Intestinal epithelial barrier integrity investigated by label-free techniques in ulcerative colitis patients DOI Creative Commons

Elsie Quansah,

Elena Gardey,

Anuradha Ramoji

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Авг. 23, 2022

Abstract The intestinal epithelial barrier, among other compartments such as the mucosal immune system, contributes to maintenance of homeostasis. Therefore, any disturbance within layer could lead permeability and promote inflammation. Considering that disintegration barrier is a key element in etiology ulcerative colitis, further assessment integrity contribute better understanding role defects colitis (UC), one major form chronic inflammatory bowel disease. Herein, we employ fast, non-destructive, label-free non-linear methods, namely coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), two-photon excited fluorescence (TPEF), lifetime imaging (2P-FLIM) assess morpho-chemical contributions leading dysfunction barrier. For first time, formation gaps was directly visualized, without sophisticated data analysis procedures, by 3D colonic mucosa from severely inflamed UC patients. results were compared with histopathological immunofluorescence images validated using transmission electron microscopy (TEM) indicate structural alterations apical junction complex underlying cause for gaps. Our findings suggest potential multimodal give precise, detailed, direct visualization gastrointestinal tract, which can be combined fiber probe future endomicroscopy measurements during real-time in-vivo imaging.

Язык: Английский

Cancer Metastasis‐on‐a‐Chip for Modeling Metastatic Cascade and Drug Screening DOI Creative Commons
Anastasia Brooks, Yali Zhang, Jiezhong Chen

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(21)

Опубликована: Янв. 15, 2024

Microfluidic chips are valuable tools for studying intricate cellular and cell-microenvironment interactions. Traditional in vitro cancer models lack accuracy mimicking the complexities of vivo tumor microenvironment. However, cancer-metastasis-on-a-chip (CMoC) combine advantages 3D cultures microfluidic technology, serving as powerful platforms exploring mechanisms facilitating drug screening. These able to compartmentalize metastatic cascade, deepening understanding its underlying mechanisms. This article provides an overview current CMoC models, focusing on distinctive that simulate invasion, intravasation, circulation, extravasation, colonization, their applications Furthermore, challenges faced by technologies discussed, while promising future directions research. The ongoing development integration these into studies expected drive transformative advancements field.

Язык: Английский

Процитировано

11
Fecal and Circulating Biomarkers for the Non-Invasive Assessment of Intestinal Permeability DOI Creative Commons
Nuria Pérez-Díaz-del-Campo, Gabriele Castelnuovo, Davide Giuseppe Ribaldone

и другие.

Diagnostics, Год журнала: 2023, Номер 13(11), С. 1976 - 1976

Опубликована: Июнь 5, 2023

The study of intestinal permeability is gaining growing interest due to its relevance in the onset and progression several gastrointestinal non-gastrointestinal diseases. Though involvement impaired pathophysiology such diseases recognized, there currently a need identify non-invasive biomarkers or tools that are able accurately detect alterations barrier integrity. On one hand, promising results have been reported for novel vivo methods based on paracellular probes, i.e., can directly assess and, other fecal circulating indirectly epithelial integrity functionality. In this review, we aimed summarize current knowledge transport pathways provide an overview already available under investigation measurement permeability.

Язык: Английский

Процитировано

20
SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation DOI Creative Commons
Kevin Schmidt, Arne Schmidt,

Sonja Groß

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 16, 2024

Beneficial effects of sodium glucose co-transporter 2 inhibitors (SGLT2is) in cardiovascular diseases have been extensively reported leading to the inclusion these drugs treatment guidelines for heart failure. However, molecular actions especially on non-myocyte cells remain uncertain. We observed dose-dependent inhibitory two SGLT2is, dapagliflozin (DAPA) and empagliflozin (EMPA), inflammatory signaling human umbilical vein endothelial cells. Proteomic analyses subsequent enrichment discovered profound SGLT2is proteins involved mitochondrial respiration actin cytoskeleton. Validation functional oxygen consumption measurements as well tube formation migration assays revealed strong impacts DAPA. Considering that most influenced parameters played central roles mesenchymal transition (EndMT), we performed vitro EndMT identified substantial reduction fibrosis marker expression changes cellular morphology upon with SGLT2is. In line, cardiac fibroblasts exposed DAPA showed less proliferation, reduced ATP production, decelerated capacity while extensive were EMPA. Mechanistically, proton exchanger 1 (NHE1) sodium-myoinositol cotransporter (SMIT) sodium-multivitamin (SMVT) could be relevant targets validated by individual siRNA-knockdown experiments. summary, found comprehensive beneficial fibroblasts. The results this study therefore support a distinct effect selected SGLT2i grant further insights into potential mode action drugs.

Язык: Английский

Процитировано

7
Intestinal epithelial barrier integrity investigated by label-free techniques in ulcerative colitis patients DOI Creative Commons

Elsie Quansah,

Elena Gardey,

Anuradha Ramoji

и другие.

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Фев. 15, 2023

Abstract The intestinal epithelial barrier, among other compartments such as the mucosal immune system, contributes to maintenance of homeostasis. Therefore, any disturbance within layer could lead permeability and promote inflammation. Considering that disintegration barrier is a key element in etiology ulcerative colitis, further assessment integrity contribute better understanding role defects colitis (UC), one major form chronic inflammatory bowel disease. Herein, we employ fast, non-destructive, label-free non-linear methods, namely coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), two-photon excited fluorescence (TPEF), lifetime imaging (2P-FLIM), assess morpho-chemical contributions leading dysfunction barrier. For first time, formation gaps was directly visualized, without sophisticated data analysis procedures, by 3D colonic mucosa from severely inflamed UC patients. results were compared with histopathological immunofluorescence images validated using transmission electron microscopy (TEM) indicate structural alterations apical junction complex underlying cause for gaps. Our findings suggest potential advantage multimodal give precise, detailed, direct visualization gastrointestinal tract, which can be combined fiber probe future endomicroscopy measurements during real-time vivo imaging.

Язык: Английский

Процитировано

14
The role of cell–cell and cell–matrix junctional complexes in sebaceous gland homeostasis and differentiation DOI Creative Commons
Aylin Yaba, Torsten Thalheim, Marlon R. Schneider

и другие.

Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)

Опубликована: Сен. 23, 2024

Язык: Английский

Процитировано

3
Poly(vinyl alcohol) reduced and capped gold nanoparticles as contrast enhancers to target and improve detection of medial calcification DOI Creative Commons
Tao Song, Kyoungmi Bak,

Dahyun Kyung

и другие.

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Medial calcification is the pathological deposition of calcium phosphate (CaP) minerals in elastin-rich medial layers arteries, leading to vessel stiffening and increased risk heart failure. There are no drugs treat calcification, thus it would be important detect disease as early possible enable adequate prevention. In clinic, X-ray based computed tomography (CT) used diagnose but few small CaP present stages do not provide enough contrast detectable by CT. Herein, we propose poly(vinyl alcohol) (PVA) reduced capped gold nanoparticles (PVA@AuNPs) target improve its detection stages. AuNPs can greatly absorb X-rays work enhancers for Results show that PVA@AuNPs bind containing hydroxyl ions on their surface, most likely via hydrogen-bond interactions with PVA capping polymers; indeed, mineral binding efficiency depends hydrolysis degree PVA. prepared from 99%+ hydrolyzed (PVA99@AuNPs) selectively calcified vs. non-calcified elastin vitro, vivo they 4-week-old matrix Gla-protein deficient mice imaged through micro-CT. STATEMENT OF SIGNIFICANCE: The clinical (CT). improving (micro)CT detection. Unlike previously proposed targeting agents, PVA99@AuNPs phases early-stage extracellular onto which deposited, enabling accurate specific targeting. Their straightforward synthesis biocompatibility significantly enhance potential translation. Earlier management, particularly since treatments available disease.

Язык: Английский

Процитировано

0
Extending Imaging Volume in Soft X‐Ray Tomography DOI Creative Commons
Axel Ekman, Jian-Hua Chen, Bieke Vanslembrouck

и другие.

Advanced Photonics Research, Год журнала: 2023, Номер 4(4)

Опубликована: Март 2, 2023

Soft X‐ray tomography offers rapid imaging of whole, single cells with a few tens nanometers spatial resolution without fixation or labeling. Herein, this technique is limited to specimens about 10 μm thick, such that applications soft large human multicellular are not possible. A theoretical and experimental framework for enables extension volumes 18 μm‐thick developed. This approach, based on long depth field half‐acquisition tomography, easily applicable microscopes equipped full‐rotation specimen stage. opens opportunities cells, as those commonly seen in cancer research, well cell‐to‐cell interactions, where two more occupy the same volume.

Язык: Английский

Процитировано

7
Regulation of Epithelial and Endothelial Barriers by Molecular Chaperones DOI Creative Commons
Susana Lechuga,

Armando Marino‐Melendez,

Nayden G. Naydenov

и другие.

Cells, Год журнала: 2024, Номер 13(5), С. 370 - 370

Опубликована: Фев. 21, 2024

The integrity and permeability of epithelial endothelial barriers depend on the formation tight junctions, adherens a junction-associated cytoskeleton. establishment this junction–cytoskeletal module relies correct folding oligomerization its protein components. Molecular chaperones are known regulators complex in different cellular compartments. Mammalian cells possess an elaborate chaperone network consisting several hundred co-chaperones. Only small part has been linked, however, to regulation intercellular adhesions, systematic analysis functions at is lacking. This review describes mechanisms chaperone-assisted junctions. major focus heat shock chaperones, their co-chaperones, chaperonins since these molecules majority articles published chaperone-mediated control tissue barriers. discusses roles steady-state vascular as well disruption by pathogenic factors extracellular stressors. Since cytoskeletal coupling essential for junctional remodeling, assembly actomyosin cytoskeleton also discussed.

Язык: Английский

Процитировано

2
Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions DOI Creative Commons

Caroline S. Cencer,

Jennifer B. Silverman, Leslie M. Meenderink

и другие.

Developmental Cell, Год журнала: 2023, Номер 58(20), С. 2048 - 2062.e7

Опубликована: Окт. 1, 2023

Язык: Английский

Процитировано

5
The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices DOI Creative Commons
Martina Nalezinková, Jan Loskot, Alena Fučíková

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Фев. 26, 2024

Testing the hemocompatibility of medical devices after their interaction with blood entails need to evaluate activation elements and degree coagulation adhesion device surface. One possible way achieve this is use scanning electron microscopy (SEM). The aim was develop a novel SEM-based method assess thrombogenic potential adhesiveness cells. As part task, also find convenient procedure efficient non-destructive sample fixation for SEM while reducing highly toxic substances shortening time. A polymeric surgical mesh exposed so that adhered its Such prepared samples were then chemically fixed subsequent measurement; number procedures tested optimal one. results evaluated from images, elements' determined images using ImageJ software. best achieved May-Grünwald solution, which less than chemicals traditionally used. Moreover, manipulation osmium tetroxide can be avoided in proposed procedure. methodology image analysis has been developed too, enabling quantitatively surfaces various devices. Our replaces subjective assessment surface coverage better-defined procedure, thus offering more precise reliable results.

Язык: Английский

Процитировано

1