Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 15, 2024

Pinus taeda is an important forest tree species for plantations because of its rapid growth and high yield oleoresins. Although P. distribute in warm wet southern China, drought, sometime serious long time, often occurs the region. To explore drought tolerance usage beneficial microorganisms, seedlings were planted pots inoculated with root endophytic fungus Serendipita indica finally treated stress 53 d. Metabolome proteome their needles analyzed. The results showed that S. inoculation under caused great changes levels some metabolites needles, especially flavonoids organic acids. Among them, eriocitrin, trans -aconitic acid, vitamin C, uric alpha-ketoglutaric A, stachydrine, coumalic itaconic calceolarioside B, 2-oxoglutaric citric acid upregulated more than three times stress, compared to those non-inoculated stress. KEGG analysis pathways enriched such as flavonoid biosynthesis, ascorbate aldarate metabolism, C5-branched dibasic metabolism. Proteome revealed specific differential proteins. Two proteins, namely, H9X056 H9VDW5, only appeared protein H9VNE7 was 11.0 In addition, increased enrichment water deficient-inducible proteins (such LP3-1, LP3-2, LP3-3, dehydrins) involved ribosomal structures A0A385JF23). Meanwhile, biosynthesis metabolism pathways, mainly including phenylpropanoid cutin, suberine wax 2-oxocarboxylic there positive relationships between accumulation Altogether, our metabolome provided a guideline further study functions related

Язык: Английский

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Beneficial endophytic fungi improve the yield and quality of Salvia miltiorrhiza by performing different ecological functions

PeerJ, Год журнала: 2024, Номер 12, С. e16959 - e16959

Опубликована: Фев. 22, 2024

Endophytic fungi can enhance the growth and synthesis of secondary metabolites in medicinal plants.

Язык: Английский

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Significance of Arbuscular Mycorrhizal Fungi in Mitigating Abiotic Environmental Stress in Medicinal and Aromatic Plants: A Review

Foods, Год журнала: 2022, Номер 11(17), С. 2591 - 2591

Опубликована: Авг. 26, 2022

Medicinal and aromatic plants (MAPs) have been used worldwide for thousands of years play a critical role in traditional medicines, cosmetics, food industries. In recent years, the cultivation MAPs has become great interest due to increased demand natural products, particular essential oils (EOs). Climate change exacerbated effects abiotic stresses on growth, productivity, quality MAPs. Hence, there is need eco-friendly agricultural strategies enhance plant growth productivity. Among adaptive by cope with adverse including water stress, salinity, pollution, etc., their association beneficial microorganisms such as arbuscular mycorrhizal fungi (AMF) can improve MAPs' tolerance these stresses. The current review (1) summarizes effect major yield, composition EOs distilled from MAP species; (2) reports mechanisms through which AMF root colonization trigger response at morphological, physiological, molecular levels; (3) discusses contribution synergistic other amendments (e.g., growth-promoting bacteria, organic or inorganic amendments) stressed environments. conclusion, several perspectives are suggested promote future investigations.

Язык: Английский

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The diversity of endophytic fungi in Tartary buckwheat (Fagopyrum tataricum) and its correlation with flavonoids and phenotypic traits

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 14, 2024

Tartary buckwheat (

Язык: Английский

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Resveratrol biosynthesis, optimization, induction, bio-transformation and bio-degradation in mycoendophytes

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Окт. 11, 2022

Resveratrol (3,4,5-trihydroxystilbene) is a naturally occurring polyphenolic stilbene compound produced by certain plant species in response to biotic and abiotic factors. has sparked lot of interest due its unique structure approved therapeutic properties for the prevention treatment many diseases such as neurological disease, cardiovascular diabetes, inflammation, cancer, Alzheimer’s disease. Over last few decades, studies have focused on production resveratrol from various natural sources optimization large-scale production. Endophytic fungi isolated types grapevines Polygonum cuspidatum , primary resveratrol, demonstrated intriguing resveratrol-producing ability. Due increasing demand one active area research use endophytic metabolic engineering techniques resveratrol’s The current review addresses an overview source production, well biosynthesis pathways relevant genes incorporated biosynthesis. Various approaches optimizing fungi, their bio-transformation bio-degradation, are explained detail.

Язык: Английский

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Influence of sugar metabolism on the dialogue between arbuscular mycorrhizal fungi and plants

Horticulture Advances, Год журнала: 2023, Номер 1(1)

Опубликована: Июнь 6, 2023

Abstract Arbuscular mycorrhizal (AM) symbioses in plants are broadly significant because of their capacity to facilitate water and nutrient acquisition thus, promote vigorous growth development among host plants. Many horticultural plants, especially citrus highly dependent on AM fungi. In return, fungi receive sugars lipids from The (mainly sucrose) transported the aerial parts roots form a carbon pool. roots, sucrose is converted hexoses, which then taken up by fungal intraradical hyphae trehalose glycogen for storage extraradical potentially participate soil cycle through as glomalin-related proteins. Therefore, root pool influences sugar metabolism providing guarantee maintain better plant growth, stress resistance, fruit quality, lateral development, sequestration. Thus, player dialog between This review briefly summarizes processes synthesis, transport, metabolism, highlights relationship with an emphasis AM-carbon pools, osmotic adjustment, sugar-associated gene expression. Future research prospects discussed.

Язык: Английский

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Arbuscular mycorrhizal fungi and phosphorus supply accelerate main medicinal component production of Polygonum cuspidatum

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Сен. 8, 2022

The medicinal plant Polygonum cuspidatum Sieb. Et Zucc is rich in stilbenes (e.g., polygonin and resveratrol) anthraquinones emodin) for the therapy of human diseases, while how to increase growth composition concentrations P . has become an urgent issue. aim present study was evaluate effects inoculation with arbuscular mycorrhizal (AM) fungus, Funneliformis mosseae , on growth, phosphorus (P) acquisition, component concentrations, expressions resveratrol synthesis-associated enzyme genes at two levels (0 M 0.2 M). supply (0.2 M) stimulated root AM fungal colonization rate. F significantly improved performance (height, diameter, biomass) morphology (diameter, length, projected area), irrespectively substrate levels. distinctly increased soil acid neutral phosphatase activities, as well concentrations. physcion inoculated uninoculated plants, along up-regulated PcCHS1 PcCRS1 PcRS11 PcSTS plants represented higher aloe-emodin, chrysophanol, emodin, physcion, polydatin, than non-AM irrespective levels, coupled PcCHS2 PcRS It concluded that promoted associated related genes.

Язык: Английский

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Combined Metabolome and Transcriptome Analyses Reveal the Pivotal Role of Mycorrhizal Fungi Tulasnella sp. BJ1 in the Growth and Accumulation of Secondary Metabolites in Bletilla striata (Thunb.) Reiehb.f.

Fungal Biology, Год журнала: 2025, Номер 129(3), С. 101553 - 101553

Опубликована: Март 1, 2025

Язык: Английский

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Soil organic amendments with Polygonum cuspidatum residues enhance growth, leaf gas exchange, and bioactive component levels

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Апрель 25, 2025

The extracted residue of Polygonum cuspidatum (a valuable medicinal plant) rhizome is discarded as waste, while it unclear whether returning this to the field would be beneficial for growth and its active component production P . This study aimed investigate effects applying residues (PRs) on plant growth, photosynthetic activities, root indole-3-acetic acid (IAA) zeatin riboside (ZR) levels, (polydatin, resveratrol, emodin) contents, expression resveratrol-associated genes ( PcRS PcPKS1 ) in plants. experiment comprised four treatments, namely, application potassium sulfate compound fertilizer at a rate 50 kg/667 m 2 PRs rates 1500 (PR1500), 2500 (PR2500), 4000 (PR4000), along with control (CK) receiving no additional substances. Two years later, both PR treatments led substantial increases height, stem diameter, leaf number, number nodes main stems, aboveground (leaf, branch, stem) biomass production, depending used doses applied. Among them, PR2500 treatment exhibited superior performance. Additionally, these significantly boosted IAA (11.0−41.7%) ZR (17.8−46.0%) demonstrating highest efficacy. Root levels were p < 0.01) positively correlated biomass. All except PR4000, elevated SPAD values, net photosynthesis rate, transpiration intercellular CO concentration leaves, showing most pronounced improvements. Fertilization polydatin (6.6−22.0%), emodin (12.1−43.3%), resveratrol (17.8−69.3%, PR4000) significant up-regulation down-regulation roots. In short, organic amendments like PRs, particularly , can viable alternative traditional fertilizers enhancing making them cornerstone eco-friendly farming practices sustainable agriculture.

Язык: Английский

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Endophytic Fungi Accelerate Leaf Physiological Activity and Resveratrol Accumulation in Polygonum cuspidatum by Up-Regulating Expression of Associated Genes

Agronomy, Год журнала: 2022, Номер 12(5), С. 1220 - 1220

Опубликована: Май 19, 2022

Polygonum cuspidatum Sieb. et Zucc. is a major raw material for the extraction of drugs such as resveratrol, while over-exploitation P. decreases yield and drug components. The purpose this study was to analyze effect inoculation with root endophytic fungi Funneliformis mosseae Piriformospora indica singly or in combination biomass production, physiological activities (e.g., chlorophyll, soluble protein, gas exchange) main medicinal ingredients cuspidatum, accompanied by expression levels associated genes resveratrol biosynthesis. Single co-inoculation significantly improved shoot single F. indica, especially promoted leaf chlorophyll soluble-protein concentrations exchange, including photosynthetic rate, transpiration stomatal conductance, intercellular CO2 concentration. application increased polydatin concentrations, it affected chrysophanol, emodin, physcion complex manner. In addition, induced PcCRS1, PcRS11, PcRS, PcSTS, only up-regulated PcCHS1 PcCHS2. It concluded that accelerated activity, accumulation which up-regulation related gene

Язык: Английский

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