Human Gene Therapy, Год журнала: 2023, Номер 34(9-10), С. 416 - 429
Опубликована: Апрель 8, 2023
Optogenetics is a technique where cell transduced with light-sensitive ion channel. This can be used to control muscle contraction in conjunction commonly viral vectors. However, this has not yet become widely applied. In study, we discuss the mechanisms and techniques involved opsin transfer tissue, clinical applicability of these approaches, major limitations facing technique.
Язык: Английский
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 6, 2025
High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations, and especially powerful when combined with optogenetics manipulation of specific cell types high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic photonic circuits. These devices pack 960 electrical sites two sets 14 light emitters onto a 1 cm shank, allowing spatially addressable optogenetic stimulation blue red light. In mouse cortex, probes delivered high-quality recordings together optogenetics, differentially activating or silencing neurons at distinct cortical depths. striatum other deep structures, efficient optotagging, facilitating identification in parallel. represent an unprecedented tool recording, identifying, manipulating neuronal populations.
Язык: Английский
Процитировано
5
Coordination Chemistry Reviews, Год журнала: 2023, Номер 493, С. 215340 - 215340
Опубликована: Июль 13, 2023
Язык: Английский
Процитировано
40
Nature Communications, Год журнала: 2022, Номер 13(1)
Опубликована: Ноя. 4, 2022
Abstract The understanding of how the rhodopsin sequence can be modified to exactly modulate spectroscopic properties its retinal chromophore, is a prerequisite for rational design more effective optogenetic tools. One key problem that establishing rules satisfied achieving highly fluorescent rhodopsins with near infrared absorption. In present paper we use multi-configurational quantum chemistry construct computer model recently discovered natural rhodopsin, Neorhodopsin, displaying such properties. We show model, successfully replicates relevant experimental observables, unveils geometrical and electronic structure chromophore featuring diffuse charge distribution along conjugated chain. same reveals confinement process occurring excited state isomerization coordinate, primary cause observed fluorescence enhancement.
Язык: Английский
Процитировано
33
ACS Nano, Год журнала: 2024, Номер 18(22), С. 14123 - 14144
Опубликована: Май 20, 2024
Optogenetic, known as the method of 21 centuries, combines optic and genetic engineering to precisely control photosensitive proteins for manipulation a broad range cellular functions, such flux ions, protein oligomerization dissociation, intercommunication, so on. In this technique, light is conventionally delivered targeted cells through optical fibers or micro light-emitting diodes, always suffering from high invasiveness, wide-field illumination facula, strong absorption, scattering by nontargeted endogenous substance. Light-transducing nanomaterials with advantages spatiotemporal resolution, abundant wireless-excitation manners, easy functionalization recognition specific cells, recently have been widely explored in field optogenetics; however, there remain few challenges restrain its clinical applications. This review summarized recent progress on light-responsive genetically encoded myriad activation strategies use light-transducing their disease-treatment applications, which expected sparking helpful thought push forward preclinical translational uses.
Язык: Английский
Процитировано
8
Frontiers in Chemistry, Год журнала: 2022, Номер 10
Опубликована: Июнь 22, 2022
The first member and eponym of the rhodopsin family was identified in 1930s as visual pigment rod photoreceptor cell animal retina. It found to be a membrane protein, owing its photosensitivity presence covalently bound chromophoric group. This group, derived from vitamin A, appropriately dubbed retinal. In 1970s microbial counterpart this species discovered an archaeon, being protein also harbouring retinal chromophore, named bacteriorhodopsin. Since their discovery photogenic panorama unfolded, where up date new members subspecies with variety light-driven functionality have been added family. branch, meanwhile categorized type-2 rhodopsins, turned out form large subclass superfamily G protein-coupled receptors are essential multiple elements light-dependent sensory physiology. type-1 largely function ion pumps or channels, but contain sensory-active enzyme-sustaining subspecies. review we will follow development exciting representative number highlights present prospect extraordinary future potential.
Язык: Английский
Процитировано
28
Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10
Опубликована: Окт. 14, 2022
Numerous photoreceptors and genetic circuits emerged over the past two decades now enable light-dependent i.e., optogenetic, regulation of gene expression in bacteria. Prompted by light cues near-ultraviolet to near-infrared region electromagnetic spectrum, can be up- or downregulated stringently, reversibly, non-invasively, with precision space time. Here, we survey underlying principles, available options, prominent examples optogenetically regulated While transcription initiation elongation remain most important for optogenetic intervention, other processes e.g., translation downstream events, were also rendered light-dependent. The control bacterial predominantly employs but three fundamental strategies: light-sensitive two-component systems, oligomerization reactions, second-messenger signaling. Certain moved beyond proof-of-principle stood test practice. They unprecedented applications major areas. First, underpins novel concepts strategies enhanced yields microbial production processes. Second, light-responsive bacteria stimulated while residing within bodies animals, thus prompting secretion compounds that grant health benefits animal host. Third, optogenetics allows generation precisely structured, biomaterials. These jointly testify maturity approach serve as blueprints bound inspire template innovative use cases light-regulated Researchers pursuing these lines choose from an ever-growing, versatile, efficient toolkit circuits.
Язык: Английский
Процитировано
27
ACS Synthetic Biology, Год журнала: 2022, Номер 11(10), С. 3354 - 3367
Опубликована: Авг. 23, 2022
In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) sense red and far-red light via a biliverdin chromophore and, response, cycle between the spectroscopically, structurally, functionally distinct Pr Pfr states. BphPs commonly belong two-component systems that phosphorylation of cognate response regulators downstream gene expression through histidine kinase modules. We recently demonstrated paradigm BphP from
Язык: Английский
Процитировано
25
Molecular Psychiatry, Год журнала: 2023, Номер 28(10), С. 4407 - 4420
Опубликована: Март 23, 2023
The study of Alzheimer's Disease (AD) has traditionally focused on neuropathological mechanisms that guided therapies attenuate features. A new direction is emerging in AD research focuses the progressive loss cognitive function due to disrupted neural circuit mechanisms. Evidence from humans and animal models show dysregulated circuits initiate a cascade pathological events culminate functional learning, memory, other aspects cognition. Recent progress single-cell, spatial, omics informs this circuit-focused approach by determining identities, locations, circuitry specific cells affected AD. Recently developed neuroscience tools allow for precise access cell type-specific so their roles AD-related deficits disease progression can be tested. An integrated systems-level understanding AD-associated requires multimodal multi-scale interrogations longitudinally measure and/or manipulate ensemble properties molecularly-defined neuron populations first susceptible These newly technological conceptual advances present opportunities studying treating vulnerable represent beginning era circuit-based research.
Язык: Английский
Процитировано
17
Neurobiology of Disease, Год журнала: 2023, Номер 184, С. 106234 - 106234
Опубликована: Июль 20, 2023
The advent of optogenetic tools has had a profound impact on modern neuroscience research, revolutionizing our understanding the brain. These offer remarkable ability to precisely manipulate specific groups neurons with an unprecedented level temporal precision, order milliseconds. This breakthrough significantly advanced knowledge various physiological and pathophysiological processes in Within realm epilepsy have played crucial role investigating contributions different neuronal populations generation seizures hyperexcitability. By selectively activating or inhibiting using optogenetics, researchers been able elucidate underlying mechanisms identify key players involved epileptic activity. Moreover, techniques also explored as innovative therapeutic strategies for treating epilepsy. aim halt seizure progression alleviate symptoms by utilizing precise control offered optogenetics. application provided valuable insights into intricate workings brain during episodes. For instance, discovered how distinct interneuron contribute initiation (ictogenesis). They revealed remote circuits regions such cerebellum, septum, raphe nuclei can interact hyperexcitable networks hippocampus. Additionally, studies demonstrated potential closed-loop systems, where optogenetics is combined real-time monitoring, enable precise, on-demand Despite immense promise approaches, it important acknowledge that many these are still early stages development yet reach clinical applications. transition from experimental research practical use poses numerous challenges. In this review, we introduce tools, provide comprehensive survey their critically discuss current limitations achieving successful implementation treatment human addressing aspects, hope foster deeper state future prospects treatment.
Язык: Английский
Процитировано
15
Nature Reviews Bioengineering, Год журнала: 2024, Номер 2(10), С. 829 - 848
Опубликована: Июль 18, 2024
Язык: Английский
Процитировано
6