Lactobacillus rhamnosus GG cell-free supernatant as a novel anti-cancer adjuvant
Journal of Translational Medicine,
Год журнала:
2023,
Номер
21(1)
Опубликована: Март 14, 2023
Abstract
Background
Gut
microbiota
modulation
has
been
demonstrated
to
be
effective
in
protecting
patients
against
detrimental
effects
of
anti-cancer
therapies,
as
well
improve
the
efficacy
certain
treatments.
Among
most
characterized
probiotics,
Lactobacillus
rhamnosus
GG
(LGG)
is
currently
utilized
clinics
alleviate
diarrhea,
mucositis
or
intestinal
damage
which
might
associated
with
several
triggers,
including
Clostridium
difficile
infections,
inflammatory
gut
diseases,
antibiotic
consumption,
chemotherapy
radiation
therapy.
Here,
we
investigate
whether
LGG
cell-free
supernatant
(LGG-SN)
exert
anti-proliferative
activity
toward
colon
cancer
and
metastatic
melanoma
cells.
Moreover,
assess
potential
adjuvant
effect
LGG-SN
combination
drugs.
Methods
alone
either
5-Fuorouracil
Irinotecan
was
used
treat
human
cell
lines.
Dimethylimidazol-diphenyl
tetrazolium
bromide
assay
employed
detect
cellular
viability.
Trypan
blue
staining,
anti-cleaved
caspase-3
anti-total
versus
PARP
western
blots,
annexin
V/propidium
iodide
flow
cytometry
analyses
were
death.
Flow
measurement
DNA
content
(with
propidium
staining)
together
qPCR
analysis
cyclins
expression
cycle.
Results
We
demonstrate
that
able
selectively
reduce
viability
cells
a
concentration-dependent
way.
While
does
not
any
on
control
fibroblasts.
In
cells,
reduction
apoptosis
induction,
but
mitotic
arrest
G2/M
phase
Additionally,
sensitizes
both
5-Fluorouracil
Irinotecan,
thereby
showing
positive
synergistic
action.
Conclusion
Overall,
our
results
suggest
may
contain
one
more
bioactive
molecules
sensitize
chemotherapeutic
Thus,
could
proposed
an
ideal
candidate
for
ground-breaking
integrated
approaches
oncology,
chemotherapy-related
side
overcome
resistance
relapse
issues,
thus
ameliorating
therapeutic
response
patients.
Язык: Английский
Global research trends and focus on the link between rheumatoid arthritis and neutrophil extracellular traps: a bibliometric analysis from 1985 to 2023
Yonglong Chang,
Qinling Ou,
Chao Zhou
и другие.
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Авг. 23, 2023
Rheumatoid
arthritis
(RA)
is
an
autoimmune
disease
that
currently
has
unknown
cause
and
pathogenesis,
associated
with
many
complications
a
high
disability
rate.
The
neutrophil
extracellular
trap
network
(NETs)
newly
discovered
mechanism
allows
neutrophils
to
capture
kill
pathogens.
Multiple
studies
in
recent
years
have
highlighted
its
relevance
the
progression
of
rheumatoid
arthritis.
Despite
growing
number
indicating
crucial
role
NETs
RA,
there
been
no
bibliometric
review
research
hotspots
trends
this
area.
In
study,
we
retrieved
articles
related
RA
from
Web
Science
Core
Collection
(WoSCC)
database
1985
2023
used
visualization
tools
such
as
Citespace,
VOSviewer,
Tableau
Public,
Microsoft
Office
Excel
2021
analyze
data.
After
screening,
included
total
416
publications
involving
2,334
researchers
1,357
institutions
167
countries/regions,
relevant
published
219
journals.
U.S.,
China,
Germany
are
top
3
countries/regions
124,
57,
37
respectively.
Mariana
J.
Kaplan
most
author,
journals
Frontiers
Immunology
International
Journal
Molecular
Sciences
had
significant
impact
on
field.
clinical
application
PAD
enzymes
their
inhibitors,
drug
development
therapeutic
targets
for
trend
future
research.
Our
study
provides
comprehensive
analysis
summary
publications,
which
will
aid
conducting
further
scientific
Язык: Английский
Mapping trends and hotspot regarding gastrointestinal microbiome and neuroscience: A bibliometric analysis of global research (2002–2022)
Frontiers in Neuroscience,
Год журнала:
2022,
Номер
16
Опубликована: Ноя. 17, 2022
Scholars
have
long
understood
that
gastrointestinal
microorganisms
are
intimately
related
to
human
disorders.
The
literature
on
research
involving
the
gut
microbiome
and
neuroscience
is
emerging.
This
study
exposed
connections
between
microbiota
methodically
intuitively
using
bibliometrics
visualization.
study's
objectives
were
summarize
knowledge
structure
identify
emerging
trends
potential
hotspots
in
this
field.On
October
18,
2022,
a
search
was
conducted
utilizing
Web
of
Science
Core
Collection
(WoSCC)
database
for
studies
from
2002
2022
(August
20,
2022).
VOSviewer
CiteSpace
V
software
used
conduct
visualization
analysis.From
2022),
2,275
publications
WoSCC
satisfied
criteria.
annual
volume
has
rapidly
emerged
recent
years
(2016-2022).
most
productive
nation
(n
=
732,
32.18%)
hub
inter-country
cooperation
(links:
38)
United
States.
University
College
Cork
had
papers
published
area,
followed
by
McMaster
Harvard
Medical
School.
Cryan
JF,
Dinan
TG,
Clarke
G
key
researchers
with
considerable
academic
influence.
journals
"Neurogastroenterology
Motility"
"Brain
Behavior
Immunity."
cited
article
co-cited
reference
JF's
2012
impact
brain
behavior.
current
hotspot
includes
microbiome,
inflammation,
gut-brain
axis,
Parkinson's
disease
(PD),
Alzheimer's
(AD).
focus
would
be
"gastrointestinal
inflammation:
link
obesity,
insulin
resistance,
cognition"
"the
role
two
important
theories
axis
microbial-gut-brain
diseases."
Burst
detection
analysis
showed
schizophrenia,
pathology,
psychiatric
disorder
may
continue
frontiers.Research
diseases"
will
hotspot.
Schizophrenia
diseases
field
neuroscience,
pathology
content,
which
worthy
scholars'
attention.
Язык: Английский
Global cluster analysis and network visualization in organoids in cancer research: a scientometric mapping from 1991 to 2021
Frontiers in Oncology,
Год журнала:
2023,
Номер
13
Опубликована: Сен. 15, 2023
In
the
last
three
decades,
there
has
been
a
surge
in
research
on
cancer
organoids
using
3D
culture
technologies,
which
resulted
development
of
physiological
human
models.
This
study
aims
to
provide
an
overview
global
trends
and
frontiers
organoids.A
total
3189
publications
from
1991
2021
were
collected
Science
Citation
Index-Expanded
(SCIE)
Web
(WoS).
Bibliometric
methods
such
as
R
package
"Bibliometrix,"
Citespace,
VOS
viewer
software
employed
investigate
visualize
bibliographic
coupling,
co-citation,
co-authorship,
co-occurrence
trends,
well
publication
field
research.From
2021,
significant
increase
organoids,
with
most
articles
being
North
America,
Eastern
Asia,
Western
Europe.
The
USA
had
highest
number
publications,
citations,
prolific
authors,
funding
globally.
Cancers
was
journal
while
Nature
best
link
strength.
Harvard
University
contributive
institutions.
this
could
be
classified
into
five
clusters:
chemotherapy
study,
for
drug
screening,
different
models,
molecular
mechanism
organoid
construction.
These
areas
are
expected
remain
hotspots
future
research.The
is
based
current
trends.
Язык: Английский
Mapping knowledge landscapes and research frontiers of gastrointestinal microbiota and bone metabolism: a text-mining study
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Июль 10, 2024
Extensive
research
efforts
have
been
dedicated
to
elucidating
the
intricate
pathways
by
which
gastrointestinal
microbiota
and
their
metabolites
exert
influence
on
processes
of
bone
formation.
Nonetheless,
a
notable
gap
exists
in
literature
concerning
bibliometric
analysis
trends
at
nexus
metabolism.
Язык: Английский
A bibliometric analysis of global research on short chain fatty acids in neurological diseases
Medicine,
Год журнала:
2024,
Номер
103(41), С. e40102 - e40102
Опубликована: Окт. 11, 2024
Background:
The
interest
on
short
chain
fatty
acids
(SCFAs)
regulating
the
progress
of
neurological
diseases
has
gained
significant
attention
in
recent
years.
This
bibliometric
analysis
aimed
to
provide
insights
into
current
state
and
future
trends
global
research
SCFAs
research.
Methods:
To
general
trend
publications,
scientific
output
this
field
from
1995
2024
was
first
retrieved
Web
Science
Core
Collection,
Scopus,
PubMed
with
SCFAs-related
related
terms
as
subjects.
Based
above
publication
analysis,
rapid
development
stage
marked
2016
878
relevant
original
articles
time
limit
2024.
Key
indicators
were
calculated
evaluated
using
CiteSpace
these
articles.
Results:
are
occurrence
diseases.
China
USA
have
contributed
a
way
foster
better
understanding
hot
theme
gradually
shifted
neurodegenerative
psychical
In
aspect
mechanism
research,
hotspot
is
inflammation.
able
modulate
oxidative
stress
microglia
maturation,
morphology
function
intervene
thus
interventions
promising
treat
fast-developing
field.
publications
dramatically
increased
high
quality.
Conclusion:
will
continue
be
an
active
area
near
future.
Future
might
correlation
neurotherapeutics
patients
deeper
still
needed.
Язык: Английский
Hotspots and Frontiers of Host Immune Response in Idiopathic Pulmonary Fibrosis: A Bibliometric and Scientific Visual Research from 2000 to 2022
Journal of Immunology Research,
Год журнала:
2023,
Номер
2023, С. 1 - 18
Опубликована: Апрель 19, 2023
Idiopathic
pulmonary
fibrosis
(IPF)
is
a
disease
with
significant
morbidity,
progressive
deterioration
of
lung
function
till
death,
and
lack
effective
treatment
options.
This
study
aims
to
explore
the
global
research
trends
in
IPF
immune
response
predict
hotspot
future.
Materials
methods.
All
related
publications
on
since
establishment
diagnostic
criteria
for
were
retrieved
using
Web
Science
(WOS)
database.
VOSviewer,
GraphPad
Prism
6,
CiteSpace
version
5.6.
R5
64-bit,
bibliometrics
online
platform
used
extract
analyze
relevant
fields.
From
March
1,
2000,
September
30,
2022,
total
658
articles
25,126
citations
met
inclusion
criteria.
The
United
States
ranked
first
number
(n
=
217),
14,745),
H-index
(62).
China
second
124)
seventh
fifth
citation
frequency
H-index,
respectively.
American
Journal
Respiratory
Critical
Care
Medicine
(impact
factor
30.528)
published
most
field.
author
Kaminski
N.
from
was
influential
26
an
24.
Among
52
keywords
that
co-occurred
at
least
20
times,
main
concentrated
"Inflammation
related"
"Biomarker
clusters.
"biomarker"
(AAY
2018.64,
25
times)
newly
emerged
keyword.
has
unequivocal
advantage
immunization,
but
shows
faster
developing
trend.
should
be
prioritized
leading
articles.
indicates
exploration
ideal
immune-related
biomarkers
provide
evidence
clinical
work
might
near
Язык: Английский
Tracing the evolving dynamics and research hotspots of microbiota and immune microenvironment from the past to the new era
Microbiology Spectrum,
Год журнала:
2023,
Номер
11(5)
Опубликована: Сен. 28, 2023
Gut
microbiota
can
regulate
many
physiological
processes
within
gastrointestinal
tract
and
other
distal
sites.
Dysbiosis
may
not
only
influence
chronic
diseases
like
the
inflammatory
bowel
disease
(IBD),
metabolic
disease,
tumor
its
therapeutic
efficacy,
but
also
deteriorate
acute
injuries.
This
article
aims
to
review
documents
in
this
field
summarize
research
hotspots
as
well
developing
processes.
immune
microenvironment-related
from
1976
2022
were
obtained
Web
of
Science
Core
Collection
database.
Bibliometrics
was
used
assess
core
authors
journals,
most
contributive
countries
affiliations
together
with
keyword
co-occurrence
analysis.
Data
visualized
help
comprehension.
Nine
hundred
twelve
about
gut
microenvironment
retrieved,
annual
publications
increased
gradually.
The
productive
author,
country,
affiliation
"Zitvogel
L,"
USA
"UNIV
TEXAS
MD
ANDERSON
CANC
CTR,"
respectively.
FRONTIERS
IN
IMMUNOLOGY,
CANCERS,
INTERNATIONAL
JOURNAL
OF
MOLECULAR
SCIENCE
periodicals
publications.
Keyword
analysis
identified
three
clusters,
including
microbiota,
inflammation,
IBD.
Combined
literature
reading,
we
recognized
key
topics
microbiota:
cancer
therapy;
immunity,
inflammation
IBD;
injuries
diseases.
revealed
researches
on
growing.
More
attention
should
be
given
latest
IBD,
immunotherapy,
traumas,
diseases.IMPORTANCEGut
While
application
bibliometrics
still
remains
blank,
which
focused
more
regulation
different
kinds
Here,
intended
presented
by
bibliometrics.
And
Язык: Английский
Global Trends in Research Regarding Macrophages Associated with Chronic Obstructive Pulmonary Disease: A Bibliometric Analysis from 2011 to 2022
International Journal of COPD,
Год журнала:
2023,
Номер
Volume 18, С. 2163 - 2177
Опубликована: Окт. 1, 2023
Purpose:
Chronic
obstructive
pulmonary
disease
(COPD)
is
a
prevalent
respiratory
condition
characterized
by
chronic
airway
inflammation,
where
macrophages
from
the
innate
immune
system
may
exert
pivotal
influence.
Our
study
aimed
to
summarize
present
state
of
knowledge
and
identify
focal
points
emerging
developments
regarding
associated
with
COPD
through
bibliometrics.
Methods:
Publications
research
on
January
1,
2011,
2022,
were
retrieved
Science
Citation
Index-Expanded
(SCI-E)
which
part
Web
database.
In
total,
1521
publications
analyzed
using
bibliometric
methodology.
VOSviewer
was
used
analyze
annual
publications,
countries,
institutions,
authors,
journals,
hotspots.
Results:
Based
analysis,
relating
tended
increase
2011
2022.
The
United
States
largest
producer
most
influential
country
in
this
field.
Research
during
past
decade
has
focused
inflammation
lungs.
Most
previous
studies
have
mainly
mechanisms
that
promote
initiation
progression
COPD.
Macrophage-related
oxidative
stress
immunity,
communication
between
epithelial
cells,
interventions
for
acute
exacerbations
become
focus
more
recent
will
hot
topic
future.
Conclusion:
Global
macrophage-associated
been
growing
rapidly
decade.
topics
field
gradually
shift
“inflammation”
“oxidative
stress”,
“epithelial-cells”,
“exacerbations”.
significance
coordinating
responses,
interacting
other
exhibiting
dysregulated
capacities
attracted
increasing
attention
pathogenesis.
adoption
new
technologies
provide
promising
comprehensive
understanding
specific
role
Keywords:
macrophages,
disease,
VOSviewer,
hotspots,
bibliometrix
Язык: Английский