B-Cell–Derived TGF-β1 Inhibits Osteogenesis and Contributes to Bone Loss in Periodontitis

Journal of Dental Research, Год журнала: 2023, Номер 102(7), С. 767 - 776

Опубликована: Апрель 21, 2023

B cells play a vital role in the elimination of periodontal pathogens, regulation immune response, and induction tissue destruction. However, dysfunction mesenchymal stem cell (MSC) differentiation to osteoblasts periodontitis (PD) has been poorly studied. Here we show that frequency CD45-CD105+CD73+ MSCs inflamed tissues is significantly decreased patients with PD compared healthy controls. CD19+ dominate infiltrated PD. Besides, B-cell depletion therapy reduces alveolar bone loss ligature-induced murine model. from mice express high level TGF-β1 inhibit osteoblast by upregulating p-Smad2/3 expression downregulating Runx2 expression. The inhibitory effect on reduced neutralization or Smad2/3 inhibitor. Importantly, B-cell-specific knockout increases number CD45-CD105+Sca1+ MSCs, ALP-positive activity, volume but decreases TRAP-positive osteoclast activity control littermates. Lastly, CD19+CD27+CD38- memory infiltrates both after initial therapy. Memory MSC osteoblasts. Thus, produced may contribute periodontitis, part, suppressing activity.

Язык: Английский

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Current Molecular, Cellular and Genetic Aspects of Peri-Implantitis Disease: A Narrative Review

Dentistry Journal, Год журнала: 2023, Номер 11(5), С. 134 - 134

Опубликована: Май 16, 2023

(1) Background: Peri-implantitis is a multi-factorial disease with an inflammatory background that occurs in both soft and hard tissues surrounding implants. In recent years, the understanding of cellular, molecular genetic peri-implantitis has broadened. This study aims to summarize currently available articles on subject highlight most advances over last 20 years. (2) Methods: For this study, Embase PubMed libraries were searched using keywords: (“peri-implantitis” AND “cytokine” OR “genetics” “cellular”) “cellular” “risk factors”). The search revealed total 3013 (992 from PubMed, 2021 Embase). Following screening titles abstracts full-text reads, 55 included. (3) Results: IL-6, IL-1β, TNF-α, MMP-8 their variations appear be important cytokines relation not only pathogenesis, but also potential diagnostic capabilities. Epithelial cells, along those bone lineage, are prime cellular elements found peri-implantitis. (4) Conclusions: A wide array cells stand behind peri-implantitis, as well take part process. However, growing interest topic led introduction specific new tools enable better patients’ responses treatment and, turn, even prediction risk developing peri-implant disease.

Язык: Английский

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Single-cell RNA sequencing combined with proteomics of infected macrophages reveals prothymosin-α as a target for treatment of apical periodontitis

Journal of Advanced Research, Год журнала: 2024, Номер 66, С. 349 - 361

Опубликована: Янв. 21, 2024

Chronic apical periodontitis (CAP) is a common infectious disease of the oral cavity. Immune responses and osteoclastogenesis monocytes/macrophages play crucial role in CAP progression, this study want to clarify CAP, which will contribute treatment CAP. We aim explore heterogeneity monocyte populations periapical lesion tissues healthy control (HC) periodontal by single-cell RNA sequencing (scRNA-seq), search novel targets for alleviating further validate it proteomics vitro vivo evaluations. ScRNA-seq was used analyze THP-1-derived macrophages with porphyromonas gingivalis infection were intersected differentially expressed genes (DEGs) between HC tissues. The upregulated PTMA (prothymosin-α) validated immunofluorescence staining quantitative real time polymerase chain reaction. evaluated effect Thymosin α1 (an amino-terminal proteolytic cleavage product protein) on inflammatory factors osteoclast differentiation infected P. gingivalis. Furthermore, we constructed mouse rat mandibular bone lesions caused periodontitis, estimated systemic topical administration Statistical analyses performed using GraphPad Prism software (v9.2) Monocytes divided into seven sub-clusters comprising monocyte-macrophage-osteoclast (MMO) 14 up-regulated 21 down-regulated proteins DEGs scRNA-seq data proteomics, including high expression PTMA. may decrease several cytokine expressions macrophages. Both mice pulp chamber rats alleviated lesions. upregulation moderates response prevents macrophages, provides basis targeted therapeutic strategies

Язык: Английский

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Tailoring Biomaterials Ameliorate Inflammatory Bone Loss

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(12)

Опубликована: Янв. 30, 2024

Abstract Inflammatory diseases, such as rheumatoid arthritis, periodontitis, chronic obstructive pulmonary disease, and celiac disrupt the delicate balance between bone resorption formation, leading to inflammatory loss. Conventional approaches tackle this issue encompass pharmaceutical interventions surgical procedures. Nevertheless, exhibit limited efficacy, while treatments impose trauma significant financial burden upon patients. Biomaterials show outstanding spatiotemporal controllability, possess a remarkable specific surface area, demonstrate exceptional reactivity. In present era, advancement of emerging biomaterials has bestowed more efficacious solutions for combatting detrimental consequences review, advances ameliorating loss are listed. Additionally, advantages disadvantages various biomaterials‐mediated strategies summarized. Finally, challenges perspectives analyzed. This review aims provide new possibilities developing advanced toward

Язык: Английский

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Fusobacterium nucleatum dysregulates inflammatory cytokines and NLRP3 inflammasomes in oral cells

Oral Diseases, Год журнала: 2024, Номер 30(7), С. 4767 - 4781

Опубликована: Фев. 26, 2024

This study aimed to clarify the difference in Fusobacterium nucleatum (F. nucleatum) induced inflammatory cytokines and nod-like receptor protein 3 (NLRP3) inflammasomes dysregulation among three periodontal cells.

Язык: Английский

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