Mechanisms of circRNA/lncRNA-miRNA interactions and applications in disease and drug research

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 162, С. 114672 - 114672

Опубликована: Апрель 13, 2023

In recent years, breakthroughs in bioinformatics have been made with the discovery of many functionally significant non-coding RNAs (ncRNAs). The these ncRNAs has further demonstrated multi-level characteristics intracellular gene expression regulation, which plays an important role assisting diagnosis, guiding clinical drug use and determining prognosis treatment process various diseases. microRNAs (miRNAs), long (lncRNAs), circular (circRNAs) are three major types that interact each other. Studies shown lncRNAs circRNAs can sponge miRNAs, thereby influencing normal physiological processes regulating mRNA and, thus, state cells. This paper summarizes mechanism action research progress ncRNA seven modalities. summary is intended to provide new ideas for diagnosing treating diseases researching developing drugs.

Язык: Английский

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New immune horizons in therapeutics and diagnostic approaches to Preeclampsia

American Journal of Reproductive Immunology, Год журнала: 2022, Номер 89(2)

Опубликована: Дек. 24, 2022

Abstract Hypertensive disorders of pregnancy (HDP) are one the commonest maladies, affecting 5%–10% pregnancies worldwide. The American College Obstetricians and Gynecologists (ACOG) identifies four categories HDP, namely gestational hypertension (GH), Preeclampsia (PE), chronic (CH), CH with superimposed PE. PE is a multisystem, heterogeneous disorder that encompasses 2%–8% all pregnancy‐related complications, contributing to about 9% 26% maternal deaths in low‐income countries 16% high‐income countries. These translate 50 000 over 500 fetal worldwide, therefore demanding high priority understanding clinical presentation, screening, diagnostic criteria, effective management. accompanied by uteroplacental insufficiency leading vascular metabolic changes, vasoconstriction, end‐organ ischemia. diagnosed after 20 weeks women who were previously normotensive or hypertensive. Besides shallow trophoblast invasion inadequate remodeling uterine arteries, dysregulation nonimmune system has been focal point This results from aberrant immune activation imbalanced differentiation T cells. Further, failure tolerance toward semi‐allogenic fetus due altered distribution Tregs such as CD4+FoxP3+ CD4+CD25+CD127(low) FoxP3+ cells, thereby creating cytotoxic environment suboptimal production immunosuppressive cytokines like IL‐10, IL‐4, IL‐13. Also, intracellular complement protein C5a may result decreased regulatory With dysfunction major driver pathogenesis, it logical therapeutic targeting components pharmacologic agents anti‐inflammatory immune‐modulating molecules either being used under trial. Cholesterol synthesis inhibitors Pravastatin improve placental perfusion PE, while Eculizumab (monoclonal antibody inhibiting C5) small molecular inhibitor C5a, Zilucoplan investigation. Monoclonal against IL‐17(Secukinumab) proposed alter Th imbalance Autologous Treg therapy checkpoint anti‐CTLA‐4 emerging new candidates horizons for management future.

Язык: Английский

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HUMAN IMMUNE SYSTEM: EXPLORING DIVERSITY ACROSS INDIVIDUALS AND POPULATIONS

Heliyon, Год журнала: 2025, Номер 11(2), С. e41836 - e41836

Опубликована: Янв. 1, 2025

The immune response is an intricate system that involves the complex connection of cellular and molecular components, each with distinct functional specialisations. It has a capacity to adjust mould in accordance specific stimuli, influenced by both genetic environmental factors. presence diversity, particularly across different ethnic racial groups, significantly contributes impact incidence diseases, disease susceptibility, autoimmune disorders, cancer risks regions certain populations. Environmental factors, including geography socioeconomic status, further modulate variety responses. These, turn, affect susceptibility infectious diseases development disorders. Despite complexity relationship, there remains gap understanding specificity indices races, reference ranges among populations, highlighting need for deeper diversity personalized approaches diagnostics therapeutics. This review systematically organizes these findings, goal emphasizing potential targeted interventions address health disparities advance translational research, enabling more comprehensive strategy. approach promises significant advancements identifying immunological conditions, focusing on interventions, through

Язык: Английский

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Epigenetic regulation of macrophage function in kidney disease: New perspective on the interaction between epigenetics and immune modulation

Biomedicine & Pharmacotherapy, Год журнала: 2025, Номер 183, С. 117842 - 117842

Опубликована: Янв. 13, 2025

Язык: Английский

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Advances in RNA therapy for the treatment of autoimmune diseases

Autoimmunity Reviews, Год журнала: 2025, Номер unknown, С. 103753 - 103753

Опубликована: Янв. 1, 2025

Язык: Английский

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Endogenous HClO-Gated Cascade MicroRNA Imaging for Precise Diagnosis of Atherosclerosis In Vivo

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Precise imaging of atherosclerotic plaques using biomarkers could prompt the diagnosis and clinical management atherosclerosis (AS)-driven cardiovascular diseases. MicroRNA-155 (miR-155) plays a critical role in AS development, with its expression notably upregulated foam cells within plaques. However, miRNA methods for face significant challenges, including low specificity, inefficient delivery, poor cell selectivity. Herein, we develop an endogenous hypochlorous acid (HClO)-gated cascade signal amplification strategy precise miR-155 living cells, enabling accurate vivo ex detection This utilizes phosphorothioate (PT)-modified hairpin probe that is specifically deprotected by HClO uncaged miR-155, triggering catalytic assembly (CHA) to amplify fluorescence signals. The PT-CHA probes are encapsulated lipid nanoparticles (LNs), followed conjugating phosphatidylserine (PS)-binding peptide (PBP) selectively targeting intensity PT-CHA@LN-PBP aorta region shows clear differentiation among AS-bearing mice, miR-155-/- healthy mice. Moreover, strongly correlates plaque area progression can discriminate vulnerability risk under curve (AUC) 0.94. Imaging human aortic tissues further validates probe's capacity distinguish from normal endarterium. These findings establish as noninvasive, reliable diagnostic tool assessment AS.

Язык: Английский

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MicroRNAs Have an Immunomodulatory Role in Diabetes Mellitus and Diabetic Cardiomyopathy

Cardiovascular Innovations and Applications, Год журнала: 2025, Номер 10(1)

Опубликована: Янв. 1, 2025

Diabetic cardiomyopathy (DCM), an independent diabetes complication, is characterized by abnormalities in myocardial structure, function, and metabolism, including diminished contractility, hypertrophy, fibrosis. Factors such as hyperglycemia, metabolic disorders, microangiopathy, inflammation, oxidative stress, insulin resistance have been found to play important roles DCM pathophysiology. Recent research has shown that miRNAs are involved processes cell proliferation, differentiation, metastasis, promote the progression of DCM. Despite these insights, regulatory effects on immune function remain inadequately explored. This review synthesizes current advancements understanding miRNA-mediated regulation We highlight need for further elucidate complex interactions between pathways DCM, which might reveal novel therapeutic targets mitigate this debilitating condition. Targeted B cells, macrophages, T cells through immune-associated open new avenues intervention. Developing efficient delivery systems miRNA-based therapies ensure targeted enhance stability. Additionally, combining existing hypoglycemic drugs with produce anti-inflammatory anti-fibrotic effects, thereby improving outcomes.

Язык: Английский

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Identification and Contribution of Inflammation-Induced Novel MicroRNA in the Pathogenesis of Systemic Lupus Erythematosus

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Апрель 4, 2022

Recently microRNAs (miRNAs) have been recognized as powerful regulators of many genes and pathways involved in the pathogenesis inflammatory diseases including Systemic Lupus Erythematosus (SLE). SLE is an autoimmune disease characterized by production various autoantibodies, immune cells, dysregulation epigenetic changes. Several candidate miRNAs regulating inflammation autoimmunity are described. In this study, we found significant increases expression miR21, miR25, miR186 peripheral blood mononuclear cells (PBMCs) patients compared to healthy controls. However, miR146a was significantly decreased controls negatively correlated with plasma estradiol levels activity scores (SLEDAI). We also that protein IL-12 IL-21 were increased Further, our data shows positively miR21 miR25 patients. addition, SLEDAI Thus, a dynamic interplay between miRNA expression. This study has translational potential may identify novel therapeutic targets SLE.

Язык: Английский

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Reduced miR-99a-3p levels in systemic lupus erythematosus may promote B cell proliferation via NCAPG and the PI3K/AKT signaling pathway

Lupus, Год журнала: 2024, Номер 33(4), С. 365 - 374

Опубликована: Фев. 6, 2024

Background Systemic lupus erythematosus is an immunologically dysregulated disease characterized by the presence of multiple autoantibodies. In SLE, B lymphocytes contribute to production autoantibodies and cytokines. Recently, we discovered that miR-99a-3p binds both EIF4EBP1 NCAPG mRNA lowering can promote cell autophagy in SLE increasing expression. However, functions have not been extensively investigated. Objective This work aims evaluate levels expression cells determine whether aberrant contributes pathological mechanisms SLE. Methods were obtained through immunomagnetic negative selection. Using RT-qPCR, expressions BALL-1 line measured. To relative abundance NCAPG, PI3K, p-PI3K, AKT, p-AKT, normalize them level β-actin using Western blotting. Evaluation NCAPG’s impact on proliferation was done utilizing CCK-8 assay. flow cytometry, cycle apoptosis Results Comparing healthy controls, significantly downregulated. Additionally, it observed had higher Blocking with antagomir elevated expression, facilitated PI3K/AKT pathway activation, improved proliferation, raised fraction S-phase cells, prevented apoptosis. The opposite effects upregulated agomir. Furthermore, effect decreased partially mediated elevating activating pathway. Conclusion Our research indicates lower appears boost number via pathways.

Язык: Английский

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UVB irradiation differential regulate miRNAs expression in skin photoaging

Anais Brasileiros de Dermatologia, Год журнала: 2022, Номер 97(4), С. 458 - 466

Опубликована: Май 31, 2022

UVB irradiation can cause acute damage such as sunburn, or photoaging and melanoma, all of which are major health threats.This study was designed to investigate the mechanism skin induced by radiation in mice through analysis differential expression miRNAs.A model constructed. HE Masson special stains were used examine modifications epidermis dermis mice. The miRNA profiles mouse exposed normal analyzed using miRNA-sequence analysis. GO Pathway employed for prediction targets.A total 23 miRNAs evaluated significantly different expressions comparison skin. Among them, 7 up-regulated 16 down-regulated with irradiation. is related a variety signal transduction pathways, among mmu-miR-195a-5p mitogen-activated protein kinase (MAPK) pathways crucial. There significant irradiation.Due time energy constraints, specific level verification, MAPK pathway exploration, miR-195a-5p downstream molecular need be further studied future.UVB-induced diagnosed treated miRNA.

Язык: Английский

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