Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens

Poultry Science, Год журнала: 2022, Номер 102(3), С. 102433 - 102433

Опубликована: Дек. 16, 2022

Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds. In this study, we investigated the of quercetin supplementation inflammatory responses, barrier microbial community LPS-challenged laying hens. A total two hundred eighty-eight 32-wk-old Jingfen No.6 hens were randomly assigned 3 groups, CON group, LC group LQ group. was fed with 0.4 mg/kg at end 12 wk, groups challenged intraperitoneally lipopolysaccharide (LPS). After LPS challenge, 8 birds each selected sampled. challenge induced an obvious mucosal injury, necrosis shedding, while intervention maintained its structure. Quercetin significantly decreased elevated malondialdehyde contents (P < 0.05), increased activity antioxidant capacity glutathione peroxidase 0.05) mucosa rescued LPS-induced decreases goblet cell density mucin2 expression levels 0.05). There significant decline mRNA Claudin1 Occludin layers, which could be alleviated by dietary quercetin. IL-1β TLR-4 mucosa, these rises reversed following supplementation. shift microenvironment, addition elevate relative abundance some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, Bacteroides_salanitronis. conclusion, ameliorated challenge-induced inflammation improved functions, possibly associated modulation microbiota, particularly population SCFA-producing bacteria.

Язык: Английский

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Targeting the Gut–Eye Axis: An Emerging Strategy to Face Ocular Diseases

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(17), С. 13338 - 13338

Опубликована: Авг. 28, 2023

The human microbiota refers to a large variety of microorganisms (bacteria, viruses, and fungi) that live in different body sites, including the gut, oral cavity, skin, eyes. In particular, presence an ocular surface with crucial role maintaining homeostasis by preventing colonization from pathogen species has been recently demonstrated. Moreover, recent studies underline potential association between gut (GM) health. this respect, some evidence supports existence gut-eye axis involved pathogenesis several diseases, age-related macular degeneration, uveitis, diabetic retinopathy, dry eye, glaucoma. Therefore, understanding link GM these disorders might be useful for development new therapeutic approaches, such as probiotics, prebiotics, symbiotics, or faecal transplantation through which could modulated, thus allowing better management diseases.

Язык: Английский

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Dysfunction of cecal microbiota and CutC activity in mice mediating diarrhea with kidney-yang deficiency syndrome

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 4, 2024

Objective Previous studies have indicated that diarrhea with kidney-yang deficiency syndrome leads to a disorder of small intestine contents and mucosal microbiota. However, the relationship TMA-lyase (CutC) activity TMAO remains unexplored. Therefore, this study explores between cecal microbiota choline activity, as well correlation trimethylamine oxide (TMAO), inflammatory index, CutC activity. Method Twenty SPF-grade male KM mice were randomly divided into normal group (CN) model (CD). Diarrhea mouse models established by adenine combined Folium sennae administration. TMAO, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) levels detected, content was sequenced. Result After 14 days, occurred in CD group. Compared CN group, there no significant change while cecum significantly increased, IL-6, TNF-α showed increase. The Chao1 Observed_species Shannon Simpson index all exhibited decreasing trend. main changes at bacterial genus level Alistipes , Enterorhabdus Desulfovibrio Bacteroides Candidatus_Saccharimonas [Ruminococcus]_torques_group . results LEfSe analysis, random forest analysis ROC curve revealed Paludicola Blautia Negativibacillus Paraprevotella Harryflintia Candidatus_Soleaferrea Anaerotruncus Oscillibacter Colidextribacter characteristic bacteria Correlation negative Ligilactobacillus positive positively correlated IL-6. Conclusion affects physiological status, digestive enzyme levels, response mice. Additionally, are composition function microbiota, indicating an important impact on host intestinal balance. occurrence may be associated dysbiosis increased elevated heightened factor levels.

Язык: Английский

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Gut Microbiota Profiles in Myopes and Nonmyopes

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(5), С. 2 - 2

Опубликована: Май 1, 2024

To identify compositional differences in the gut microbiome of nonmyopes (NM) and myopes using 16S ribosomal RNA sequencing to investigate whether may contribute onset or progression condition.

Язык: Английский

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Diabetes and gut microbiome

Frontiers in Microbiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

Diabetes mellitus represents a significant global health problem. The number of people suffering from this metabolic disease is constantly rising and although the incidence heterogeneous depending on region, country, economic situation, lifestyle, diet level medical care, it increasing worldwide, especially among youths children, mainly due to lifestyle environmental changes. pathogenesis two most common subtypes diabetes mellitus, type 1 (T1DM) 2 (T2DM), substantially different, so each form characterized by different causation, etiology, pathophysiology, presentation, treatment. Research in recent decades increasingly indicates potential role gut microbiome initiation, development, progression disease. Intestinal microbes their fermentation products have an important impact host metabolism, immune system, nutrient digestion absorption, barrier integrity protection against pathogens. This review summarizes current evidence changes microbial populations both types mellitus. Attention focused abundance specific bacterial groups at taxonomic levels humans, shift also assessed relation geographic location, age, antidiabetic drug. causal relationship between bacteria still unclear, future studies applying new methodological approaches broader range microorganisms inhabiting digestive tract are urgently needed. would not only provide better understanding disease, but use beneficial species probiotics for treatment diabetes.

Язык: Английский

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Investigating the effects of rare ginsenosides on hyperuricemia and associated sperm damage via nontargeted metabolomics and gut microbiota

Journal of Ethnopharmacology, Год журнала: 2024, Номер 332, С. 118362 - 118362

Опубликована: Май 18, 2024

Язык: Английский

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The ideal treatment timing for diabetic retinopathy: the molecular pathological mechanisms underlying early-stage diabetic retinopathy are a matter of concern

Frontiers in Endocrinology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 9, 2023

Diabetic retinopathy (DR) is a prevalent complication of diabetes, significantly impacting patients’ quality life due to vision loss. No pharmacological therapies are currently approved for DR, excepted the drugs treat diabetic macular edema such as anti-VEGF agents or steroids administered by intraocular route. Advancements in research have highlighted crucial role early intervention DR halting delaying disease progression. This holds immense significance enhancing and alleviating societal burden associated with medical care costs. The non-proliferative stage represents phase DR. In comparison proliferative stage, pathological changes primarily manifest microangiomas hemorrhages, while at cellular level, there loss pericytes, neuronal cell death, disruption components functionality within retinal vascular unit encompassing pericytes neurons. Both neurodegenerative microvascular abnormalities stages Therefore, our focus lies on we initially summarized mechanisms involved its development, including pathways polyols, that revolve around occurring during this stage. We also integrate cutting-edge mechanisms, leukocyte adhesion, neutrophil extracellular traps, multiple RNA regulation, microorganisms, death (ferroptosis pyroptosis), other related mechanisms. current status drug therapy early-stage discussed provide insights development pharmaceutical interventions targeting treatment

Язык: Английский

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Gut Microbiota Patterns in Patients with Non-Alcoholic Fatty Liver Disease: A Comprehensive Assessment Using Three Analysis Methods

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(20), С. 15272 - 15272

Опубликована: Окт. 17, 2023

Non-alcoholic fatty liver disease (NAFLD) is considered the most common chronic worldwide, affecting nearly 25% of global adult population. Increasing evidence suggests that functional and compositional changes in gut microbiota may contribute to development promote progression NAFLD. 16S rRNA gene next-generation sequencing widely used determine specific features NAFLD microbiome, but a complex system such as requires comprehensive approach. We three different approaches: MALDI-TOF-MS bacterial cultures, qPCR, NGS sequencing, well wide variety statistical methods assess differences composition between patients without significant fibrosis control group. The listed showed enrichment Collinsella sp. Oscillospiraceae for samples Lachnospiraceae (and particular Dorea sp.) Veillonellaceae families, Bifidobacteriaceae, Lactobacillaceae, Enterococcaceae (particularly Enterococcus faecium faecalis), were also found be important taxa microbiome evaluation. Considering individual method observations, an increase Candida krusei decrease Bacteroides uniformis detected using MALDI-TOF-MS. An Gracilibacteraceae, Chitinophagaceae, Pirellulaceae, Erysipelatoclostridiaceae, Muribaculaceae, Comamonadaceae, Acidaminococcaceae observed with NGS, Fusobacterium nucleatum was shown qPCR analysis. These findings confirm associated composition. Further investigations are required cause-and-effect relationships impact microbiota-derived compounds on

Язык: Английский

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Gastrodin alleviates the deterioration of depressive-like behavior and glucolipid metabolism promoted by chronic stress in type 2 diabetic mice

European Journal of Pharmacology, Год журнала: 2024, Номер 973, С. 176582 - 176582

Опубликована: Апрель 19, 2024

Язык: Английский

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Gut Microbiota Dysbiosis in Diabetic Retinopathy—Current Knowledge and Future Therapeutic Targets

Life, Год журнала: 2023, Номер 13(4), С. 968 - 968

Опубликована: Апрель 7, 2023

Diabetic retinopathy is one of the major causes blindness today, despite important achievements in diagnosis and therapy. The involvement a gut–retina axis thought to be possible risk factor for several chronic eye disease, such as glaucoma, age-related macular degeneration, uveitis, and, recently, diabetic retinopathy. Dysbiosis may cause endothelial disfunction alter retinal metabolism. This review analyzes evidence regarding changes gut microbiota patients with DR compared diabetics healthy controls (HCs). A systematic was performed on PubMed, Web Science, Google Scholar following terms: “gut microbiota” OR microbiome” AND “diabetic retinopathy”. Ultimately, 9 articles published between 2020 2022 presenting comparative data total 228 T2DM DR, 220 T2DM, 118 HCs were analyzed. All studies found distinctive microbial beta diversity vs. HC, characterized by an altered Firmicutes/Bacteroidetes ratio, decrease butyrate producers, increase LPS-expressing pro-inflammatory species Bacteroidetes Proteobacteria phyla. probiotic Bifidobacterium Lactobacillus decreased when T2DM. Gut influence health multiple ways represent future therapeutic target DR.

Язык: Английский

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