Precision Engineering of Chondrocyte Microenvironments: Investigating the Optimal Reaction Conditions for Type B Gelatin Methacrylate Hydrogel Matrix for TC28a2 Cells

Journal of Functional Biomaterials, Год журнала: 2024, Номер 15(3), С. 77 - 77

Опубликована: Март 20, 2024

Gelatin methacrylate (GelMA) is a photocrosslinkable biomaterial that has gained widespread use in tissue engineering due to its favorable biological attributes and customizable physical mechanical traits. While GelMA compatible with various cell types, distinct cellular responses are observed within hydrogels. As such, tailoring hydrogels for specific applications become imperative. Thus, our objective was develop tailored enhance viability specifically TC28a2 chondrocytes three-dimensional (3D) culture setting. We investigated synthesis using PBS 0.25M CB buffer, analyzed the traits of hydrogels, evaluated how varying crosslinking conditions (GelMA concentration, photoinitiator UV exposure time) affected chondrocytes. The results revealed buffer led greater degree methacrylation compared LAP demonstrated superior efficacy gelation Irgacure 2959. Additionally, stiffness, porosity, swelling were predominantly by while impacted all conditions, decreasing notably increasing time. This study facilitated optimization critical aspect diverse biomedical applications.

Язык: Английский

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Navigating Neurotoxicity and Safety Assessment of Nanocarriers for Brain Delivery: Evaluation Models and Insights

Acta Biomaterialia, Год журнала: 2024, Номер 189, С. 25 - 56

Опубликована: Сен. 20, 2024

Язык: Английский

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Fabrication and Characterization of Zn Particle Incorporated Fibrous Scaffolds for Potential Application in Tissue Healing and Regeneration

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(42), С. 48913 - 48929

Опубликована: Окт. 17, 2023

Zinc (Zn) metal and its alloys have received a lot of interest in biomedical applications due to their biodegradability, biocompatibility, antimicrobial activity, ability stimulate tissue regeneration. Bulk Zn has been successfully utilized variety implant applications, most notably as bioabsorbable cardiac stents orthopedic fixation devices, where it provides adequate mechanical properties while also releasing helpful ions (Zn2+) during degradation. Such beneficial are dose-dependent and, when released excess, can induce cellular toxicity. In this study, we hypothesize that embedding particles into polymer nanofibrous scaffold will enable control the degradation time release Zn2+. We designed fabricated two scaffolds, polycaprolactone (PCL) polycaprolactone-chitosan (PCL-CH). Each had an increasing amount Zn. Several physicochemical such fiber morphology, crystallinity, strength, hydrophilicity, Zn2+, thermal properties, chemical compositions, so forth were characterized compared with PCL fibrous scaffold. The biological scaffolds evaluated vitro utilizing direct indirect cytotoxicity assays cell viability. All data show addition changed various physical PCL-CH except structure. Further investigation reveals degrade relatively faster than because presence hydrophilic CH influences Zn2+ culture conditions combined advantages enriched 3T3 fibroblast cells' survival proliferation ones higher concentration particles. These new composite promising be further considered for healing regeneration applications.

Язык: Английский

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Data-Driven Quantitative Intrinsic Hazard Criteria for Nanoproduct Development in a Safe-by-Design Paradigm: A Case Study of Silver Nanoforms

ACS Applied Nano Materials, Год журнала: 2023, Номер 6(5), С. 3948 - 3962

Опубликована: Фев. 16, 2023

The current European (EU) policies, that is, the Green Deal, envisage safe and sustainable practices for chemicals, which include nanoforms (NFs), at earliest stages of innovation. A theoretically by design (SSbD) framework has been established from EU collaborative efforts toward definition quantitative criteria in each SSbD dimension, namely, human environmental safety dimension environmental, social, economic sustainability dimensions. In this study, we target demonstrate journey intrinsic hazard derived findable, accessible, interoperable, reusable data. Data were curated merged development new approach methodologies, structure-activity relationship models based on regression classification machine learning algorithms, with intent to predict a class. utilize system (i.e., hydrodynamic size polydispersity index) non-system elemental composition core size)-dependent nanoscale features combination biological vitro attributes experimental conditions various silver NFs, functional antimicrobial textiles, cosmetics applications. second step, interpretable rules (criteria) followed certainty factor obtained exploiting Bayesian network structure crafted expert reasoning. probabilistic model shows predictive capability ≈78% (average accuracy across all classes). work, show how shifted conceptualization realistic implementation pragmatic instances. This study reveals (i) be considered aspects during synthesis stage, (ii) challenges within, (iii) future directions generation distillation such can feed paradigms. Specifically, guide material engineers synthesize NFs are inherently safer alternative nanoformulations, innovation, while enable fast cost-efficient silico toxicological screening previously synthesized hypothetical scenarios yet-to-be NFs.

Язык: Английский

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Nanofibers Loaded Levosalbutamol Sulfate Fast Dissolving Oral Films for Sublingual Delivery

Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 99, С. 105920 - 105920

Опубликована: Июль 3, 2024

Язык: Английский

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In vitro investigation the effects of iodinated contrast media on endothelial cell viability, cell cycle, and apoptosis

Toxicology Mechanisms and Methods, Год журнала: 2024, Номер unknown, С. 1 - 8

Опубликована: Июль 31, 2024

In medical practice, iodine contrast media are necessary for diagnostic techniques. However, it comes with a potential risk to the patient in form of allergic reactions. The aim this research is study effects on endothelial cells an vitro system at various concentrations, specifically investigating their impact cell viability, cycle, and apoptosis treated within field radiology. Our results showed that media, when concentration was range 2.5-50 mgI/ml, viability decreased by 50%. Conversely, exposure ioversol concentrations between 12.5 50.0 mgI/ml resulted notable increase percentage total apoptotic cells, including both early late apoptosis. conclusion, our investigation sheds light effect iodinated cycle progression, These findings contribute valuable insights ensure safety use, aligning guidelines radiological procedures. Further adherence established crucial refining understanding promoting safe application

Язык: Английский

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Anticancer Activity of Rice Callus Suspension Cultures from Aromatic Varieties and Metabolites Regulated in Treated Cancer Cell Lines

Rice Science, Год журнала: 2024, Номер 31(4), С. 449 - 462

Опубликована: Апрель 12, 2024

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints, for cancer prevention. Rice callus suspension cultures (RCSC) seed extracts prepared from aromatic rice varieties evaluated cytotoxic impact on human colon lung cell lines, as well a normal control line, using Taxol positive control. RCSC two Indian applied at different concentrations treat the lines fibroblasts over varying intervals. Apoptosis was assessed in 1:5 dilution A549 HT-29 treated 72 h, propidium iodide staining flow cytometry. showed more potent effect than minimal contrast Taxol. Confocal microscopy cytometry further confirmed apoptotic RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved cytotoxicity, highlighted altered pathways. is proposed an alternative source development novel anticancer drugs reduced effects.

Язык: Английский

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Urolithin A affects cellular migration and modulates matrix metalloproteinase expression in colorectal cancer cells

Cell Biochemistry and Function, Год журнала: 2024, Номер 42(3)

Опубликована: Апрель 1, 2024

Abstract Colorectal cancer (CRC) is the world's second most common gastrointestinal malignancy. Preventing tumor cell proliferation and dissemination critical for patient survival. Polyphenols have a variety of health advantages can help prevent cancer. The current study examined different cellular activities gut‐microbiota metabolite urolithin A (UA) on several colon lines. results revealed that UA suppressed growth in dose‐ time‐dependent manner. In investigation, substantially affected migration wound‐healing experiment greatly decreased number colonies generated each CRC culture. cells 48 h after treatment, which was significant ( p < .001) compared to rate untreated cells. When cells, slowed process colony formation by reducing or altering their morphological shape. western blot analysis investigation inhibits metastasis lowering expression levels matrix metalloproteinases 1 2 (MMP1 MMP2) more than 43% 41% HT29, 28% 149% SW480, 90% 74% SW620, respectively, at 100 µM dosage control. Surprisingly, UA, tissue inhibitor (TIMP1) were elevated SW620 treated with 89%, 57%, 29%, respectively. Our findings imply has anticancer properties might be used therapeutically treat CRC. provided first indication influence All these data showed as an adjuvant therapy treatment various forms

Язык: Английский

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Portable Sensing Probe for Real-Time Quantification of Ammonia in Blood Samples

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(36), С. 47242 - 47256

Опубликована: Авг. 27, 2024

The detection of ammonia levels in blood is critical for diagnosing and monitoring various medical conditions, including liver dysfunction metabolic disorders. However, traditional diagnostic methods are slow cumbersome, often involving multiple contact-based steps such as separation alkali conditions followed by distillation or microdiffusion, leading to delays diagnosis treatment. Herein, we developed a colorimetric assay capable rapid whole plasma samples, utilizing 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO)-oxidized cellulose nanocrystals (TCNC) coupled with gold nanoparticles (AuNPs). basis our relies on either (i) the interaction between carboxylate group (−COO) TEMPO ammonium ions (ii) manipulation AuNPs surface plasmon resonance (SPR) through formation Au(NH3)43+, which displaces redox mediator, resazurin, resulting observable multicolor displays at concentrations ammonia. exhibits wide linear range dissolved NH4+ (0.1–37 μM) low limit (LOD) 0.1 μM. Additionally, it effectively measures NH3(g) 0.5–144 fabricated electrochemical nose (E-nose) device demonstrates excellent analytical performance sensing (0.05–256 μM). Experimental results demonstrate suitable clinical applications, correlation standard laboratory methods, offering practical solution point-of-care (PoC) testing. We anticipate that this approach can be applied broadly improve patient treatment providing immediate accurate measurements setting.

Язык: Английский

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The Influence of Nanoparticles of Graphene Oxide‑PEG on Cytokine Profile of Monocytes from Human Blood In Vitro

Trends in Immunotherapy, Год журнала: 2025, Номер 9(1), С. 8 - 22

Опубликована: Янв. 17, 2025

This study investigated the response of human monocytes to co-culture with pegylated (linear or branched) graphene oxide (GO) nanoparticles, specifically examing both small (P-GOs, 100 -200 nm) and larger (P-GOb, 1-5 μm) particles at concentrations 5, 25, 50 µg mL–1. Human (CD14+ cells) were isolated cultured these nanoparticles for 72 hours. We measured cell viability, lactate dehydrogenase (LDH) release, cytokine production. The findings showed that P-GO had little effect on production, including MIF, GM-CSF, VEGF, IP-10, IL-8, HGF, SCGF-beta in vitro. At a low concentration (5 μg mL–1), exhibited minimal influence cytokines, except forthe LP-GOb variant, which increased M-CSF Conversely, 25 mL–1 enhanced release variouscytokines, proinflammatory IL-6, IL-1β, IL-1α, IL-18, IL-17, IL-16, IFN-γ, TNF-β, TNF-α, anti-inflammatory IL-1ra, IL-13, IL-10, IL-4, regulatory G-CSF, IL-2, IL-3, IL-5, IL-12 (p40), (p70), M-CSF, GM-CSF chemokines CTACK, Eotaxin, GRO-α, RANTES, MIP-1β, MCP-1, MIP-1α, MCP-3, MIG, SDF-1α, growth factors Basic FGF, PDGF-BB, SCF, LIF TRAIL. Although higher resulted significant monocyte viability remained largely unaffected . LDH was elevated solely samples treated LP-GOb. BP-GOs profiles, raising levels highest concentration. These results indicate modifying may hold potential creating graphene-based pharmacological agents.

Язык: Английский

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