Direct Analysis of Marine Dissolved Organic Matter Using LC-FT-ICR MS

Environmental Science & Technology, Год журнала: 2024, Номер 58(10), С. 4637 - 4647

Опубликована: Март 1, 2024

Marine dissolved organic matter (DOM) is an important component of the global carbon cycle, yet its intricate composition and sea salt matrix pose major challenges for chemical analysis. We introduce a direct injection, reversed-phase liquid chromatography ultrahigh resolution mass spectrometry approach to analyze marine DOM without need solid-phase extraction. Effective separation achieved with large chromatographic column extended isocratic aqueous step. Postcolumn dilution sample flow buffer-free solvents implementing counter gradient reduced buildup in ion source resulted excellent repeatability. With this method, over 5,500 unique molecular formulas were detected from just 5.5 nmol 100 μL filtered Arctic Ocean seawater. observed highly linear detector response variable concentrations high robustness against matrix. Compared extracted DOM, our injection method demonstrated superior sensitivity heteroatom-containing DOM. The analysis seawater offers fast simple preparation avoids fractionation introduced by facilitates studies environments, where only minimal volume available e.g. sediment pore water, ice cores, or permafrost soil solution. small requirement also supports higher spatial (e.g., soils) temporal culture experiments). Chromatographic adds further information formulas, enhancing understanding biogeochemistry, chemodiversity, ecological processes.

Язык: Английский

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Dissolved organic carbon leaching from microplastics and bioavailability in coastal ecosystems

The Science of The Total Environment, Год журнала: 2023, Номер 909, С. 168673 - 168673

Опубликована: Ноя. 18, 2023

The dissolved organic carbon (DOC) leached from two types of microplastics (polyethylene and polypropylene) frequently found in coastal areas were evaluated situ. Subsequently, the bioavailability DOC was assessed for microbial inocula different communities (i.e., estuarine open-coastal waters, river-mouth waters seagrass beds). Leached largely biodegradable (as much as 85 %). However, beds exhibited lower utilization efficiency than probably because differences their communities. labile/recalcitrant ratio plastic similar under illuminated dark conditions, whereas polyethylene, rather polypropylene, preferentially used by We estimated that many 21,000 metric tons plastics may be released into ocean annually. Our results support need to consider potential impacts pollution on communities, including consideration trophic webs cycle.

Язык: Английский

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A global database of dissolved organic matter (DOM) concentration measurements in coastal waters (CoastDOM v1)

Earth system science data, Год журнала: 2024, Номер 16(2), С. 1107 - 1119

Опубликована: Фев. 27, 2024

Abstract. Measurements of dissolved organic carbon (DOC), nitrogen (DON), and phosphorus (DOP) concentrations are used to characterize the matter (DOM) pool important components biogeochemical cycling in coastal ocean. Here, we present first edition a global database (CoastDOM v1; available at https://doi.org/10.1594/PANGAEA.964012, Lønborg et al., 2023) compiling previously published unpublished measurements DOC, DON, DOP waters. These data complemented by hydrographic such as temperature salinity and, extent possible, other variables (e.g. chlorophyll a, inorganic nutrients) system total alkalinity). Overall, CoastDOM v1 includes observations from all continents. However, most were collected Northern Hemisphere, with clear gap DOM Southern Hemisphere. The included 1978 2022 consist 62 338 points for 20 356 13 533 DOP. number decreases progressively sequence DOC > DON DOP, reflecting both differences maturity analytical methods greater focus on aquatic science community. shows that average concentration waters (average ± standard deviation (SD): 182±314 µmol C L−1; median: 103 L−1) is 13-fold higher than (13.6±30.4 N 8.0 L−1), which itself 39-fold (0.34±1.11 P 0.18 L−1). This dataset will be useful identifying spatial temporal patterns help facilitate reuse studies aimed better characterizing local processes; closing nutrient budgets; estimating carbon, nitrogen, phosphorous pools; establishing baseline modelling future changes

Язык: Английский

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Relating Molecular Properties to the Persistence of Marine Dissolved Organic Matter with Liquid Chromatography–Ultrahigh-Resolution Mass Spectrometry

Environmental Science & Technology, Год журнала: 2024, Номер unknown

Опубликована: Фев. 9, 2024

Marine dissolved organic matter (DOM) contains a complex mixture of small molecules that eludes rapid biological degradation. Spatial and temporal variations in the abundance DOM reflect existence fractions are removed from ocean over different time scales, ranging seconds to millennia. However, it remains unknown whether intrinsic chemical properties these components relate their persistence. Here, we elucidate compare molecular compositions distinct with lability along water column North Atlantic Gyre. Our analysis utilized ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry at 21 T coupled liquid chromatography novel data pipeline developed CoreMS generates formula assignments metrics isomeric complexity. Clustering binned 14 857 into groups correspond depth distribution semilabile, semirefractory, refractory DOM. The more labile were concentrated near surface contained aliphatic, hydrophobic, reduced than fraction, which occurred uniformly throughout column. These findings suggest processes selectively remove hydrophobic compounds, such as aggregation particle sorption, contribute variable removal rates marine

Язык: Английский

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Toxicity of rare earth elements (REEs) to marine organisms: Using species sensitivity distributions to establish water quality guidelines for protecting marine life

Environmental Research, Год журнала: 2024, Номер 261, С. 119708 - 119708

Опубликована: Июль 30, 2024

Язык: Английский

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Microbial functional diversity and redundancy: moving forward

FEMS Microbiology Reviews, Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract Microbial functional ecology is expanding as we can now measure the traits of wild microbes that affect ecosystem functioning. Here, review techniques and advances could be bedrock for a unified framework to study microbial functions. These include our newfound access environmental genomes, collections traits, but also ability microbes’ distribution expression. We then explore technical, ecological, evolutionary processes explain patterns diversity redundancy. Next, suggest reconciling microbiology with biodiversity-ecosystem-functioning studies by experimentally testing significance redundancy efficiency, resistance, resilience processes. Such will aid in identifying state shifts tipping points microbiomes, enhancing understanding how where microbiomes guide Earth's biomes context changing planet.

Язык: Английский

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DOM production, removal, and transformation processes in marine systems

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 137 - 246

Опубликована: Янв. 1, 2024

Язык: Английский

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Influence of amino acids on bacterioplankton production, biomass and community composition at Ocean Station Papa in the subarctic Pacific

Elementa Science of the Anthropocene, Год журнала: 2023, Номер 11(1)

Опубликована: Янв. 1, 2023

Bacterioplankton play a central role in carbon cycling, yet their relative contributions to production and removal can be difficult constrain. As part of the Export Processes Ocean from RemoTe Sensing (EXPORTS) program, this study identifies potential influences bacterioplankton community dissolved organic matter (DOM) composition on cycling at Station Papa August 2018. Surface (5–35 m) rates stocks spanned 2- 3-fold range over 3-week cruise correlated positively with DOM degradation state, estimated using mole proportion total amino acids. When was more degraded, 16S rRNA gene amplicon data revealed less diverse significant contribution members Flavobacteriaceae family. Over course 7–10 d, as quality improved (became degraded) productivity increased, responding became diverse, increased SAR86, SAR11 AEGEAN-169 clades. The mean for mixed layer, depth-integrated demand (gross production) 5.2 mmol C m−2 d−1, representing 60% net primary production, where difference between than sinking flux 50 m. concentrations (cruise average 58.5 µM C) did not exhibit systematic change period. Therefore, we hypothesize that supplied gross values were greater provided necessary account excess production. These findings highlight Papa, site high recycling.

Язык: Английский

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A global database of dissolved organic matter (DOM) measurements in coastal waters (CoastDOM v1)

Опубликована: Сен. 27, 2023

Abstract. The measurements of dissolved organic carbon (DOC), nitrogen (DON), and phosphorus (DOP) are used to characterize the matter (DOM) pool important components biogeochemical cycling in coastal ocean. Here, we present first edition a global database (CoastDOM v1; available at https://figshare.com/s/512289eb43c4f8e8eaef) compiling previously published unpublished DOC, DON, DOP collected waters. These data complemented by hydrographic such as temperature salinity and, extent possible, other variables (e.g., Chlorophyll-a, inorganic nutrients) system total alkalinity). Overall, CoastDOM v1 includes observations from all continents however, most were Northern Hemisphere, with clear gap water DOM Southern Hemisphere. included 1978 2022 consist 62339 points for 20360 DON 13440 DOP. number decreases progressively sequence DOC > DOP, reflecting both differences maturity analytical methods greater focus on aquatic science community. shows that average concentration waters (average (standard deviation; SD): 182 (314) µmol C L−1; median: 103 L−1), is 13-fold than concentrations (SD): 13.6 (30.4) N 8.0 which was itself 39-fold 0.34 ± 1.11 P 0.18 L−1). This dataset will be useful identify spatial temporal patterns facilitate reuse studies aimed better characterising local processes, closing nutrient budgets, estimating carbon, phosphorous pools, well identifying baseline modelling future changes

Язык: Английский

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Microbial carbon oxidation in seawater below the hypoxic threshold

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 22, 2025

Global oxygen minimum zones (OMZs) often reach hypoxia but seldom anoxia. Recently it was reported that Michaelis Menten constants (Km) of oxidative enzymes are orders magnitude higher than respiratory Km values, and in the Hypoxic Barrier Hypothesis proposed that, ecosystems experiencing falling oxygen, oxygenase enzyme activities become oxygen-limited long before respiration. We conducted a mesocosm experiment with phytoplankton bloom as an organic carbon source controlled dissolved (DO) concentrations dark to determine whether slows oxidation decline. Total utilization (TOU) hypoxic treatment (ca. 7.1 µM O2) 21.7% lower oxic 245.1 over first 43 days experiment. In addition, following restoration fully conditions treatment, TOU accelerated, demonstrating processes sensitive DO found large volumes ocean. Microbial amplicon-based community composition diverged between treatments, indicating specialized microbiome included Thioglobaceae (SUP05 Gammaproteobacteria), OM190 (Planctomycetota), ABY1 (Patescibacteria), SAR86 subclade D2472, thrived while genus Candidatus Actinomarina SAR11 alphaproteobacteria were sharply inhibited. Our findings support hypothesis kinetics might slow progression ocean deoxygenation oxygen-poor regions be factor evolution microbial taxa adapted environments.

Язык: Английский

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