Complete genome analysis of Bacillus velezensis TS5 and its potential as a probiotic strain in mice

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Дек. 6, 2023

Introduction In recent years, a large number of studies have shown that Bacillus velezensis has the potential as an animal feed additive, and its probiotic properties been gradually explored. Methods this study, Illumina NovaSeq PE150 Oxford Nanopore ONT sequencing platforms were used to sequence genome TS5, fiber-degrading strain isolated from Tibetan sheep. To further investigate B. TS5 strain, in vivo experiments conducted using 40 five-week-old male specific pathogen-free C57BL/6J mice. The mice randomly divided into four groups: high fiber diet control group (H group), probiotics (HT low (L (LT group). H HT groups fed high-fiber (30%), while L LT low-fiber (5%). total bacteria amount vegetative forms per mouse was 1 × 10 9 CFU day, given same volume sterile physiological saline daily by gavage, experiment period lasted for 8 weeks. Results complete results showed it contained 3,929,788 nucleotides with GC content 46.50%. encoded 3,873 genes partially related stress resistance, adhesion, antioxidants, well production secondary metabolites, digestive enzymes, other beneficial nutrients. bacterium mainly involved carbohydrate metabolism, amino acid vitamin cofactor biological process, molecular function, revealed KEGG GO databases. tests could improve intestinal enzyme activity, liver antioxidant capacity, small intestine morphology, cecum microbiota structure Conclusion These findings confirmed effects sheep feces provided theoretical basis clinical application development new additives.

Язык: Английский

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Bacillus velezensis: a versatile ally in the battle against phytopathogens—insights and prospects

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Авг. 15, 2024

The escalating interest in Bacillus velezensis as a biocontrol agent arises from its demonstrated efficacy inhibiting both phytopathogenic fungi and bacteria, positioning it promising candidate for biotechnological applications. This mini review aims to offer comprehensive exploration of the multifaceted properties B. velezensis, with particular focus on beneficial interactions plants potential controlling fungi. molecular dialogues involving plants, phytopathogens are scrutinized underscore intricate mechanisms orchestrating these interactions. Additionally, elucidates mode action particularly through cyclic lipopeptides, highlighting their importance promoting plant growth. agricultural applications detailed, showcasing role enhancing crop health productivity while reducing reliance chemical pesticides. Furthermore, extends purview industrial environmental arenas, versatility across various sectors. By addressing challenges such formulation optimization regulatory frameworks, chart course effective utilization velezensis. • fights phytopathogens, boosting biotech shapes agri-biotech future, offers sustainable solutions Explores plant-B. dialogue, lipopeptide showcased

Язык: Английский

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The extracytoplasmic sigma factor σX supports biofilm formation and increases biocontrol efficacy in Bacillus velezensis 118

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 13, 2025

Plant growth promoting rhizobacteria (PGPR) offer an environmentally friendly and sustainable approach to combat pathogens enhance crop production. The biocontrol activity of PGPR depends on their ability colonize plant roots synthesize antimicrobial compounds that inhibit pathogens. However, the regulatory mechanisms underlying these processes remain unclear. In this study, we isolated characterized Bacillus velezensis 118, a soil isolate exhibits potent against Fusarium wilt banana. Deletion sigX, encoding extracytoplasmic function (ECF) sigma factor previously implicated in controlling biofilm architecture B. subtilis, reduced efficacy. 118 sigX mutant displayed formation but had only minor defect swarming motility negligible impact lipopeptide These findings highlight importance important for root colonization effectiveness spp. as agents phytopathogens.

Язык: Английский

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Bacillus velezensis SS-20 as a potential and efficient multifunctional agent in biocontrol, saline-alkaline tolerance, and plant-growth promotion

Applied Soil Ecology, Год журнала: 2024, Номер 205, С. 105772 - 105772

Опубликована: Ноя. 30, 2024

Язык: Английский

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Predicting drought stress under climate change in the Southern Central Highlands of Vietnam

Environmental Monitoring and Assessment, Год журнала: 2024, Номер 196(7)

Опубликована: Июнь 20, 2024

Язык: Английский

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Plant-Associated Representatives of the Bacillus cereus Group Are a Rich Source of Antimicrobial Compounds

Microorganisms, Год журнала: 2023, Номер 11(11), С. 2677 - 2677

Опубликована: Окт. 31, 2023

Seventeen bacterial strains able to suppress plant pathogens have been isolated from healthy Vietnamese crop plants and taxonomically assigned as members of the Bacillus cereus group. In order prove their potential biocontrol agents, we perform a comprehensive analysis that included whole-genome sequencing selected mining for genes gene clusters involved in synthesis endo- exotoxins secondary metabolites, such antimicrobial peptides (AMPs). Kurstakin, thumolycin, other AMPs were detected characterized by different mass spectrometric methods, MALDI-TOF-MS LIFT-MALDI-TOF/TOF fragment analysis. Based on sequences, plant-associated isolates following species subspecies: B. subsp. (6), bombysepticus (5), tropicus (2), pacificus. These three represent novel genomospecies. Genes encoding entomopathogenic crystal vegetative proteins TK1. The vitro assays revealed many enhanced growth suppressed pathogens. Our findings indicate representatives group are rich source putative compounds with sustainable agriculture. However, presence virulence might restrict application biologicals

Язык: Английский

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The extracytoplasmic sigma factor SigX supports biofilm formation and increases biocontrol efficacy in Bacillus velezensis 118

Опубликована: Окт. 3, 2024

Utilizing plant growth promoting rhizobacteria (PGPR) to combat pathogens and enhance crop production is an environmentally friendly sustainable approach. The biocontrol activity of PGPR depends on their ability colonize roots synthesize antimicrobial compounds that inhibit pathogens. However, the regulatory mechanisms underlying these processes remain unclear. In this study, we isolated characterized Bacillus velezensis isolate 118, a soil isolate exhibits potent against Fusarium wilt banana. Deletion sigX, extracytoplasmic function (ECF) sigma factor previously implicated in controlling biofilm architecture in B. subtilis, reduced efficacy. B. 118 sigXmutant displayed reduced formation but had only minor defect swarming motility negligible impact lipopeptide production. These findings highlight importance important for root colonization effectiveness Bacillus spp. as agents phytopathogens.

Язык: Английский

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Plant-Associated Representatives of the <em>Bacillus cereus</em> Group Harbor a Rich Biosynthetic Potential of Antimicrobial Compounds and are Efficient in Suppressing Plant Pathogens

Опубликована: Окт. 10, 2023

Seventeen bacterial strains able to suppress plant pathogens have been isolated from healthy Vietnamese crop plants, and taxonomically assigned as members of the Bacillus cereus group. In order prove their potential biocontrol agents, we performed a comprehensive analysis which included whole genome sequencing selected strains, mining for genes gene clusters involved in synthesis endo- exotoxins, secondary metabolites, such antimicrobial peptides (AMPs). Kurstakin, thumolycin, other AMPs were detected characterized by different mass spectrometric methods, MALDI-TOF-MS, LIFT-MALDI-TOF/TOF fragment analysis. Based on sequences, plant-associated isolates following species subspecies: B. subsp. (6), bombysepticus (5), tropicus (2), pacificus. Three represented novel genomospecies. Genes encoding entomopathogenic crystal vegetative proteins TK1. vitro assays revealed that many enhanced growth suppressed pathogens. Our findings indicated representatives group are rich source putative compounds with sustainable agriculture. However, presence virulence might restrict application biologicals

Язык: Английский

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Investigation of the potential of Brevibacillus spp. for the biosynthesis of nonribosomally produced bioactive compounds by combination of genome mining with MALDI-TOF mass spectrometry

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Дек. 14, 2023

The biosynthetic potential of 11 Brevibacillus spp. strains was investigated by combination genome mining with mass spectrometric analysis using MALDI-TOF spectrometry. These endophytic, plant associated were isolated from crop plants, such as coffee and black pepper, in Vietnam. Draft genomes these available. They classified (a) comparison type a collection genome-sequenced deposited the NCBI data base well (b) construction phylogenetic tree core sequences publicly available strains. identified brevis (1 strain); parabrevis (2 strains); porteri (3 5 novel genomospecies. Our work specifically focused on detection characterization nonribosomal peptides produced Structural compounds performed LIFT-MALDI-TOF/TOF sequence analysis. highlights our demonstration tyrocidines, well-known family cyclodecapeptides great structural variability, main products all identification class pentapeptides B. ; schisleri which we designate brevipentins. studies demonstrate that knowledge their capacity can efficiently assist classification species.

Язык: Английский

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