Dual functionality of Trichoderma: Biocontrol of Sclerotinia sclerotiorum and biostimulant of cotton plants

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Окт. 7, 2022

Microbial crop protection products based on Trichoderma have the ability to display multifunctional roles in plant protection, such as pathogen parasitism, enhance nutrient availability and stimulate growth, these traits can be used overall agronomic performance of a variety crops. In current study, we explored potential two indigenous Brazilian strains (T. asperelloides CMAA 1584 T. lentiforme 1585) for their capability controlling Sclerotinia sclerotiorum, key cotton, growth promotion cotton plants (Gossypium hirsutum). Both were able solubilize mineral phosphorus (CaHPO4), release volatile organic compounds that impaired mycelial S. promote under greenhouse conditions. dual culture, reduced rate number sclerotia formed by sclerotiorum. By treating with conidial suspensions strains, strong inhibition myceliogenic germination was observed, result marked mycoparasitic activity exerted sclerotia. The parasitism over sclerotiorum more effective 1584, whilst biostimulant effects pronounced 1585, which also showed higher capacity phosphate solubilization. Thus, displays efficiency while 1585 is suitable due its plants. Overall, may mixture provide both control this strategy will support growers minimizing use synthetic fertilizers fungicides against white mold

Язык: Английский

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Antimicrobial Potential of Essential Oils from Aromatic Plant Ocimum sp.; A Comparative Biochemical Profiling and In-Silico Analysis

Agronomy, Год журнала: 2022, Номер 12(3), С. 627 - 627

Опубликована: Март 4, 2022

Medicinal and aromatic plants (MAPs) are a rich source of bioactive compounds that immensely important due to their potential use in pharmacological agricultural applications. Here, we have evaluated the antimicrobial activity essential oils (EOs) from three different species Ocimum: O. gratissimum (EO1), tenuiflorum (EO2), sanctum (EO3). The EOs were screened for antibacterial against pathogenic strains Escherichia coli, Enterobacter cloacae methicillin-resistant Staphylococcus aureus (MRSA). EO1 EO3 showed significant growth inhibition tested bacteria. Likewise, all exhibited antifungal broad-spectrum plant fungal pathogen Sclerotinia sclerotiorum causes white-mould disease plants. Moreover, correlates well with antioxidant determined by DPPH free radical scavenging activity. biochemical analysis employing high-performance thin-layer chromatography, gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy, revealed presence distinct phytoconstituents might be responsible differential bioactivity. Furthermore, an in-silico evaluation candidate using molecular docking suggests Altogether, our results clearly show possess promising properties, therefore could utilized as agent.

Язык: Английский

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Plant-beneficial Streptomyces dioscori SF1 potential biocontrol and plant growth promotion in saline soil within the arid and semi-arid areas

Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(27), С. 70194 - 70212

Опубликована: Май 5, 2023

Язык: Английский

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Identification and genomic characterization of Pseudomonas spp. displaying biocontrol activity against Sclerotinia sclerotiorum in lettuce

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 7, 2024

Lettuce is an economically major leafy vegetable that affected by numerous diseases. One of the most devastating diseases lettuce white mold caused Sclerotinia sclerotiorum . Control methods for this fungus are limited due to development genetic resistance commonly used fungicides, large number hosts and long-term survival sclerotia in soil. To elaborate a new more sustainable approach contain pathogen, 1,210 Pseudomonas strains previously isolated from agricultural soils Canada were screened their antagonistic activity against S. Nine showed strong vitro inhibition dual-culture confrontational assays. Whole genome sequencing these revealed affiliation with four phylogenomic subgroups within fluorescens group, namely corrugata , asplenii mandelii protegens The harbor several genes gene clusters involved production secondary metabolites, including mycin-type peptin-type lipopeptides, antibiotics such as brabantamide, which may be inhibitory observed Three also demonstrated significant planta biocontrol abilities pathogen when either inoculated on leaves or growing substrate plants grown pots. They however did not impact populations rhizosphere, suggesting they protect altering fitness virulence rather than directly impeding its growth. These results mark step forward products

Язык: Английский

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The Volatile Organic Compounds of Streptomyces spp.: An In-Depth Analysis of Their Antifungal Properties

Microorganisms, Год журнала: 2023, Номер 11(7), С. 1820 - 1820

Опубликована: Июль 16, 2023

The study of volatile organic compounds (VOCs) has expanded because the growing need to search for new bioactive that could be used as therapeutic alternatives. These small molecules serve signals establish interactions with other nearby organisms in environment. In this work, we evaluated antifungal effect VOCs produced by different Streptomyces spp. This was performed using VOC chamber devices allow free exchange without physical contact between microorganisms or diffusible they produce. Antifungal activity tested against Escovopsis weberi, a fungal pathogen affects ant nest stability, and results showed CS014, CS057, CS131, CS147, CS159, CS207, CS227 inhibit reduce growth their emitted VOCs. A GS-MS analysis volatiles captured activated charcoal suggested these strains synthesize several VOCs, many them presence E. accumulation various determining inhibition effect.

Язык: Английский

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Combining Desirable Traits for a Good Biocontrol Strategy against Sclerotinia sclerotiorum

Microorganisms, Год журнала: 2022, Номер 10(6), С. 1189 - 1189

Опубликована: Июнь 9, 2022

The fungal pathogen Sclerotinia sclerotiorum (Helotiales: Sclerotiniaceae) causes white mold, a disease that leads to substantial losses on wide variety of hosts throughout the world. This economically important fungus affects yield and seed quality, its control mostly relies use environmentally damaging fungicides. review aimed present latest discoveries microorganisms biocontrol mechanisms used against mold. A special focus is put identification desirable traits required for efficient control. better understanding involved conditions their action also essential ensure successful implementation under commercial field conditions. In this review, brief introduction pathogen, cycle, main pathogenicity factors presented, followed by thorough description have so far demonstrated potential mold they achieve Antibiosis, induced systemic resistance, mycoparasitism, hypovirulence are discussed. Finally, based our actual knowledge, best strategies S. likely succeed commercially discussed, including combining particular interest.

Язык: Английский

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Clonostachys rosea: Production by Submerged Culture and Bioactivity Against Sclerotinia sclerotiorum and Bemisia tabaci

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Май 6, 2022

Among the prospective biocontrol agents, saprophytic filamentous fungus Clonostachys rosea is an excellent necrotrophic mycoparasite of numerous plant pathogenic fungi. However, its commercial development has been hampered by mass production difficulties during solid-state fermentation. Conversely, submerged liquid fermentation shortens cultivation time while increasing yields fungal propagules. this method overlooked for C. rosea. In work, we investigated impact pre-culture inoculum on spore two-stage process using rice grains in comparison to traditional parallel, studied manipulating carbon-to-nitrogen (C:N) ratio and nitrogen source, with further optimization a benchtop bioreactor. Additional bioassays included assessing bioactivity water-dispersible microgranules (that contained conidia) against whitefly (Bemisia tabaci biotype B) Sclerotinia sclerotiorum (causal agent white mold). Our results showed maximum concentration 1.1 × 109 conidia/g-dry-matter after 7 days process. The yielded 1.4 conidia/ml medium 50:1 C:N ratio, it also induced microsclerotia (MS) up 1.35 104/ml within 6 10:1 ratio; both media were supplemented dextrose monohydrate soybean meal. batches carried out bioreactor amended meal rendered peak fourth day, corresponding 1.11 4.35 108 colony forming units (CFU)/ml. Following air-drying, conidia from air-dried biomass was estimated at 3.4 1010 conidia/g formulated product upon re-hydration days. Both MS inhibited 100% germination S. sclerotia direct parasitism. exhibited suppressive activity (88% mycoparasitism) early nymphs (76.2% mortality) that LC50 values 3.2 104 CFU/g soil 1.5 107 CFU/ml, respectively. Therefore, culture may offer feasible cost-effective large-scale production, alleviating critical constraints their use providing additional tool management B. sclerotiorum.

Язык: Английский

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mRNA Turnover Protein 4 Is Vital for Fungal Pathogenicity and Response to Oxidative Stress in Sclerotinia sclerotiorum

Pathogens, Год журнала: 2023, Номер 12(2), С. 281 - 281

Опубликована: Фев. 8, 2023

Ribosome assembly factors have been extensively studied in yeast, and their abnormalities may affect the process of ribosomes cause severe damage to cells. However, it is not clear whether mRNA turnover protein 4 (MRT4) functions fungal growth pathogenicity Sclerotinia sclerotiorum. Here, we identified nucleus-located gene SsMRT4 using reverse genetics, found that knockdown resulted retard mycelia complete loss pathogenicity. Furthermore, mrt4 mutants showed almost no appressorium formation oxalic acid production comparing wild-type complementary strains. In addition, abilities ROS elimination resistance oxidative osmotic stresses were also seriously compromised mutants. Overall, our study clarified role S. sclerotiorum, providing new insights into ribosome regulating environmental fungi.

Язык: Английский

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Non-proteinogenic amino acids mitigate oxidative stress and enhance the resistance of common bean plants against Sclerotinia sclerotiorum

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 22, 2024

White mold, caused by the necrotrophic fungus Sclerotinia sclerotiorum , is a challenging disease to common bean cultivation worldwide. In current study, two non-proteinogenic amino acids (NPAAs), γ -aminobutyric acid (GABA) and ß -alanine, were suggested as innovative environmentally acceptable alternatives for more sustainable management of white mold disease. vitro GABA -alanine individually demonstrated potent dose-dependent fungistatic activity effectively impeded radial growth development S. mycelium. Moreover, application or ß-alanine seed treatment followed three root drench applications efficiently decreased severity, stimulated plant growth, boosted content photosynthetic pigments treated -infected plants. Furthermore, although higher levels hydrogen peroxide (H 2 O ), superoxide anion (O •− malondialdehyde (MDA) indicated that infection had markedly triggered oxidative stress in infected plants, exogenous both NPAAs significantly reduced studied indicators. Additionally, increased non-enzymatic (total soluble phenolics flavonoids), well enzymatic (catalase [CAT], peroxidases [POX], polyphenol oxidase [PPO]) antioxidants leaves plants improved their scavenging antioxidant efficiency. Applications also raised proline total Lastly, upregulated antioxidant-related genes PvCAT1 PvCuZnSOD1 PvGR . Collectively, NPAAs, coupled with ability alleviate stress, enhance defenses, stimulate establishes them promising eco-friendly production.

Язык: Английский

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Molecular mechanism of oxalic acid synthesis as virulence factor of Sclerotinia sclerotiorum

Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 134, С. 102412 - 102412

Опубликована: Сен. 11, 2024

Язык: Английский

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