Integration of network pharmacology and experimental verifications reveals the Bian-Se-Tong mixture can alleviate constipation in STC rats by reducing apoptosis of Cajal cells via activating PI3K-Akt signaling pathway

Heliyon, Год журнала: 2024, Номер 10(7), С. e28022 - e28022

Опубликована: Март 13, 2024

Bian-Se-Tong mixture (BSTM) is an optimized formulation based on the classical prescription "Zhizhu pill", which widely used in clinical treatment of slow-transit constipation (STC). The potential molecular mechanism BSTM therapy for STC was investigated by network pharmacology prediction combined with animal experiments. active components were screened via TCMSP platform. GeneCards, OMIM and DrugBank databases to search targets. With help Biogenet tool, a protein interaction between drugs disease targets constructed, intersection two extracted obtain key STC. GO KEGG enrichment analyses carried out Metascape. Loperamide hydrochloride establish rat model, related pathways preliminarily verified. important signaling included PI3K-Akt, MAPK, IL-17, cAMP, cell cycle pathways. experimental results showed that increased body weight rats fecal particle number, water content intestinal carbon ink promotion rate within 24 h. Further pathological changes colon also observed. In-depth mechanistic studies have shown can significantly reduce apoptosis Cajal cells, downregulate expression Bax c-Caspase 3, upregulate Bcl-2 c-kit, promote phosphorylation AKT. relieve activating PI3K-Akt pathway improving apoptosis.

Язык: Английский

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Pungent agents derived from the fruits of Zanthoxylum armatum DC. are beneficial for ameliorating type 2 diabetes mellitus via regulation of AMPK/PI3K/Akt signaling

Journal of Functional Foods, Год журнала: 2024, Номер 116, С. 106160 - 106160

Опубликована: Апрель 9, 2024

Type 2 diabetes mellitus (T2DM), the main type of diabetes, is a common chronic metabolic disease and serious health concern worldwide. The aim this study was to investigate anti-diabetic effects underlying mechanisms pungent substances in Zanthoxylum armatum DC (ZA) fruit (PAZ), which are both edible medicinal. We firstly investigated possible molecular PAZ on T2DM using network pharmacology online databases. Furthermore, predicted results were explored by Glucosamine induced AML12 experiments vitro. After intervention, ROS levels decreased, MDA oxidative stress indicators SOD, GSH, CAT increased compared model group. Thus, agents from fruits Z. beneficial for ameliorating via regulation AMPK/PI3K/AKT signaling reducing exerting an anti-insulin resistance effect.

Язык: Английский

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Exploration of Polysaccharides from Phyllanthus emblica: Isolation, Identification, and Evaluation of Antioxidant and Anti-Glycolipid Metabolism Disorder Activities

Molecules, Год журнала: 2024, Номер 29(8), С. 1751 - 1751

Опубликована: Апрель 12, 2024

Phyllanthus emblica is a natural medicinal herb with diverse bioactivities. Certain extracts from this have been confirmed to possess anti-glycolipid metabolic disorder activity. To further develop its utility value and explore potential in combating glycolipid disorders, we designed series of experiments investigate the structure, antioxidant activity, activity polysaccharides. In study, extracted purified polysaccharides thoroughly analyzed their structure using various techniques, including NMR, methylation analysis, surface-enhanced Raman spectroscopy. We investigated hypolipidemic metabolism for first time utilizing oleic acid (OA) advanced glycation end products (AGEs) as inducers. Additionally, was assessed vitro. These findings lay groundwork future investigations into application an intervention preventing treating diabetes.

Язык: Английский

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Identification of Antioxidant Methyl Derivatives of Ortho-Carbonyl Hydroquinones That Reduce Caco-2 Cell Energetic Metabolism and Alpha-Glucosidase Activity

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(15), С. 8334 - 8334

Опубликована: Июль 30, 2024

α-glucosidase, a pharmacological target for type 2 diabetes mellitus (T2DM), is present in the intestinal brush border membrane and catalyzes hydrolysis of sugar linkages during carbohydrate digestion. Since α-glucosidase inhibitors (AGIs) modulate metabolism, they may influence oxidative stress glycolysis inhibition, potentially addressing dysfunction associated with T2DM. Herein, we report on study an

Язык: Английский

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Comprehensive metabolome characterization and comparison between two sources of Dragon’s blood by integrating liquid chromatography/mass spectrometry and chemometrics

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер 416(7), С. 1571 - 1587

Опубликована: Янв. 27, 2024

Язык: Английский

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Integrating network pharmacology and experimental studies for uncovering the molecular mechanisms of Dioscorea bulbifera L. in the treatment of thyroid cancer

Heliyon, Год журнала: 2023, Номер 9(8), С. e18886 - e18886

Опубликована: Авг. 1, 2023

IntroductionThyroid cancer (TC), a common endocrine malignant tumor with higher incidence in females than males, is serious threat to human life and health. Although current clinical treatments can alleviate this disease, the recurrence rate remains high. Tuber Dioscoreae Bulbiferae, also called Huang-Yao-Zi (HYZ), has remarkable curative properties, few side effects used for treatment of sore throat, goiter, hemoptysis, other diseases traditional Chinese medicine (TCM). Existing studies have found that HYZ improve symptoms TC patients reduce volume, while vitro induce death thyroid cells. However, mechanism still unclear.MethodsIn study, based on network pharmacology bioinformatics, target molecular were preliminarily explored. The results suggest antithyroid effect may be related PI3K-Akt focal adhesion pathways. Then, cell model was established further explore detail mechanisms.ResultsFortunately, induced apoptosis KMH-2 cells regulated expression apoptosis-related proteins genes. At same time, significantly inhibit migration invasion Further showed pharmacological activities regulation pathways.ConclusionOur study provides reference animal or investigating effectiveness mechanisms against cancer.

Язык: Английский

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Licochalcone A induces G2/M phase arrest and apoptosis via regulating p53 pathways in esophageal cancer: In-vitro and in-vivo study

European Journal of Pharmacology, Год журнала: 2023, Номер 958, С. 176080 - 176080

Опубликована: Сен. 25, 2023

Язык: Английский

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In Vitro Metabolism and In Vivo Pharmacokinetics Profiles of Hydroxy-α-Sanshool

Toxics, Год журнала: 2024, Номер 12(2), С. 100 - 100

Опубликована: Янв. 24, 2024

Hydroxy-α-sanshool (HAS) is the predominant active compound in Zanthoxylum bungeanum Maxim (ZBM). Our present work was aimed to explore vitro metabolism characteristics, and vivo pharmacokinetic (PK) profile of HAS. Plasma (human), liver microsomes, hepatocytes (human, monkey, dog, mouse, rat) were collected for HAS studies elimination rates microsomes different species investigated. In addition, five recombinant human CYP enzymes used identify isoforms Finally, PK properties rats studied by oral administration (p.o.). The results showed that stably metabolized rat hepatocytes, binding plasma proteins nonspecific; has strong inhibitory effects on CYP2C9 CYP2D6 microsomes. study, rapidly absorbed after administration. conclusion, metabolic this study provide data support its further development application, profiles can be as a reference safety evaluation.

Язык: Английский

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Zuojin Pill inhibits the proliferation of esophageal cancer cells by inducing ROS production and apoptosis via regulation of PI3K/Akt signalling

Journal of Taibah University for Science, Год журнала: 2024, Номер 18(1)

Опубликована: Июнь 14, 2024

This study aimed to investigate the inhibitory effect of Zuojin Pill (ZJP) on KYSE 150 cells and xenograft tumours digestive tract cancer – esophageal (EC) its underlying mechanism. Initially, in vivo research demonstrated that oral administration ZJP effectively inhibited growth EC tumours, vitro studies revealed suppressed proliferation, migration, invasion cells. Then, network pharmacology prediction identified core target, active ingredient, involvement PI3K-Akt signalling pathway treatment EC. Cell validated affected expression targets, ingredients proliferation Additionally, was shown induce cell apoptosis, decrease mitochondrial membrane potential, increase level intracellular oxidative stress. Further experimental verifications suggested effects might be attributed modulation PI3 K/Akt pathways.

Язык: Английский

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Targeting AMPK related signaling pathways: A feasible approach for natural herbal medicines to intervene non-alcoholic fatty liver disease

Journal of Pharmaceutical Analysis, Год журнала: 2024, Номер 15(1), С. 101052 - 101052

Опубликована: Авг. 6, 2024

Non-alcoholic fatty liver disease (NAFLD) is a metabolic characterized by abnormal deposition of lipid in hepatocytes. If not intervened time, NAFLD may develop into fibrosis or cancer, and ultimately threatening life. has complicated etiology pathogenesis, there are no effective therapeutic means specific drugs. Currently, insulin sensitizers, lipid-lowering agents hepatoprotective often used for clinical intervention, but these drugs have obvious side effects, their effectiveness safety need to be further confirmed. AMP-activated protein kinase (AMPK) plays central role maintaining energy homeostasis. Activated AMPK can enhanace degradation, alleviate resistance, suppress oxidative stress inflammatory response, regulate autophagy, thereby alleviating NAFLD. Natural herbal medicines received extensive attention recently because regulatory effects on low effects. In this article, we reviewed the biologically active natural (such as medicine formulas, extracts, polysaccharides, monomers) that reported recent years treat via regulating AMPK, which serve foundation subsequent development candidate

Язык: Английский

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