Physiology and Molecular Biology of Plants, Год журнала: 2024, Номер 31(1), С. 41 - 65
Опубликована: Ноя. 22, 2024
Язык: Английский
Plants, Год журнала: 2024, Номер 13(19), С. 2762 - 2762
Опубликована: Окт. 1, 2024
Pesticide use in crops is a severe problem some countries. Each country has its legislation for use, but they differ the degree of tolerance these broadly toxic products. Several synthetic pesticides can cause air, soil, and water pollution, contaminating human food chain other living beings. In addition, them accumulate environment an indeterminate amount time. The agriculture sector must guarantee healthy with sustainable production using environmentally friendly methods. this context, biological biopesticides from microbes plants are growing green solution segment. pests attack worldwide, including weeds, insects, nematodes, microorganisms such as fungi, bacteria, viruses, causing diseases economic losses. bioproducts microorganisms, microbial (MBPs) or alone, practice due to intense research world. Mainly, baculoviruses have been used sources biomolecules secondary metabolites biopesticide use. Different methods, direct soil application, spraying techniques endotherapy, seed treatment, used. Adjuvants like surfactants, protective agents, carriers improve system different formulations. tool bioremediation environment. This review summarizes topics, focusing on origin.
Язык: Английский
Процитировано
7
3 Biotech, Год журнала: 2025, Номер 15(4)
Опубликована: Март 25, 2025
Язык: Английский
Процитировано
0
Frontiers in Plant Science, Год журнала: 2024, Номер 15
Опубликована: Авг. 13, 2024
Accurate and timely diagnosis of plant viral infections plays a key role in effective disease control maintaining agricultural productivity. Recent advances the viruses have significantly expanded our ability to detect monitor pathogens crops. This review discusses latest diagnostic technologies, including both traditional methods innovations. Conventional such as enzyme-linked immunosorbent assay DNA amplification-based assays remain widely used due their reliability accuracy. However, diagnostics next-generation sequencing CRISPR-based detection offer faster, more sensitive specific virus detection. The highlights main advantages limitations systems conventional methods, biosensor technologies advanced sequence-based techniques. In addition, it also effectiveness commercially available tools challenges facing modern techniques well future directions for improving informed management strategies. Understanding features methodologies would enable stakeholders choose optimal strategies against threats ensure global food security.
Язык: Английский
Процитировано
4
Physiology and Molecular Biology of Plants, Год журнала: 2025, Номер unknown
Опубликована: Фев. 17, 2025
Язык: Английский
Процитировано
0
Physiological and Molecular Plant Pathology, Год журнала: 2025, Номер unknown, С. 102703 - 102703
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Horticulturae, Год журнала: 2024, Номер 10(5), С. 501 - 501
Опубликована: Май 13, 2024
A discrete choice experiment was conducted to assess the perceptions and willingness-to-pay of Italian consumers regarding plant diagnosis sustainable cultivation attributes in outdoor ornamental plants, specifically Abelia × grandiflora. The results revealed that most place great importance on health plants during purchasing process, with a preference for obtaining them from nursery facilities. Additionally, they demonstrated willingness pay price premium innovative production A. grandiflora, amounting EUR 1.10 0.90, respectively. These findings have significant implications (i) growers, enabling shape their management marketing strategies, (ii) policymakers, facilitating enhancement communication strategies implementation awareness campaigns aimed at promoting sale healthy species, following current EU regulation 2020/1201.
Язык: Английский
Процитировано
2
Archives of Microbiology, Год журнала: 2024, Номер 206(12)
Опубликована: Ноя. 14, 2024
Язык: Английский
Процитировано
2
Plant Disease, Год журнала: 2024, Номер unknown
Опубликована: Июль 10, 2024
Whiteflies (Bemisia tabaci) are a significant pest of cucurbits and vector many viruses, leading to substantial economic losses. Modern diagnostic tools offer the potential for early detection viruses in whiteflies before crop production. One such tool is multiplex reverse transcriptase quantitative PCR (RT-qPCR) probe-based technique, which can detect multiple targets single reaction simultaneously quantify levels each target, with limit 100 copies per target. In this study, RT-qPCR–based system capable identifying one DNA virus three RNA whiteflies—cucurbit leaf crumple (CuLCrV), cucurbit chlorotic yellows (CCYV), yellow stunting disorder (CYSDV), squash vein yellowing (SqVYV)—was developed. To ensure reliability assay, an internal gene control as fifth target monitor false-negative results was incorporated. This newly developed molecular possesses several advantages. It up five desired from whitefly sample, even at concentrations low 1 ng/μl. evaluate its sensitivity, we conducted experiments using serially diluted cloned plasmids vitro transcribed transcripts viruses. We also assessed specificity assay by including aphid-transmitted other known infect cucurbits. The method successfully detected all allowed quantification mixture healthy RNA. Our aim study develop highly specific sensitive one-step RT-qPCR simultaneous transmitted offers advantages detection, enabling prompt measures mitigate further spread viral infections reduce yield Additionally, demonstrated ability mixed (CCYV, CYSDV, CuLCrV, SqVYV) individual number carried whitefly. outperforms currently available techniques detecting samples given time be effectively utilized monitoring plant symptomless plants.
Язык: Английский
Процитировано
1
ACS Agricultural Science & Technology, Год журнала: 2024, Номер 4(7), С. 759 - 767
Опубликована: Июнь 28, 2024
Язык: Английский
Процитировано
0
Microbiology Spectrum, Год журнала: 2024, Номер 12(9)
Опубликована: Авг. 9, 2024
ABSTRACT Sugarcane yellow leaf virus (SCYLV) can reduce sugarcane productivity. A novel detection system based on reverse transcription-multienzyme isothermal rapid amplification (RT-MIRA) combined with CRISPR-Cas12a, named RT-MIRA-CRISPR-Cas12a, was developed. This innovative approach employs crude extract directly as the reaction template, streamlining extraction process for simplicity and speed. Combining RT-MIRA CRISPR-Cas12a in one tube increases ease of operation while reducing risk aerosol contamination. In addition, it exhibits sensitivity equivalent to qPCR, boasting a lower limit 25 copies. Remarkably, entire process, from sample completion, requires only 52–57 minutes, just thermostat water bath. The result be observed judged by naked eye. IMPORTANCE disease (SCYLD) is an important viral that affects yield. There urgent need rapid, sensitive, stable methods. (RT-MIRA-CRISPR-Cas12a) method established this study has good specificity high sensitivity. showed compatibility stability extract, shown fact positive could still stably detected after 1 week when placed at 4°C. transcription polymerase chain (RT-PCR), transcription-quantitative (RT-qPCR) were used detect SCYLV 33 samples collected field, found three methods reached consistent conclusions. Cas12a-based proves highly suitable on-site SCYLV.
Язык: Английский
Процитировано
0