoptosis, neuroinflammation, blood-brain barrier dysfunction, astrogliosis, axon growth inhibition in IS SCI. Noncoding RNAs (ncRNAs), such as microRNAs (miRNAs) long noncoding (lncRNAs), were previously considered be non-functional. However, they have attracted much attention because play an essential role regulating gene expression physiological pathological conditions. There growing evidence ROCK inhibitors, fasudil VX-210, can reduce injury SCI animal models clinical trials. Recently, it has been reported miRNAs decreased SCI, while lncRNAs increased. Inhibiting Rho/ROCK with alleviates oxidative stress, Further studies required explore significance ncRNAs establish new strategies for preventing treating these devastating diseases.

Язык: Английский

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miR-195 Has a Potential to Treat Ischemic and Hemorrhagic Stroke through Neurovascular Protection and Neurogenesis

Molecular Therapy — Methods & Clinical Development, Год журнала: 2019, Номер 13, С. 121 - 132

Опубликована: Янв. 9, 2019

Tissue plasminogen activator is the only U.S. FDA-approved therapy for ischemic stroke, while there no specific medication hemorrhagic stroke. Therefore, treatment of acute stroke continues to be a major unmet clinical need. We explored effects miR-195 on neurovascular protection and its potential in treating Using both cellular animal studies, we showed that miR-195's beneficial are mediated by four mechanisms: (1) anti-apoptosis injured neural cells directly suppressing Sema3A/Cdc42/JNK signaling, (2) regeneration promoting stem cell proliferation migration, (3) anti-inflammation blocking NF-kB pathway, (4) improvement endothelial functions. intravenously injected carried nanoparticles into rats with either or stage. The results reduced size brain damage improved functional recovery types rats. reduction volume could up 45% approximately 30% therapeutic window between onset 6 h. Our data demonstrated possesses become new drug treat

Язык: Английский

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Noncoding RNA crosstalk in brain health and diseases

Neurochemistry International, Год журнала: 2021, Номер 149, С. 105139 - 105139

Опубликована: Июль 16, 2021

Язык: Английский

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Long non-coding RNA H19 expression and functional polymorphism rs217727 are linked to increased ischemic stroke risk

BMC Neurology, Год журнала: 2021, Номер 21(1)

Опубликована: Фев. 4, 2021

Abstract Background Efforts to identify potential biomarkers for the diagnosis of ischemic stroke (IS) are valuable. The H19 gene plays a functional role in increasing prevalence IS risk factors. We evaluated correlation between rs217727 polymorphism and expression level lncRNA with susceptibility among Iranian population. Methods Blood samples were collected from patients ( n = 114) controls 114). concentrated on pattern at different time points (i.e., 0–24, 24–48, 48–72 h after stroke). tetra-amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) method was applied DNA genotyping. used quantitative real-time PCR evaluate levels. receiver operating characteristic (ROC) curve prognosis IS. Results rs217727polymorphism related co-dominant (OR 2.92, 95% CI 0.91–10.92, P 0.04) recessive models 2.80, 0.96–8.15, 0.04). significantly upregulated remained high 72 stroke. ROC curves showed that within first 24 onset might serve as biomarker early 79.49% sensitivity 80.00% specificity. small vessel occlusion (SVO) large-artery atherosclerosis (LAA) 3.74 3.34 times higher than undetermined (UD) subtype, respectively [OR CL (1.14–12.27) 0.030 OR (1.13–9.85) 0.029]. Conclusion is correlated susceptibility, levels SVO LAA patients. upregulation may be considered diagnostic population, but it cannot useful prognostic marker.

Язык: Английский

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MiRNA: Involvement of the MAPK Pathway in Ischemic Stroke. A Promising Therapeutic Target

Medicina, Год журнала: 2021, Номер 57(10), С. 1053 - 1053

Опубликована: Окт. 1, 2021

Ischemic stroke (IS) is a cerebrovascular disease with high rate of disability and mortality. It classified as the second leading cause death that arises from sudden occlusion small vessels in brain consequent lack oxygen nutrients tissue. Following an acute ischemic event, cascade events promotes activation multiple signaling pathways responsible for irreversible neuronal damage. The mitogen-activated protein kinase (MAPK) pathway transmits signals cell membrane to nucleus response different stimuli, regulating proliferation, differentiation, inflammation, apoptosis. Several lines evidence showed MAPK important regulator hemorrhagic cerebral vascular disease; indeed, it can impair blood–brain barrier (BBB) integrity exacerbate neuroinflammation through release pro-inflammatory mediators implementing neurovascular damage after stroke. This review aims illustrate miRNAs involved regulation IS, order highlight possible targets potential neuroprotective treatments. We also discuss some (miR), including miR-145, miR-137, miR-493, miR-126, are they modulate processes such apoptosis, neuroinflammation, neurogenesis, angiogenesis IS. To date, limited drug therapies available treatment IS; therefore, necessary implement preclinical clinical studies aimed at discovering novel therapeutic approaches minimize post-stroke neurological

Язык: Английский

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LncRNAs Stand as Potent Biomarkers and Therapeutic Targets for Stroke

Frontiers in Aging Neuroscience, Год журнала: 2020, Номер 12

Опубликована: Окт. 19, 2020

Stroke is a major public health problem worldwide with high burden of neurological disability and mortality. Long noncoding RNAs (lncRNAs) have attracted much attention in the past decades because their newly discovered roles pathophysiological processes many diseases. The abundance lncRNAs nervous system indicates that they may be part complex regulatory network governing physiology pathology brain. In particular, been shown to play pivotal pathogenesis stroke. this article, we provide review multifaceted functions ischemic stroke intracerebral hemorrhage, highlighting promising use as diagnostic biomarkers therapeutics. To end, discuss potential stem cells aiding lncRNA applications

Язык: Английский

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LncRNA MACC1-AS1 attenuates microvascular endothelial cell injury and promotes angiogenesis under hypoxic conditions via modulating miR-6867-5p/TWIST1 in human brain microvascular endothelial cells

Annals of Translational Medicine, Год журнала: 2020, Номер 8(14), С. 876 - 876

Опубликована: Июль 1, 2020

Hypoxia following ischemic stroke is a common cause of brain insults. Mounting evidence suggests that long non-coding RNAs (lncRNAs) play vital role in regulating certain physiological and pathological processes including stroke. For the first time, present study investigated effects mechanism LncRNA MACC1-AS1 on hypoxia-induced human microvascular endothelial cells (HBMECs).LncRNA levels HBMECs were detected via reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay. Reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), using their respective kits. Flow cytometry clone formation assay performed to evaluate lncRNA cell apoptosis proliferation respectively. Angiogenesis capacity was evaluated tube Transwell migration for assessment migration, Western blot measurement Twist1 VE-cadherin level, permeability evaluation barrier function. The target gene predicted bioinformatics online tool validated through luciferase reporter RNA pull-down assay.LncRNA downregulated HBMECs. Overexpression reduced oxidative stress, while promoting proliferation, angiogenesis. Moreover, overexpression elevated which contributed maintaining TWIST1 as miR-6867-5p further targeted by MACC1-AS1. Thus, functions miR-6867-5p/TWIST1.In this study, we found exerted protective miR-6867-5p/TWIST1, indicating new therapeutic strategy future therapy.

Язык: Английский

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LncRNA MEG3: Potential stock for precision treatment of cardiovascular diseases

Frontiers in Pharmacology, Год журнала: 2022, Номер 13

Опубликована: Ноя. 29, 2022

The prevalence and mortality rates of cardiovascular diseases are increasing, new treatment strategies urgently needed. From the perspective basic pathogenesis, occurrence development related to inflammation, apoptosis, fibrosis autophagy cardiomyocytes, endothelial cells other cells. involvement maternally expressed gene 3 (MEG3) in human disease processes has been increasingly reported. P53 PI3K/Akt important pathways by which MEG3 participates regulating cell apoptosis. directly or competitively binds with miRNA participate oxidative stress, endoplasmic reticulum EMT processes. LncRNA is mainly involved malignant tumors, metabolic diseases, immune system cerebrovascular etc., a variety pathological effects fibroblasts great clinical application potential prevention AS, MIRI, hypertension HF. This paper will review research progress aspects mechanism action, systemic point out its diseases. lncRNAs also play role In addition, lncRNA shown biomarker value, prognostic value therapeutic response measurement tumor We boldly speculate that emerging discipline heart disease.

Язык: Английский

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Scutellarin Acts via MAPKs Pathway to Promote M2 Polarization of Microglial Cells

Molecular Neurobiology, Год журнала: 2023, Номер 60(8), С. 4304 - 4323

Опубликована: Апрель 22, 2023

Язык: Английский

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