Gut
microbiota,
which
act
as
a
determinant
of
pharmacokinetics,
have
long
been
overlooked.
In
recent
years,
growing
body
evidence
indicates
that
the
gut
microbiota
influence
drug
metabolism
and
efficacy.
Conversely,
drugs
also
exert
substantial
on
function
composition
microbiota.
Pharmacomicrobiomics,
an
emerging
field
focusing
interplay
provides
potential
foundation
for
making
certain
advances
in
personalized
medicine.
Understanding
communication
between
antiparkinsonian
is
critical
precise
treatment
Parkinson's
disease.
Here,
we
provide
historical
overview
drugs.
Moreover,
discuss
mechanistic
insights
into
complex
associations
metabolism.
addition,
draw
attention
to
microbiota-based
biomarkers
predicting
efficacy
examine
current
state-of-the-art
knowledge
strategies
optimize
therapy
Trends in biotechnology,
Год журнала:
2023,
Номер
41(8), С. 1000 - 1012
Опубликована: Март 30, 2023
Clustered
regularly
interspaced
short
palindromic
repeats-associated
protein
9
(CRISPR–Cas)-mediated
genome
editing
has
revolutionized
biomedical
research
and
will
likely
change
the
therapeutic
diagnostic
landscape.
However,
CRISPR–Cas9,
which
edits
DNA
by
activating
double-strand
break
(DSB)
repair
pathways,
is
not
always
sufficient
for
gene
therapy
applications
where
precise
mutation
required.
Prime
editing,
latest
revolution
in
genome-editing
technologies,
can
achieve
any
possible
base
substitution,
insertion,
or
deletion
without
requirement
DSBs.
prime
still
its
infancy,
further
development
needed
to
improve
efficiency
delivery
strategies
applications.
We
summarize
developments
optimization
of
editor
(PE)
variants
with
improved
precision.
Moreover,
we
highlight
some
potential
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Май 12, 2022
The
Clustered
Regularly
Interspaced
Short
Palindromic
Repeat
(CRISPR)
and
its
accompanying
protein
(Cas9)
are
now
the
most
effective,
efficient,
precise
genome
editing
techniques.
Two
essential
components
of
CRISPR/Cas9
system
guide
RNA
(gRNA)
CRISPR-associated
proteins.
Choosing
implementing
safe
effective
delivery
systems
in
therapeutic
application
has
proven
to
be
a
significant
problem.
For
vivo
delivery,
viral
vectors
natural
specialists.
Due
their
higher
effectiveness
than
other
methods,
such
as
adenoviral
(AdVs),
adeno-associated
viruses
(AAVs),
lentivirus
(LVs)
commonly
employed
methods.
This
review
thoroughly
examined
recent
achievements
using
variety
means
well
benefits
limitations
each
vector.
Future
thoughts
for
overcoming
current
restrictions
adapting
technology
also
discussed.
Pharmaceuticals,
Год журнала:
2023,
Номер
16(10), С. 1416 - 1416
Опубликована: Окт. 5, 2023
In
the
realm
of
gene
therapy,
a
pivotal
moment
arrived
with
Paul
Berg’s
groundbreaking
identification
first
recombinant
DNA
in
1972.
This
achievement
set
stage
for
future
breakthroughs.
Conditions
once
considered
undefeatable,
like
melanoma,
pancreatic
cancer,
and
host
other
ailments,
are
now
being
addressed
at
their
root
cause—the
genetic
level.
Presently,
therapy
landscape
stands
adorned
22
approved
vivo
ex
products,
including
IMLYGIC,
LUXTURNA,
Zolgensma,
Spinraza,
Patisiran,
many
more.
this
comprehensive
exploration,
we
delve
into
rich
assortment
16
drugs,
from
siRNA,
miRNA,
CRISPR/Cas9
to
aptamers
TRAIL/APO2L,
as
well
46
carriers,
AAV,
AdV,
LNPs,
exosomes
naked
mRNA,
sonoporation,
magnetofection.
The
article
also
discusses
advantages
disadvantages
each
product
vector
type,
current
challenges
faced
practical
use
its
potential.
Journal of Drug Delivery Science and Technology,
Год журнала:
2024,
Номер
92, С. 105338 - 105338
Опубликована: Янв. 6, 2024
The
rapid
advancement
of
CRISPR-Cas9
technology
has
instigated
a
profound
transformation
in
genome
editing
with
significant
implications
for
fields
like
health,
agriculture,
and
biotechnology.
This
abstract
provides
an
overview
the
historical
significance
fundamental
components
CRISPR-Cas9,
notably
Cas9
protein
guide
RNA,
underscoring
its
pivotal
role
genetic
manipulation.
It
emphasizes
CRISPR-Cas9's
preeminence
domain
precise
editing,
driving
breakthroughs
personalized
medicine,
gene
therapy,
agriculture.
Of
paramount
importance
is
integration
nanomaterials,
encompassing
lipid-based
polymeric
nanoparticles,
alongside
viral
vectors,
serving
as
potent
vehicles
augmenting
delivery
efficiency
precision.
We
explore
strategies
aimed
at
enhancing
through
while
also
addressing
ethical
regulatory
considerations.
In
expert
opinion
section,
we
offer
nuanced
perspective
on
present
state
field,
highlighting
potential
transformative
progress
research
therapy.
stands
brink
unlocking
new
possibilities
providing
innovative
solutions
to
address
pressing
global
challenges.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 28, 2023
Fungal
lipases
(triacylglycerol
acyl
hydrolases
EC
3.1.1.3)
are
significant
industrial
enzymes
and
have
several
applications
in
a
number
of
industries
fields.
found
species
fungi
yeast.
These
carboxylic
acid
esterases,
categorized
under
the
serine
hydrolase
family,
do
not
require
any
cofactor
during
catalyzing
reactions.
It
was
also
noticed
that
processes
including
extraction
purification
from
comparatively
easier
cheaper
than
other
sources
lipases.
In
addition,
fungal
been
classified
into
three
chief
classes,
namely,
GX,
GGGX,
Y.
only
hydrolysis
fats
oils
(triglycerides)
but
involved
synthetic
reactions
such
as
esterification,
acidolysis,
alcoholysis,
interesterification,
aminolysis.
The
production
activity
highly
affected
by
carbon
source,
nitrogen
temperature,
pH,
metal
ions,
surfactants,
moisture
content.
Therefore,
biotechnological
many
fields
biodiesel
production,
ester
synthesis,
biodegradable
biopolymers,
formulations
cosmetics
personal
care
products,
detergent
manufacturing,
degreasing
leather,
pulp
paper
textile
industry,
biosensor
development,
drug
diagnostic
tool
medical
sector,
biodegradation
esters,
bioremediation
wastewater.
immobilization
onto
different
carriers
helps
improving
catalytic
activities
efficiencies
increasing
thermal
ionic
stability
(in
organic
solvents,
high
temperature),
being
easy
to
recycle,
inducing
volume-specific
loading
enzyme
support,
thus,
these
features
proved
be
appropriate
for
use
biocatalysts
sectors.
Nucleic Acid Therapeutics,
Год журнала:
2024,
Номер
34(2), С. 52 - 72
Опубликована: Март 20, 2024
Nucleic
acid-based
therapies
have
become
the
third
major
drug
class
after
small
molecules
and
antibodies.
The
role
of
nucleic
has
been
strengthened
by
recent
regulatory
approvals
tremendous
clinical
success.
In
this
review,
we
look
at
obstacles
that
hindered
field,
historical
milestones
achieved,
what
is
yet
to
be
resolved
anticipated
soon.
This
review
provides
a
view
key
innovations
are
expanding
acid
capabilities,
setting
stage
for
future
therapeutics.
Biologics,
Год журнала:
2024,
Номер
Volume 18, С. 21 - 28
Опубликована: Янв. 1, 2024
Abstract:
The
CRISPR-Cas
(Clustered
Regularly
Interspaced
Short
Palindromic
Repeat
(CRISPR))
and
the
associated
protein
(Cas9)
system,
a
young
but
well-studied
genome-editing
tool,
holds
plausible
solutions
to
wide
range
of
genetic
disorders.
single-guide
RNA
(sgRNA)
with
20-base
user-defined
spacer
sequence
Cas9
endonuclease
form
core
CRISPR-Cas9
system.
This
sgRNA
can
direct
nuclease
any
genomic
region
that
includes
protospacer
adjacent
motif
(PAM)
just
downstream
matches
sequence.
current
challenge
in
clinical
applications
technology
is
potential
off-target
effects
cause
DNA
cleavage
at
incorrect
sites.
Off-target
genome
editing
confuses
diminishes
therapeutic
addition
potentially
casting
doubt
on
scientific
findings
regarding
activities
genes.
In
this
review,
we
summarize
recent
technological
advancements
reducing
effect
editing.
Keywords:
CRISPR-Cas9,
editing,
effect,
advancements,
review
Plants,
Год журнала:
2024,
Номер
13(14), С. 1942 - 1942
Опубликована: Июль 15, 2024
Factors
such
as
extreme
temperatures,
light
radiation,
and
nutritional
condition
influence
the
physiological,
biochemical,
molecular
processes
associated
with
fruit
development
its
quality.
Besides
abiotic
stresses,
biotic
constraints
can
also
affect
growth
Moreover,
there
be
interactions
between
stressful
conditions.
However,
it
is
challenging
to
predict
generalize
risks
of
climate
change
scenarios
on
seasonal
patterns
growth,
development,
yield,
quality
species
because
their
responses
are
often
highly
complex
involve
changes
at
multiple
levels.
Advancements
in
genetic
editing
technologies
hold
great
potential
for
agricultural
sector,
particularly
enhancing
crop
traits.
These
improvements
tailored
meet
consumer
preferences,
which
crucial
commercial
success.
Canopy
management
innovative
training
systems
key
factors
that
contribute
maximizing
yield
efficiency
improving
quality,
essential
competitiveness
orchards.
creation
habitats
support
pollinators
a
critical
aspect
sustainable
agriculture,
they
play
significant
role
production
many
crops,
including
fruits.
Incorporating
these
strategies
allows
growers
adapt
changing
conditions,
increasingly
important
stability
food
production.
By
investing
areas,
stay
ahead
challenges
opportunities
industry,
ultimately
leading
increased
success
profitability.
In
this
review,
we
aim
provide
an
updated
overview
current
knowledge
topic.
We
recommendations
future
research.
ACS Infectious Diseases,
Год журнала:
2024,
Номер
10(5), С. 1458 - 1482
Опубликована: Апрель 25, 2024
Efflux
is
a
natural
process
found
in
all
prokaryotic
and
eukaryotic
cells
that
removes
diverse
range
of
substrates
from
inside
to
outside.
Many
antibiotics
are
bacterial
efflux
pumps,
modifications
the
structure
or
overexpression
pumps
an
important
resistance
mechanism
utilized
by
many
multidrug-resistant
bacteria.
Therefore,
chemical
inhibition
revitalize
existing
has
been
considered
promising
approach
for
antimicrobial
chemotherapy
over
two
decades,
various
strategies
have
employed.
In
this
review,
we
provide
overview
multidrug
(MDR)
which
nodulation
division
(RND)
most
clinically
relevant
Gram-negative
bacteria,
describe
50
inhibitors
target
such
systems.
Although
numerous
identified
date,
none
progressed
into
clinical
use
because
formulation,
toxicity,
pharmacokinetic
issues
narrow
spectrum
inhibition.
For
these
reasons,
development
difficult
complex
area
research,
few
active
preclinical
studies
on
progress.
However,
recently
developed
tools,
including
but
not
limited
computational
tools
molecular
docking
models,
offer
hope
further
research
can
be
platform
new
inhibitors.
