3D printed Aloe barbadensis loaded alginate-gelatin hydrogel for wound healing and scar reduction: In vitro and in vivo study

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139745 - 139745

Опубликована: Янв. 1, 2025

Язык: Английский

---

Development of an innovative cylindrical carbon nanofiber/gelatin-polycaprolactone hydrogel scaffold for enhanced bone regeneration

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141250 - 141250

Опубликована: Фев. 1, 2025

Язык: Английский

---

Leveraging the nanotopography of filamentous fungal chitin-glucan nano/microfibrous spheres (FNS) coated with collagen (type I) for scaffolded fibroblast spheroids in regenerative medicine

Tissue and Cell, Год журнала: 2025, Номер 93, С. 102734 - 102734

Опубликована: Янв. 10, 2025

Язык: Английский

---

Development of Recombinant Human Collagen-Based Porous Scaffolds for Skin Tissue Engineering: Enhanced Mechanical Strength and Biocompatibility

Polymers, Год журнала: 2025, Номер 17(3), С. 303 - 303

Опубликована: Янв. 23, 2025

Skin tissue engineering scaffolds should possess key properties such as porosity, degradability, durability, and biocompatibility to effectively facilitate skin cell adhesion growth. In this study, recombinant human collagen (RHC) was used fabricate porous via freeze-drying, offering an alternative animal-derived where bovine (BC)-based were also prepared for comparison. The internal morphology of the RHC characterized by scanning electron microscopy (SEM) pore size ranged from 68.39 117.52 µm. results compression fatigue tests showed that mechanical strength durability could be tailored adjusting concentration, maximum compressive modulus reached 0.003 MPa, which is comparable BC scaffolds. degradation test illustrated had a slower rate compared Finally, biocompatibilities studied seeding culturing foreskin fibroblasts (HFFs) umbilical vein endothelial cells (HUVECs) in samples. fluorescent images Cell Counting Kit-8 (CCK-8) assay revealed non-cytotoxic supported attachment well proliferation seeded cells. Overall, demonstrated RHC-based exhibited adequate strength, ideal biodegradability, exceptional biocompatibility, making them highly suitable skin-tissue-engineering applications.

Язык: Английский

---

Biomimetic chitosan-based scaffold for 3D breast cancer cell culture: a promising tool for anticancer drug screening

Macromolecular Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

Язык: Английский

---

Three-Dimensional Printed Biomimetic Elastomeric Scaffolds: Experimental Study of Surface Roughness and Pore Generation

Biomimetics, Год журнала: 2025, Номер 10(2), С. 95 - 95

Опубликована: Фев. 8, 2025

Tissue engineering is an emerging field within biomedicine, related to developing functional substitutes for damaged tissues or organs. Despite significant advancements, the development of effective tissue constructs remains challenging, particularly when replicating elastic stretchability, which plays a critical role in many tissues. Therefore, tough, elastomeric scaffolds that mimic complex elasticity native tissues, such as myocardium, heart valves, and blood vessels, particular interest. This study aims evaluate flexible printable material (Formlabs' Elastic 50A Resin V2) develop porous 3D using additive manufacturing stereolithography (SLA). The samples were tested relation their swelling behaviour, mechanical properties, exposure low temperatures. Additionally, effects print orientation, water immersion, temperatures on surface roughness porosity investigated determine best conditions enhance scaffold performance biomedical applications. results demonstrated printed at 0°, immersed water, exposed temperature (-80 °C) showed more uniform microporosity, could improve adhesion growth cells scaffold. research highlights practical economical approach enhancing scaffolds, paving way improved outcomes

Язык: Английский

---

Inducing Osteogenesis in Human Pulp Stem Cells Cultured on Nano-Hydroxyapatite and Naringin-Coated 3D-Printed Poly Lactic Acid Scaffolds

Polymers, Год журнала: 2025, Номер 17(5), С. 596 - 596

Опубликована: Фев. 24, 2025

Regeneration dentistry demonstrates significant challenges due to the complexity of different dental structures. This study aimed investigate osteogenic differentiation human pulp stem cells (hDPSCs) cultured on a 3D-printed poly lactic acid (PLA) scaffold coated with nano-hydroxyapatite (nHA) and naringin (NAR) as model for regenerative. PLA scaffolds were 3D printed into circular discs (10 × 1 mm) nHA, NAR, or both. Scaffolds hDPTCs identify cellular morphological changes adhesion over incubation periods 3, 7, 21 days using SEM. Then, potential PLA, PLA/nHA/NAR, MTA elutes (PLA/MTA scaffolds) evaluated by measuring mineralized tissue deposition calcium concentration assays alizarin red staining (ARS). Also, immunofluorescence labelling alkaline phosphatase (ALP) dentine sialophosphoprotein (DSPP) within evaluated. The highest attachment was identified PLA/nHA/NAR scaffold, reflecting differentiation. ARS recognized in culture, statistically difference (p < 0.05) compared PLA/MTA. ALP DSPP markers showed significantly higher immunoreactivity results confirm nHA/NAR future animal investigations.

Язык: Английский

---

Investigation of Silicon Oxide Nanoparticle-Enhanced Self-Healing Hydrogel for Cartilage Repair and Regeneration in Rabbit Earlobe Models

Journal of drug targeting, Год журнала: 2025, Номер unknown, С. 1 - 24

Опубликована: Фев. 28, 2025

This study developed an alginate, gelatin, and chondroitin sulfate hydrogel incorporating silicon oxide nanoparticles to assess morphology, cell proliferation, viability. The effectiveness of these hydrogels for cartilage repair was evaluated in vivo using male albino rabbits, divided into three groups: a control group without hydrogels, observer with lacking nanoparticles, treatment nanoparticle-enhanced post-injury repair. At 15, 30, 60 days post-surgery, the rabbits were humanely euthanized, excised tissue samples fixed 10% formalin histopathological analysis, then processed embedded paraffin microscopic evaluation. Statistical analysis performed SPSS software ANOVA Tukey's post hoc test. Results indicated that supported viability encouraged differentiation chondrocyte-like phenotypes. Scanning Electron Microscopy (SEM) confirmed hydrogels' porosity showed significant differences survival rates compared group, underscoring potential engineering regenerative strategies.

Язык: Английский

---

Early postoperative conjunctival complications leading to exposure of surgically implanted CorNeat EverPatch devices

Ophthalmology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

To compare the early exposure and surgical revision rates between a new synthetic tissue substitute (CorNeat EverPatch) with that of human donor cornea following placement onto scleral surface during ophthalmic surgery, to study biomaterial properties patch material. Retrospective comparison study; analyses explanted All consecutive patients who underwent surgery implantation CorNeat EverPatch at Wilmer Eye Institute (occurring from February August 2024) group irradiated cornea, matched 1:2 receiving for age, type glaucoma, surgeon. review clinical electronic medical records Institute. Materials characterization including morphology, roughness, wettability, thermal stability, elemental analysis, physical properties. Early (within 5 months surgery) or surgery. Thirty undergoing in 2024 received primary tube shunt (N=27), (N=2), covering exposed suture used fixation an intraocular lens (N=1). During postoperative period, rate was 48.3% 27.9%. In case-matched control (N=58), graft 1.7% (p<0.0001) (p<0.0001). devices were comprised randomly aligned fibrous mesh average fiber diameter 1.36 ± 0.78 μm, roughness 1.3 0.1 pore size 3.7 0.4 μm2, percent porosity 37 3%. Explanted demonstrated varying degrees integration significantly increased wettability changes stability composition. The conjunctival complications leading higher than cases involving corneal grafts.

Язык: Английский

---

Biopolymeric Scaffolds with Melatonin for Tissue Engineering—A Review

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2520 - 2520

Опубликована: Март 11, 2025

Melatonin, a natural hormone with antioxidant, anti-inflammatory, and regenerative properties, has gained increasing attention in tissue engineering for its ability to enhance the therapeutic potential of biopolymeric scaffolds. These scaffolds, designed mimic extracellular matrix, provide structural support bioactive environment regeneration. By integrating melatonin, researchers aim create multifunctional scaffolds that promote cell proliferation, modulate inflammatory responses, improve wound healing outcomes. Challenges utilizing melatonin include maintaining stability under light, heat, oxygen exposure, optimizing release profile sustained effects. Innovative fabrication methods, such as electrospinning, 3D printing, lyophilization, have enabled precise control over scaffold architecture delivery. techniques ensure enhanced interactions target tissues tailored regeneration processes. Combining growth factors, cytokines, antimicrobial agents offers applications, from chronic management bone nerve Continued research this field promises transformative solutions medicine, expanding clinical applicability melatonin-enriched This review highlights current progress, challenges, opportunities associated harnessing melatonin’s within frameworks.

Язык: Английский

---