Biological Functions of Macromolecular Protein Hydrogels in Constructing Osteogenic Microenvironment DOI
Yihan Wang, Huixin Lv, Sicong Ren

и другие.

ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(9), С. 5513 - 5536

Опубликована: Авг. 22, 2024

Irreversible bone defects resulting from trauma, infection, and degenerative illnesses have emerged as a significant health concern. Structurally functionally controllable hydrogels made by tissue engineering (BTE) become promising biomaterials. Natural proteins are able to establish connections with autologous through unique biologically active regions. Hydrogels based on can simulate the microenvironment regulate biological behavior of stem cells in niche, making them candidates for research related regeneration. This article reviews functions various natural macromolecular (such collagen, gelatin, fibrin, silk fibroin) highlights their special advantages hydrogels. Then latest trends cross-linking modified protein improved mechanical properties composite loaded exogenous micromolecular been discussed. Finally, applications hydrogels, such 3D printed microspheres, injectable were introduced, aiming provide reference repair clinical defects.

Язык: Английский

New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay DOI Creative Commons
Zhengpeng Wan, Marie Floryan, Mark F. Coughlin

и другие.

Advanced Healthcare Materials, Год журнала: 2022, Номер 12(14)

Опубликована: Ноя. 5, 2022

Abstract Previous studies have developed vascularized tumor spheroid models to demonstrate the impact of intravascular flow on progression and treatment. However, these not been widely adopted so vascularization spheroids in vitro is generally lower than tissues vivo. To improve level, a new strategy introduced form by adding fibroblasts (FBs) sequentially pre‐formed this method with cell lines from kidney, lung, ovary cancer. Tumor made higher FB densities periphery spheroid, which tend enhance vascularization. The vessels close are more perfusable ones other methods. Finally, chimeric antigen receptor (CAR) T cells perfused under continuous into immunotherapy evaluation using tumor‐on‐a‐chip model. This for establishing leads increased vitro, allowing examination immune, endothelial, stromal, responses static or conditions.

Язык: Английский

Процитировано

47
Advances in the Model Structure of In Vitro Vascularized Organ-on-a-Chip DOI Creative Commons

Hongze Yin,

Yue Wang, Na Liu

и другие.

Cyborg and Bionic Systems, Год журнала: 2024, Номер 5

Опубликована: Янв. 1, 2024

Microvasculature plays a crucial role in human physiology and is closely related to various diseases. Building vitro vascular networks essential for studying tissue behavior with repeatable morphology signaling conditions. Engineered 3D microvascular network models, developed through advanced microfluidic-based techniques, provide accurate reproducible platforms the microvasculature vitro, an component designing organ-on-chips achieve greater biological relevance. By optimizing microstructure of microfluidic devices mimic vivo microenvironment, organ-specific models healthy pathological tissues can be created. This review summarizes recent advancements strategies constructing devices. It discusses static vascularization chips’ classification, structural characteristics, techniques used build them: growing blood vessels on chips either or dynamic, grown microchannels, elastic membranes, hydrogels. Finally, paper application scenarios key technical issues existing chips. also explores potential novel organoid chip approach that combines organoids organ generate better

Язык: Английский

Процитировано

9
Emerging strategies of engineering retinal organoids and organoid-on-a-chip in modeling intraocular drug delivery: Current progress and future perspectives DOI
Jiaheng Yu, Yuqi Yin,

Yubing Leng

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 197, С. 114842 - 114842

Опубликована: Апрель 25, 2023

Язык: Английский

Процитировано

16
Emerging technologies for quality control of cell-based, advanced therapy medicinal products DOI Creative Commons

Silvia Zia,

Valeria Pizzuti, Francesca Paris

и другие.

Journal of Pharmaceutical and Biomedical Analysis, Год журнала: 2024, Номер 246, С. 116182 - 116182

Опубликована: Апрель 29, 2024

Advanced therapy medicinal products (ATMP) are complex medicines based on gene therapy, somatic cell and tissue engineering. These rapidly arising as novel promising therapies for a wide range of different clinical applications. The process the development well-established ATMPs is challenging. Many issues must be considered from raw material, manufacturing, safety, pricing to assure quality their implementation innovative therapeutic tools. Among ATMPs, cell-based drugs altogether. As standard drugs, technologies control, non-invasive isolation production then needed ensure expanding applications ameliorate safety standardization production. In this review, emerging approaches control described. new techniques, microfluid-based systems show advantages related miniaturization, easy in analytical automation which allow final product.

Язык: Английский

Процитировано

6
Gellan gum-gelatin based cardiac models support formation of cellular networks and functional cardiomyocytes DOI Creative Commons
Hanna Vuorenpää, Joona Valtonen, Kirsi Penttinen

и другие.

Cytotechnology, Год журнала: 2024, Номер 76(4), С. 483 - 502

Опубликована: Май 2, 2024

Abstract Cardiovascular diseases remain as the most common cause of death worldwide. To reveal underlying mechanisms in varying cardiovascular diseases, vitro models with cells and supportive biomaterial can be designed to recapitulate essential components human heart. In this study, we analyzed whether 3D co-culture cardiomyocytes (CM) vascular network adipose tissue-derived mesenchymal stem/stromal (ASC) support CM functionality. were cultured either endothelial (EC) ASC or only hydrazide-modified gelatin oxidized gellan gum hybrid hydrogel form multiculture myocardial co-culture, respectively. We studied functional characteristics two different cellular set-ups formation, morphology orientation. The results showed that gum-gelatin supports formation networks CM. detected a modest extensive ASC-derived alpha smooth muscle actin -positive multi- co-culture. iPSC-CM elongated morphology, partly aligned orientation formed presented normal calcium transients, beating rates, contraction relaxation behavior both setups. These cardiac provide promising platforms study (patho) physiological diseases.

Язык: Английский

Процитировано

5
Advancing bone regeneration: Unveiling the potential of 3D cell models in the evaluation of bone regenerative materials DOI
Minglu Hao,

Linyuan Xue,

Xiaobo Wen

и другие.

Acta Biomaterialia, Год журнала: 2024, Номер 183, С. 1 - 29

Опубликована: Май 28, 2024

Язык: Английский

Процитировано

5
Differentiation of mesenchymal stem cells into vascular endothelial cells in 3D culture: a mini review DOI

Elrade Rofaani,

Mohammad Mardani,

P. N. Yutiana

и другие.

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Июнь 24, 2024

Язык: Английский

Процитировано

5
Highly sensitive microfluidic sensor using integrated optical fiber and real-time single-cell Raman spectroscopy for diagnosis of pancreatic cancer DOI Creative Commons
Renhao Ni,

Kaixin Ge,

Yang Luo

и другие.

Biosensors and Bioelectronics, Год журнала: 2024, Номер 264, С. 116616 - 116616

Опубликована: Авг. 3, 2024

Pancreatic cancer is notoriously lethal due to its late diagnosis and poor patient response treatments, posing a significant clinical challenge. This study introduced novel approach that combines single-cell capturing platform, tumor-targeted silver (Ag) nanoprobes, precisely docking tapered fiber integrated with Raman spectroscopy. focuses on early detection progression monitoring of pancreatic cancer. Utilizing Ag nanoparticles multimode fibers enhances signals, minimizes light loss, reduces background noise. advanced system allows for detailed molecular spectroscopic examination individual cells, offering more practical information enabling earlier accurate staging compared conventional multicellular Transcriptomic analysis using high-throughput gene screening transcriptomic databases confirmed the ability accuracy this method identify changes in normal, early, metastatic cells. Key findings revealed cell adhesion, migration, extracellular matrix are closely related spectroscopy (SCRS) results, highlighting components such as collagen, phospholipids, carotene. Therefore, SCRS provides comprehensive view composition, biological function, material novel, accurate, reliable, rapid, efficient diagnosing

Язык: Английский

Процитировано

4
Effects of Magnesium-Doped Hydroxyapatite Nanoparticles on Bioink Formulation for Bone Tissue Engineering DOI Creative Commons
Margherita Montanari, Jannika T. Korkeamäki, Elisabetta Campodoni

и другие.

ACS Applied Bio Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Bioprinting of nanohydroxyapatite (nHA)-based bioinks has attracted considerable interest in bone tissue engineering. However, the role and relevance physicochemical properties nHA incorporated a bioink, particularly terms its printability biological behavior bioprinted cells, remain largely unexplored. In this study, two bioinspired nHAs with different chemical compositions, crystallinity, morphologies were synthesized characterized: more crystalline, needle-like Mg2+-doped (N-HA) amorphous, rounded Mg2+- CO32–-doped (R-HA). To investigate effects compositions these nanoparticles on bioprinting human marrow stromal cells (hBMSCs), gelatin methacryloyl (GelMA) selected as bioink backbone. The addition 1% (w/w) bioceramic significantly improved GelMA extrudability, buildability, filament spreading. potential was evaluated by examining hBMSC viability, metabolic activity, osteogenic differentiation over 21 days. Both showed high cell N-HA showing significant increase activity under nonosteogenic conditions R-HA notable stimulation. These results suggest that interact differently their environment, highlighting importance both chemistry morphology performance. addition, further highlighted how influence markers at RNA protein levels. Clearly, tailoring hydroxyapatite is critical to developing biomimetic great for advancing applications.

Язык: Английский

Процитировано

0
Treatment Response to Oncolytic Virus in Patient-Derived Breast Cancer and Hypopharyngeal Cancer Organoids: Evaluation via a Microfluidics Organ-on-a-Chip System DOI Creative Commons
Yu Sun,

Jiaqi Liu,

Li Zhu

и другие.

Bioengineering, Год журнала: 2025, Номер 12(2), С. 146 - 146

Опубликована: Фев. 4, 2025

In this study, we present an oncolytic virus (OV) evaluation system established using microfluidic organ-on-a-chip (OOC) systems and patient-derived organoids (PDOs), which was used in the development of a novel virus, AD4-GHPE. An OV offers advantages such as good targeting ability minimal side effects, it has achieved significant breakthroughs when combined with immunotherapy recent clinical trials. The OVs become emerging research focus. PDOs can preserve heterogeneity situ tumor tissues, whereas OOC automate standardize various experimental procedures. These have been applied cutting-edge drug screening cell therapy experiments; however, their use functionally complex viruses remains to be explored. constructed recombinant adenovirus, AD4-GHPE, evaluated through functional validations hypopharyngeal breast cancer organoids. results confirmed that AD4-GHPE exhibits three antitumor mechanisms, namely, tumor-specific cytotoxicity, reduction programmed death ligand 1 (PD-L1) expression cells increase CD8+ T-cell activity, granulocyte–macrophage colony-stimulating factor (GM-CSF) secretion. combining efficient reliable, providing personalized treatment recommendations for patients offering industrialized standardized ideas OVs.

Язык: Английский

Процитировано

0