Biotechnology and Bioprocess Engineering, Год журнала: 2023, Номер 28(5), С. 761 - 773
Опубликована: Окт. 1, 2023
Язык: Английский
Human Immunology, Год журнала: 2025, Номер 86(3), С. 111269 - 111269
Опубликована: Фев. 24, 2025
Язык: Английский
Процитировано
1
Pharmacological Research, Год журнала: 2022, Номер 186, С. 106535 - 106535
Опубликована: Ноя. 2, 2022
Язык: Английский
Процитировано
29
Molecular Therapy, Год журнала: 2023, Номер 31(6), С. 1562 - 1576
Опубликована: Апрель 27, 2023
Non-coding RNAs, including long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and circular (circRNAs), are being studied extensively in a variety of fields. Their roles metabolism have received increasing attention recent years but not yet clear. The regulation glucose, fatty acid, amino acid is an imperative physiological process that occurs living organisms takes part cancer cardiovascular diseases. Here, we summarize the important played by glucose metabolism, as well mechanisms involved. We also therapeutic advances for diseases such obesity, disease, some metabolic Overall, indispensable factors significant role three major metabolisms, which may be exploited targets future.
Язык: Английский
Процитировано
22
Cell Death and Disease, Год журнала: 2023, Номер 14(4)
Опубликована: Апрель 6, 2023
Abstract Melatonin is a well-known natural hormone, which shows potential anticancer effect in many human cancers. Bladder cancer (BLCA) one of the most malignant cancers world. Chemoresistance an increasingly prominent phenomenon that presents obstacle to clinical treatment BLCA. There urgent need investigate novel drugs improve current status. In our study, we comprehensively explored inhibitory melatonin on BLCA and found it could suppress glycolysis process. Moreover, discovered ENO1, glycolytic enzyme involved ninth step glycolysis, was downstream effector be predictive biomarker We also proved enhanced simulated by adding exogenous pyruvate induce gemcitabine resistance, or silencing ENO1 intensify cytotoxic cells. Excessive accumulation reactive oxygen species (ROS) mediated Additionally, uncovered PPARγ upstream regulator downregulation caused melatonin. Our study offers fresh perspective encourages further studies chemoresistance.
Язык: Английский
Процитировано
15
Tissue and Cell, Год журнала: 2025, Номер 93, С. 102729 - 102729
Опубликована: Янв. 10, 2025
Colorectal cancer (CRC) is one of the aggressive malignant tumors. Studies have shown that glycolysis promotes proliferation colorectal cells and PYCR2 involved in progression by affecting cellular glycolysis. In addition, upregulated cell lines can affect autophagy. Si-PYCR2 was used to interfere with cells, were treated addition autophagy inhibitor 3-MA or mTOR agonist MHY1485. The expression LC3B detected immunofluorescence, glycolytic proteins Western blot. XF96 extracellular flux analyzer detect ECAR OCR biochemical kits levels glucose consumption, lactate secretion, ATP production cells. up-regulated lines. si-PYCR2 interference enhanced fluorescence intensity inhibited p62 but ATG5, ATG7, LC3-II/I proteins, which indicated an level depletion also as well mTOR/HK2 signaling. However, resulted increase while a decrease OCR, production, expressions (GLUT1, PGK1, ENO1, PKM2), suggested enhanced. MHY1485 treatment not only Interference corrected autophagy-dependent via Activation signaling mitigated effects
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Март 28, 2025
Abstract The mammalian brain relies primarily on glucose for its energy needs. Delivery of this nutrient to the is mediated by transporter-1 (Glut1) protein. Low Glut1 thwarts entry into brain, causing an crisis and, triggering, in one instance, debilitating neurodevelopmental condition – deficiency syndrome (Glut1DS). Current treatments Glut1DS are sub-optimal, as none address root cause low condition. Levels transporter must respond rapidly brain’s changing requirements. This necessitates fine-tuning expression. Here we describe a long-noncoding RNA (lncRNA) antisense and show that it involved such regulation. Raising levels lncRNA had concordant effect cultured human cells transgenic mice; reducing elicited opposite effect. Delivering model mice via viral vectors induced expression, enhancing mitigate disease. Direct delivery combat disease has not been reported previously constitutes unique therapeutic paradigm. Moreover, considering importance maintaining homeostatic levels, calibrating expression could become broadly relevant myriad conditions, including Alzheimer’s disease, wherein concentrations perturbed.
Язык: Английский
Процитировано
0
Molecular Medicine, Год журнала: 2025, Номер 31(1)
Опубликована: Апрель 22, 2025
Abstract In the clinical management of cancers, emergence chemoresistance represents a profound and imperative “pain point” that requires immediate attention. Understanding mechanisms is essential for developing effective therapeutic strategies. Importantly, existing studies have demonstrated glucose metabolic reprogramming, commonly referred to as Warburg effect or aerobic glycolysis, major contributor chemoresistance. Additionally, lactate, byproduct functions signaling molecule supports lysine lactylation modification proteins, which also plays critical role in However, it insufficient discuss glycolysis from single perspective. The intricate relationship between crucial promoting Thus, thorough elucidation underlying mediated by essential. This review provides comprehensive overview these further outlines exert synergistic effects, development creating positive feedback loop continues mediate this resistance. close link suggests application glycolysis-related drugs inhibitors cancer therapy may represent promising anticancer strategy. Furthermore, targeted lactylation, either alone combination with other treatments, offer new avenues overcoming
Язык: Английский
Процитировано
0
Non-coding RNA Research, Год журнала: 2024, Номер 10, С. 1 - 15
Опубликована: Авг. 9, 2024
Cancer progression results from the dysregulation of molecular pathways, each with unique features that can either promote or inhibit tumor growth. The complexity carcinogenesis makes it challenging for researchers to target all pathways in cancer therapy, emphasizing importance focusing on specific targeted treatment. One such pathway is PI3K/Akt pathway, which often overexpressed cancer. As cells progress, expression increases, further driving advancement. This study aims explore how ncRNAs regulate PI3K/Akt. NcRNAs are found both cytoplasm and nucleus, their functions vary depending location. They bind promoters PI3K Akt, reducing increasing expression, thus influencing tumorigenesis. ncRNA/PI3K/Akt axis plays a crucial role determining cell proliferation, metastasis, epithelial-mesenchymal transition (EMT), even chemoresistance radioresistance human cancers. Anti-tumor compounds modulate axis. Moreover, directly indirectly.
Язык: Английский
Процитировано
3
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Фев. 23, 2024
ABSTRACT Endometrial cancer is one of the most common gynecological cancers in world, with an estimated 382,000 new cases and 90,000 deaths each year. There no specific treatment, as underlying causes endometrial neoplasia are poorly understood. This study focuses on development validation a three-dimensional (3D) vitro multilayer, multicellularhydrogel that facilitates drug screening analysis. We hypothesized combination natural (collagen type I IV, fibrinogen, fibronectin, Laminin) synthetic (GELMA, PEGDA) polymers would maximize microvessel formation cell invasion. The 3D model incorporates human microvascular endothelial cells (hMVEC) (HEC-1A) atop hydrogel formulations mimicking cell-specific extracellular matrix components. Using D-optimal experimental design, 45 combinations were generated. predicted formulation to all responses enhanced higher invasion compared gold standard Matrigel. Subsequent emphasizes importance disease-specific crosstalk maximizing optimized adeptly captures variances among lines from distinct stages. Finally, platform employed compare viability, formation, across Ishikawa, KLE, HEC-1A after Paclitaxel exposure, delivered both free loaded poly(caprolactone) (PCL) nanoparticles. Overall, this provides valuable tool for exploring intricate interactions within tumor microenvironment, offering holistic understanding fostering targeted therapeutic strategies cancer.
Язык: Английский
Процитировано
2
Radiology Imaging Cancer, Год журнала: 2024, Номер 6(2)
Опубликована: Март 1, 2024
Purpose To characterize the metabolomic profiles of two hepatocellular carcinoma (HCC) rat models, track evolution these to a stimulated tumor state, and assess their effect on lactate flux with hyperpolarized (HP) carbon 13 (13C) MRI. Materials Methods Forty-three female adult Fischer rats were implanted N1S1 or McA-RH7777 HCC tumors. In vivo lactate-to-pyruvate ratio (LPR) was measured HP 13C MRI at 9.4 T. Ex mass spectrometry used measure intratumoral metabolites, Ki67 labeling quantify proliferation. Tumors first compared three normal liver controls. The tumors then variants via off-target hepatic thermal ablation treatment. All comparisons made using Mann–Whitney test. Results pyruvate showed greater LPR in (mean [SD], 0.564 ± 0.194 vs 0.311 0.057; P < .001 [n = 9]), but not for (P .44 8]). Mass confirmed that glycolysis pathway increased decreased pentose phosphate also only Increased proliferation corresponded net increase (six six nonstimulated, 0.269 0.148 0.027 0.08; .009), (eight .13), despite metastases. demonstrated relatively production stimulation only. Conclusion Two subtypes divergent glycolytic dependency baseline during transformation high state. This metabolic heterogeneity should be considered use diagnosis tracking. Keywords: Molecular Imaging-Probe Development, Liver, Abdomen/GI, Oncology, Hepatocellular Carcinoma © RSNA, 2024 See commentary by Ohliger this issue.
Язык: Английский
Процитировано
2