Fungal Metabolomics: A Comprehensive Approach to Understanding Pathogenesis in Humans and Identifying Potential Therapeutics

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 93 - 93

Опубликована: Янв. 24, 2025

Metabolomics has emerged as a transformative tool in the study of microbes, including pathogenic fungi, facilitating identification unique metabolic profiles that elucidate their mechanisms, host interactions, and treatment resistance. This review highlights key applications metabolomics understanding fungal metabolites essential for human virulence, such mycotoxins produced by various species, Aspergillus fumigatus (gliotoxin, fumagillins) Candida species (phenylethyl alcohol, TCA cycle metabolites), secondary contribute to pathogenicity. It also explores adaptations fungi relation drug resistance biofilm formation, revealing alterations pathways during infection, seen C. albicans auris. Furthermore, aids deciphering host–pathogen showcasing how like Cryptococcus neoformans modify metabolism promote survival evade immune responses. The antifungal mechanisms benefited from metabolomic approaches, identifying specific metabolite patterns signify resistance, Candidozyma (Candida) auris, informing new therapeutic strategies. integration with other omics technologies is paving way comprehensive biology pathogenesis. Such multi-omics approaches are crucial discovering targets developing innovative treatments. Thus, purpose this provide an overview revolutionizing our pathogenesis, highlight its potential improving

Язык: Английский

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Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 15, 2024

Pinus taeda is an important forest tree species for plantations because of its rapid growth and high yield oleoresins. Although P. distribute in warm wet southern China, drought, sometime serious long time, often occurs the region. To explore drought tolerance usage beneficial microorganisms, seedlings were planted pots inoculated with root endophytic fungus Serendipita indica finally treated stress 53 d. Metabolome proteome their needles analyzed. The results showed that S. inoculation under caused great changes levels some metabolites needles, especially flavonoids organic acids. Among them, eriocitrin, trans -aconitic acid, vitamin C, uric alpha-ketoglutaric A, stachydrine, coumalic itaconic calceolarioside B, 2-oxoglutaric citric acid upregulated more than three times stress, compared to those non-inoculated stress. KEGG analysis pathways enriched such as flavonoid biosynthesis, ascorbate aldarate metabolism, C5-branched dibasic metabolism. Proteome revealed specific differential proteins. Two proteins, namely, H9X056 H9VDW5, only appeared protein H9VNE7 was 11.0 In addition, increased enrichment water deficient-inducible proteins (such LP3-1, LP3-2, LP3-3, dehydrins) involved ribosomal structures A0A385JF23). Meanwhile, biosynthesis metabolism pathways, mainly including phenylpropanoid cutin, suberine wax 2-oxocarboxylic there positive relationships between accumulation Altogether, our metabolome provided a guideline further study functions related

Язык: Английский

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Physiological responses to drought stress of three pine species and comparative transcriptome analysis of Pinus yunnanensis var. pygmaea

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Март 16, 2024

Abstract Drought stress can significantly affect plant growth, development, and yield. Fewer comparative studies have been conducted between different species of pines, particularly involving Pinus yunnanensis var. pygmaea ( P. ). In this study, the physiological indices, photosynthetic pigment related antioxidant enzyme changes in needles from , elliottii massoniana under drought at 0, 7, 14, 21, 28 35 d, as well 7 days after rehydration, were measured. The PacBio single-molecule real-time (SMRT) Illumina RNA sequencing used to uncover gene expression differences rehydration conditions. results showed that total capacity (TAOC) was higher than elliottii. TAOC a continuous increase trend across all species. Soluble sugar (SS), starch content non-structural carbohydrate (NSC) three pines displayed "W" pattern, declining initially, increasing, then decreasing again. exhibits stronger tolerance greater recovery ability prolonged Through SMRT-seq, 50,979 high-quality transcripts generated, 6,521 SSR 5,561 long non-coding RNAs (LncRNAs) identified. A 2310, 1849, 5271, 5947, 7710, 6854 differentially expressed genes (DEGs) identified compared control (Pp0D) six pair-wise comparisons treatment versus control. bHLH NAC ERF MYB_related C3H transcription factors (TFs) play an important role pygmaea. KEGG enrichment analysis Gene set (GSEA) may respond by enhancing metabolic processes such ABA signaling pathway, alpha-linolenic acid. Weighted co-expression network (WGCNA) revealed GST CAT LEC14B SEC23 associated with activity TAOC. This study provides basis for further research on among coniferous

Язык: Английский

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Metabolomic Analysis of the Response of Haloxylon ammodendron and Haloxylon persicum to Drought

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 9099 - 9099

Опубликована: Май 22, 2023

Haloxylon ammodendron and persicum, as typical desert plants in arid areas, show strong drought tolerance environmental adaptability are therefore ideal model for studying the molecular mechanisms of tolerance. A metabolomic analysis H. persicum their natural environment is lacking, metabolic response to remains unclear. To elucidate at level, a non-targeted metabolomics was carried out herein. Under dry environment, exhibited 296 252 differentially expressed metabolites (DEMs) positive negative ion modes, respectively, whereas 452 354 DEMs were identified modes respectively. The results indicated that responds by increasing content organic nitrogen compounds lignans, neolignans, related compounds, reducing alkaloids derivatives. By contrast, adapts acids derivatives compounds. In addition, improved osmoregulation ability, reactive oxygen species detoxification cell membrane stability regulating key pathways anabolism associated metabolites. This first report on providing foundation further study regulatory under stress.

Язык: Английский

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Metabolomic pathways in food allergy

Pediatric Allergy and Immunology, Год журнала: 2024, Номер 35(5)

Опубликована: Май 1, 2024

Abstract Food allergy (FA) is a widespread issue, affecting as many 10% of the population. Over past two to three decades, prevalence FA has been on rise, particularly in industrialized and westernized countries. complex, multifactorial disease mediated by type 2 immune responses involving environmental genetic factors. However, precise mechanisms remain inadequately understood. Metabolomics potential identify endotypes, which could beneficially promote personalized prevention treatment. A metabolome approach would facilitate identification surrogate metabolite markers reflecting activity prognosis. Here, we present literature overview recent metabolomic studies conducted children with FA. image

Язык: Английский

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Comprehensive Proteomic Analysis of Common Bean (Phaseolus vulgaris L.) Seeds Reveal Shared and Unique Proteins Involved in Terminal Drought Stress Response in Tolerant and Sensitive Genotypes

Biomolecules, Год журнала: 2024, Номер 14(1), С. 109 - 109

Опубликована: Янв. 15, 2024

This study identified proteomic changes in the seeds of two tolerant (SB-DT3 and SB-DT2) sensitive (Merlot Stampede) common bean genotypes response to terminal drought stress. Differentially expressed proteins (DEPs) were abundant susceptible genotype compared line. DEPs associated with starch biosynthesis, protein-chromophore linkage, photosynthesis both genotypes, while a few enriched biological pathways exhibited genotype-specific differences. The uniquely showed related sugar metabolism plant signaling, displayed more involved plant-pathogen interaction, proteasome function, carbohydrate metabolism. linked chaperone signal transduction significantly altered between genotypes. In summary, our analysis revealed conserved that could be used as targets selective breeding developing drought-tolerant

Язык: Английский

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Multi-Omics Elucidates Difference in Accumulation of Bioactive Constituents in Licorice (Glycyrrhiza uralensis) under Drought Stress

Molecules, Год журнала: 2023, Номер 28(20), С. 7042 - 7042

Опубликована: Окт. 12, 2023

Licorice is a frequently applied herb with potential edible and medicinal value based on various flavonoids triterpenes. However, studies detailed flavonoid triterpene metabolism the molecular basis of their biosynthesis in licorice are very limited, especially under drought conditions. In present study, we carried out transcriptome, proteome, metabolome experiments. To ultimately combine three omics for analysis, performed bioinformatics comparison, integrating transcriptome data proteome through Cloud platform, along simplified primary triterpenoids KEGG pathway metabolomic results. The pathways triterpenes enriched at both gene protein levels. Key flavonoid-related genes (PAL, 4CL, CHS, CHI, CYP93C, HIDH, HI4OMT, CYP81E1_7) representative proteins (HIDH, CYP81E1_7, VR) were obtained, which all showed high levels after treatment. Notably, one R2R3-MYB transcription factor (Glyur000237s00014382.1), critical regulator biosynthesis, achieved significant upregulated expression as well. glycyrrhizin, bAS CYP88D6 have been found Most differentially expressed (DEGs) (DEPs) similar patterns positively related to metabolic profiles saponin. We believe that suitable stress may contribute accumulation bioactive constituents licorice, our research provides an insight into genetic study quality breeding this plant.

Язык: Английский

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Transcriptomic and Metabolomic Insights into ABA-Related Genes in Cerasus humilis under Drought Stress

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7635 - 7635

Опубликована: Июль 11, 2024

Cerasus humilis, a small shrub of the genus within Rosaceae family, is native to China and renowned for its highly nutritious medicinal fruits, robust root system, remarkable drought resistance. This study primarily employed association transcriptome metabolome analyses assess changes in abscisic acid (ABA) levels identify key regulatory genes C. humilis subjected varying degrees stress. Notably, we observed distinct alterations transcription factors across different intensities. Specifically, our data indicated noteworthy shifts GATA, MYB, MYC, WRKY, C2H2, bHLH factor families. Furthermore, combined transcriptomic metabolomic investigations demonstrated significant enrichment metabolic pathways, such as ‘Carbon metabolism’, ‘Biosynthesis amino acids’, cofactors’, ‘Phenylpropanoid biosynthesis’, ‘Starch sucrose ‘Plant hormone signal transduction’ under moderate (Mod) or severe (Sev) conditions. A total 11 candidate involved ABA biosynthesis signaling pathways were identified. The down-regulated included secoisolariciresinol dehydrogenase-like PYL2. Conversely, including FAD-dependent urate hydroxylase-like, cytochrome P450 97B2, carotenoid cleavage dioxygenase 4 (CCD4), SnRK2.2, ABI 5-like protein 5, PP2C 51, SnRK2.3, up-regulated Mod Sev lays genetic foundation enhance tolerance provides resources plant engineering breeding efforts.

Язык: Английский

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Association between agronomic traits and metabolite profiles on yield response and water use efficiency in newly developed wheat populations under drought stressed conditions

Acta Agriculturae Scandinavica Section B - Soil & Plant Science, Год журнала: 2025, Номер 75(1)

Опубликована: Янв. 29, 2025

Язык: Английский

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Metabolomic Insights into Salt Adaptation in Halophytes

Sustainable development and biodiversity, Год журнала: 2025, Номер unknown, С. 295 - 315

Опубликована: Янв. 1, 2025

Язык: Английский

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