C-C Motif Chemokine Ligand 2 Enhances Macrophage Chemotaxis, Osteogenesis, and Angiogenesis during the Inflammatory Phase of Bone Regeneration

Biomolecules, Journal Year: 2023, Volume and Issue: 13(11), P. 1665 - 1665

Published: Nov. 18, 2023

Local cell therapy has recently gained attention for the treatment of joint diseases and fractures. Mesenchymal stem cells (MSCs) are not only involved in osteogenesis angiogenesis, but they also have immunomodulatory functions, such as inducing macrophage migration during bone regeneration via crosstalk. C-C motif chemokine ligand 2 (CCL2), a known inflammatory mediator, is associated with macrophages inflammation. This study examined utility CCL2 therapeutic target local therapy. Using lentiviral vectors rabbit MSCs, genetically modified overexpressing MSCs were generated. Osteogenic differentiation assays performed using or without co-culture, performed. Additionally, co-cultures endothelial (ECs), angiogenesis was evaluated tube formation assay. Overexpression did affect under monoculture conditions promoted chemotaxis when co-cultured macrophages. Furthermore, CCL2-overexpression co-culture ECs. These results suggest that induces by promoting crosstalk between macrophages; stimulates ECs to induce angiogenesis. findings indicate may be useful areas loss.

Language: Английский

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Vitamin B12-loaded chitosan-based nanoparticle-embedded polymeric nanofibers for sublingual and transdermal applications: Two alternative application routes for vitamin B12

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 258, P. 128635 - 128635

Published: Dec. 6, 2023

Language: Английский

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Nanofibers Loaded Levosalbutamol Sulfate Fast Dissolving Oral Films for Sublingual Delivery

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 99, P. 105920 - 105920

Published: July 3, 2024

Language: Английский

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In vitro investigation the effects of iodinated contrast media on endothelial cell viability, cell cycle, and apoptosis

Toxicology Mechanisms and Methods, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 8

Published: July 31, 2024

In medical practice, iodine contrast media are necessary for diagnostic techniques. However, it comes with a potential risk to the patient in form of allergic reactions. The aim this research is study effects on endothelial cells an vitro system at various concentrations, specifically investigating their impact cell viability, cycle, and apoptosis treated within field radiology. Our results showed that media, when concentration was range 2.5-50 mgI/ml, viability decreased by 50%. Conversely, exposure ioversol concentrations between 12.5 50.0 mgI/ml resulted notable increase percentage total apoptotic cells, including both early late apoptosis. conclusion, our investigation sheds light effect iodinated cycle progression, These findings contribute valuable insights ensure safety use, aligning guidelines radiological procedures. Further adherence established crucial refining understanding promoting safe application

Language: Английский

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Deciphering Steroidal and Aporphine Alkaloids as Antileukemic Agents by Approaches of Molecular Networking and Metabolomics

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The chemodiversity of plants is a valuable resource for drug discovery, and its combination with modern approaches can reduce the time consumption bioactive metabolite discovery. This study aimed to evaluate chemical constituents from 18 plant species different families against leukemia cancer cells application statistical analysis metabolomic data molecular networking prediction metabolites. samples, extracted by an accelerated solvent extractor using ethanol water 7:3 (v/v), were analyzed LC-DAD-MS evaluated (Kasumi-1, KG-1, K-562). Chemical aligned, statistics, applied create network. Sesbania virgata, Aeschynomene denticulata, Erythroxylum angiufugum, Psidium guineense, Astronium fraxinifolium, Coccoloba ochreolata, Solanum glaucophyllum (S. glaucophyllum), Paullinia pinnata inhibited K-562 cell viability approximately 70% at 100 μg/mL, while Ocotea diospyrifolia showed 35% inhibition KG-1 lineage. Alkaloid fractions S. O. revealed EC50 values ranging 13.9 6.4 μg/mL lines, effectively inducing death apoptotic characteristics, membrane integrity loss, signs late apoptosis. was essential crucial complement analysis, which performed 430 features targeted steroidal aporphine alkaloids. Boldine 46, 116, 145 μM Kasumi, respectively. findings marked relevance broader predict compounds, emphasizing potential benefits in search metabolites cells, particularly

Language: Английский

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Data-Driven Quantitative Intrinsic Hazard Criteria for Nanoproduct Development in a Safe-by-Design Paradigm: A Case Study of Silver Nanoforms

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(5), P. 3948 - 3962

Published: Feb. 16, 2023

The current European (EU) policies, that is, the Green Deal, envisage safe and sustainable practices for chemicals, which include nanoforms (NFs), at earliest stages of innovation. A theoretically by design (SSbD) framework has been established from EU collaborative efforts toward definition quantitative criteria in each SSbD dimension, namely, human environmental safety dimension environmental, social, economic sustainability dimensions. In this study, we target demonstrate journey intrinsic hazard derived findable, accessible, interoperable, reusable data. Data were curated merged development new approach methodologies, structure-activity relationship models based on regression classification machine learning algorithms, with intent to predict a class. utilize system (i.e., hydrodynamic size polydispersity index) non-system elemental composition core size)-dependent nanoscale features combination biological vitro attributes experimental conditions various silver NFs, functional antimicrobial textiles, cosmetics applications. second step, interpretable rules (criteria) followed certainty factor obtained exploiting Bayesian network structure crafted expert reasoning. probabilistic model shows predictive capability ≈78% (average accuracy across all classes). work, show how shifted conceptualization realistic implementation pragmatic instances. This study reveals (i) be considered aspects during synthesis stage, (ii) challenges within, (iii) future directions generation distillation such can feed paradigms. Specifically, guide material engineers synthesize NFs are inherently safer alternative nanoformulations, innovation, while enable fast cost-efficient silico toxicological screening previously synthesized hypothetical scenarios yet-to-be NFs.

Language: Английский

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Fabrication and Characterization of Zn Particle Incorporated Fibrous Scaffolds for Potential Application in Tissue Healing and Regeneration

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(42), P. 48913 - 48929

Published: Oct. 17, 2023

Zinc (Zn) metal and its alloys have received a lot of interest in biomedical applications due to their biodegradability, biocompatibility, antimicrobial activity, ability stimulate tissue regeneration. Bulk Zn has been successfully utilized variety implant applications, most notably as bioabsorbable cardiac stents orthopedic fixation devices, where it provides adequate mechanical properties while also releasing helpful ions (Zn2+) during degradation. Such beneficial are dose-dependent and, when released excess, can induce cellular toxicity. In this study, we hypothesize that embedding particles into polymer nanofibrous scaffold will enable control the degradation time release Zn2+. We designed fabricated two scaffolds, polycaprolactone (PCL) polycaprolactone-chitosan (PCL-CH). Each had an increasing amount Zn. Several physicochemical such fiber morphology, crystallinity, strength, hydrophilicity, Zn2+, thermal properties, chemical compositions, so forth were characterized compared with PCL fibrous scaffold. The biological scaffolds evaluated vitro utilizing direct indirect cytotoxicity assays cell viability. All data show addition changed various physical PCL-CH except structure. Further investigation reveals degrade relatively faster than because presence hydrophilic CH influences Zn2+ culture conditions combined advantages enriched 3T3 fibroblast cells' survival proliferation ones higher concentration particles. These new composite promising be further considered for healing regeneration applications.

Language: Английский

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The Influence of Nanoparticles of Graphene Oxide‑PEG on Cytokine Profile of Monocytes from Human Blood In Vitro

Trends in Immunotherapy, Journal Year: 2025, Volume and Issue: 9(1), P. 8 - 22

Published: Jan. 17, 2025

This study investigated the response of human monocytes to co-culture with pegylated (linear or branched) graphene oxide (GO) nanoparticles, specifically examing both small (P-GOs, 100 -200 nm) and larger (P-GOb, 1-5 μm) particles at concentrations 5, 25, 50 µg mL–1. Human (CD14+ cells) were isolated cultured these nanoparticles for 72 hours. We measured cell viability, lactate dehydrogenase (LDH) release, cytokine production. The findings showed that P-GO had little effect on production, including MIF, GM-CSF, VEGF, IP-10, IL-8, HGF, SCGF-beta in vitro. At a low concentration (5 μg mL–1), exhibited minimal influence cytokines, except forthe LP-GOb variant, which increased M-CSF Conversely, 25 mL–1 enhanced release variouscytokines, proinflammatory IL-6, IL-1β, IL-1α, IL-18, IL-17, IL-16, IFN-γ, TNF-β, TNF-α, anti-inflammatory IL-1ra, IL-13, IL-10, IL-4, regulatory G-CSF, IL-2, IL-3, IL-5, IL-12 (p40), (p70), M-CSF, GM-CSF chemokines CTACK, Eotaxin, GRO-α, RANTES, MIP-1β, MCP-1, MIP-1α, MCP-3, MIG, SDF-1α, growth factors Basic FGF, PDGF-BB, SCF, LIF TRAIL. Although higher resulted significant monocyte viability remained largely unaffected . LDH was elevated solely samples treated LP-GOb. BP-GOs profiles, raising levels highest concentration. These results indicate modifying may hold potential creating graphene-based pharmacological agents.

Language: Английский

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Reactive oxygen species responsive dextran-thioketal conjugate nanocarriers for the delivery of hydrophilic payloads

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: 356, P. 123375 - 123375

Published: Feb. 13, 2025

Language: Английский

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Development of Biocompatible Scaffolds of Collagen Fibers from Tilapia Scales (Oreochromis niloticus) Modified with PVA

Fibers and Polymers, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

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