The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation DOI Creative Commons
Xinning Zeng, Jiaxuan Xin, Kaiyue Liu

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 252, С. 114637 - 114637

Опубликована: Фев. 10, 2023

Fungal contamination is omnipresent, and inhalation of fungi-contaminated organic dust leads to hypersensitivity pneumonitis (HP), in which neutrophils played a pivotal role. Existing studies have suggested that cell homeostasis crucial for the pathogenesis inflammatory disease. Although HMGB1 has been shown contribute suppressing HP, there lack on its mechanisms, especially regulation neutrophil homeostasis. This study aims investigate how regulates function by affecting homeostasis, then affects lung inflammation induced β-glucan, exposure marker fungi. Our results showed deficient led death disrupting balance between autophagy pyroptosis after β-glucan treatment. And deficiency exacerbated β-glucan-induced dysfunction both vivo vitro. Furthermore, contributed remodeling restricting aggravating exposure. funding could break towards cause during response, provides insights into HP potential biological targets The datasets used current are available from corresponding author reasonable request

Язык: Английский

Mitochondria-Targeting Type-I Photodrug: Harnessing Caspase-3 Activity for Pyroptotic Oncotherapy DOI
Zhigao Yi,

Xujuan Qin,

Li Zhang

и другие.

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(13), С. 9413 - 9421

Опубликована: Март 20, 2024

Precise control of cellular signaling events during programmed cell death is crucial yet challenging for cancer therapy. The modulation signal transduction in cells holds promise but limited by the lack efficient, biocompatible, and spatiotemporally controllable approaches. Here we report a photodynamic strategy that modulates both apoptotic pyroptotic altering caspase-3 protein activity associated crosstalk. This employs mitochondria-targeting, near-infrared activatable probe (termed M-TOP) functions via type-I photochemical mechanism. M-TOP less dependent on oxygen more effective treating drug-resistant cells, even under hypoxic conditions. Our study shows higher doses induce caspase-3/gasdermin-E pathway, whereas lower lead to apoptosis. method across diverse gasdermin-E-expressing cells. Moreover, mediated shift from can evoke controlled inflammatory response, leading robust balanced immune reaction. effectively inhibits distal tumor growth postsurgical recurrence. work demonstrates feasibility modulating intracellular through rational design anticancer drugs.

Язык: Английский

Процитировано

31
Cadmium toxicity and autophagy: a review DOI

Yueting Shao,

Liting Zheng,

Yiguo Jiang

и другие.

BioMetals, Год журнала: 2024, Номер 37(3), С. 609 - 629

Опубликована: Янв. 26, 2024

Язык: Английский

Процитировано

13
Role of Autophagy and Pyroptosis in Intervertebral Disc Degeneration DOI Creative Commons
Zhiqiang Wang, Xiaochun Li, Pengfei Yu

и другие.

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 91 - 100

Опубликована: Янв. 1, 2024

Abstract: Intervertebral disc degeneration is a chronic degenerative disease caused by the interaction of genetic and environmental factors, mainly manifested as lower back pain. At present, diagnosis intervertebral relies on imaging. However, early usually insidious, there currently lack relevant clinical biomarkers that can reliably reflect progression. Pyroptosis regulatory form cell death triggered activation inflammatory bodies caspase, which induce formation plasma membrane pores swelling or lysis. Previous studies have shown during progression degeneration, sustained inflammasomes leads to nuclear pyroptosis, occur in stages degeneration. Moreover, nucleus pulposus cells adapt external environment through autophagy maintain cellular homeostasis studying mechanism IDD intervening its pathological physiological processes provide new ideas for treatment IDD. This review analyzes effects pyroptosis reviewing literature recent years, order explore relationship between Keywords:

Язык: Английский

Процитировано

9
Berberine inhibits excessive autophagy and protects myocardium against ischemia/reperfusion injury via the RhoE/AMPK pathway DOI Creative Commons
Fajia Hu, Tie Hu, Yamei Qiao

и другие.

International Journal of Molecular Medicine, Год журнала: 2024, Номер 53(5)

Опубликована: Апрель 3, 2024

Several studies have shown that berberine (BBR) is effective in protecting against myocardial ischemia‑reperfusion injury (MI/RI). However, the precise molecular mechanism remains elusive. The present study observed and safeguarding effect of BBR hypoxia/reoxygenation (H/R) H9c2 cells. pretreatment significantly improved decrease cell viability, P62 protein, Rho Family GTPase 3 (RhoE) ubiquinone subunit B8 ubiquinol‑cytochrome c reductase core protein U, Bcl‑2‑associated X protein/B‑cell lymphoma 2 ratio, glutathione (GSH) GSH/glutathione disulphide (GSSG) ratio induced by H/R, while reducing increase lactate dehydrogenase, microtubule‑associated 1 light caspase‑3 activity, reactive oxygen species, GSSG malonaldehyde caused H/R. Transmission electron microscopy LysoTracker Red DND‑99 staining results showed inhibited H/R‑induced excessive autophagy mediating RhoE. also mitochondrial permeability transition, maintained stability membrane potential, reduced apoptotic rate, increased level caspase‑3. protective effects were attenuated pAD/RhoE‑small hairpin RNA, rapamycin (an activator) compound C AMP‑activated kinase inhibitor). These new findings suggested protects myocardium from MI/RI inhibiting autophagy, maintaining function, improving energy supply redox homeostasis, attenuating apoptosis through RhoE/AMP‑activated pathway.

Язык: Английский

Процитировано

9
Melatonin alleviates pyroptosis by regulating the SIRT3/FOXO3α/ROS axis and interacting with apoptosis in Atherosclerosis progression DOI Creative Commons
Cong Lin, Xiankun Liu,

Yiming Bai

и другие.

Biological Research, Год журнала: 2023, Номер 56(1)

Опубликована: Дек. 2, 2023

Atherosclerosis (AS), a significant contributor to cardiovascular disease (CVD), is steadily rising with the aging of global population. Pyroptosis and apoptosis, both caspase-mediated cell death mechanisms, play an essential role in occurrence progression AS. The human pineal gland primarily produces melatonin (MT), indoleamine hormone powerful anti-oxidative, anti-pyroptotic, anti-apoptotic properties. This study examined MT's anti-oxidative stress anti-pyroptotic effects on THP-1 macrophages treated nicotine.In vitro, were induced by 1 µM nicotine form pyroptosis model performed 30 mM MT for treatment. In vivo, ApoE-/- mice administered 0.1 mg/mL solution as drinking water, was intragastric administrated at 10 mg/kg/day. changes pyroptosis, oxidative detected.MT downregulated whose paralleled reduction reactive oxygen species (ROS) production, reversal sirtuin3 (SIRT3), Forkhead box O3 (FOXO3α) upregulation. also inhibited mainly caused interaction caspase-1 caspase-3 proteins. Vivo studies confirmed that could accelerate plaque formation. Moreover, showed AS lesion area.MT alleviates regulating SIRT3/FOXO3α/ROS axis interacting apoptosis. Importantly, our understanding inhibitory pathways macrophage will allow us identify other novel therapeutic targets help treat, prevent, reduce AS-associated mortality.

Язык: Английский

Процитировано

20
Autophagy in Its (Proper) Context: Molecular Basis, Biological Relevance, Pharmacological Modulation, and Lifestyle Medicine DOI Creative Commons
Miguel Á. Ortega, Oscar Fraile‐Martínez,

Diego De León-Oliva

и другие.

International Journal of Biological Sciences, Год журнала: 2024, Номер 20(7), С. 2532 - 2554

Опубликована: Янв. 1, 2024

Autophagy plays a critical role in maintaining cellular homeostasis and responding to various stress conditions by the degradation of intracellular components.In this narrative review, we provide comprehensive overview autophagy's molecular basis, biological significance, pharmacological modulation, its relevance lifestyle medicine.We delve into intricate mechanisms that govern autophagy, including macroautophagy, microautophagy chaperone-mediated autophagy.Moreover, highlight significance autophagy aging, immunity, metabolism, apoptosis, tissue differentiation systemic diseases, such as neurodegenerative or cardiovascular diseases cancer.We also discuss latest advancements modulation their potential implications clinical settings.Finally, explore intimate connection between factors emphasizing how nutrition, exercise, sleep patterns environmental can significantly impact autophagic process.The integration medicine research opens new avenues for promoting health longevity through personalized interventions.

Язык: Английский

Процитировано

6
Stellettin B Induces Cell Death in Bladder Cancer Via Activating the Autophagy/DAPK2/Apoptosis Signaling Cascade DOI Creative Commons

Chun-Han Chang,

Bo-Jyun Lin,

Chun‐Han Chen

и другие.

Marine Drugs, Год журнала: 2023, Номер 21(2), С. 73 - 73

Опубликована: Янв. 21, 2023

Bladder cancer (BC) is one of the most prevalent cancers worldwide. However, recurrence rate and five-year survival have not been significantly improved in advanced BC, new therapeutic strategies are urgently needed. The anticancer activity stellettin B (SP-2), a triterpene isolated from marine sponge Rhabdastrella sp., was evaluated with MTT assay as well PI Annexin V/7-AAD staining. Detailed mechanisms were elucidated through an NGS analysis, protein arrays, Western blotting. SP-2 suppressed viability BC cells without severe toxicity towards normal uroepithelial cells, it increased apoptosis activation caspase 3/8/9, PARP, γH2AX. phosphorylation FGFR3 its downstream targets downregulated by SP-2. Meanwhile, induced autophagy evidenced LC3-II formation p62 downregulation. inhibition using pharmacological inhibitors or ATG5-knockout protected RT-112 SP-2-induced cell suppression apoptosis. In addition, upregulation DAPK2 mRNA expression also contributed to cytotoxicity FGFR3-TACC3-knockout caused downregulation DAPK2, autophagy, conclusion, this first study demonstrating that exhibits potent anti-BC suppressing FGFR3-TACC3/Akt/mTOR pathway, which further activates novel autophagy/DAPK2/apoptosis signaling cascade.

Язык: Английский

Процитировано

15
The HIF-1/ BNIP3 pathway mediates mitophagy to inhibit the pyroptosis of fibroblast-like synoviocytes in rheumatoid arthritis DOI
Zhongyang Hong, Han Wang, Tianjing Zhang

и другие.

International Immunopharmacology, Год журнала: 2023, Номер 127, С. 111378 - 111378

Опубликована: Дек. 22, 2023

Язык: Английский

Процитировано

11
Programmed cell death, from liver Ischemia–Reperfusion injury perspective: An overview DOI Creative Commons

Shaobin Luo,

Rongkun Luo,

Gang Deng

и другие.

Heliyon, Год журнала: 2024, Номер 10(13), С. e32480 - e32480

Опубликована: Июнь 17, 2024

Liver ischemia-reperfusion injury (LIRI) can occur during liver resection, transplantation, shock, and other hemorrhagic conditions, resulting in profound local systemic effects via associated inflammatory responses hepatic cell death. Programmed death (PCD) occurs widely LIRI, different types of PCD play critical roles. Understanding the mechanisms underlying following LIRI is indispensable to mitigating widespread LIRI. Here, we review modalities their pertinence including mitochondrial permeability transition-dependent necrosis, apoptosis, necroptosis, ferroptosis, pyroptosis, autophagy, NETosis, parthanatos. We also evidence for role which may lead potential therapy.

Язык: Английский

Процитировано

4
Nicotinamide riboside alleviates sweeteners-induced brain and cognitive impairments in immature mice DOI
Yushan Jiang, Huaqi Zhang, Jing Shi

и другие.

Food & Function, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Nicotinamide riboside alleviated cognitive impairment induced by sucrose/aspartame, which might be related to neuroinflammation, pyroptosis, oxidative stress, apoptosis and autophagy.

Язык: Английский

Процитировано

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