Journal of Investigative Dermatology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Communications Biology, Год журнала: 2024, Номер 7(1)
Опубликована: Ноя. 19, 2024
Abstract Wound healing is a highly coordinated spatiotemporal sequence of events involving several cell types and tissues. The process wound requires strict regulation, its disruption can lead to the formation chronic wounds, which have significant impact on an individual’s health as well worldwide healthcare expenditure. One essential aspect within cellular molecular regulation pathogenesis that reactive oxygen species (ROS) oxidative stress. Wounding significantly elevates levels ROS, array various are involved in modulating process, such through antimicrobial activities signal transduction. However, many pathologies, ROS play antagonistic pleiotropic role healing, be pathogenic factor wounds. Whilst advances targeting stress led development novel pre-clinical therapeutic methods, due complex nature gaps knowledge remain concerning specific functions healing. In this review, we highlight current these functions, discuss potential future direction new studies, how pathways may targeted studies.
Язык: Английский
Процитировано
11
Biomimetics, Год журнала: 2024, Номер 9(5), С. 278 - 278
Опубликована: Май 7, 2024
Biomimetic materials have become a promising alternative in the field of tissue engineering and regenerative medicine to address critical challenges wound healing skin regeneration. Skin-mimetic enormous potential improve outcomes enable innovative diagnostic sensor applications. Human skin, with its complex structure diverse functions, serves as an excellent model for designing biomaterials. Creating effective coverings requires mimicking unique extracellular matrix composition, mechanical properties, biochemical cues. Additionally, integrating electronic functionality into these presents exciting possibilities real-time monitoring, diagnostics, personalized healthcare. This review examines biomimetic their role healing, well integration technologies. It discusses recent advances, challenges, future directions this rapidly evolving field.
Язык: Английский
Процитировано
10
Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)
Опубликована: Май 27, 2024
Abstract Chronic wounds, resulting from persistent inflammation, can trigger a cascade of detrimental effects including exacerbating inflammatory cytokines, compromised blood circulation at the wound site, elevation white cell count, increased reactive oxygen species, and potential risk bacterial infection. The interleukin-17 (IL-17) signaling pathway, which plays crucial role in regulating immune responses, has been identified as promising target for treating skin diseases. This review aims to delve deeper into pathological molecular mechanisms IL-17 family its pathways repair. intricate interactions between other cytokines will be discussed detail, along with activation various pathways, provide comprehensive understanding IL-17’s involvement chronic inflammation
Язык: Английский
Процитировано
8
Cytotherapy, Год журнала: 2024, Номер 26(6), С. 579 - 585
Опубликована: Март 4, 2024
Язык: Английский
Процитировано
6
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Окт. 30, 2024
Phototherapy can offer a safe and non-invasive solution against infections, while promoting wound healing. Conventional phototherapeutic devices are bulky limited to hospital use. To overcome these challenges, we developed wearable, flexible red blue LED (r&bLED) patch controlled by mobile-connected system, enabling self-application at home. The exhibits excellent skin compatibility, flexibility, comfort, with high safety under system supervision. Additionally, synthesized sprayable fibrin gel (F-gel) containing light-sensitive thymoquinone light-synergistic NADH. Combined bLED, eradicated microbes biofilms within minutes, regardless of antibiotic resistance. Furthermore, NADH rLED synergistically improved macrophage endothelial cell mitochondrial function, healing, reducing inflammation, enhancing angiogenesis, as validated in infected diabetic wounds mice minipigs. This innovative technology holds great promise for revolutionizing at-home phototherapy chronic wounds.
Язык: Английский
Процитировано
5
Archives of Dermatological Research, Год журнала: 2025, Номер 317(1)
Опубликована: Фев. 8, 2025
Abstract Previous research has identified a variety of factors that contribute to the development and maintenance wounds. Concurrently, electroacupuncture been demonstrated facilitate wound healing. However, effects transcutaneous electrical acustimulation (TEA) on healing, as well its relationship with key such Wnt3a, TGF-β, Akt, c-Myc, VEGF-A, SP1, nitric oxide (NO), mitochondrial function, remain largely unexplored. We hypothesize TEA will activate signaling enhance functions promote repair skin wounds in mice. An vivo experimental study was conducted utilizing mouse models The comprised three groups: treatment group, model control group. Wound areas were measured by calculating product length width each using calipers. Single-cell suspensions prepared excising immediately surrounding tissue. These stained Trypan blue assess cell viability, specific probes measure rate reactive oxygen species (ROS) positivity, reagents quantify NO content. Western blotting (WB) employed evaluate protein levels associated tissue changes, while quantitative polymerase chain reaction (qPCR) used RNA expression levels. Immunofluorescence staining performed visualize content other relevant cellular structures within sections. exhibited anti-inflammatory properties promoted healing blot analysis revealed enhanced proteins SP1 during process. sections indicated upregulated COL1A1, MFN1, GRP75, GRP78, GRP75/ROS, GRP78/ROS, ISCU, UCP1 downregulating FIS1. Additionally, qPCR results IL-10 miRNA205-5p inhibiting MMP9 modulates various molecules, influences chaperone related stress recovery responses, along dynamics metabolism. Graphical abstract
Язык: Английский
Процитировано
0
Pharmaceutics, Год журнала: 2025, Номер 17(4), С. 453 - 453
Опубликована: Апрель 1, 2025
Background/Objectives: This study aimed to explore the therapeutic potential of umbilical mesenchymal stem cell-derived apoptotic vesicles (UMSC-apoVs) in a 5-Fluorouracil (5-FU)-induced impairment skin wound healing. Methods: UMSC-apoVs were isolated from UMSCs using differential centrifugation after induction apoptosis. A murine model was established by administering 5-FU via intravenous tail injection, followed full-thickness creation. Mice received local injections at lesion site. Wound healing evaluated based on closure rates, histological analysis, and vivo/in vitro functional assays. Rotenone (Rot)-pretreated used role mitochondrial transfer between cells (SMSCs) Results: significantly accelerated 5-FU-treated mice, as demonstrated enhanced rates findings reduced inflammatory infiltration increased collagen deposition. transferred mitochondria SMSCs, enhancing viability, proliferation, migration while reducing reactive oxygen species (ROS) production SMSCs. Rot pretreatment inhibited effects inducing dysfunction UMSC-apoVs. Conclusions: Our indicate that improve 5-FU-induced impaired facilitating transfer, suggesting novel strategy for alleviating chemotherapy-induced
Язык: Английский
Процитировано
0
JCI Insight, Год журнала: 2024, Номер 9(9)
Опубликована: Апрель 2, 2024
Central for wound healing is the formation of granulation tissue, which largely consists collagen and whose importance stretches past healing, including being implicated in both fibrosis skin aging. Cyclophilin D (CyD) a mitochondrial protein that regulates permeability transition pore, known its role apoptosis ischemia-reperfusion. To date, CyD human generation has been unexplored. Here, we show was upregulated normal wounds venous ulcers, likely adaptive as inhibition impaired reepithelialization, tissue formation, closure pig models. Overexpression increased keratinocyte migration fibroblast proliferation, while reduced migration. Independent fibroblasts secretion caused endoplasmic reticulum accumulation, overexpression secretion. This confirmed Ppif-KO mouse model, showed reduction collagen. Overall, this study revealed previously unreported roles skin, with implications beyond.
Язык: Английский
Процитировано
4
Next research., Год журнала: 2025, Номер unknown, С. 100160 - 100160
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Archives of Biotechnology and Biomedicine, Год журнала: 2025, Номер 9(1), С. 001 - 009
Опубликована: Янв. 31, 2025
Mitochondria are essential intracellular organelles that significantly influence various cellular processes, including metabolism, stress response, and cell fate. Their precise regulation is crucial for maintaining both organelle homeostasis. Wound healing a complex, multifactorial process relies on the coordinated actions of multiple types numerous mechanisms. Dysregulation in this can lead to chronic wounds, which pose substantial challenges healthcare systems present limited treatment options due their intricate pathogenesis. Recent research has increasingly focused role mitochondria wound healing, revealing involvement critical processes such as apoptosis, redox signaling. Mitochondrial dynamics play vital by adapting demands environmental cues. Moreover, mitophagy, selective degradation damaged mitochondria, mitochondrial integrity function during process. not only pivotal energy production but also calcium homeostasis generation reactive oxygen species, signaling repair. As progresses through distinct yet overlapping stages facilitate repair contribute cytoskeletal remodeling necessary closure. Understanding multifaceted roles could novel therapeutic approaches wounds. Future should prioritize investigating functions human tissues develop targeted strategies enhancing outcomes.
Язык: Английский
Процитировано
0