Endoplasmic Reticulum Stress and Its Impact on Adipogenesis: Molecular Mechanisms Implicated

Nutrients, Год журнала: 2023, Номер 15(24), С. 5082 - 5082

Опубликована: Дек. 12, 2023

Endoplasmic reticulum (ER) stress plays a pivotal role in adipogenesis, which encompasses the differentiation of adipocytes and lipid accumulation. Sustained ER has potential to disrupt signaling unfolded protein response (UPR), thereby influencing adipogenesis. This comprehensive review illuminates molecular mechanisms that underpin interplay between We delve into dysregulation UPR pathways, namely, IRE1-XBP1, PERK ATF6 relation adipocyte differentiation, metabolism, tissue inflammation. Moreover, we scrutinize how impacts key adipogenic transcription factors such as proliferator-activated receptor γ (PPARγ) CCAAT-enhancer-binding proteins (C/EBPs) along with their interaction other pathways. The cellular ramifications include alterations adipokines, aged adipose also discuss roles chaperones cyclophilin A B play By shedding light on intricate relationship this paves way for devising innovative therapeutic interventions.

Язык: Английский

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Mesenchymal stem cells for cartilage regeneration: Insights into molecular mechanism and therapeutic strategies

Tissue and Cell, Год журнала: 2024, Номер 88, С. 102380 - 102380

Опубликована: Апрель 10, 2024

Язык: Английский

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EGCG Prevents the Onset of an Inflammatory and Cancer-Associated Adipocyte-like Phenotype in Adipose-Derived Mesenchymal Stem/Stromal Cells in Response to the Triple-Negative Breast Cancer Secretome

Nutrients, Год журнала: 2022, Номер 14(5), С. 1099 - 1099

Опубликована: Март 5, 2022

Triple-negative breast cancer (TNBC) cells secretome induces a pro-inflammatory microenvironment within the adipose tissue, which hosts both mature adipocytes and adipose-derived mesenchymal stem/stromal (ADMSC). The subsequent acquisition of cancer-associated adipocyte (CAA)-like phenotype is, however, unknown in ADMSC. While epidemiological studies suggest that consuming polyphenol-rich diet reduces incidence some obesity-related cancers, chemopreventive impact green tea-derived epigallocatechin-3-gallate (EGCG) against cues trigger CAA remain undocumented Human ADMSC were exposed to human TNBC-derived MDA-MB-231 conditioned media (TNBC secretome) supplemented or not with EGCG. Differential gene expression was assessed through RNA-Seq analysis confirmed by RT-qPCR. Protein levels activation status signal transduction pathways mediators determined Western blotting. chemotaxis real-time cell migration assay. TNBC induced cytokines CCL2, CCL5, IL-1β, IL-6, immunomodulators COX2, HIF-1α, VEGFα, PD-L1. epithelial-to-mesenchymal biomarker Snail found control phenotype. EGCG inhibited induction genes Smad2 NF-κB. chemotactic response also an inflammatory CAA-like can be triggered secretome, while still efficiently prevented diet-derived polyphenols.

Язык: Английский

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Factors affecting osteogenesis and chondrogenic differentiation of mesenchymal stem cells in osteoarthritis

World Journal of Stem Cells, Год журнала: 2023, Номер 15(6), С. 548 - 560

Опубликована: Июнь 26, 2023

Osteoarthritis (OA) is a common degenerative joint disease that often involves progressive cartilage degeneration and bone destruction of subchondral bone. At present, clinical treatment mainly for pain relief, there are no effective methods to delay the progression disease. When this progresses advanced stage, only option most patients total knee replacement surgery, which causes great anxiety. As type stem cell, mesenchymal cells (MSCs) have multidirectional differentiation potential. The osteogenic chondrogenic MSCs can play vital roles in OA, as they relieve improve function. direction accurately controlled by variety signaling pathways, so many factors affect acting on these pathways. applied OA treatment, microenvironment joints, injected drugs, scaffold materials, source other exert specific impacts MSCs. This review aims summarize mechanisms influence MSC produce better curative effects when clinically future.

Язык: Английский

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Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients

Biomedicines, Год журнала: 2023, Номер 11(3), С. 738 - 738

Опубликована: Март 1, 2023

This study aims to identify the role of subjective factors (age, sex, and comorbidities) procedure-specific (aspiration volume) in influencing yield progenitor cells bone marrow aspiration concentrate (BMAC) harvested from iliac crest. A retrospective analysis was conducted on 58 patients (male:female = 31:27; mean age: 52.56 ± 18.14 years) who underwent BMAC therapy between January 2020 June 2021. The analyzed include individual such as age, comorbid conditions, procedural aspirate volume. mononuclear cell (MNC) count colony-forming unit (CFU) assay were used assess progenitors aspirate. Pearson's correlation test performed for volume, outcome parameters, MNC CFU. We chi-square analyze sex comorbidities cellular yield. volume 66.65 (±17.82) mL. 19.94 (±16.34) × 106 cells, which formed 11 (±12) CFUs. Evidence statistically significant positive associations noted CFUs developed within them (r 0.95, p < 0.001). did not play any (p 0.092) or 0.448). age showed evidence a negative association with -0.681, 0.001) -0.693, 0.001), -0.740, -0.629, also reduction 0.002) 0.004) when presented comorbidities. Individual procedure significantly affected BMAC. influence

Язык: Английский

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3D Bioprinting of Biomimetic Alginate/Gelatin/Chondroitin Sulfate Hydrogel Nanocomposites for Intrinsically Chondrogenic Differentiation of Human Mesenchymal Stem Cells

Biomacromolecules, Год журнала: 2024, Номер 25(6), С. 3312 - 3324

Опубликована: Май 10, 2024

3D-printed hydrogel scaffolds biomimicking the extracellular matrix (ECM) are key in cartilage tissue engineering as they can enhance chondrogenic differentiation of mesenchymal stem cells (MSCs) through presence active nanoparticles such graphene oxide (GO). Here, biomimetic hydrogels were developed by cross-linking alginate, gelatin, and chondroitin sulfate biopolymers GO a bioactive filler, with excellent processability for developing 3D printed bioprinting process. A novel bioink based on our embedded human MSCs presented cell survival rate near 100% after The effects processing filler concentration further quantitatively evaluated. nanocomposited render high MSC proliferation viability, exhibiting intrinsic chondroinductive capacity without any exogenous factor when used to print or bioprint constructs. bioactivity depended concentration, best performance at 0.1 mg mL–1. These results explained rational combination three biopolymers, having carboxylate groups their structures, therefore, highly negatively charged ECM cartilage. this biomaterial its good printing techniques open up new approach materials repair.

Язык: Английский

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Stem Cell Therapies in Alzheimer’s Disease: Applications for Disease Modeling

Journal of Pharmacology and Experimental Therapeutics, Год журнала: 2021, Номер 377(2), С. 207 - 217

Опубликована: Фев. 8, 2021

Alzheimer's disease (AD) is a neurodegenerative with complex pathologic and biologic characteristics. Extracellular β-amyloid deposits, such as senile plaques, intracellular aggregation of hyperphosphorylated tau, neurofibrillary tangles, remain the main neuropathological criteria for diagnosis AD. There currently no effective treatment disease, many clinical trials have failed to prove any benefits new therapeutics. More recently, there has been increasing interest in harnessing potential stem cell technologies drug discovery, modeling, therapies, which used study an array human conditions, including The recently developed optimized induced pluripotent (iPSC) technology critical platform screening anti-AD drugs understanding mutations that modify Neural (NSC) transplantation investigated therapeutic approach treat diseases. Mesenchymal cells (MSCs) also exhibit considerable diseases by secreting growth factors exosomes, attenuating neuroinflammation. This review highlights recent progress research translational applications challenges iPSCs, NSCs, MSCs strategies Even though these treatments are still relative infancy, developing hold promise combat AD other disorders.

SIGNIFICANCE STATEMENT

results learning memory defects. Although some approved treatment, fewer than 20% patients benefit from drugs. Therapies based on cells, neural mesenchymal provide promising

Язык: Английский

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A bi-layered membrane with micro-nano bioactive glass for guided bone regeneration

Colloids and Surfaces B Biointerfaces, Год журнала: 2021, Номер 205, С. 111886 - 111886

Опубликована: Май 30, 2021

Язык: Английский

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Autophagy, Mesenchymal Stem Cell Differentiation, and Secretion

Biomedicines, Год журнала: 2021, Номер 9(9), С. 1178 - 1178

Опубликована: Сен. 7, 2021

Mesenchymal stem cells (MSC) are multipotent capable to differentiate into adipogenic, osteogenic, and chondrogenic directions, possessing immunomodulatory activity a capability stimulate angiogenesis. A scope of these features capabilities makes MSC significant factor tissue homeostasis repair. Among factors determining the fate MSC, prominent place belongs autophagy, which is activated under different conditions including cell starvation, inflammation, oxidative stress, some others. In addition supporting by elimination protein aggregates, non-functional damaged proteins, autophagy necessary change in phenotype on process differentiation. present review, mechanisms providing participation differentiation discussed

Язык: Английский

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Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame

Cellular and Molecular Life Sciences, Год журнала: 2022, Номер 79(11)

Опубликована: Окт. 6, 2022

Stem cells undergo cytokine-driven differentiation, but this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing 'model cells' (apoptotic differentiated cells) was found require only a short time frame. Pluripotent-like Muse cells, multipotent mesenchymal (MSCs), and neural (NSCs) phagocytosed apoptotic different phagocytic receptor subsets macrophages. The phagocytosed-differentiated cell-derived contents (e.g., transcription factors) were quickly released into the cytoplasm, translocated nucleus, bound promoter regions of genomes. Within 24 ~ 36 h, expressed lineage-specific markers corresponding both in vitro vivo. At 1 week, gene expression profiles similar those authentic functional markers. Differentiation limited inherent potential each line: triploblastic-, adipogenic-/chondrogenic-, neural-lineages MSCs, NSCs, respectively. Disruption phagocytosis, either by inhibition small interfering RNA or annexin V treatment, impeded Together, our findings uncovered simple which differentiation-directing factors are directly transferred trigger their rapid target lineage.

Язык: Английский

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