International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 11 - 11
Опубликована: Дек. 24, 2024
A
phage–antibiotic
synergy
could
be
an
alternative
in
urinary
tract
infection
(UTI)
therapy,
as
it
leads
to
the
elimination
of
bacteria
and
reduction
variants
resistant
phages
antibiotics.
The
aims
vitro
study
were
determine
whether
vB_Efa29212_2e
vB_Efa29212_3e
interact
synergistically
with
selected
antibiotics
treatment
E.
faecalis
infections,
optimize
antibiotic
concentrations
phage
titers
for
most
effective
combinations,
assess
their
impact
on
number
spontaneous
phages’
reproductive
cycles.
modified
double-layer
disc
diffusion
method,
checkboard,
time–kill
assays,
one-step
growth
method
double
agar
overlay
plaque
assay
implemented.
Synergistic
interactions
often
observed
after
combined
action
2e
or
3e
β-lactam
strains.
beneficial
effects
depended
bacterial
strain,
used.
lowest
minimum
inhibitory
concentration
(MIC50)
values
recorded,
application
low
2e,
high
3e.
use
tested
agents
resulted
a
significant
had
cycle
phages,
e.g.,
50%
increase
burst
size,
5
min
latency
period
observed.
confirmed
between
against
growth.
Pathogens,
Год журнала:
2024,
Номер
13(6), С. 522 - 522
Опубликована: Июнь 20, 2024
The
emergence
of
multidrug-resistant
bacteria
is
undoubtedly
one
the
most
serious
global
health
threats.
One
response
to
this
threat
that
has
been
gaining
momentum
over
past
decade
'phage
therapy'.
According
this,
lytic
bacteriophages
are
used
for
treatment
bacterial
infections,
either
alone
or
in
combination
with
antimicrobial
agents.
However,
ensure
efficacy
and
broad
applicability
phage
therapy,
several
challenges
must
be
overcome.
These
encompass
development
methods
strategies
host
range
manipulation
bypass
resistance
mechanisms
developed
by
pathogenic
bacteria,
as
case
since
advent
antibiotics.
As
our
knowledge
understanding
interactions
between
phages
their
hosts
evolves,
key
issue
define
each
application.
In
article,
we
discuss
factors
affect
how
determines
classification
into
different
categories
action.
For
group,
recent
representative
examples
provided,
together
suggestions
on
groups
can
combat
certain
types
infections.
available
methodologies
expansion,
through
sequential
adaptation
a
new
pathogen
genetic
engineering
techniques,
also
reviewed.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(15), С. 8321 - 8321
Опубликована: Июль 30, 2024
Patients
with
cystic
fibrosis
(CF)
are
prone
to
developing
life-threatening
lung
infections
a
variety
of
pathogens
that
difficult
eradicate,
such
as
Burkholderia
cepacia
complex
(Bcc),
Hemophilus
influenzae,
Mycobacterium
abscessus
(Mab),
Pseudomonas
aeruginosa,
and
Staphylococcus
aureus.
These
still
remain
an
important
issue,
despite
the
therapy
for
CF
having
considerably
improved
in
recent
years.
Moreover,
prolonged
exposure
antibiotics
combination
favors
development
spread
multi-resistant
bacteria;
thus,
alternative
strategies
is
crucial
counter
antimicrobial
resistance.
In
this
context,
phage
therapy,
i.e.,
use
phages,
viruses
specifically
infect
bacteria,
has
become
promising
strategy.
review,
we
aim
address
current
status
management
multidrug-resistant
infections,
from
compassionate
cases
ongoing
clinical
trials,
well
challenges
approach
presents
particular
context
patients.
Saudi Journal of Biological Sciences,
Год журнала:
2024,
Номер
31(6), С. 104002 - 104002
Опубликована: Апрель 25, 2024
American
foulbrood
(AFB)
is
a
harmful
honeybee
disease
primarily
caused
by
Paenibacillus
larvae.
The
study
aims
to
isolate
and
identify
the
AFB
causative
agent
P.
larvae
their
specific
phages
use
as
new
biological
method
for
control.
Eight
apiaries
were
inspected
infections.
Symptoms
of
diseased
brood
comb,
odd
cells
with
soft
brown
decayed
amongst
healthy
brood,
identified
in
field
demonstrated
prevalence
every
apiary.
Three
isolates
using
traditional
techniques
452-bp
PCR
amplicon
bacterial
16SrRNA
gene
was
compared
between
isolates.
Additionally,
strains
applied
examine
efficiency
reducing
infection
rate
under
apiary
condition.
reduced
approximately
94.6
100
%
through
application
phage
mixture,
opposed
20
85.7
when
each
administered
individually
or
78.6
88.9
antibiotic
treatment
implemented.
Histological
studies
on
phage-treated
bee
revealed
some
regaining
normal
shape,
prominent
nuclei
microvilli.
gastrointestinal
tract
showed
longitudinal
circular
muscles,
unlike
treated
abnormal
destroyed
tissues,
shown
basement
membrane
surrounding
mid-gut
epithelium.
Phage
exhibited
promise
resolving
issue
honeybees
due
ease
application,
comparatively
lower
cost,
practicality
beekeepers
terms
laboratory
preparation.
Therapeutic Advances in Infectious Disease,
Год журнала:
2025,
Номер
12
Опубликована: Янв. 1, 2025
Lower
respiratory
tract
infections
(LRTIs)
pose
a
significant
threat
to
global
health,
causing
more
than
2
million
deaths
worldwide.
This
menace
is
intensified
by
the
alarming
increase
in
drug
resistance,
which
limits
availability
of
effective
antibiotics
for
bacterial
infections.
Consequently,
there
an
urgent
demand
alternative
therapeutic
options.
Phage
therapy
(PT)
has
re-emerged
as
promising
approach
and
adjunct
antibiotic
treatment.
systematic
review
synthesises
application
PT
LRTIs
humans,
providing
unified
updated
data
on
evaluation
safety
efficacy
LRTIs.
Systematic
review.
Following
PRISMA
guidelines,
comprehensive
search
strategy
was
carried
out
(spanning
January
2000
-
February
2024)
four
databases:
PubMed,
Scopus,
ScienceDirect
Web
Science
retrieve
published
records
humans
only.
The
reference
list
each
included
study
evaluated
possible
inclusion
other
relevant
articles.
Among
18
that
fulfilled
criteria,
70
patients
were
administered
PT.
Microbiologically,
71.42%
(n
=
50/70)
improved;
with
either
eradication
pathogen
or
decrease
load,
whilst
15.71%
11/70)
did
not
record
any
improvement.
About
5.71%
4/70)
recorded
partial/incomplete
improvement,
7.14%
5/70)
microbiological
outcomes
unspecified.
Clinically,
up
74.29%
52/70)
improved,
10.00%
7/70)
showed
no
Another
2.86%
2/70)
12.86%
9/70)
uncategorized.
titres
yielded
positive
ranged
from
105
1012
PFU/mL.
Studies
achieved
substantial
phage
titre
at
site
infection
frequently
observed
notable
improvements.
Regarding
PT,
77.78%
(N
14/18)
studies
adverse
effects
after
administered,
16.66%
3/18)
reported
effects.
Based
recently
originating
mainly
observational
studies,
shown
considerable
treatment
However,
lack
uniform
methodologies
protocols
across
different
cases
management
need
additional
clinical
establish
standardised
pharmacokinetic
elements
overall
protocol
By
doing
so,
we
can
fully
unlock
potential
effectively
managing
infections,
including
Antibiotics,
Год журнала:
2025,
Номер
14(6), С. 545 - 545
Опубликована: Май 27, 2025
Background:
In
response
to
the
urgent
need
for
new
antibiotics
targeting
high-priority
MDR
pathogens,
bacteriophages
(phages)
have
emerged
as
promising
non-traditional
antimicrobial
agents.
Phages
are
viruses
that
infect
bacteria
and
induce
cell
lysis
through
mechanisms
distinct
from
those
of
antibiotics,
making
them
largely
unaffected
by
most
antibiotic
resistance
mechanisms.
Importantly,
phages
been
shown
work
cooperatively
with
an
array
clinically
useful
phage–antibiotic
synergy
(PAS)
represents
a
sophisticated
strategy
may
improve
treatment
outcomes.
However,
interactions
between
diverse,
ranging
synergistic
antagonistic,
understanding
underlying
these
is
crucial
developing
effective
PAS
treatments.
this
review,
we
summarize
potential
evolutionary
molecular
drive
current
landscape
interactions.
Conclusions:
Towards
development
robust
strategies,
review
in
vitro
methods
assessing
considerations
choosing
employing
candidate
combinations.
Antibiotics,
Год журнала:
2025,
Номер
14(6), С. 553 - 553
Опубликована: Май 29, 2025
The
resurgence
of
phage
therapy
in
Western
societies
has
been
direct
response
to
recent
increases
antimicrobial
resistance
(AMR)
that
have
ravaged
many
societies.
While
as
a
concept
around
for
over
100
years,
it
largely
replaced
by
antibiotics
due
their
relative
ease
use
and
predictability
spectrum
activity.
Now
become
less
reliable
greater
antibiotic
microbiome
disruption,
once
again
viable
promising
alternative,
but
is
not
without
its
challenges.
Much
like
the
development
antibiotics,
with
deployment
therapeutics
there
will
be
simultaneous
need
diagnostics
clinical
laboratory.
This
review
provides
an
overview
current
challenges
widespread
adoption
focus
on
diagnostic
Current
barriers
include
lack
standard
methodology
quality
controls
susceptibility
testing
selection,
absence
phage-antibiotic
synergy
testing,
methods
assay
activity
biofilms.
Additionally,
are
number
lab-specific
administrative
regulatory
including
pharmacokinetic
(PK)
pharmacodynamic
(PD)
assays,
account
changes
phages
after
passaging,
guidance
what
required
agency
approvals
how
broad
approval
apply,
increased
lab
personnel
or
automation
work
large
libraries
against
bacteria
isolates.
In
this
review
we
aim
to
address
the
current
status
of
phage
therapy
in
management
multidrug-resistant
infections,
from
compassionate
use
cases
ongoing
clinical
trials,
as
well
challenges
approach
presents
particular
context
CF
patients.
Pathogens,
Год журнала:
2024,
Номер
13(10), С. 896 - 896
Опубликована: Окт. 14, 2024
The
continued
rise
in
antimicrobial
resistance
poses
a
serious
threat
to
public
health
worldwide.
use
of
phages
that
can
have
bactericidal
activity
without
disrupting
the
normal
flora
represents
promising
alternative
treatment
method.
This
practice
has
been
successfully
applied
for
decades,
mainly
Eastern
Europe,
and
recently
used
as
an
emergency
therapy
compassionate
care
United
States.
Here,
we
provide
comprehensive
review
pre-clinical
clinical
applications
phage
concerning
three
major
Gram-negative
pathogens:
PHAGE,
Год журнала:
2024,
Номер
5(4), С. 186 - 202
Опубликована: Окт. 24, 2024
The
escalating
global
threat
of
antibiotic
resistance
has
prompted
a
critical
need
for
innovative
approaches
to
bacterial
infection
treatment.
In
terms
management,
bacterial-associated
disorders
have
reached
point
in
the
world
due
advent
drug-resistant
types
bacteria.
Nonetheless,
continued
bacteriophage
research
presents
promising
frontier
battle
against
infections.
this
study,
we
explored
effectiveness
therapy
antibiotic-resistant
According
our
viewpoint,
phage
could
replace
antibiotics
safety,
efficacy,
and
specificity.
Furthermore,
offers
versatility
administering
single
phage,
products,
or
modified
various
resistant
Moreover,
Phage
cocktails,
with
their
synergistic
combinations,
showcase
holistic
approach,
mitigating
risk
expanding
treatment
possibilities.
Similarly,
synergy
between
phages
holds
promise
overcoming
forging
path
toward
more
effective
sustainable
antimicrobial
strategies.
review
provides
insights
into
transformative
impact
on
current
paradigms,
its
application
methodological
challenges
limitations,
as
well
scientists
policymakers
best
areas
study
order
combat
resistance.