Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Microorganisms, Год журнала: 2022, Номер 10(4), С. 753 - 753

Опубликована: Март 30, 2022

Filamentous fungi are a large and diverse taxonomically group of microorganisms found in all habitats worldwide. They grow as network cells called hyphae. Since filamentous live very habitats, they produce different enzymes to degrade material for their living, example hydrolytic various kinds biomasses. Moreover, defense proteins (antimicrobial peptides) attaching surfaces (hydrophobins). Many them easy cultivate known setups (submerged fermentation solid-state fermentation) secretion often much larger than what is seen from yeast bacteria. Therefore, many industries the preferred production hosts enzymes. Edible have traditionally been used food, such mushrooms or fermented foods. New trends use edible myco-protein enriched This review gives an overview proteins, enzymes, peptides produced by most well-known cell factories purposes applications. we describe some challenges that important consider when optimized efficient factories.

Язык: Английский

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Microbial cellulase production and its potential application for textile industries

Annals of Microbiology, Год журнала: 2023, Номер 73(1)

Опубликована: Апрель 10, 2023

Abstract Purpose The textile industry’s previous chemical use resulted in thousands of practical particulate emissions, such as machine component damage and drainage system blockage, both which have implications. Enzyme-based processing is cost-effective, environmentally friendly, non-hazardous, water-saving. purpose this review to give evidence on the potential activity microbial cellulase industry, mostly confined realm research. Methods This was progressive by considering peer-reviewed papers linked production, its prospective application for industries appraised produced develop assessment. Articles were divided into two categories based results trustworthy educational journals: methods used produce diversity microorganisms through fermentation processes approaches microbes fermentation. Submerged (SMF) solid-state (SSF) techniques are currently being meet industrial demand production bio industry. Results Microbial vital increasing day due no side effect environment human health becoming increasingly important. In conventional processing, gray cloth subjected a series treatments that involved breaking dye molecule’s amino group with Cl − , started accelerated dye(-resistant) bond cracking. A enzyme primarily derived from variety species found various ecological settings biotextile/bio-based product technology future needs applications. Conclusion Cellulase has been advantages cellulose-based textiles, well quality enhancement fabric maintenance over traditional approaches. Cellulase’s role industry chosen an appropriate sound solution long healthy lifestyle.

Язык: Английский

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From fungal secretomes to enzymes cocktails: The path forward to bioeconomy

Biotechnology Advances, Год журнала: 2021, Номер 52, С. 107833 - 107833

Опубликована: Сен. 3, 2021

Язык: Английский

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Technological advances for improving fungal cellulase production from fruit wastes for bioenergy application: A review

Environmental Pollution, Год журнала: 2021, Номер 287, С. 117370 - 117370

Опубликована: Май 15, 2021

Язык: Английский

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Encapsulation of Trichoderma harzianum with nanocellulose/carboxymethyl cellulose nanocomposite

Carbohydrate Polymers, Год журнала: 2022, Номер 295, С. 119876 - 119876

Опубликована: Июль 19, 2022

Язык: Английский

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Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites

Journal of King Saud University - Science, Год журнала: 2021, Номер 34(1), С. 101695 - 101695

Опубликована: Ноя. 9, 2021

The cellulase enzyme is used for various industrial applications such as textile, paper, food and biofuel industries. Industrially, the fungal strain, Aspergillus niger, widely to produce enzymes. Emerging evidence has indicated possible role of magnetic nanocomposites in enhancing production by A. niger. cellulase/iron oxide are already reported be an eco-friendly method modulating microbial biochemical characteristics. present study was assess efficacy composites (CMNPS) enhanced enzyme. strain niger isolated from soil samples using standard techniques. fungi were then transfected with CMNPS further evaluated Synthesis characterized UV, FTIR, XRD. produced substrate production. analyzed CMC (0.82 IU/ml activity) FPA assay (0.039 activity). also (0.74 Reuse ultimate application ecofriendly approach synthesis concludes that cellulase/ iron may a useful tool fungus

Язык: Английский

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Microbial cellulase production using fruit wastes and its applications in biofuels production

International Journal of Food Microbiology, Год журнала: 2022, Номер 378, С. 109814 - 109814

Опубликована: Июнь 16, 2022

Язык: Английский

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Degradation potential of different lignocellulosic residues by Trichoderma longibrachiatum and Trichoderma afroharzianum under solid state fermentation

Process Biochemistry, Год журнала: 2021, Номер 112, С. 6 - 17

Опубликована: Ноя. 10, 2021

Язык: Английский

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Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Biotechnology for Biofuels, Год журнала: 2021, Номер 14(1)

Опубликована: Ноя. 22, 2021

Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the fungi obtained natural breeding have low secretory capacity cellulase production, are far from meeting requirements industrial production. Random mutagenesis combined with adaptive laboratory evolution (ALE) strategy an effective method increase production fungal enzymes. This study mutant Trichoderma afroharzianum exposures N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), Ethyl Methanesulfonate (EMS), Atmospheric Room Temperature Plasma (ARTP) ALE high stress. The T. MEA-12 0.60, 5.47, 0.31 2.17 IU/mL FPase, CMCase, pNPCase pNPGase, respectively. These levels were 4.33, 6.37, 4.92 4.15 times higher than those parental strain, Also, it was found that had same carbon catabolite repression (CCR) effect as other liquid submerged fermentation. In contrast, can tolerate inhibition glucose (up 20 mM) without affecting enzyme under inducing conditions. Interestingly, crude showed enzymatic hydrolysis efficiency against three different biomasses (cornstalk, bamboo reed), when reesei Rut-C30. addition, factors improved clarified. Overall, compound effectively increased cellulase. A super-producing obtained, its could hydrolyze common efficiently, combination enzymes derived model strain reesei, provides new choice for processing bioresources future.

Язык: Английский

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Integrated Genomics and Transcriptomics Provide Insights into Salt Stress Response in Bacillus subtilis ACP81 from Moso Bamboo Shoot (Phyllostachys praecox) Processing Waste

Microorganisms, Год журнала: 2024, Номер 12(2), С. 285 - 285

Опубликована: Янв. 29, 2024

Salt stress is detrimental to the survival of microorganisms, and only a few bacterial species produce hydrolytic enzymes. In this study, we investigated expression salt stress-related genes in salt-tolerant strain Bacillus subtilis ACP81, isolated from bamboo shoot processing waste, at transcription level. The results indicate that could grow 20% NaCl, sub-lethal concentration was 6% NaCl. Less neutral protease higher cellulase β-amylase activities were observed for B. ACP81 under concentrations than control (0% NaCl). Transcriptome analysis showed adapted high-salt conditions by upregulating involved cellular processes (membrane synthesis) defense systems (flagellar assembly, compatible solute transport, glucose metabolism, phosphotransferase system). Interestingly, encoding β-amylase-related (malL, celB, celC) significantly upregulated starch sucrose metabolic pathways, accumulated effective mitigating stress. RT-qPCR performed confirm sequencing data. This study emphasizes that, conditions, exhibits enhanced activities, providing an important germplasm resource saline soil reclamation enzyme development.

Язык: Английский

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