
Polymer Testing, Год журнала: 2025, Номер unknown, С. 108875 - 108875
Опубликована: Май 1, 2025
Язык: Английский
Polymer Testing, Год журнала: 2025, Номер unknown, С. 108875 - 108875
Опубликована: Май 1, 2025
Язык: Английский
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139745 - 139745
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
2International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141250 - 141250
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
2Tissue and Cell, Год журнала: 2025, Номер 93, С. 102734 - 102734
Опубликована: Янв. 10, 2025
Язык: Английский
Процитировано
1Polymers, Год журнала: 2025, Номер 17(3), С. 303 - 303
Опубликована: Янв. 23, 2025
Skin tissue engineering scaffolds should possess key properties such as porosity, degradability, durability, and biocompatibility to effectively facilitate skin cell adhesion growth. In this study, recombinant human collagen (RHC) was used fabricate porous via freeze-drying, offering an alternative animal-derived where bovine (BC)-based were also prepared for comparison. The internal morphology of the RHC characterized by scanning electron microscopy (SEM) pore size ranged from 68.39 117.52 µm. results compression fatigue tests showed that mechanical strength durability could be tailored adjusting concentration, maximum compressive modulus reached 0.003 MPa, which is comparable BC scaffolds. degradation test illustrated had a slower rate compared Finally, biocompatibilities studied seeding culturing foreskin fibroblasts (HFFs) umbilical vein endothelial cells (HUVECs) in samples. fluorescent images Cell Counting Kit-8 (CCK-8) assay revealed non-cytotoxic supported attachment well proliferation seeded cells. Overall, demonstrated RHC-based exhibited adequate strength, ideal biodegradability, exceptional biocompatibility, making them highly suitable skin-tissue-engineering applications.
Язык: Английский
Процитировано
1Macromolecular Research, Год журнала: 2025, Номер unknown
Опубликована: Апрель 11, 2025
Язык: Английский
Процитировано
1Biomimetics, Год журнала: 2025, Номер 10(2), С. 95 - 95
Опубликована: Фев. 8, 2025
Tissue engineering is an emerging field within biomedicine, related to developing functional substitutes for damaged tissues or organs. Despite significant advancements, the development of effective tissue constructs remains challenging, particularly when replicating elastic stretchability, which plays a critical role in many tissues. Therefore, tough, elastomeric scaffolds that mimic complex elasticity native tissues, such as myocardium, heart valves, and blood vessels, particular interest. This study aims evaluate flexible printable material (Formlabs' Elastic 50A Resin V2) develop porous 3D using additive manufacturing stereolithography (SLA). The samples were tested relation their swelling behaviour, mechanical properties, exposure low temperatures. Additionally, effects print orientation, water immersion, temperatures on surface roughness porosity investigated determine best conditions enhance scaffold performance biomedical applications. results demonstrated printed at 0°, immersed water, exposed temperature (-80 °C) showed more uniform microporosity, could improve adhesion growth cells scaffold. research highlights practical economical approach enhancing scaffolds, paving way improved outcomes
Язык: Английский
Процитировано
0Polymers, Год журнала: 2025, Номер 17(5), С. 596 - 596
Опубликована: Фев. 24, 2025
Regeneration dentistry demonstrates significant challenges due to the complexity of different dental structures. This study aimed investigate osteogenic differentiation human pulp stem cells (hDPSCs) cultured on a 3D-printed poly lactic acid (PLA) scaffold coated with nano-hydroxyapatite (nHA) and naringin (NAR) as model for regenerative. PLA scaffolds were 3D printed into circular discs (10 × 1 mm) nHA, NAR, or both. Scaffolds hDPTCs identify cellular morphological changes adhesion over incubation periods 3, 7, 21 days using SEM. Then, potential PLA, PLA/nHA/NAR, MTA elutes (PLA/MTA scaffolds) evaluated by measuring mineralized tissue deposition calcium concentration assays alizarin red staining (ARS). Also, immunofluorescence labelling alkaline phosphatase (ALP) dentine sialophosphoprotein (DSPP) within evaluated. The highest attachment was identified PLA/nHA/NAR scaffold, reflecting differentiation. ARS recognized in culture, statistically difference (p < 0.05) compared PLA/MTA. ALP DSPP markers showed significantly higher immunoreactivity results confirm nHA/NAR future animal investigations.
Язык: Английский
Процитировано
0Journal of drug targeting, Год журнала: 2025, Номер unknown, С. 1 - 24
Опубликована: Фев. 28, 2025
This study developed an alginate, gelatin, and chondroitin sulfate hydrogel incorporating silicon oxide nanoparticles to assess morphology, cell proliferation, viability. The effectiveness of these hydrogels for cartilage repair was evaluated in vivo using male albino rabbits, divided into three groups: a control group without hydrogels, observer with lacking nanoparticles, treatment nanoparticle-enhanced post-injury repair. At 15, 30, 60 days post-surgery, the rabbits were humanely euthanized, excised tissue samples fixed 10% formalin histopathological analysis, then processed embedded paraffin microscopic evaluation. Statistical analysis performed SPSS software ANOVA Tukey's post hoc test. Results indicated that supported viability encouraged differentiation chondrocyte-like phenotypes. Scanning Electron Microscopy (SEM) confirmed hydrogels' porosity showed significant differences survival rates compared group, underscoring potential engineering regenerative strategies.
Язык: Английский
Процитировано
0Ophthalmology, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
To compare the early exposure and surgical revision rates between a new synthetic tissue substitute (CorNeat EverPatch) with that of human donor cornea following placement onto scleral surface during ophthalmic surgery, to study biomaterial properties patch material. Retrospective comparison study; analyses explanted All consecutive patients who underwent surgery implantation CorNeat EverPatch at Wilmer Eye Institute (occurring from February August 2024) group irradiated cornea, matched 1:2 receiving for age, type glaucoma, surgeon. review clinical electronic medical records Institute. Materials characterization including morphology, roughness, wettability, thermal stability, elemental analysis, physical properties. Early (within 5 months surgery) or surgery. Thirty undergoing in 2024 received primary tube shunt (N=27), (N=2), covering exposed suture used fixation an intraocular lens (N=1). During postoperative period, rate was 48.3% 27.9%. In case-matched control (N=58), graft 1.7% (p<0.0001) (p<0.0001). devices were comprised randomly aligned fibrous mesh average fiber diameter 1.36 ± 0.78 μm, roughness 1.3 0.1 pore size 3.7 0.4 μm2, percent porosity 37 3%. Explanted demonstrated varying degrees integration significantly increased wettability changes stability composition. The conjunctival complications leading higher than cases involving corneal grafts.
Язык: Английский
Процитировано
0International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2520 - 2520
Опубликована: Март 11, 2025
Melatonin, a natural hormone with antioxidant, anti-inflammatory, and regenerative properties, has gained increasing attention in tissue engineering for its ability to enhance the therapeutic potential of biopolymeric scaffolds. These scaffolds, designed mimic extracellular matrix, provide structural support bioactive environment regeneration. By integrating melatonin, researchers aim create multifunctional scaffolds that promote cell proliferation, modulate inflammatory responses, improve wound healing outcomes. Challenges utilizing melatonin include maintaining stability under light, heat, oxygen exposure, optimizing release profile sustained effects. Innovative fabrication methods, such as electrospinning, 3D printing, lyophilization, have enabled precise control over scaffold architecture delivery. techniques ensure enhanced interactions target tissues tailored regeneration processes. Combining growth factors, cytokines, antimicrobial agents offers applications, from chronic management bone nerve Continued research this field promises transformative solutions medicine, expanding clinical applicability melatonin-enriched This review highlights current progress, challenges, opportunities associated harnessing melatonin’s within frameworks.
Язык: Английский
Процитировано
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