SARS-CoV-2 an extensive monitoring of an Italian full-scale wastewater treatment plant DOI Creative Commons
Alida Cosenza, Carmelo Massimo Maida,

Marta Vullo

et al.

Journal of Water Process Engineering, Journal Year: 2024, Volume and Issue: 63, P. 105469 - 105469

Published: May 14, 2024

Wastewater-based epidemiology was adopted to monitor SARS-CoV-2 RNA in Caltanissetta (Sicily, Italy) full-scale wastewater treatment plant (WWTP). The sampling campaign lasted 288 days (from October 11, 2021 July 26, 2022). Influent wastewater, effluent from the secondary clarifier, and disinfected samples were monitored for coupled with other conventional pollutants (total suspended solids – TSS, chemical oxygen demand COD, biochemical BOD, respectively, Escherichia coli). Results showed that performs excellently removing (average removal of 94 %, 91 % respectively). detected all influent analyzed 1.1 × 105 copies genomic per litre GC L−1). Within biological process, a strong degradation detected. High correlation between log-transformed active cases obtained (correlation coefficient 0.85, p-value < 0.001when 14 lag time considered).

Language: Английский

Wastewater-based surveillance is an efficient monitoring tool for tracking influenza A in the community DOI Creative Commons
K. Lehto, Annika Länsivaara, Rafiqul Hyder

et al.

Water Research, Journal Year: 2024, Volume and Issue: 257, P. 121650 - 121650

Published: April 21, 2024

Around the world, influenza A virus has caused severe pandemics, and risk of future pandemics remains high. Currently, surveillance is based on clinical diagnosis reporting disease cases. In this study, we apply wastewater-based to monitor amount RNA at population level. We report levels in 10 wastewater treatment plant catchment areas covering 40% Finnish population. Altogether, 251 monthly composite influent samples (collected between February 2021 2023) were analysed from supernatant fraction using specific RT-qPCR method. During study period, an epidemic occurred three waves Finland. This shows that can be detected 24 h samples. The gene copy number correlated with confirmed cases National Infectious Diseases Register. median Kendall's τ correlation strength was 0.636 (min= 0.486 max=0.804) it statistically significant all WTTPs. Wastewater-based independent individual testing method cost-efficiently reflects circulation entire Thus, monitoring complements available, but often too sparse, information improves health care public preparedness for pandemics.

Language: Английский

Citations

20
National-scale antimicrobial resistance surveillance in wastewater: A comparative analysis of HT qPCR and metagenomic approaches DOI Creative Commons
Margaret E. Knight, Gordon Webster, William Bernard Perry

et al.

Water Research, Journal Year: 2024, Volume and Issue: 262, P. 121989 - 121989

Published: June 22, 2024

Wastewater serves as an important reservoir of antimicrobial resistance (AMR), and its surveillance can provide insights into population-level trends in AMR to inform public health policy. This study compared two common high-throughput screening approaches, namely (i) quantitative PCR (HT qPCR), targeting 73 genes, (ii) metagenomic sequencing. Weekly composite samples wastewater influent were taken from 47 treatment plants (WWTPs) across Wales, part a national programme, alongside 4 weeks daily effluent large municipal hospital. Metagenomic analysis provided more comprehensive resistome coverage, detecting 545 genes the targeted by HT qPCR. It further contextual information critical risk assessment (i.e. potential bacterial hosts). In contrast, qPCR exhibited higher sensitivity, quantifying all including those clinical relevance present at low abundance. When limited target both methods able reflect spatiotemporal dynamics complete resistome, distinguishing that hospital WWTPs. Both approaches revealed correlations between compositional shifts environmental variables like ammonium concentration, though differed their interpretation some influencing factors. Overall, metagenomics provides profiling, while permits sensitive quantification significant resistance. We highlight importance selecting appropriate methodologies aligned aims guide development effective wastewater-based monitoring programmes.

Language: Английский

Citations

9
Wastewater-Based Epidemiological Surveillance in France: The SUM’EAU Network DOI Creative Commons
Frédéric Jourdain, L. Toro,

Senta-Loÿs Zoé

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 281 - 281

Published: Jan. 26, 2025

Wastewater surveillance is a powerful public health tool which gained global prominence during the COVID-19 pandemic. This article describes development and implementation of national wastewater network in France: SUM’EAU. Preliminary work included defining sampling strategy, evaluating/optimising analytical methods, launching call for tenders to select laboratories producing monitoring indicators. SUM’EAU was then deployed three stages: (i) pilot study, (ii) transfer activities from National Reference Laboratory four selected laboratories, (iii) extension system additional sites. Currently, monitors SARS-CoV-2 across 54 treatment plants mainland France. Once week on business days, 24 h flow-proportional composite samples are collected at plant inlets transported 5 °C (±3 °C) partner analysis. The process involves sample concentration, RNA extraction, digital RT-PCR/q-RT-PCR detect quantify presence genome wastewater. Subsequently, data transferred Santé publique France, French Public Health Agency, analysis interpretation. While has been instrumental pandemic holds significant potential broader application, securing sustainable funding its operation remains major challenge.

Language: Английский

Citations

1
A review of wastewater-based epidemiology for antimicrobial resistance surveillance DOI Open Access

Leah Clarke,

Jake O’Brien, Aimee K. Murray

et al.

Journal of Environmental Exposure Assessment, Journal Year: 2024, Volume and Issue: 3(1)

Published: Feb. 26, 2024

Antimicrobial resistance (AMR) is recognized as one of the most serious threats to public health. Unparalleled population growth and accelerated rates AMR emergence dissemination have resulted in both novel pathogenic organisms re-appearance infections that were formerly under control. Consequently, this has led an increased quantity infectious diseases. One main drivers antimicrobial overuse inappropriate prescribing human veterinary medicine. The ability rapidly survey spread within populations key for its prevention, intervention, However, many constraints are present current clinical surveillance systems their capacity determine dynamics microbiome healthy individuals well pathogens causing infections. Wastewater-based epidemiology (WBE) emergent technique act a supplementary measure disease early warning system outbreaks. development outbreaks community level can be monitored real time through analysis pooled wastewater. This review provides introduction using wastewater-based monitor bacteria, overview components.

Language: Английский

Citations

8
Combating antibiotic resistance using wastewater surveillance: Significance, applications, challenges, and future directions DOI
Sahar Gholipour, Zahra Shamsizadeh, Dariusz Halabowski

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 168056 - 168056

Published: Oct. 31, 2023

Language: Английский

Citations

16
The role of wastewater-based epidemiology for SARS-CoV-2 in developing countries: Cumulative evidence from South Africa supports sentinel site surveillance to guide public health decision-making DOI
Chinwe Juliana Iwu, Nkosenhle Ndlovu, R. Saïd

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 903, P. 165817 - 165817

Published: July 26, 2023

Language: Английский

Citations

13
Nursing home wastewater surveillance for early warning of SARS-CoV-2-positive occupants—Insights from a pilot project at 8 facilities DOI
Shantini D. Gamage, Chetan Jinadatha, Vincent Rizzo

et al.

American Journal of Infection Control, Journal Year: 2024, Volume and Issue: 52(6), P. 701 - 706

Published: Jan. 4, 2024

Language: Английский

Citations

4
Phenotypic Antibiotic Resistance Patterns of Escherichia coli Isolates from Clinical UTI Samples and Municipal Wastewater in a Grenadian Community DOI Open Access

M Matthew-Bernard,

K Farmer-Diaz,

Grace Dolphin-Bond

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2025, Volume and Issue: 22(1), P. 97 - 97

Published: Jan. 12, 2025

Antimicrobial resistance (AMR) is a growing global health threat. This study investigated antibiotic in E. coli isolates from municipal wastewater (86 isolates) and clinical urinary tract infection (UTI) cases (34 Grenadian community, using data January 2022 to October 2023. Antibiogram data, assessed per WHO guidelines for Critically Important antimicrobials (CIA), showed the highest levels both samples ampicillin, followed by amoxicillin/clavulanic acid nalidixic acid, all classified as Important. Similar was observed sulfamethoxazole-trimethoprim (highly important) groups, with nitrofurantoin showing important category. According AWaRe classification, ampicillin (ACCESS group) had resistance, while lowest across samples. The WATCH group antibiotics, cefuroxime cefoxitin, comparable levels, whereas aztreonam RESERVE (tested only wastewater) 100% sensitive. Multiple Antibiotic Resistance (MAR) index analysis revealed that 7% of 38.2% MAR values over 0.2, indicating prior exposure isolates. These parallel patterns highlight monitoring valuable tool AMR surveillance, supporting stewardship through ongoing environmental assessment.

Language: Английский

Citations

0
The Crucial Role of Laboratory Medicine in Addressing Future Public Health Infectious Threats: Insights Gained from the COVID-19 Pandemic DOI Creative Commons
Giuseppe Lippi, Brandon Michael Henry, Camilla Mattiuzzi

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(3), P. 323 - 323

Published: Jan. 30, 2025

Laboratory testing has played a pivotal role throughout the coronavirus disease 2019 (COVID-19) pandemic, exemplifying importance of in vitro diagnostics addressing public health threats posed by outbreaks infectious diseases. This article aims to present key insights from our expertise, derived evidence gathered during COVID-19 inform strategies for managing future challenges. Current scientific underscores that patient sample not only allows diagnose an acute severe respiratory syndrome 2 (SARS-CoV-2) infection, but also supports outbreak prediction, improved control measures, anticipation pressure on healthcare system, mitigation adverse clinical outcomes, and early detection emerging SARS-CoV-2 variants. Additionally, wastewater monitoring emerged as powerful tool forecasting burden, including both prevalence severity. Collectively, these findings underscore value diagnostic surveillance guiding planning optimizing resource allocation offering valid framework be applied threats, especially any potential “Disease X” may emerge future.

Language: Английский

Citations

0
Monitoring Antibiotic Resistance in Wastewater: Findings from Three Treatment Plants in Sicily, Italy DOI Open Access
Roberta Magnano San Lio, Andrea Maugeri, Martina Barchitta

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2025, Volume and Issue: 22(3), P. 351 - 351

Published: Feb. 27, 2025

Antimicrobial resistance (AMR) poses a global public health threat. Wastewater analysis provides valuable insights into antimicrobial genes (ARGs), identifying sources and trends evaluating AMR control measures. Between February 2022 March 2023, pre-treatment urban wastewater samples were collected weekly from treatment plants in Pantano D'Arci, Siracusa, Giarre (Sicily, Italy). Monthly composite DNA extracts prepared by combining subsamples each site, yielding 42 samples-14 plant. Real-time PCR targeted specific ARGs, including blaSHV, erm(A), erm(B), blaOXA, blaNDM, blaVIM, blaTEM, blaCTX-M. The preliminary findings revealed that blaERM-B, blaCTX-M present all samples, with erm(B) (median value: 8.51; range: 1.67-30.93), blaSHV (0.78; 0.00-6.36), blaTEM (0.72; 0.34-4.30) showing the highest relative abundance. These results underscore importance of integrating ARG data broader research to understand persistence proliferation mechanisms ARGs environments. Future studies should employ metagenomic analyses profile resistomes urban, hospital, agricultural, farm wastewater. Comparing these profiles will help identify contamination pathways inform development surveillance programs. Monitoring shifts abundance could signal cross-sectoral contamination, enabling more effective strategies.

Language: Английский

Citations

0