Water,
Год журнала:
2023,
Номер
15(19), С. 3460 - 3460
Опубликована: Сен. 30, 2023
The
COVID-19
pandemic
has
prompted
an
overwhelming
surge
in
research
investigating
different
aspects
of
the
disease
and
its
causative
agent.
In
this
study,
we
aim
to
discern
themes
trends
field
wastewater-based
epidemiology
(WBE)
SARS-CoV-2
other
respiratory
viruses
over
past
decade.
We
examined
904
papers
authored
by
researchers
from
87
countries.
Despite
low
reported
incidence
2023,
are
still
interested
application
WBE
SARS-CoV-2.
Based
on
network
visualization
mapping
189
keyword
co-occurrences,
method
optimization,
source,
transmission,
survival,
surveillance
or
early-warning
detection
systems,
variants
concern
wastewater
were
found
be
topics
greatest
interest
among
researchers.
A
trend
toward
evaluations
utility
new
technologies
such
as
digital
PCR
for
viruses,
particularly
influenza,
was
observed.
USA
emerged
leading
country
terms
publications,
citations,
international
collaborations.
Additionally,
Science
Total
Environment
stood
out
journal
with
highest
number
publications
citations.
study
highlighted
areas
further
research,
including
data
normalization
biosensor-based
collection,
emphasized
need
collaboration
standardized
methodology
future
directions.
Water Research,
Год журнала:
2024,
Номер
257, С. 121650 - 121650
Опубликована: Апрель 21, 2024
Around
the
world,
influenza
A
virus
has
caused
severe
pandemics,
and
risk
of
future
pandemics
remains
high.
Currently,
surveillance
is
based
on
clinical
diagnosis
reporting
disease
cases.
In
this
study,
we
apply
wastewater-based
to
monitor
amount
RNA
at
population
level.
We
report
levels
in
10
wastewater
treatment
plant
catchment
areas
covering
40%
Finnish
population.
Altogether,
251
monthly
composite
influent
samples
(collected
between
February
2021
2023)
were
analysed
from
supernatant
fraction
using
specific
RT-qPCR
method.
During
study
period,
an
epidemic
occurred
three
waves
Finland.
This
shows
that
can
be
detected
24
h
samples.
The
gene
copy
number
correlated
with
confirmed
cases
National
Infectious
Diseases
Register.
median
Kendall's
τ
correlation
strength
was
0.636
(min=
0.486
max=0.804)
it
statistically
significant
all
WTTPs.
Wastewater-based
independent
individual
testing
method
cost-efficiently
reflects
circulation
entire
Thus,
monitoring
complements
available,
but
often
too
sparse,
information
improves
health
care
public
preparedness
for
pandemics.
Water Research,
Год журнала:
2024,
Номер
262, С. 121989 - 121989
Опубликована: Июнь 22, 2024
Wastewater
serves
as
an
important
reservoir
of
antimicrobial
resistance
(AMR),
and
its
surveillance
can
provide
insights
into
population-level
trends
in
AMR
to
inform
public
health
policy.
This
study
compared
two
common
high-throughput
screening
approaches,
namely
(i)
quantitative
PCR
(HT
qPCR),
targeting
73
genes,
(ii)
metagenomic
sequencing.
Weekly
composite
samples
wastewater
influent
were
taken
from
47
treatment
plants
(WWTPs)
across
Wales,
part
a
national
programme,
alongside
4
weeks
daily
effluent
large
municipal
hospital.
Metagenomic
analysis
provided
more
comprehensive
resistome
coverage,
detecting
545
genes
the
targeted
by
HT
qPCR.
It
further
contextual
information
critical
risk
assessment
(i.e.
potential
bacterial
hosts).
In
contrast,
qPCR
exhibited
higher
sensitivity,
quantifying
all
including
those
clinical
relevance
present
at
low
abundance.
When
limited
target
both
methods
able
reflect
spatiotemporal
dynamics
complete
resistome,
distinguishing
that
hospital
WWTPs.
Both
approaches
revealed
correlations
between
compositional
shifts
environmental
variables
like
ammonium
concentration,
though
differed
their
interpretation
some
influencing
factors.
Overall,
metagenomics
provides
profiling,
while
permits
sensitive
quantification
significant
resistance.
We
highlight
importance
selecting
appropriate
methodologies
aligned
aims
guide
development
effective
wastewater-based
monitoring
programmes.
Journal of Environmental Exposure Assessment,
Год журнала:
2024,
Номер
3(1)
Опубликована: Фев. 26, 2024
Antimicrobial
resistance
(AMR)
is
recognized
as
one
of
the
most
serious
threats
to
public
health.
Unparalleled
population
growth
and
accelerated
rates
AMR
emergence
dissemination
have
resulted
in
both
novel
pathogenic
organisms
re-appearance
infections
that
were
formerly
under
control.
Consequently,
this
has
led
an
increased
quantity
infectious
diseases.
One
main
drivers
antimicrobial
overuse
inappropriate
prescribing
human
veterinary
medicine.
The
ability
rapidly
survey
spread
within
populations
key
for
its
prevention,
intervention,
However,
many
constraints
are
present
current
clinical
surveillance
systems
their
capacity
determine
dynamics
microbiome
healthy
individuals
well
pathogens
causing
infections.
Wastewater-based
epidemiology
(WBE)
emergent
technique
act
a
supplementary
measure
disease
early
warning
system
outbreaks.
development
outbreaks
community
level
can
be
monitored
real
time
through
analysis
pooled
wastewater.
This
review
provides
introduction
using
wastewater-based
monitor
bacteria,
overview
components.
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 281 - 281
Опубликована: Янв. 26, 2025
Wastewater
surveillance
is
a
powerful
public
health
tool
which
gained
global
prominence
during
the
COVID-19
pandemic.
This
article
describes
development
and
implementation
of
national
wastewater
network
in
France:
SUM’EAU.
Preliminary
work
included
defining
sampling
strategy,
evaluating/optimising
analytical
methods,
launching
call
for
tenders
to
select
laboratories
producing
monitoring
indicators.
SUM’EAU
was
then
deployed
three
stages:
(i)
pilot
study,
(ii)
transfer
activities
from
National
Reference
Laboratory
four
selected
laboratories,
(iii)
extension
system
additional
sites.
Currently,
monitors
SARS-CoV-2
across
54
treatment
plants
mainland
France.
Once
week
on
business
days,
24
h
flow-proportional
composite
samples
are
collected
at
plant
inlets
transported
5
°C
(±3
°C)
partner
analysis.
The
process
involves
sample
concentration,
RNA
extraction,
digital
RT-PCR/q-RT-PCR
detect
quantify
presence
genome
wastewater.
Subsequently,
data
transferred
Santé
publique
France,
French
Public
Health
Agency,
analysis
interpretation.
While
has
been
instrumental
pandemic
holds
significant
potential
broader
application,
securing
sustainable
funding
its
operation
remains
major
challenge.
International Journal of Environmental Research and Public Health,
Год журнала:
2025,
Номер
22(1), С. 97 - 97
Опубликована: Янв. 12, 2025
Antimicrobial
resistance
(AMR)
is
a
growing
global
health
threat.
This
study
investigated
antibiotic
in
E.
coli
isolates
from
municipal
wastewater
(86
isolates)
and
clinical
urinary
tract
infection
(UTI)
cases
(34
Grenadian
community,
using
data
January
2022
to
October
2023.
Antibiogram
data,
assessed
per
WHO
guidelines
for
Critically
Important
antimicrobials
(CIA),
showed
the
highest
levels
both
samples
ampicillin,
followed
by
amoxicillin/clavulanic
acid
nalidixic
acid,
all
classified
as
Important.
Similar
was
observed
sulfamethoxazole-trimethoprim
(highly
important)
groups,
with
nitrofurantoin
showing
important
category.
According
AWaRe
classification,
ampicillin
(ACCESS
group)
had
resistance,
while
lowest
across
samples.
The
WATCH
group
antibiotics,
cefuroxime
cefoxitin,
comparable
levels,
whereas
aztreonam
RESERVE
(tested
only
wastewater)
100%
sensitive.
Multiple
Antibiotic
Resistance
(MAR)
index
analysis
revealed
that
7%
of
38.2%
MAR
values
over
0.2,
indicating
prior
exposure
isolates.
These
parallel
patterns
highlight
monitoring
valuable
tool
AMR
surveillance,
supporting
stewardship
through
ongoing
environmental
assessment.
