Acta veterinaria,
Год журнала:
2023,
Номер
73(4), С. 486 - 501
Опубликована: Дек. 1, 2023
Abstract
Alterations
in
the
serum
protein
pattern
may
be
associated
with
many
diseases,
including
neoplastic
processes.
In
veterinary
medicine,
these
changes
are
poorly
understood.
Therefore,
this
study
was
aimed
at
analysis
of
distribution
blood
fractions
separated
by
agarose
gel
electrophoresis,
and
determination
concentrations
main
acute
phase
proteins
bitches
mammary
gland
neoplasia.
The
evaluation
conducted
on
twelve
female
dogs
palpable
single
or
multiple
nodules
parenchyma
ten
tumor-free
clinically
healthy
to
compare
possible
differences
obtained
results.
Blood
used
perform
electrophoresis
analyze
total
following
canine
proteins:
amyloid
A,
haptoglobin,
C-reactive
α
1
-acid
glycoprotein.
were
slightly
higher
tumors.
Serum
showed
lower
mean
albumin
-globulins
affected
dogs,
while
2
-and
β
significantly
(P=0.0032
P=0.0021,
respectively)
compared
without
proteins,
haptoglobin
tumors
(P=0.0025
P=0.0002,
respectively).
values
glycoprotein
did
not
vary
markedly
between
Presented
data
suggest
that
processes
also
alter
electrophoretic
induce
production
some
inflammatory
proteins.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 10195 - 10195
Опубликована: Сен. 23, 2024
Ovarian
cancer
(OC),
particularly
high-grade
serous
carcinoma
(HGSC),
is
a
leading
cause
of
gynecological
mortality
due
to
late
diagnosis
and
chemoresistance.
While
studies
on
OC
cell
lines
have
shown
that
overexpression
the
ATP7A
membrane
transporter
correlates
with
resistance
platinum-based
drugs
(PtBMs)
cross-resistance
copper
(Cu),
clinical
evidence
lacking.
The
functionality
ceruloplasmin
(CP),
main
Cu-transporting
protein
in
blood,
dependent
on,
among
other
things,
activity.
This
study
investigated
expression
CP
levels
as
potential
biomarkers
for
predicting
responses
PtBMs.
We
included
28
HGSC
patients
who
underwent
neoadjuvant
chemotherapy
(NACT).
ovarian
peritoneal
tissues
before
NACT
omental
after
was
analyzed
via
qPCR,
ascites
plasma
were
measured
ELISA
NACT.
In
total,
54%
exhibited
pretreatment
tissue
(ovary
and/or
peritoneum),
while
43%
treatment
(peritoneum
omentum).
A
significant
association
found
between
higher
peritoneum
an
unfavorable
CA-125
elimination
rate
constant
k
(KELIM)
score.
Patients
had
significantly
mean
Plasma
decreased
NACT,
associated
shorter
platinum-free
interval
(PFI).
These
findings
suggest
serve
predictive
markers
chemoresistance,
but
further
research
needed
validate
their
utility.
Journal of Analytical Atomic Spectrometry,
Год журнала:
2022,
Номер
37(8), С. 1675 - 1686
Опубликована: Янв. 1, 2022
A
new
method
based
on
conjoint
liquid
chromatography
with
post-column
ID-ICP-MS
detection
was
developed
for
the
speciation
of
Cu
in
human
serum.
ceruloplasmin
(Cu-Cp)
is
suggested
as
a
potential
biomarker
cancer
diagnosis.
Aging,
Год журнала:
2022,
Номер
14(1), С. 73 - 108
Опубликована: Янв. 11, 2022
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
has
rapidly
spread
around
the
world
and
became
a
global
pandemic
in
2020.
One
promising
drug
target
for
SARS-CoV-2
is
transmembrane
protease
serine
(TMPRSS2).
This
study
was
designed
to
explore
expression
status,
prognostic
significance
molecular
functions
of
TMPRSS2
lung
cancer.
investigated
using
TIMER,
Oncomine,
UALCAN,
GEO,
HPA
TCGA
databases.
The
value
examined
Cox
regression
nomogram.
KEGG,
GO
GSEA
were
performed
investigate
cellular
function
relationship
between
immune
infiltration
determined
TIMER
CIBERSORT
algorithms.
mRNA
protein
significantly
reduced
Decreased
increased
DNA
methylation
associated
with
various
clinicopathological
parameters
patients
Low
also
correlated
poor
outcome
cancer
patients.
Moreover,
nomogram
constructed
exhibited
good
predictive
power
overall
survival
KEGG
analyses
implied
that
multiple
immune-
metabolism-related
pathways
linked
expression.
Intriguingly,
cell
More
importantly,
markedly
decreased
SARS-CoV-infected
cells.
These
findings
indicate
may
be
biomarker
therapeutic
through
metabolic
infiltration.
Mediators of Inflammation,
Год журнала:
2023,
Номер
2023, С. 1 - 12
Опубликована: Фев. 13, 2023
Patients
with
low-grade
glioma
(LGG)
have
wildly
varying
average
lifespans.
However,
no
effective
way
exists
for
identifying
LGG
patients
at
high
risk.
Cuproptosis
is
a
recently
described
form
of
cell
death
associated
the
abnormal
aggregation
lipid
acylated
proteins.
Few
investigations
been
conducted
on
cuproptosis-associated
genes
and
thus
far.
The
purpose
this
research
to
establish
predictive
model
cuproptosis-related
in
order
recognise
populations
risk.We
analyzed
926
LGGs
from
2
public
datasets,
all
which
were
RNA
sequencing
datasets.
On
basis
immune
scores,
population
was
split
into
different
risk
categories
X-tile.
LASSO
Cox
regressions
employed
filter
construct
prediction
models.
accuracy
models
measured
by
using
TCGA
internal
validation
set
CGGA
external
set.
In
addition,
infiltration
viewed
CIBERSORT
ssGSEA
algorithms
correlation
analysis
done
genes.
Finally,
escape
capacity
low-
high-risk
groups
evaluated
TIDE
method.The
constructed
four
used
identify
LGG.
It
performed
well
training
sets
(AUC
values:
0.915,
0.894,
0.774).
Meanwhile,
we
found
that
FDX1
ATP7A
positively
correlated
response,
while
GCSH
ATP7B
opposite.
score
group
had
lower
score,
indicating
their
weak.High-risk
individuals
can
be
reliably
identified
based
Furthermore,
cuproptosis
closely
related
tumor
microenvironment,
gives
novel
approach
treating
Cancers,
Год журнала:
2023,
Номер
16(1), С. 81 - 81
Опубликована: Дек. 22, 2023
Chronic
inflammatory
processes
are
related
to
all
stages
of
tumorigenesis.
As
inflammation
is
closely
associated
with
the
activation
and
release
different
cytotoxic
agents,
interplay
between
agents
antagonizing
principles
highlighted
in
this
review
address
question
how
tumor
cells
overcome
enhanced
values
tumors.
In
cells,
formation
mitochondrial-derived
reactive
species
elevated
iron
ions
free
heme
antagonized
by
an
overexpression
enzymes
proteins,
contributing
antioxidative
defense
maintenance
redox
homeostasis.
Through
these
mechanisms,
can
even
survive
additional
stress
caused
radio-
chemotherapy.
secretion
active
from
immune
suppressed
microenvironment
extracellular
matrix
components
induced.
Different
oxidant-
protease-based
involved
tumor-mediated
immunosuppression,
growth,
cell
invasion,
metastasis.
Considering
special
metabolic
conditions
tumors,
main
focus
here
was
directed
on
disturbed
balance
protective
mechanisms
late-stage
This
knowledge
mandatory
for
implementation
novel
anti-cancerous
therapeutic
approaches.
BMC Veterinary Research,
Год журнала:
2024,
Номер
20(1)
Опубликована: Июнь 29, 2024
Abstract
Background
Feline
mammary
carcinoma
(FMC)
is
a
common
aggressive
and
highly
metastatic
cancer
affecting
female
cats.
Early
detection
essential
for
preventing
local
distant
metastasis,
thereby
improving
overall
survival
rates.
While
acquiring
molecular
data
before
surgery
offers
significant
potential
benefits,
the
current
protein
biomarkers
monitoring
disease
progression
in
non-metastatic
FMC
(NmFMC)
(mFMC)
are
limited.
The
objective
of
this
study
was
to
investigate
serum
peptidome
profiles
NmFMC
mFMC
using
liquid
chromatography-tandem
mass
spectrometry.
A
cross-sectional
conducted
compare
13
NmFMC,
23
18
healthy
spectrometry
analysis
performed
on
non-trypsinized
samples.
Results
Out
total
8284
expressed
proteins
observed,
several
were
found
be
associated
with
human
breast
cancer.
In
distinctive
expressions
encompassed
double-stranded
RNA-binding
Staufen
homolog
2
(STAU2),
cell
proliferation,
along
bromodomain
adjacent
zinc
finger
domain
2A
(BAZ2A)
gamma-aminobutyric
acid
type
receptor
subunit
epsilon
(GABRE),
identified
as
treatment
targets.
Paradoxically,
positive
prognostic
markers
emerged,
such
complement
C1q
like
3
(C1QL3)
erythrocyte
membrane
band
4.1
(EPB41
or
4.1R).
Within
group,
overexpressed
poor
prognosis
exhibited,
including
B-cell
lymphoma
6
transcription
repressor
(BCL6),
thioredoxin
reductase
(TXNRD3)
ceruloplasmin
(CP).
Meanwhile,
presence
POU
class
5
homeobox
(POU5F1
OCT4)
laminin
alpha
1
(LAMA1),
reported
biomarkers,
noted.
Conclusion
both
pro-
anti-proliferative
potentially
indicating
characteristic
NmFMC.
Conversely,
metastasis
noted
group.
International Journal of Oncology,
Год журнала:
2022,
Номер
61(6)
Опубликована: Окт. 12, 2022
Breast
cancer
is
the
most
prevalent
type
of
among
women
worldwide.
The
heterogeneous
nature
breast
poses
a
serious
challenge
for
prognostic
prediction
and
individualized
therapies.
Recently,
ferroptosis,
an
iron‑dependent
form
programmed
cell
death,
has
been
reported
to
serve
significant
role
in
regulation
biological
behavior
tumors.
Several
studies
have
revealed
significance
ferroptosis‑related
gene
(FRG)
model;
however,
additional
efforts
are
required
elucidate
details.
Moreover,
genes
that
modulate
ferroptosis
may
be
promising
candidate
bioindicators
therapy.
present
study
systematically
assessed
expression
profiles
FRGs
reveal
relationship
between
features
patients
with
based
on
data
obtained
from
Gene
Expression
Omnibus
Molecular
Taxonomy
Cancer
International
Consortium.
Using
non‑negative
matrix
factorization
clustering
method,
were
classified
into
two
sub‑groups
(cluster
1
cluster
2)
FRGs.
Furthermore,
Cox
regression,
least
absolute
shrinkage
selection
operator
methods
used
construct
risk
score
formula
comprised
nine
genes,
which
stratified
groups.
Patients
belonging
high‑risk
group
exhibited
significantly
shorter
overall
survival
(OS)
time
compared
low‑risk
group.
value
this
signature
was
further
verified
training
validation
cohorts.
results
univariate
multivariate
regression
analyses
indicated
acted
as
independent
predictor
OS.
Subsequently,
nomogram
constructed.
Receiver
operating
characteristic
analysis
confirmed
resulting
powerful
discriminatory
ability.
Functional
immune
environment
differed
notably
groups
association
proliferation,
migration
drug
resistance.
Taken
together,
demonstrated
associated
progression,
thus
could
novel
biomarkers
treatment
cancer.
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 28, 2023
Abstract
Background
Nullomers
are
the
shortest
strings
of
absent
amino
acid
(aa)
sequences
in
a
species
or
group
species.
Primes
those
nullomers
that
have
not
been
detected
genome
any
9S1R
is
5-aa
peptide
derived
from
prime
sequence
tagged
with
5
arginine
aa,
used
to
treat
triple
negative
breast
cancer
(TNBC)
an
vivo
TNBC
mouse
model.
administered
trehalose
(9S1R-NulloPT),
which
enhances
solubility
and
exhibits
some
independent
effects
against
tumor
growth
thus
important
component
drug
preparation.
Method
We
examined
effect
9S1R-NulloPT
on
growth,
metabolism,
metastatic
burden,
necrosis,
immune
microenvironment,
transcriptome
aggressive
tumors.
Results
The
peptide-treated
mice
had
smaller
tumors
initial
phase
treatment,
as
compared
untreated
control,
reduced
bioluminescence
at
later
stages,
indicative
metabolically
inactive
A
decrease
ex
was
also
observed
excised
treated
mice,
but
secondary
metastasis
lungs.
treatment
caused
changes
microenvironment
increased
infiltration
cells
margin
inflammation.
upregulated
365
genes
downregulated
710
group.
Consistent
vitro
findings
cell
lines,
include
Cellular
Metabolic
Process
Related
(179),
specifically
mitochondrial
associated
TCA
cycle/oxidative
phosphorylation
(44),
translation
machinery/ribosome
biogenesis
(45).
Among
genes,
Developmental
Pathway
(13),
ECM
Organization
(12)
Focal
Adhesion
Pathways
(7)
were
noteworthy.
present
data
pilot
study
using
bilateral
BC
model,
supports
our
findings.
Conclusion
In
conclusion,
although
moderate
reducing
volume,
it
altered
well
transcriptome,
rendering
less
active
by
downregulating
function
ribosome
biogenesis.
This
corroborates
previously
published
Current Issues in Molecular Biology,
Год журнала:
2023,
Номер
45(10), С. 8502 - 8518
Опубликована: Окт. 22, 2023
Cuproptosis
induction
is
seen
as
a
promising
alternative
for
immunotherapies
and
targeted
therapies
in
breast
cancer.
The
objective
of
this
research
was
to
examine
the
prognostic
biological
importance
cuproptosis-related
genes
(CRGs)
lung
adenocarcinoma
(LUAD).The
following
methods
were
used:
GSE10072
dataset
TCGA
database
analysis,
differential
expression
analysis
CRGs,
function
(BP)
signaling
pathway
enrichment
clinical
construction
signature
RNA
modified
miRNA
CRGs
LUAD,
immunoinfiltration
immunohistochemical
staining
DβH,
UBE2D3,
SOD1,
UBE2D1
LOXL2.AOC1,
ATOX1,
CCL8,
CCS,
COX11,
CP,
LOXL2,
MAP2K2,
PDK1,
SCO2,
UBE2D1,
UBE2D3
VEGFA
showed
significantly
higher
expression,
while
ATP7B,
PDE3B,
SLC31A2,
UBE2D2,
UBE2D4
ULK2
lower
LUAD
tissues
than
normal
tissues.
We
also
found
that
ATP7B
(4%),
AOC1
(3%)
PDE3B
(2%),
DβH
CP
(1%),
PDK1
LOXL2
(1%)
mutation
frequencies.
univariate
Cox
used
identify
have
value.
It
identified
21
significant
value,
containing
LOXL2.
Patients
with
up-expression
longer
survival
time
patients
down-expression
time.
hsa-miR-29c-3p,
hsa-miR-29a-3p,
hsa-miR-181c-5p,
hsa-miR-1245a,
etc.,
play
an
important
role
regulatory
network,
miR-29a,
miR-29c
miR-181c
high
longer,
miR-1245a
low
longer.
performed
relationships
between
immune
infiltration
including
B
cells,
CD8+
T
CD4+
macrophages,
neutrophils
DCs.DβH,
are
potential
candidates
implicated
can
be
further
explored
their
application
diagnostic,
prognostic,
therapeutic
biomarkers
LUAD.