Effect of Octopamine on the Frequency Tuning of the Auditory System in Culex Pipiens Pipiens Mosquito (Diptera, Culicidae)

Neuroscience and Behavioral Physiology, Год журнала: 2024, Номер 54(2), С. 319 - 328

Опубликована: Фев. 1, 2024

Язык: Английский

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Flupyradifurone, imidacloprid and clothianidin disrupt the auditory processing in the locust CNS

Journal of Comparative Physiology A, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

Abstract Since the EU banned classic neonicotinoids like imidacloprid and clothianidin, they may be replaced by more recently marketed insecticides such as flupyradifurone. However, all operate on same neuropharmacological principle selective agonists at insect’s nicotinic acetylcholine receptors. Here we investigated impact of flupyradifurone, clothianidin neuronal processing in auditory pathway desert locust Schistocerca gregaria . While stepwise increasing insecticide concentration haemolymph, extracellularly recorded spike responses afferents tympanal nerve interneurons neck connectives. All three showed a very similar dose-dependent suppression ascending towards brain, whereas sensory neurons ears appeared unaffected. Furthermore, systematic injection experiments demonstrate that dosages which already supress information transfer are far too low to induce typical poisoning symptoms trembling, spasms, paralysis. We discuss how sublethal intoxication with classical or functionally related flupyradifurone disrupt postsynaptic balance between excitation inhibition locusts other orthopteran insects.

Язык: Английский

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The octopamine receptor OAα1 influences oogenesis and reproductive performance in Rhodnius prolixus

PLoS ONE, Год журнала: 2023, Номер 18(12), С. e0296463 - e0296463

Опубликована: Дек. 29, 2023

The control of reproductive processes in Rhodnius prolixus involves a variety neuroactive chemicals. Among these, several studies have suggested that the biogenic amine octopamine (OA), might play an active role these processes. Here, we investigate molecular profile R . α adrenergic-like OA receptor 1 (RpOAα1-R) and its egg production. Comparative analyses confirm RpOAα1-R gene codes for true OAα1 receptor. transcript is highly expressed tissues associated with production, after blood meal, which stimulus full production , upregulated ovaries spermatheca. After RNAi-mediated knockdown, ovarian phenotype characterized by slow development observed. Furthermore, altered has been eggs are deformed. Interestingly, there no evidence disruption vitellogenin (Vg) synthesis fat body or uptake oocytes. On other hand, downregulation correlated defective choriogenesis eggs. These results provide critical information concerning OAα1-R oogenesis

Язык: Английский

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Transgenic line for characterizing GABA-receptor expression to study the neural basis of olfaction in the yellow-fever mosquito

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Март 28, 2024

The mosquito Aedes aegypti is an important vector of diseases including dengue, Zika, chikungunya, and yellow fever. Olfaction a critical modality for mosquitoes enabling them to locate hosts, sources nectar, sites oviposition. GABA essential neurotransmitter in olfactory processing the insect brain, primary center, antennal lobe. Previous work with Ae. has suggested that lobe inhibition via may be involved odors. However, little known about receptor expression or how they odor attraction. In this context, generating mutants target mosquito’s responses, particularly GABAergic system, achieve better understanding these diverse processes coding disease vectors. Here we demonstrate potential transgenic line using QF2 transcription factor, GABA-B1 QF2−ECFP , as new neurogenetic tool investigate neural basis olfaction aegypti. Our results show gene insertion moderate impact on fitness. Moreover, presented here was crossed QUAS reporter expressing green fluorescent protein used determine location metabotropic expression. We find high lobes, especially cell bodies surrounding lobes. mushroom bodies, Kenyon cells, but had low body Behavioral experiments testing fruit attractants showed lost their behavioral Together, provides characterize systems nervous system.

Язык: Английский

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Differences in male Aedes aegypti and Aedes albopictus hearing systems facilitate recognition of conspecific female flight tones

iScience, Год журнала: 2024, Номер 27(7), С. 110264 - 110264

Опубликована: Июнь 13, 2024

When

Язык: Английский

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The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

Malaria mosquitoes mate in crowded, and noisy, swarms. A vital stage of their precopulatory behaviour involves detecting the faint flight tone a mating partner amidst noise from hundreds other mosquitoes. This exquisite sensory performance is enabled by complex auditory system with remarkable features. One such feature vast efferent control system, which provides mosquito ear required plasticity to adapt various external internal changes. In this paper, we study role GABA, one main signalling molecules ear, its interactions octopamine, another neurotransmitter network. We show that GABA released malaria nerve receptors are expressed including GABAA receptor Resistance Dieldrin (RDL), target for evolution insecticide resistance. Using picrotoxin antagonize RDL receptors, discovered multiple effects at mechanical electrical level. At level, blocking promotes erection antennal fibrillae cessation flagellar self-sustained oscillations. These not observed knockouts octopamine AgOctbeta2, suggesting mediated through release ear. Electrically, injection increases spontaneous firing neurons direct current (DC) responses stimulation both wildtype AgOctbeta2 mutants, indicating may also modulate sensitivity via octopamine-independent pathways. summary, our experiments uncover distinct roles as well synergistic octopamine. The data fundamental controlling function implicate natural populations.

Язык: Английский

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Perspectives on the manipulation of mosquito hearing

Current Opinion in Insect Science, Год журнала: 2024, Номер unknown, С. 101271 - 101271

Опубликована: Сен. 1, 2024

Язык: Английский

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Effect of octopamine on the frequency tuning of the auditory system in <i>Culex pipiens pipiens</i> mosquito (Diptera, Culicidae)

Сенсорные системы, Год журнала: 2023, Номер 37(3), С. 244 - 257

Опубликована: Июль 1, 2023

Язык: Английский

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Acoustic Communication in Fruit Flies and Mosquitoes

Опубликована: Янв. 1, 2023

Acoustic communication between conspecific males and females, mediated by wing movements, forms a key part of premating behavior in both fruit flies mosquitoes. However, there are substantial differences how, where, when this is conducted, resulting distinct ear anatomy function. Here, we compare acoustic behaviors the two groups. Given relative lack genetic tools available until recently mosquitoes, much remains unclear regarding fundamental underpinnings hearing. We describe neural circuitry underlying hearing flies, how equivalent mosquito can be investigated future.

Язык: Английский

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Monoaminergic Systems in Flight-Induced Potentiation of Phonotactic Behavior in Female Crickets Gryllus bimaculatus

Insects, Год журнала: 2024, Номер 15(3), С. 183 - 183

Опубликована: Март 9, 2024

We have recently shown that experience of flight remarkably enhanced subsequent terrestrial phonotaxis in females response to the male calling song. Here, we elucidated possible roles octopamine and serotonin enhancing effect flying on phonotactic behavior. Octopamine is known be released into hemolymph during insects; however, receptor antagonist epinastine did not abolish effects our study. On contrary, drug significantly potentiated influence The agonist chlordimeform, at a concentration 2 mM, which was previously found activate aggression crickets, dramatically reduced response. However, 10-times-lower dose, chlordimeform produced light but significant decrease time took reach source A similar by itself, hardly passes blood–brain barrier insects. completely abolished female crickets treated with alpha-methyl tryptophan (AMTP). AMPT suppresses synthesis serotonin, decreasing its content nervous systems insects, including crickets. An activation 5-hydroxytryptophan mimicked increasing number visits spent zone near 5-HT third thoracic ganglion higher flyers compared control group. In contrast, no changes level were observed ganglion, play crucial role decision-making involved intraspecific interactions. Therefore, results suggest although flight, it likely inhibit rather than central mechanisms related phonotaxis. weak facilitating low dose can attributed peripheral octopaminergic receptors. Our serotoninergic system may contribute facilitation behavior flying. both enhance sexual motivation and, these means, impact their behavioral

Язык: Английский

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