Asian Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 101041 - 101041
Published: Feb. 1, 2025
Language: Английский
Trends in biotechnology, Journal Year: 2023, Volume and Issue: 41(8), P. 1000 - 1012
Published: March 30, 2023
Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR–Cas)-mediated genome editing has revolutionized biomedical research and will likely change the therapeutic diagnostic landscape. However, CRISPR–Cas9, which edits DNA by activating double-strand break (DSB) repair pathways, is not always sufficient for gene therapy applications where precise mutation required. Prime editing, latest revolution in genome-editing technologies, can achieve any possible base substitution, insertion, or deletion without requirement DSBs. prime still its infancy, further development needed to improve efficiency delivery strategies applications. We summarize developments optimization of editor (PE) variants with improved precision. Moreover, we highlight some potential
Language: Английский
Citations
92
Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10
Published: May 12, 2022
The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and its accompanying protein (Cas9) are now the most effective, efficient, precise genome editing techniques. Two essential components of CRISPR/Cas9 system guide RNA (gRNA) CRISPR-associated proteins. Choosing implementing safe effective delivery systems in therapeutic application has proven to be a significant problem. For vivo delivery, viral vectors natural specialists. Due their higher effectiveness than other methods, such as adenoviral (AdVs), adeno-associated viruses (AAVs), lentivirus (LVs) commonly employed methods. This review thoroughly examined recent achievements using variety means well benefits limitations each vector. Future thoughts for overcoming current restrictions adapting technology also discussed.
Language: Английский
Citations
83
Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(10), P. 1416 - 1416
Published: Oct. 5, 2023
In the realm of gene therapy, a pivotal moment arrived with Paul Berg’s groundbreaking identification first recombinant DNA in 1972. This achievement set stage for future breakthroughs. Conditions once considered undefeatable, like melanoma, pancreatic cancer, and host other ailments, are now being addressed at their root cause—the genetic level. Presently, therapy landscape stands adorned 22 approved vivo ex products, including IMLYGIC, LUXTURNA, Zolgensma, Spinraza, Patisiran, many more. this comprehensive exploration, we delve into rich assortment 16 drugs, from siRNA, miRNA, CRISPR/Cas9 to aptamers TRAIL/APO2L, as well 46 carriers, AAV, AdV, LNPs, exosomes naked mRNA, sonoporation, magnetofection. The article also discusses advantages disadvantages each product vector type, current challenges faced practical use its potential.
Language: Английский
Citations
55
Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14
Published: April 28, 2023
Fungal lipases (triacylglycerol acyl hydrolases EC 3.1.1.3) are significant industrial enzymes and have several applications in a number of industries fields. found species fungi yeast. These carboxylic acid esterases, categorized under the serine hydrolase family, do not require any cofactor during catalyzing reactions. It was also noticed that processes including extraction purification from comparatively easier cheaper than other sources lipases. In addition, fungal been classified into three chief classes, namely, GX, GGGX, Y. only hydrolysis fats oils (triglycerides) but involved synthetic reactions such as esterification, acidolysis, alcoholysis, interesterification, aminolysis. The production activity highly affected by carbon source, nitrogen temperature, pH, metal ions, surfactants, moisture content. Therefore, biotechnological many fields biodiesel production, ester synthesis, biodegradable biopolymers, formulations cosmetics personal care products, detergent manufacturing, degreasing leather, pulp paper textile industry, biosensor development, drug diagnostic tool medical sector, biodegradation esters, bioremediation wastewater. immobilization onto different carriers helps improving catalytic activities efficiencies increasing thermal ionic stability (in organic solvents, high temperature), being easy to recycle, inducing volume-specific loading enzyme support, thus, these features proved be appropriate for use biocatalysts sectors.
Language: Английский
Citations
52
Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 92, P. 105338 - 105338
Published: Jan. 6, 2024
The rapid advancement of CRISPR-Cas9 technology has instigated a profound transformation in genome editing with significant implications for fields like health, agriculture, and biotechnology. This abstract provides an overview the historical significance fundamental components CRISPR-Cas9, notably Cas9 protein guide RNA, underscoring its pivotal role genetic manipulation. It emphasizes CRISPR-Cas9's preeminence domain precise editing, driving breakthroughs personalized medicine, gene therapy, agriculture. Of paramount importance is integration nanomaterials, encompassing lipid-based polymeric nanoparticles, alongside viral vectors, serving as potent vehicles augmenting delivery efficiency precision. We explore strategies aimed at enhancing through while also addressing ethical regulatory considerations. In expert opinion section, we offer nuanced perspective on present state field, highlighting potential transformative progress research therapy. stands brink unlocking new possibilities providing innovative solutions to address pressing global challenges.
Language: Английский
Citations
45
Nucleic Acid Therapeutics, Journal Year: 2024, Volume and Issue: 34(2), P. 52 - 72
Published: March 20, 2024
Nucleic acid-based therapies have become the third major drug class after small molecules and antibodies. The role of nucleic has been strengthened by recent regulatory approvals tremendous clinical success. In this review, we look at obstacles that hindered field, historical milestones achieved, what is yet to be resolved anticipated soon. This review provides a view key innovations are expanding acid capabilities, setting stage for future therapeutics.
Language: Английский
Citations
35
Biologics, Journal Year: 2024, Volume and Issue: Volume 18, P. 21 - 28
Published: Jan. 1, 2024
Abstract: The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)) and the associated protein (Cas9) system, a young but well-studied genome-editing tool, holds plausible solutions to wide range of genetic disorders. single-guide RNA (sgRNA) with 20-base user-defined spacer sequence Cas9 endonuclease form core CRISPR-Cas9 system. This sgRNA can direct nuclease any genomic region that includes protospacer adjacent motif (PAM) just downstream matches sequence. current challenge in clinical applications technology is potential off-target effects cause DNA cleavage at incorrect sites. Off-target genome editing confuses diminishes therapeutic addition potentially casting doubt on scientific findings regarding activities genes. In this review, we summarize recent technological advancements reducing effect editing. Keywords: CRISPR-Cas9, editing, effect, advancements, review
Language: Английский
Citations
27
Plants, Journal Year: 2024, Volume and Issue: 13(14), P. 1942 - 1942
Published: July 15, 2024
Factors such as extreme temperatures, light radiation, and nutritional condition influence the physiological, biochemical, molecular processes associated with fruit development its quality. Besides abiotic stresses, biotic constraints can also affect growth Moreover, there be interactions between stressful conditions. However, it is challenging to predict generalize risks of climate change scenarios on seasonal patterns growth, development, yield, quality species because their responses are often highly complex involve changes at multiple levels. Advancements in genetic editing technologies hold great potential for agricultural sector, particularly enhancing crop traits. These improvements tailored meet consumer preferences, which crucial commercial success. Canopy management innovative training systems key factors that contribute maximizing yield efficiency improving quality, essential competitiveness orchards. creation habitats support pollinators a critical aspect sustainable agriculture, they play significant role production many crops, including fruits. Incorporating these strategies allows growers adapt changing conditions, increasingly important stability food production. By investing areas, stay ahead challenges opportunities industry, ultimately leading increased success profitability. In this review, we aim provide an updated overview current knowledge topic. We recommendations future research.
Language: Английский
Citations
27
ACS Infectious Diseases, Journal Year: 2024, Volume and Issue: 10(5), P. 1458 - 1482
Published: April 25, 2024
Efflux is a natural process found in all prokaryotic and eukaryotic cells that removes diverse range of substrates from inside to outside. Many antibiotics are bacterial efflux pumps, modifications the structure or overexpression pumps an important resistance mechanism utilized by many multidrug-resistant bacteria. Therefore, chemical inhibition revitalize existing has been considered promising approach for antimicrobial chemotherapy over two decades, various strategies have employed. In this review, we provide overview multidrug (MDR) which nodulation division (RND) most clinically relevant Gram-negative bacteria, describe 50 inhibitors target such systems. Although numerous identified date, none progressed into clinical use because formulation, toxicity, pharmacokinetic issues narrow spectrum inhibition. For these reasons, development difficult complex area research, few active preclinical studies on progress. However, recently developed tools, including but not limited computational tools molecular docking models, offer hope further research can be platform new inhibitors.
Language: Английский
Citations
20
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 271, P. 132696 - 132696
Published: May 31, 2024
Language: Английский
Citations
20