Temperature‐smart plants: A new horizon with omics‐driven plant breeding

Physiologia Plantarum, Год журнала: 2024, Номер 176(1)

Опубликована: Янв. 1, 2024

Abstract The adverse effects of mounting environmental challenges, including extreme temperatures, threaten the global food supply due to their impact on plant growth and productivity. Temperature extremes disrupt genetics, leading significant issues eventually damaging phenotypes. Plants have developed complex signaling networks respond tolerate temperature stimuli, genetic, physiological, biochemical, molecular adaptations. In recent decades, omics tools other strategies rapidly advanced, offering crucial insights a wealth information about how plants adapt stress. This review explores potential an integrated omics‐driven approach understanding temperatures. By leveraging cutting‐edge methods, genomics, transcriptomics, proteomics, metabolomics, miRNAomics, epigenomics, phenomics, ionomics, alongside power machine learning speed breeding data, we can revolutionize practices. These advanced techniques offer promising pathway developing climate‐proof varieties that withstand fluctuations, addressing increasing demand for high‐quality in face changing climate.

Язык: Английский

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Coping with the cold: unveiling cryoprotectants, molecular signaling pathways, and strategies for cold stress resilience

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Авг. 15, 2023

Low temperature stress significantly threatens crop productivity and economic sustainability. Plants counter this by deploying advanced molecular mechanisms to perceive respond cold stress. Transmembrane proteins initiate these responses, triggering a series of events involving secondary messengers such as calcium ions (Ca2+), reactive oxygen species (ROS), inositol phosphates. Of these, signaling is paramount, activating downstream phosphorylation cascades the transcription cold-responsive genes, including cold-regulated (COR) genes. This review focuses on how plants manage freeze-induced damage through dual strategies: tolerance avoidance. Tolerance involve acclimatization decreasing temperatures, fostering gradual accumulation resistance. In contrast, avoidance rely cryoprotectant molecules like potassium (K+), proline, glycerol, antifreeze (AFPs). Cryoprotectants modulate intracellular solute concentration, lower freezing point, inhibit ice formation, preserve plasma membrane fluidity. Additionally, demonstrate antioxidant activity, scavenging ROS, preventing protein denaturation, subsequently mitigating cellular damage. By forming extensive hydrogen bonds with water molecules, cryoprotectants also limit intercellular movement, minimizing extracellular crystal cell dehydration. The deployment key adaptive strategy that bolsters plant resilience promotes survival in environments. However, specific physiological underlying protective effects remain insufficiently understood. Therefore, underscores need for further research elucidate assess their potential impact sustainability, contributing progressive discourse biology environmental science.

Язык: Английский

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Integrated analysis of transcriptomics and metabolomics of peach under cold stress

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Март 27, 2023

Low temperature is one of the environmental factors that restrict growth and geographical distribution peach ( Prunus persica L. Batsch). To explore molecular mechanisms brunches in response to cold, we analyzed metabolomics transcriptomics ‘Donghe No.1’ (cold-tolerant, CT) ‘21 st Century’ (cold-sensitive, CS) treated by different temperatures (-5 -30°C) for 12 h. Some cold-responsive metabolites (e.g., saccharides, phenolic acids flavones) were identified with upregulation only CT. Further, 1991 cold tolerance associated genes these samples they significantly enriched pathways ‘galactose metabolism’, ‘phenylpropanoid biosynthesis’ ‘flavonoids biosynthesis’. Weighted gene correlation network analysis showed soluble sugar, flavone, lignin biosynthetic might play a key role peach. In addition, several COMT, CCR, CAD, PER F3’H) substantially expressed more CT than CS under stress, indicating be major during adaptation low temperature. This study will not improve our understanding towards trees stress but also contribute screening breeding program future.

Язык: Английский

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Transcriptome and Metabolome Analyses Reveal Molecular Responses of Two Pepper (Capsicum annuum L.) Cultivars to Cold Stress

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Март 22, 2022

Low temperature is a significant factor affecting field-grown pepper. The molecular mechanisms behind peppers' response to cold stress remain unknown. Transcriptomic and metabolomic analyses were used investigate the responses of two pepper cultivars, XS (cold-sensitive) GZ (cold-resistant), stress; these screened from 45 materials. In this study, compared with control group (0 h), we identified 10,931 differentially expressed genes (DEGs) in GZ, 657 metabolites (DEMs) positive ion mode, 390 DEMs negative mode. Most DEGs involved amino acid biosynthesis, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signaling pathway. Furthermore, analysis revealed that content free polyamines (PAs), hormones, osmolytes, mainly contained increased putrescine, spermine, spermidine, abscisic (ABA), jasmonic (JA), raffinose, proline, stress. Importantly, regulation ICE (inducer CBF expression)-CBF (C repeat binding factors)-COR (cold regulated) pathway by Ca2+ signaling, MAPK reactive oxygen species (ROS) plays key role regulating peppers Above all, results present study provide important insights into stress, which will reveal potential contribute screening breeding future.

Язык: Английский

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Integrated Transcriptomic and Metabolomics Analyses Reveal Molecular Responses to Cold Stress in Coconut (Cocos nucifera L.) Seedlings

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(19), С. 14563 - 14563

Опубликована: Сен. 26, 2023

Coconut is an important tropical and subtropical fruit oil crop severely affected by cold temperature, limiting its distribution application. Thus, studying low-temperature reaction mechanism required to expand cultivation range. We used growth morphology physiological analyses characterize the response of coconuts 10, 20, 30 d low temperatures, combined with transcriptome metabolome analysis. Low-temperature treatment significantly reduced plant height dry weight coconut seedlings. The contents soil analyzer development (SPAD), soluble sugar (SS), protein (SP), proline (Pro), malondialdehyde (MDA) in leaves were increased, along activities superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), endogenous hormones abscisic acid (ABA), auxin (IAA), zeatin (ZR), gibberellin (GA) contents. A large number differentially expressed genes (DEGs) (9968) detected under conditions. Most DEGs involved mitogen-activated kinase (MAPK) signaling pathway-plant, hormone signal transduction, plant-pathogen interaction, biosynthesis amino acids, nucleotide metabolism, carbon starch sucrose purine phenylpropanoid pathways. Transcription factors (TFs), including WRKY, AP2/ERF, HSF, bZIP, MYB, bHLH families, induced express stress. In addition, most associated major cold-tolerance pathways, such as ICE-CBF-COR, MAPK signaling, their up-regulated. Under a total 205 accumulated metabolites (DAMs) enriched; 206 DAMs positive-ion mode 97 negative-ion mode, mainly phenylpropanoids polyketides, lipids lipid-like molecules, benzenoids, organoheterocyclic compounds, organic oxygen acids derivatives, nucleosides, nucleotides, analogues. Comprehensive analysis revealed that related enriched acid, flavonoid, carbohydrate, lipid, metabolism pathways Together, results this study provide insights into stress, which will reveal underlying molecular mechanisms help screening breeding.

Язык: Английский

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A structural variation in the promoter of the leucoanthocyanidin reductase gene AaLAR1 enhances freezing tolerance by modulating proanthocyanidin accumulation in kiwifruit (Actinidia arguta)

Plant Cell & Environment, Год журнала: 2024, Номер 47(10), С. 4048 - 4066

Опубликована: Июнь 17, 2024

Abstract Proanthocyanidins (PAs) are important metabolites that enhance freezing tolerance of plants. Actinidia arguta , especially freezing‐tolerant germplasms, accumulate abundant PAs in dormant shoots and thereby tolerance, but the underlying mechanism is unknown. In this study, we used two A. with contrasting cold‐resistant phenotypes, KL RB, to explore mechanisms response cold tolerance. We determined a leucoanthocyanidin reductase gene ( AaLAR1 ) was more highly expressed than freezing‐sensitive RB. Moreover, overexpressing kiwifruit promoted biosynthesis enhanced The promoters various germplasms differ due presence 60‐bp deletion cold‐tolerant genotypes forms functional binding site for MYC‐type transcription factor. Yeast one‐hybrid two‐hybrid, dual‐luciferase reporter, bimolecular fluorescence complementation coimmunoprecipitation assays indicated AaMYC2a binds MYC‐core cis ‐element promoter assistance AaMYB5a, promoting accumulation kiwifruit. conclude variation AaMYC2a–AaMYB5a– module shape . identification key structural offers new target resistance breeding

Язык: Английский

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Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Апрель 10, 2024

Mango (Mangifera indica L.) is grown in Hainan, Guangdong, Yunnan, Sichuan, and Fujian provinces Guanxi autonomous region of China. However, trees growing these areas suffer severe cold stress during winter, which affects the yield. To this regard, data on global metabolome transcriptome profiles leaves are limited. Here, we used combined analyses three mango cultivars with different tolerance, i.e. Jinhuang (J)-tolerant, Tainung (T) Guiremang No. 82 (G)-susceptible, after 24 (LF), 48 (MF) 72 (HF) hours cold.

Язык: Английский

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Integrated transcriptomics and metabolomics analyses reveal key genes and essential metabolic pathways for the acquisition of cold tolerance during dormancy in apple

Environmental and Experimental Botany, Год журнала: 2023, Номер 213, С. 105413 - 105413

Опубликована: Июнь 16, 2023

Low temperature is a primary abiotic stress that significantly affects plant growth and development in the cool regions of Northeast China. It limiting factor global apple industry. Numerous studies have investigated alterations transcriptional metabolism under low-temperature various plants. However, research on role dormancy regulating differences cold tolerance among varieties limited. Therefore, this study conducted comprehensive analysis physiological biochemical indices, transcriptome, metabolome cold-tolerant variety 'Hanfu' (HF) cold-sensitive 'Naganofuji 2′ (CF) during endodormancy ecodormancy. Under treatment, cross-section 1-year-old branch CF exhibited more severe browning than HF did, relative electrolyte leakage value was higher HF. Transcriptomics metabolomics revealed key pathways apples response to stress. Functional enrichment showed hormone signal transduction amino acid metabolism-related were enriched ecodormancy, these considered an important way for respond freezing injury. The galactose pathway only endodormancy, while membrane lipid A gene co-expression network constructed using weighted correlation analysis, six modules with biological significance selected from network. Among them, genes encoding transcription factors such as DREB1C/CBF2, JUB1, CCCH, VIP1 are hub different modules, which can be used candidate explore regulation Overall, findings help elucidate molecular mechanism underlying freeze-tolerance provide novel insights into regulatory involved dormancy.

Язык: Английский

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Flavonoids as key players in cold tolerance: molecular insights and applications in horticultural crops

Horticulture Research, Год журнала: 2025, Номер 12(4)

Опубликована: Янв. 2, 2025

Abstract Cold stress profoundly affects the growth, development, and productivity of horticultural crops. Among diverse strategies plants employ to mitigate adverse effects cold stress, flavonoids have emerged as pivotal components in enhancing plant resilience. This review was written systematically highlight critical role tolerance, aiming address increasing need for sustainable practices under climate stress. We provide a comprehensive overview tolerance crops, emphasizing their biosynthesis pathways, molecular mechanisms, regulatory aspects conditions. discuss how act antioxidants, scavenging reactive oxygen species (ROS) generated during they regulate gene expression by modulating stress-responsive genes pathways. Additionally, we explore application through genetic engineering breeding strategies, offering insights into practical interventions improving crop Despite significant advances, research gap remains understanding precise mechanisms which specific confer resistance, especially across different species. By addressing current knowledge gaps, proposing future directions highlighting implications horticulture, aim advance enhance

Язык: Английский

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Widely Targeted Metabolomics and Transcriptomics Analysis of the Response and Adaptation Mechanisms of Trifolium ambiguum to Low-Temperature Stress

Agronomy, Год журнала: 2025, Номер 15(2), С. 308 - 308

Опубликована: Янв. 26, 2025

Caucasian clover (Trifolium ambiguum M.Bieb.) is a perennial legume known for its exceptional cold tolerance, commonly used in agriculture and ecosystems climates. Given the impact of climate change, enhancing adaptation crucial sustaining agricultural productivity. This study employs metabolomics, transcriptomics, Weighted Gene Co-expression Network Analysis (WGCNA) to investigate molecular mechanisms clover’s response low-temperature stress. Metabolomic analysis showed that stress triggered accumulation fatty acids, amino antioxidants, which are critical maintaining membrane stability antioxidant capacity, thus protecting plant from oxidative damage. Transcriptomic revealed significant upregulation genes involved adaptation, particularly those related defense, lipid repair, signal transduction, including ABA signaling pathway enzymes, thereby improving tolerance. WGCNA identified gene modules closely linked especially acid metabolism, repair. These function synergistically, with coordinated expression resistance. also investigated isoflavonoid biosynthesis under stress, highlighting role capacity Low-temperature induced key enzyme genes, such as Isoflavone Synthase (IFS) Isoflavone-7-O-Glucosyltransferase (IF7GT), promoting metabolite further plant’s adaptation. Overall, this offers novel insights into tolerance provides valuable theoretical support breeding cold-resistant crops

Язык: Английский

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