CYTOLOGIA,
Год журнала:
2023,
Номер
88(4), С. 283 - 288
Опубликована: Дек. 24, 2023
Gene
editing
technology
based
on
the
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
system
has
garnered
widespread
use
in
plant
genomes
because
of
its
cost-effectiveness,
efficiency,
and
simplicity.
To
avoid
integration
foreign
genes
any
DNA
fragments
into
target
cell
genomes,
researchers
have
developed
a
that
introduces
vitro-assembled
ribonucleoproteins
(RNPs)
consisting
guide
RNA
(gRNA)
Cas
protein
cells,
enabling
direct
genome
editing.
This
was
designed
to
deliver
RNPs
through
four
distinct
methods:
polyethylene
glycol
(PEG)-mediated
transfection,
particle
bombardment,
electroporation,
lipid
transfection.
In
recent
years,
CRISPR
been
extensively
applied
for
genetic
modification
plants,
providing
strategic
response
environmental
challenges.
Researchers
successfully
established
RNP
systems
various
species.
Despite
some
remaining
issues,
still
shows
significant
promise
future
applications
production
non-genetically
modified
(non-GM)
crops.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 23, 2024
An
increasing
population,
climate
change,
and
diminishing
natural
resources
present
severe
threats
to
global
food
security,
with
traditional
breeding
genetic
engineering
methods
often
falling
short
in
addressing
these
rapidly
evolving
challenges.
CRISPR/Cas
systems
have
emerged
as
revolutionary
tools
for
precise
modifications
crops,
offering
significant
advancements
resilience,
yield,
nutritional
value,
particularly
staple
crops
like
rice
maize.
This
review
highlights
the
transformative
potential
of
technology,
emphasizing
recent
innovations
such
prime
base
editing,
development
novel
CRISPR-associated
proteins,
which
significantly
improved
specificity,
efficiency,
scope
genome
editing
agriculture.
These
enable
targeted
that
enhance
tolerance
abiotic
stresses
well
biotic
stresses.
Additionally,
plays
a
crucial
role
improving
crop
yield
quality
by
enhancing
photosynthetic
nutrient
uptake,
resistance
lodging,
while
also
taste,
texture,
shelf
life,
content
through
biofortification.
Despite
challenges
off-target
effects,
need
more
efficient
delivery
methods,
ethical
regulatory
concerns,
underscores
importance
security
sustainability
It
calls
continued
research
integration
CRISPR
other
emerging
technologies
nanotechnology,
synthetic
biology,
machine
learning
fully
realize
its
developing
resilient,
productive,
sustainable
agricultural
systems.
Frontiers in Genome Editing,
Год журнала:
2023,
Номер
5
Опубликована: Июль 20, 2023
Increased
understanding
of
plant
genetics
and
the
development
powerful
easier-to-use
gene
editing
tools
over
past
century
have
revolutionized
humankind's
ability
to
deliver
precise
genotypes
in
crops.
Plant
transformation
techniques
are
well
developed
for
making
transgenic
varieties
certain
crops
model
organisms,
yet
reagent
delivery
regeneration
remain
key
bottlenecks
applying
technology
most
Typical
protocols
produce
transgenic,
genetically
modified
(GM)
rely
on
transgenes,
chemical
selection,
tissue
culture.
make
edited
(GE)
also
use
even
though
these
may
be
undesirable
final
crop
product.
In
some
crops,
transgenes
routinely
segregated
away
during
meiosis
by
performing
crosses,
thus
only
a
minor
concern.
other
particularly
those
propagated
vegetatively,
complex
hybrids,
or
with
long
generation
times,
such
crosses
impractical
impossible.
This
review
highlights
diverse
strategies
CRISPR/Cas
reagents
regenerable
cells
recover
plants
without
unwanted
integration
transgenes.
Some
examples
include
delivering
DNA-free
as
ribonucleoproteins
mRNA,
relying
expression
from
non-integrated
DNA,
using
novel
mechanisms
viruses
nanoparticles,
unconventional
selection
methods
avoid
and/or
avoiding
culture
altogether.
These
advancing
rapidly
already
enabling
scientists
precision
CRISPR
tools.
Frontiers in Forests and Global Change,
Год журнала:
2025,
Номер
8
Опубликована: Фев. 5, 2025
Climate
change
is
severely
impacting
global
forest
ecosystems,
stressing
woody
plants
due
to
rising
temperatures,
shifting
precipitation
patterns,
and
extreme
weather
events.
These
pressures
threaten
biodiversity
disrupt
the
essential
roles
forests
play
in
carbon
sequestration,
timber
production,
ecosystem
stability.
Traditional
management
strategies,
such
as
selective
breeding,
cannot
keep
up
with
rapid
pace
of
climate
change,
given
long
juvenile
phase
trees.
Multiplex
genome
editing,
particularly
through
CRISPR
technologies,
offers
a
promising
solution
accelerate
development
climate-resilient
traits
plants.
By
simultaneously
targeting
multiple
genes,
multiplex
enables
efficient
modification
polygenic
that
govern
stress
tolerance,
disease
resistance,
other
crucial
resilience
factors.
This
mini-review
examines
potential
technologies
management,
agroecological
practices,
showing
how
they
can
improve
tree
support
sustainable
forestry
response
growing
challenges
change.
Frontiers in Genome Editing,
Год журнала:
2025,
Номер
7
Опубликована: Фев. 25, 2025
Wheat
is
cultivated
across
diverse
global
environments,
and
its
productivity
significantly
impacted
by
various
biotic
stresses,
most
importantly
but
not
limited
to
rust
diseases,
Fusarium
head
blight,
wheat
blast,
powdery
mildew.
The
genetic
diversity
of
modern
cultivars
has
been
eroded
domestication
selection,
increasing
their
vulnerability
stress
due
uniformity.
rapid
spread
new
highly
virulent
aggressive
pathogen
strains
exacerbated
this
situation.
Three
strategies
can
be
used
for
enhancing
disease
resistance
through
genome
editing:
introducing
(R)
gene-mediated
resistance,
engineering
nucleotide-binding
leucine-rich
repeat
receptors
(NLRs),
manipulating
susceptibility
(S)
genes
stop
pathogens
from
exploiting
these
factors
support
infection.
Utilizing
R
the
common
strategy
traditional
breeding
approaches,
continuous
evolution
effectors
eventually
overcome
resistance.
Moreover,
modifying
S
confer
pleiotropic
effects
that
hinder
use
in
agriculture.
Enhancing
paramount
sustainable
production
food
security,
tools
are
great
importance
research
community.
application
CRISPR-based
editing
provides
promise
improve
allowing
access
a
broader
range
solutions
beyond
random
mutagenesis
or
intraspecific
variation,
unlocking
ways
crops,
speeding
up
breeding.
Here,
we
first
summarize
major
context
important
diseases
limitations.
Next,
turn
our
attention
powerful
applications
technology
creating
varieties
against
diseases.
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Ноя. 13, 2023
The
use
of
CRISPR/Cas9
is
currently
the
method
choice
for
precise
genome
engineering
in
plants,
including
biomass
crop
poplar.
most
commonly
used
delivering
and
its
components
poplar
via
Agrobacterium-mediated
transformation,
that
besides
desired
gene-editing
event
also
results
stable
T-DNA
integration.
Here
we
explore
delivery
reagents
DNA-coated
microparticle
bombardment
into
model
tree
Populus
tremula
x
P.
alba
to
evaluate
potential
developing
transgene-free,
gene-edited
trees,
as
well
integrating
donor
DNA
at
specific
target
sites.
Using
an
optimized
transformation
method,
which
favors
regeneration
plants
transiently
express
genes
on
delivered
DNA,
regenerated
are
free
Cas9
antibiotic
resistance-encoding
transgenes.
In
addition,
report
frequent
integration
fragments
Cas9-induced
double-strand
break,
opening
opportunities
toward
targeted
gene
insertions.
Genes,
Год журнала:
2022,
Номер
13(7), С. 1180 - 1180
Опубликована: Июнь 30, 2022
Discovery
of
the
CRISPR-Cas9
gene
editing
system
revolutionized
field
plant
genomics.
Despite
advantages
in
ease
designing
gRNA
and
low
cost
system,
there
are
still
hurdles
to
overcome
mutation
efficiencies,
specifically
hexaploid
wheat.
In
conjunction
with
delivery
transformation
frequency,
efficiency
bottleneck
has
potential
slow
down
advancements
genomic
this
study,
nine
bombardment
parameter
combinations
using
three
gold
particle
sizes
rupture
disk
pressures
were
tested
establish
optimal
stable
frequencies
Utilizing
best
protocol
a
knockout
cassette
phytoene
desaturase
gene,
we
subjected
transformed
embryos
four
temperature
treatments
compared
efficiencies.
The
use
0.6
μm
particles
for
increased
across
all
pressures.
A
heat
treatment
34
°C
24
h
resulted
highest
no
or
minimal
reduction
frequency.
produced
two
M0
mutant
events
albino
phenotypes,
requiring
biallelic
mutations
genomes
parameters
greatly
increases
can
help
advance
research
efforts
wheat