The application of machine learning approaches to classify and predict fertility rate in Ethiopia

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 20, 2025

Integrating machine learning (ML) models into healthcare systems is a rapidly evolving field with the potential to revolutionize care delivery. This study aimed classify fertility rates and identify significant predictors using ML among reproductive women in Ethiopia. utilized eight 5864 reproductive-age Ethiopian Demographic Health Survey (EDHS), 2019 data. Phyton programming language was used develop these models. Predictors of rate were determined feature important techniques. The performance evaluated accuracy, area under curve (AUC), precision, recall, F1-score, specificity, sensitivity. mean age participants 32.7 (± 5.6) years. random forest classifier (accuracy = 0.901 AUC 0.961) followed by one-dimensional convolutional neural network 0.899 0.958), logistic regression 0.874 0.937), gradient boost 0.851 0.927) top performing Family size, age, occupation, education an average importance score 0.198, 0.151, 0.118, 0.081, respectively rate. best predict forest, network, regression, classifier. findings on factors can inform targeted public health, programs that address disparities related family education, other socioeconomic factors.

Язык: Английский

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Inhibition of CCN5 Protects Against Apoptosis and Endoplasmic Reticulum Stress in Bisphenol A-Induced Sertoli Cells via p38/JNK MAPK Signaling Pathway

DNA and Cell Biology, Год журнала: 2025, Номер 44(4), С. 174 - 185

Опубликована: Апрель 1, 2025

Bisphenol A (BPA) is the most common endocrine disruptor that has toxicity to reproductive system and male infertility. However, underlying mechanisms of BPA's Sertoli cells remain poorly understood. Cellular communication network factor 5 (CCN5) reported regulate cell proliferation, apoptosis, differentiation. Our study demonstrated a significant elevation CCN5 expression in testis nonobstructive azoospermia patients TM4 exposed BPA. Knockdown reduced apoptotic after BPA treatment, as determined by flow cytometry terminal deoxynucleotidyl transferase dUTP nick end labeling assays. Cells showed increased expressions Bax cleaved poly(ADP-ribose) polymerase, decreased Bcl-2, well elevated activities caspase-3 caspase-9 BPA-induced cells, which were reversed inhibition. Loss declined phosphorylation protein kinase R-like endoplasmic reticulum eukaryotic translation initiation 2A activating transcription 4 C/EBP-homologous BPA-treated cells. Furthermore, silencing blocked p38 c-Jun N-terminal (JNK). Administration anisomycin, mitogen-activated (MAPK) activator, effects knockdown on (ER) stress apoptosis. Taken together, promotes apoptosis ER p38/JNK MAPK signaling pathway.

Язык: Английский

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Paternal age, de novo mutations, and offspring health? New directions for an ageing problem

Human Reproduction, Год журнала: 2024, Номер 39(12), С. 2645 - 2654

Опубликована: Окт. 3, 2024

This Directions article examines the mechanisms by which a father's age impacts health and wellbeing of his children. Such are significant include adverse birth outcomes, dominant genetic conditions, neuropsychiatric disorders, variety congenital developmental defects. As well as age, wide environmental lifestyle factors also known to impact offspring via changes mediated male germ line. picture dynamic line responsive range intrinsic extrinsic contrasts with results trio studies indicating that incidence mutations in is low exhibits linear, monotonic increase paternal (∼two new per year). While traditional explanation for this pattern mutation has been metronomic plod replication errors, an alternative model pivots around 'faulty male' hypothesis. According concept, integrity can be dynamically impacted other factors, it aberrant repair such damage drives mutagenesis. Fortunately, DNA proofreading during spermatogenesis extremely effective these mutant cells either repaired or deleted apoptosis/ferroptosis. There appear only two escape apoptotic fate: (i) if acquire enhancing proliferation suppressing apoptosis, permits their clonal expansion (selfish selection hypothesis) (ii) genetically damaged spermatozoon manages fertilize oocyte, then fixes (or epimutation) result defective (oocyte collusion hypothesis). Exploration proposed should not help us better understand aetiology effects but inform potential avenues remediation.

Язык: Английский

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Redox-Driven Epigenetic Modifications in Sperm: Unraveling Paternal Influences on Embryo Development and Transgenerational Health

Antioxidants, Год журнала: 2025, Номер 14(5), С. 570 - 570

Опубликована: Май 9, 2025

Male-factor infertility accounts for nearly half of all cases, and mounting evidence points to oxidative stress as a pivotal driver sperm dysfunction, genetic instability, epigenetic dysregulation. In particular, the DNA lesion 8-hydroxy-2′-deoxyguanosine (8-OHdG) has emerged central mediator at interface damage regulation. We discuss how this can disrupt key mechanisms such methylation, histone modifications, small non-coding RNAs, thereby influencing fertilization outcomes, embryo development, offspring health. propose that interplay between reprogramming is further exacerbated by aging in both paternal maternal germlines, creating “perfect storm” increases risk heritable (epi)mutations. The consequences unresolved lesions thus persist beyond fertilization, contributing transgenerational health risks. Finally, we explore promise potential pitfalls antioxidant therapy strategy mitigate damage. While supplementation may hold significant therapeutic value men with subfertility experiencing elevated stress, careful, personalized approach essential avoid reductive unintended disruptions. Recognizing dual role shaping genome epigenome underscores need integrating redox biology into reproductive medicine, aim improving fertility treatments safeguarding future generations.

Язык: Английский

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Development of an improved medium for the preservation of human spermatozoa

Journal of Assisted Reproduction and Genetics, Год журнала: 2025, Номер unknown

Опубликована: Май 29, 2025

Abstract Purpose To create a novel medium that retained human sperm quality following cryopreservation at higher level than seen with currently available commercial cryoprotectants. Methods Cryopreservation was achieved via 1:1 dilution cryoprotectant followed by slow-programmed freezing. A NaCl-free carrier based on the use of histidine as major osmolyte designed capable sustaining motility over 6 days ambient temperature. This supplemented ethylene glycol, glycerol, and DMSO to basis for medium. Dose-dependent studies various supplements were then conducted optimize effectiveness this formulation including assessments vitamin C, EDTA, crocin, zinc, ergothioneine, myo -inositol, well potential replacement Cyrene™. Post-thaw samples assessed motility, vitality, DNA integrity reassessed isolation Felix™ System. Results The completed comprised 4.5% 1% in 0.4 mM 1 22 -inositol. Spermatozoa frozen isolated using System had significantly greater total progressive spermatozoa commercially product is widely used infertility clinics. Conclusion has been developed study represents significant improvement existing technologies.

Язык: Английский

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10-Hydroxy Decanoic Acid and Zinc Oxide Nanoparticles Retrieve Nrf2/HO-1 and Caspase-3/Bax/Bcl-2 Signaling in Lead-Induced Testicular Toxicity

Biological Trace Element Research, Год журнала: 2024, Номер unknown

Опубликована: Сен. 30, 2024

Язык: Английский

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Crossover recombination between homologous chromosomes in meiosis: recent progress and remaining mysteries

Trends in Genetics, Год журнала: 2024, Номер 41(1), С. 47 - 59

Опубликована: Окт. 25, 2024

Язык: Английский

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Detrimental effects of cadmium on male infertility: A review

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 290, С. 117623 - 117623

Опубликована: Дек. 28, 2024

Язык: Английский

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Socio-Economic and Health Literacy Inequalities as Determinants of Women’s Knowledge about Their Reproductive System: A Cross-Sectional Study

Epidemiologia, Год журнала: 2024, Номер 5(4), С. 627 - 642

Опубликована: Сен. 26, 2024

Background/Objectives: To support women’s informed decisions and reproductive self-care, confident health-related knowledge is needed, supported by adequate health literacy (HL). No corresponding survey has been carried out in Hungary on inequalities to provide information addressing education. Materials Methods: In the current cross-sectional online survey, 301 women of age (27.16 ± 0.36 years) were asked with Hungarian versions validated standardised questionnaires about hormones, ovulation, menstrual cycle, pregnancy signs birth control (Knowledge Female Body Scale—KFB), HL (Brief Health Literacy Screening Tool—BRIEF). Spearman correlation multivariable linear regression analyses utilised, a significance level set at p < 0.05. IBM SPSS version 28.0 (IBM SPSS, Armonk, NY, USA: Corp.) G*Power (version 3.1.9.7; Heinrich-Heine-Universität Düsseldorf, Germany) software. The STROBE checklist was followed. Clinical Trial Registry Nr. NCT06146673. Results: KFB composite score high (20.01 2.33); 86.374% had “high knowledge”. Still, lacking identified for mechanisms certain contraceptive methods early physical pregnancy. A significant difference also found scores case higher (p = 0.019), education 0.018) previous live 0.028). positive between 0.001), 0.005), 0.021). multiple analysis (R2 0.087, 0.001) indicated that both 0.003) are potential predictors knowledge, whereas induced abortion 0.013) might serve as an inverse predictor. Conclusions: Inequalities their system found, it significantly lowest highest conception probability age. Therefore, addition targeted education, needs improvement.

Язык: Английский

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Reproductive toxicology: keeping up with our changing world

Frontiers in Toxicology, Год журнала: 2024, Номер 6

Опубликована: Окт. 11, 2024

Reproductive toxicology testing is essential to safeguard public health of current and future generations. Traditional toxicological male reproduction has focused on evaluating substances for acute toxicity the reproductive system, with fertility assessment as a main endpoint infertility adverse outcome. Newer studies in last few decades have significantly widened our understanding what represents an event toxicology, thus changed perspective constitutes toxicant, such endocrine disrupting chemicals that affect offspring intergenerational manner. Besides or congenital abnormalities, outcomes can present increased likelihood various problems offspring, including metabolic syndrome, neurodevelopmental like autism cancer predisposition, among others. To enable toxicologic accurately represent population, designs need model changing population characteristics exposure circumstances. Current trends increasing importance human include paternal age, associated decline nicotinamide adenine dinucleotide (NAD), higher prevalence obesity, both which are factors study design should account for. In this article, we highlighted some limitations standard protocols, expanding assessed by genetic epigenetic sperm parameters, potential recent developments, mixture testing, novel animal models, vitro systems organoids, multigenerational well silico modelling, machine learning artificial intelligence.

Язык: Английский

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