Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Сен. 13, 2024
Previous research has demonstrated an association between gut microbiota and immune status with the development of several diseases. However, whether these factors contribute to polyps remains unclear. This study aims use Mendelian randomization (MR) investigate causal relationship 4 types (nasal, gallbladder, colon, gastric polyps), as well analyze mediating role traits.
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7937 - 7937
Опубликована: Июль 20, 2024
The gut microbiota has acquired significant attention in recent years for its potential as a diagnostic biomarker colorectal cancer (CRC). In this literature review, we looked at the studies exploring alterations composition associated with CRC, mechanisms linking dysbiosis to CRC development, and approaches utilizing analysis. Our research led conclusion that individuals often display their compared healthy individuals. These can include changes diversity, abundance, type of bacteria present gut. While use holds promise, further is needed validate effectiveness standardize testing protocols. Additionally, considerations such variability among factors must be addressed before microbiota-based tests widely implemented clinical practice.
Язык: Английский
Процитировано
12
Gut Pathogens, Год журнала: 2025, Номер 17(1)
Опубликована: Фев. 2, 2025
Язык: Английский
Процитировано
2
BMC Microbiology, Год журнала: 2024, Номер 24(1)
Опубликована: Июль 18, 2024
Abstract Background More than 90% of colorectal cancer (CRC) arises from advanced adenomas (AA) and gut microbes are closely associated with the initiation progression both AA CRC. Objective To analyze characteristic in AA. Methods Fecal samples were collected 92 184 negative control (NC). Illumina HiSeq X sequencing platform was used for high-throughput microbial populations. The results annotated compared NCBI RefSeq database to find characteristics R-vegan package α diversity β diversity. included box diagram, Principal Component Analysis (PCA), principal co-ordinates analysis (PCoA), non-metric multidimensional scaling (NMDS). risk prediction models constructed based on six kinds machine learning algorithms. In addition, unsupervised clustering methods classify bacteria viruses. Finally, viruses different subtypes analyzed. Results abundance Prevotella sp900557255 , Alistipes putredinis Megamonas funiformis higher AA, while Lilyvirus Felixounavirus Drulisvirus also Catboost model predicting has highest accuracy (bacteria test set: 87.27%; virus 83.33%). 4 (B1V1, B1V2, B2V1, B2V2) distinguished enteroviruses (EVs). Escherichia coli D CAG-180 sp000432435 Phocaeicola plebeiuA Teseptimavirus Svunavirus Jiaodavirus subtypes. indicated that improved after incorporating discovery sets 100%, 96.34%, 98.46% subtypes, respectively. Conclusion have high value early warning As promising non-invasive biomarkers, can become potential diagnostic targets be by typing.
Язык: Английский
Процитировано
9
Frontiers in Oncology, Год журнала: 2025, Номер 14
Опубликована: Янв. 6, 2025
The current understanding of colorectal carcinogenesis is based on the adenoma-carcinoma sequence, where genetics, intestinal microbiota changes and local immunity shifts seem to play key roles. Despite emerging evidence dysbiotic state immune-cell infiltration in patients with adenocarcinoma, early advanced adenoma as precursors cancer, carcinoma situ following progression, are rather less studied. newly colon-site adapted AI-based analysis immune infiltrates able predict long-term outcomes colon carcinoma. Though it could also facilitate pathologic evaluation precancerous lesion's potential progress. Therefore, purpose this prospective cohort study (MIMICA-1) is, firstly, identify patterns around normal bowel tissue, adenoma, situ, secondly, analyze - microbiome interplay along steps conventional tumorigenesis. This aims prospectively recruit 40 (10 per group) confirmed dysplasia undergoing endoscopic polypectomy, mucosal resection for small (≤1cm), large (>1cm) or biopsy subsequent invasive 10 healthy screening colonoscopy. Stool samples will be collected prior preparation fecal (luminal) composition. Biopsy specimens taken from terminal ileum, right colon, left a pathological lesion (if present) assess mucosa-associated composition response. DNA extracted all sequenced using Illumina MiSeq platform. Unifrac Bray-Curtis methods used microbial diversity. system response examined digital image primarily immunohistochemistry procedures CD3, CD8, CD20 CD68 cell markers performed. Thereafter, count, density distribution immunocompetent cells epithelial stromal tissue compartments evaluated interaction between sequence examined. In addition, explored gut microbiota's composition, comparing fecal- tissue-derived bacterial sequence. We hypothesize that within human led detectable alterations correlate progression mucosa It expectable find more severe at site, though analyzing cancer we expect detect broader luminal near-lesion site possibly throughout entire colon. believe specific compositional differences detected premalignant lesions critically important its primary role initiation acceleration carcinogenesis. Thus, these potentially supplement immunohistochemical tests non-invasive detection adenoma. Moreover, become additional diagnostic prognostic tool development cancer. registered Australian New Zealand Clinical Trials Registry (ACTRN12624000976583) https://www.anzctr.org.au/.
Язык: Английский
Процитировано
1
Critical Reviews in Oncology/Hematology, Год журнала: 2024, Номер 203, С. 104480 - 104480
Опубликована: Авг. 22, 2024
Gastrointestinal (GI) cancer continues to pose a significant global health challenge. Recent advances in our understanding of the complex relationship between host and gut microbiota have shed light on critical role metabolic interactions pathogenesis progression GI cancer. In this study, we examined how interact with influence signalling pathways that impact formation tumours. Additionally, investigated potential therapeutic approach manipulating for use clinical settings. Revealing molecular exchanges facilitates deeper underlying mechanisms drive development. Metabolic hold promise identification microbial signatures or associated specific stages Hence, study provides strategies diagnosis, treatment management cancers improve patient outcomes.
Язык: Английский
Процитировано
5
Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)
Опубликована: Фев. 17, 2024
Adenomatous polyps (APs) with inflammation are risk factors for colorectal cancer. However, the role of inflammation-related gut microbiota in promoting progression APs is unknown.
Язык: Английский
Процитировано
4
Microbiology Spectrum, Год журнала: 2024, Номер 12(9)
Опубликована: Авг. 5, 2024
ABSTRACT Growing evidence have indicated the crucial role of intratumor microbiome in a variety solid tumor. However, intratumoral gynecological malignancies is largely unknown. In present study, total 90 Han patients, including 30 patients with cancer cervix, ovary, and endometrium each were enrolled, composition was assessed by 16S rDNA amplicon high throughput sequencing. We found that diversity metabolic potential all three types very similar. Furthermore, shared few taxa collectively take up relative abundance positive rate, Pseudomonas sp ., Comamonadaceae gen. Bradyrhizobium Saccharomonospora Cutibacterium acnes , Rubrobacter Dialister micraerophilus Escherichia coli . Additionally, Haemophilus parainfluenzae Paracoccus cervical cancer, Pelomonas ovarian Enterococcus faecalis endometrial identified LDA to be representative bacterial strain. addition, alpha-fetoprotein (AFP) (correlation coefficient = −0.3714) negatively correlated ( r 0.4, 95% CI: 0.03 0.7) CA199 0.3955) positively associated .. CA125 −0.4451) −0.4, −0.7 −0.09) Porphyromonas sp. CEA −0.3868) −0.02) This study promoted our understanding malignancies. IMPORTANCE this we compositional spectrum tumor microbes among similar sharing differentiated substantial species owned uniquely. Certain species, mostly unreported, clinical characteristics. prompted offered for affecting biology cancers female reproductive system.
Язык: Английский
Процитировано
4
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Янв. 30, 2025
Introduction Polygonatum kingianum is a well-known medicinal herb with proven bioactivities; however, little known about the effects of its polysaccharide on intestinal injuries in animals induced by lipopolysaccharide (LPS). Methods A total 30 Institute Cancer Research (ICR) mice were divided into control (CH), (MH), and treated (H) groups. Mice group H supplemented 100 mg/kg polysaccharides, while groups C M same amount normal saline gavage for 18 days. On th day LPS (10 mg/kg). Results The results showed weight MH significantly dropped ( P < 0.0001), PK had higher 0.01). Pathological analysis found that majority villi broken short, PK-treated longer considerably integrated villi. length CH 0.0001) was than M, value length/crypt depth smaller crypt 0.0001). Serum inspection MAD 0.05), IL-1β IL-6 TNF-α 0.01) MH, SOD 0.001), T-AOC 0.01), GSH-Px notably H. Microbiome sequencing obtained 844,477 raw 725,469 filtered reads. There 2,407 ASVs detected animals, there 312 328 shared between H, respectively. 5 phyla 20genera remarkable bacteria among including genera Escherichia, Pseudomonas_E, Mailhella, Paramuribaculum , NM07-P-09, Odoribacter, Nanosyncoccus SFM01, Onthenecus, Clostridium_Q UBA6985, Ructibacterium UBA946, Lachnoclostridium_B, Evtepia CAG-269, Limivicinus, Formimonas, Dehalobacterium, Dwaynesavagella UBA6985. We revealed could alleviate promoting oxidation resistance, decreasing inflammatory responses, accommodating microbiota mice. Discussion Our suggest possibility developing novel therapies diseases.
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Фев. 28, 2025
Colorectal polyps serve as the primary precursors for colorectal cancer. A close relationship has been observed between and gut microbiota. However, composition role of microbiome associated with tubular adenoma are not well understood. In this study, we prospectively evaluated alterations in microbiota among patients polyps. total 60 subjects were enrolled including 30 (CP group) healthy controls (control group). The 16S rRNA sequencing was employed to characterize fecal samples. results revealed that beta diversity CP group significantly differs from control (p = 0.001). At phylum level, relative abundance Bacteroides, Fusobacteria, Proteobacteria higher compared < 0.05), whereas Actinobacteria comparison 0.05). genus Bacteroides increased while Bifidobacterium declined species Clostridium perfringens, unidentified_Bacteroides, unidentified_Dorea, Escherichia coli, ramosum, Ruminococcus gnavus adolescentis, unclassified_Bifidobacterium, longum, Faecalibacterium prausnitzii, unidentified_Bifidobacterium is lower There a structural imbalance intestinal colonization flora patients, characterized by decrease beneficial bacteria an increase harmful bacteria. Escherichia, Shigella, may promising biomarkers early detection
Язык: Английский
Процитировано
0
European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177533 - 177533
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0