International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(19), С. 14954 - 14954
Опубликована: Окт. 6, 2023
Alzheimer's
disease
(AD)
is
one
of
the
most
prevalent
neurodegenerative
diseases
and
a
major
contributor
to
dementia.
Although
cause
this
condition
has
been
identified
long
ago
as
aberrant
aggregations
amyloid
tau
proteins,
effective
therapies
for
it
remain
elusive.
The
complexities
drug
development
AD
treatment
are
often
compounded
by
impermeable
blood-brain
barrier
low-yield
brain
delivery.
In
addition,
use
high
concentrations
overcome
challenge
may
entail
side
effects.
To
address
these
challenges
enhance
precision
delivery
into
regions
affected
aggregation,
we
proposed
transferrin-conjugated
nanoparticle-based
system.
melittin-loaded
L-arginine-coated
iron
oxide
nanoparticles
(Tf-MeLioNs)
developed
in
study
successfully
mitigated
melittin-induced
cytotoxicity
hemolysis
cell
culture
5XFAD
mouse
brain,
Tf-MeLioNs
remarkably
reduced
plaque
accumulation,
particularly
hippocampus.
This
suggested
Tf-LioNs
potential
platform
candidate
therapeutic
targeting
plaques
AD.
These
findings
provide
foundation
further
exploration
advancement
therapeutics.
Cells,
Год журнала:
2022,
Номер
11(24), С. 3956 - 3956
Опубликована: Дек. 7, 2022
Microglia
play
a
vital
role
in
neurodegenerative
diseases.
However,
the
effects
of
microglia-derived
exosomes
on
neuronal
cells
are
poorly
understood.
This
study
aimed
to
explore
M1-polarized
microglia
by
transcriptome
analysis.
Exosomes
isolated
from
resting
M0-phenotype
BV2
(M0-BV2)
and
(M1-BV2)
were
analyzed
using
high-throughput
sequencing
transcriptome.
Differentially
expressed
genes
(DEGs)
between
two
types
identified
analyzing
data.
The
biological
functions
pathways
regulated
DEGs
then
bioinformatics
analyses.
Finally,
we
evaluated
coculturing
M0-BV2
M1-BV2
with
primary
cells.
Enrichment
analyses
revealed
that
significantly
enriched
ferroptosis
pathway
(p
=
0.0137).
had
no
distinct
cells,
whereas
reduced
suppressor
proteins
(GPX4,
SLC7A11,
FTH1)
elevated
levels
intracellular
mitochondrial
ferrous
iron
lipid
peroxidation
Polarized
can
induce
thereby
aggravating
damage.
Taken
together,
these
findings
enhance
knowledge
pathogenesis
neurological
disorders
suggest
potential
therapeutic
targets
against
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(6), С. 5259 - 5259
Опубликована: Март 9, 2023
Alzheimer's
disease
(AD)
is
a
chronic
neurodegenerative
characterized
by
the
occurrence
of
cognitive
deficits.
With
no
effective
treatments
available,
search
for
new
therapies
has
become
major
focus
interest.
In
present
study,
we
describe
potential
therapeutic
effect
Artemisia
annua
(A.
annua)
extract
on
AD.
Nine-month-old
female
3xTg
AD
mice
were
treated
with
A.
three
months
via
oral
administration.
Animals
assigned
to
WT
and
model
groups
administrated
an
equal
volume
water
same
period.
Treated
significantly
improved
deficits
exhibited
reduced
Aβ
accumulation,
hyper-phosphorylation
tau,
inflammatory
factor
release
apoptosis
when
compared
untreated
mice.
Moreover,
promoted
survival
proliferation
neural
progenitor
cells
(NPS)
increased
expression
synaptic
proteins.
Further
assessment
implicated
mechanisms
revealed
that
regulates
YAP
signaling
pathway
in
studies
comprised
incubation
PC12
Aβ1-42
at
concentration
8
μM
or
without
different
concentrations
24
h.
Obtained
ROS
levels,
mitochondrial
membrane
potential,
caspase-3
activity,
neuronal
cell
pathways
involved
was
performed
using
western
blot
immunofluorescence
staining.
The
obtained
results
showed
reversed
Aβ1-42-induced
increase
activity
vitro.
either
inhibition
pathway,
specific
inhibitor
CRISPR
cas9
knockout
gene,
neuroprotective
extract.
These
findings
suggest
may
be
multi-target
anti-AD
drug
use
prevention
treatment
Experimental Gerontology,
Год журнала:
2023,
Номер
175, С. 112139 - 112139
Опубликована: Март 10, 2023
Aging
is
an
important
risk
factor
for
neurodegenerative
diseases.
The
activation
of
α7
nicotinic
acetylcholine
receptor
(α7nAChR)
involved
in
inflammation
and
cognition,
but
the
specific
role
it
plays
aging
remains
unknown.
This
study
aimed
to
investigate
anti-aging
effect
α7nAChR
on
rats
BV2
cells
induced
by
D-galactose,
as
well
its
potential
mechanism.
D-galactose
increase
SA-β-Gal
positive
cells,
expression
p16
p21
vivo
vitro.
selective
agonist
PNU282987
decreased
levels
pro-inflammatory
factors,
MDA,
Aβ,
enhanced
SOD
activity
anti-inflammatory
(IL10)
vivo.
Arg1,
iNOS,
IL1β
TNFα
upregulated
α7nAChR,
Nrf2
HO-1
results
Morris
water
maze
novel
object
recognition
tests
showed
that
improved
cognitive
impairment
rats.
Furthermore,
inhibitor
methyllycaconitine
(MLA)
were
opposite
with
PNU282987.
improves
through
inhibiting
oxidative
stress
neuroinflammation
via
regulating
α7nAChR/Nrf2/HO-1
signaling
pathway.
Therefore,
targeting
may
be
a
viable
therapeutic
approach
anti-inflammaging
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(19), С. 14954 - 14954
Опубликована: Окт. 6, 2023
Alzheimer's
disease
(AD)
is
one
of
the
most
prevalent
neurodegenerative
diseases
and
a
major
contributor
to
dementia.
Although
cause
this
condition
has
been
identified
long
ago
as
aberrant
aggregations
amyloid
tau
proteins,
effective
therapies
for
it
remain
elusive.
The
complexities
drug
development
AD
treatment
are
often
compounded
by
impermeable
blood-brain
barrier
low-yield
brain
delivery.
In
addition,
use
high
concentrations
overcome
challenge
may
entail
side
effects.
To
address
these
challenges
enhance
precision
delivery
into
regions
affected
aggregation,
we
proposed
transferrin-conjugated
nanoparticle-based
system.
melittin-loaded
L-arginine-coated
iron
oxide
nanoparticles
(Tf-MeLioNs)
developed
in
study
successfully
mitigated
melittin-induced
cytotoxicity
hemolysis
cell
culture
5XFAD
mouse
brain,
Tf-MeLioNs
remarkably
reduced
plaque
accumulation,
particularly
hippocampus.
This
suggested
Tf-LioNs
potential
platform
candidate
therapeutic
targeting
plaques
AD.
These
findings
provide
foundation
further
exploration
advancement
therapeutics.
