Nanocarrier-mediated transdermal delivery of Lmidgf4 dsRNA expedites biological control of locusts by Beauveria bassiana

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 4, 2025

Locusts have been a major global agricultural pest that poses serious threat to crop and livestock production. Entomopathogenic fungi (EPF) provide an eco-friendly control method; however, their efficacy usually takes slow is unstable. To achieve enhancement of the biocontrol Beauveria bassiana (B. bassiana) against locusts, we developed new strategy by which B. nanocarrier-mediated dsRNA are co-applied across locust cuticle. The nanocarrier star polycation (SPc) effectively delivers Lmidgf4 (dsLmidgf4) into locust, targets Locusta migratoria imaginal disc growth factor 4 (Lmidgf4). SPc protects dsLmidgf4 from degradation hemolymph enables efficient gene silencing. Furthermore, has no adverse effects on spore germination growth. interference leads thinner layer endocuticle, thus facilitates infection bassiana, finally reduces median lethal time locusts infected with bassiana. In conclusion, combination dsRNA/SPc complex overcomes action fungi, providing novel for field locusts.

Язык: Английский

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Biology, classification, and entomopathogen-based management and their mode of action on Tuta absoluta (Meyrick) in Asia

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Авг. 7, 2024

, known as the South American tomato leaf miner, significantly impacts plants (

Язык: Английский

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Entomopathogenic fungi promising biocontrol agents for managing lepidopteran pests: Review of current knowledge

Biocatalysis and Agricultural Biotechnology, Год журнала: 2024, Номер 58, С. 103146 - 103146

Опубликована: Апрель 3, 2024

Язык: Английский

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Visualizing Oral Infection Dynamics of Beauveria bassiana in the Gut of Tribolium castaneum

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 101 - 101

Опубликована: Янв. 28, 2025

The ability of entomopathogenic fungi, such as Beauveria bassiana, to infect insects by penetrating their cuticle is well documented. However, some have evolved mechanisms combat fungal infections. red flour beetle (Tribolium castaneum), a major pest causing significant economic losses in stored product environments globally, embeds antifungal compounds within its protective barrier. Previous reports addressed the contributions non-cuticular infection routes, noting an increase mortality beetles fed with conidia. In this study, we further explore progression and dynamics oral exposure gut T. castaneum after feeding encapsulated B. bassiana conidia formulation. First, characterized formulation surface using atomic force microscopy, observing no topological differences between capsules containing not Confocal microscopy confirmed uniform distribution hydrogel matrix. Then, larvae adult conidia-encapsulated exhibited distributed throughout alimentary canal, higher presence before pyloric chamber. More were found larval midgut hindgut compared adults, but germinated observed epithelium. These results suggest that obstructs gut, impairing insect's ingest, process, absorb nutrients. This disruption may weaken host, increasing susceptibility infections and, ultimately, leading death. By providing first direct observation canal castaneum, study highlights novel aspect fungal-host interaction opens new avenues for advancing fungal-based control strategies exploiting stage-specific vulnerabilities.

Язык: Английский

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The fungal protease BbAorsin contributes to growth, conidiation, germination, virulence, and antiphytopathogenic activities in Beauveria bassiana (Hypocreales: Cordycipitaceae)

Pesticide Biochemistry and Physiology, Год журнала: 2024, Номер 202, С. 105936 - 105936

Опубликована: Апрель 29, 2024

Язык: Английский

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Uncovering the immune evasion mechanism of microbial pathogens: lessons from anti‐encapsulation strategies adopted by a fungus

Integrative Zoology, Год журнала: 2024, Номер 19(6), С. 1229 - 1232

Опубликована: Июнь 28, 2024

Overview of Hemocytin-Mediated Cellular Encapsulation and Melanization Responses in Insects Against Fungal Pathogens.

Язык: Английский

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N-acetylglucosamine kinase (BbHxk1) has pleiotropic effects on vegetative growth, cell wall integrity, morphological transition, cuticle infection, and metabolic modulation in the biological pesticide Beauveria bassiana

Pesticide Biochemistry and Physiology, Год журнала: 2024, Номер 203, С. 106015 - 106015

Опубликована: Июль 3, 2024

Язык: Английский

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Role of cuticular genes in the insect antimicrobial immune response

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Июль 23, 2024

Insects are established models for understanding host-pathogen interactions and innate immune mechanisms. The system in insects is highly efficient recognizing opposing pathogens that cause detrimental effects during infection. cuticular layer which covers the superficial of insect body participates host defense wound healing by inducing responses. Previous studies have started to address involvement genes conferring resistance pathogens, particularly those infect disrupting cuticle. For example, gene Transglutaminase (TG) Drosophila melanogaster plays a structural role cuticle formation blood coagulation also possesses properties against pathogenic However, more information becoming available about function other families insects. In this review, we aim highlight recent advances immunity necessity pursuing further research fill existing gaps important field immunology. This will lead novel strategies management agricultural pests vectors plant human disease.

Язык: Английский

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Opportunities for optimizing fungal biological control agents for long-term and effective management of insect pests of orchards and vineyards: a review

Frontiers in Fungal Biology, Год журнала: 2024, Номер 5

Опубликована: Авг. 1, 2024

Novel tactics for controlling insect pests in perennial fruit and nut crops are needed because target often display decreased susceptibility to chemical controls due overreliance on a handful of active ingredients regulatory issues. As an alternative controls, entomopathogenic fungi could be utilized as biological control agents manage pest populations. However, development field ready products is hampered by lack basic knowledge. Development requires collecting, screening, characterizing greater variety potential fungal species strains. Creation standardized research framework study will aid identifying the mechanisms activity that possess, including antibiotic metabolite production; strains best suited survive different climates agroecosystems; optimized combinations novel formulations. This mini review therefore discusses strategies collect characterize new strains, test biocontrol activity, examine ability tolerate climates, lastly how utilize this information develop into growers.

Язык: Английский

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Functional characterization and regulatory pattern of Neoseiulus barkeri peptidoglycan recognition protein (PGRP)

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 293, С. 139458 - 139458

Опубликована: Янв. 2, 2025

Язык: Английский

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