Genetic resistance among banana somaclones derived from the Grand Naine cultivar (Cavendish, AAA) to Fusarium oxysporum f. sp. cubense, subtropical race 4.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 6, 2025

Banana production is affected by several pests and diseases, of which Fusarium wilt, caused Fusarium oxysporum f. sp. cubense (Foc), notable. Despite the different control measures management used to contain development resistant cultivars has been best approach combating this disease. Therefore, work uses biotechnology aimed induce somaclonal variation using thidiazuron (TDZ) Paclobutrazol® (PBZ) growth regulators banana cultivar ‘Grand Naine’ (Cavendish, AAA), identify variants wilt subtropical race 4 (ST4). Stem apices were cultured in vitro medium MS supplemented with 1 mg L^{− 1} PBZ TDZ. After a series ten subcultures, regenerated plants planted in water tanks containing soil infested Foc greenhouse. At 90 days after inoculation, evaluated for pathogen resistance longitudinal sections near rhizome as well symptoms graded scale. Thirteen somaclones selected Foc. The will be agronomic market potential, validation stability, purpose being recommended farmers future improvement studies.

Язык: Английский

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Indirect regeneration of long-term callus cultures in gladiolus: a protocol for stable genetic fidelity

Frontiers in Horticulture, Год журнала: 2025, Номер 4

Опубликована: Май 21, 2025

Gladiolus is an important and economically valued ornamental plant grown worldwide. One of the major challenges in its micropropagation maintaining genetic stability during indirect regeneration long-term callus maintenance. The objective this study was to develop optimized shoot protocol for three commercial cultivars with consistent traits. Callus initiated from basal part extended mother corm sprout (EMCS) explants MS medium supplemented 2 mgL -1 2,4-D, NAA 1 BAP. synthesis phenolic compounds effectively controlled by addition 150 ascorbic acid, 100 citric 500 activated charcoal. This led 80% decrease accumulation across all comparison control. For regeneration, calli which were maintained over long term transferred BAP, Kin 0.25 NAA. significantly enhanced percentage (95.55%) number (39.44 shoots per explant). Additionally, cormel formation (16.66 cormels explant) at base regenerated plantlets using containing 9% sucrose indole-3-acetic without any Cormels acclimatized greenhouse 100% survival rate. To demonstrate stability, evaluated flow cytometry Inter Simple Sequence Repeat (ISSR) markers, verifying their identification plants. provides a reliable scalable gladiolus, promising applications breeding programs that aim transferring desirable traits such as disease resistance or specific floral features.

Язык: Английский

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Use of Nanomaterials for the in Vitro Production of Plants and Plant Metabolites

Опубликована: Янв. 1, 2025

Язык: Английский

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Producción de plántulas de banano aplicando Bencil Amino Purina en cámaras térmicas

Опубликована: Май 31, 2025

Ecuador is the main exporter of banana in world, so it necessary to look for alternatives maintain yields, which vital importance that seedlings are healthy and vigorous, why objective this research produce by applying doses Benzyl Amino Purine method thermal chambers. The trial was carried out at Cantón La Mana, Ecuador, using a completely randomized design with factorial arrangement, where Factor A BAP (0,10,20,30 40 mg L-1) B varieties (Gros Michael, Williams Valery). variables evaluated were number length roots: fresh weight aerial root biomass. results study coincide those expressed different authors about advantages application BPA In conclusion, had significant effect on growth variables, differential behavior depending cultivar evaluated, will determine dose be applied obtain optimum yields

Язык: Английский

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An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Янв. 11, 2024

Abstract Background The development of the plant in vitro techniques has brought about variation identified regenerants known as somaclonal or tissue culture-induced (TCIV). S-adenosyl-L-methionine (SAM), glutathione (GSH), low methylated pectins (LMP), and Cu(II) ions may be implicated green regeneration efficiency (GPRE) TCIV, according to studies barley ( Hordeum vulgare L.) partially triticale (× Triticosecale spp. Wittmack ex A. Camus 1927). Using structural equation models (SEM), these metabolites have been connected metabolic pathways (Krebs Yang cycles, glycolysis, transsulfuration), but not for triticale. metabolomic (epi)genetic data, study sought develop a statistical model. culture’s induction medium was supplemented with various quantities Ag(I) regeneration. period also changed. donor plant, anther-derived regenerants, metAFLP were utilized analyze TCIV concerning DNA symmetric (CG, CHG) asymmetric (CHH) sequence contexts. Attenuated Total Reflectance–Fourier Transfer Infrared (ATR-FTIR) spectroscopy used gather information on LMP, SAM, GSH. To frame model employed. Results According analysis, average change CHH context 8.65%, 0.58% de novo methylation. Absorbances FTIR spectra regions specific GSH variables values introduced SEM number per 100 plated anthers 2.55. Conclusions amounts pectin demethylation, methylation, are explain GPRE. By altering concentration medium, which influences amount pectin, triticale’s GPRE can increased.

Язык: Английский

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Exploring indirect somatic embryogenesis and somaclonal variation for propagation of three Coffea arabica L. cultivars

Chilean journal of agricultural research, Год журнала: 2024, Номер 84(1), С. 15 - 27

Опубликована: Янв. 26, 2024

The propagation of arabica coffee (Coffea L.) seedlings through indirect primary embryogenesis has significant potential to produce many healthy relatively quickly. However, there is a concern that plant growth regulators (PGRs) applied in the culture media could result somaclonal variations. To address this issue, appropriate concentration PGR needs be identified increase success somatic process while preventing This study aimed develop protocol for C. and evaluate variation plantlets derived from embryogenesis, considering genotype used. Three cultivars coffee, i.e., AS2K, S-795, Sigarar Utang, two types PGRs (2,4-D 2-iP) at different combination concentrations, were used as factorial experimental design. was 4.52 9.04 µM 2,4 D 4.93, 9.86, 14.79, 19.72, 24.65 2-iP. All combinations replicated 10 times. findings revealed influenced percentage callus formation which ranged 87.02% 93.81%, significantly impacted development torpedoes germination embryos with number germinated 32.20-94.20 21.10-82.20 ‘AS2K’, 37.90-62.90 27.70-50.20 ‘S-795’, 39.40-72.00 29.60-60.60 ‘Sigarar Utang’, respectively. Among evaluated, 2,4-D 19.72 2-iP demonstrated highest efficacy 94.20 82.20 whereas 14.79 preferred ‘S-795’ Utang’. Furthermore, molecular analysis using SSR primers resulting indicated no evidence variation.

Язык: Английский

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Exploring the Significance of Somaclonal Variations in Horticultural Crops

Molecular Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Июнь 19, 2024

Язык: Английский

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Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production

Molecules, Год журнала: 2023, Номер 28(12), С. 4880 - 4880

Опубликована: Июнь 20, 2023

The in vitro cultures of Rindera graeca, a rare endemic plant, were developed as sustainable source phenolic acids. Various shoot and root established scaled up sprinkle bioreactor. A multiplication rate 7.2 shoots per explant was achieved. HPLC–PDA–ESI–HRMS analysis revealed the presence rosmarinic acid (RA) lithospermic B (LAB) main secondary metabolites both cultures. maximum RA (30.0 ± 3.2 mg/g DW) LAB (49.3 ±15.5 yields determined root-regenerated shoots. strongest free radical scavenging activity (87.4 1.1%), according to 2,2-diphenyl-1-picrylhydrazyl-hydrate assay, noted for roots cultivated DCR medium. highest reducing power (2.3 µM 0.4 TE/g DW), by ferric-reducing antioxidant on an SH medium containing 0.5 mg/L 6-benzylaminopurine. genetic performed using random amplified polymorphic DNA start codon targeted markers variation 62.8% 96.5% among investigated roots. This variability reflects capacity produce compounds.

Язык: Английский

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Selection and Characterization of Somaclonal Variants of Prata Banana (AAB) Resistant to Fusarium Wilt

Agronomy, Год журнала: 2024, Номер 14(8), С. 1740 - 1740

Опубликована: Авг. 8, 2024

Fusarium wilt, caused by the fungus oxysporum f. sp. cubense (Foc), is one of most devastating diseases affecting banana cultivation worldwide. Although Foc tropical race 4 (TR4) has not yet been identified in Brazilian production areas, damage races 1 and subtropical main cause losses, especially cultivars Prata subgroup. Thus, induction somaclonal variation a promising strategy biotechnology to generate genetic variability develop resistant varieties. This study aimed induce Catarina cultivar (AAB genome) using successive subcultures Murashige Skoog (MS) medium enriched with plant regulator Thiadizuron (TDZ) at two concentrations: 2 mg/L. After evaluating symptoms, we selected 13 somaclones that were infected fungus. Histochemical histological analyses indicated possible defense mechanisms prevented colonization and/or infection Foc, such as intense phenolic compounds presence cellulose callose roots. Some showed no pathogen structures xylem-conducting vessels, indicating pre-penetration resistance. Furthermore, molecular studies alterations may have induced resistance without compromising agronomic characteristics commercial genotype.

Язык: Английский

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Molecular Marker Applications in the Selection of Elite Genotypes for Plant Stress Tolerance and Genetic Fidelity

OBM Genetics, Год журнала: 2024, Номер 08(03), С. 1 - 25

Опубликована: Сен. 2, 2024

Molecular markers play a crucial role in accelerating crop production for sustainable agriculture by identifying resistant traits and enhancing genetic diversity. In this review, we examine the impact of molecular on advancing our understanding stress tolerance mechanisms plants, addressing pressing imperative to bolster global food meet escalating demands growing population. Additionally, application evaluate accuracy plants due changes caused somaclonal variation during <em>in vitro</em> propagation is mentioned. Specifically, highlight emerging technologies like MAS, MARS, MABC, GWAS, GS, DH production, speed breeding, genome editing, which offer promising opportunities enhance integrity cultivars, aiding security challenges. The literature search focused studies published last ten years. We utilized combination specific broad keywords such as stability, propagation, markers, abiotic-biotic stress, plant biotechnology. conclusion, review analyzes use assessing fidelity grown plants.

Язык: Английский

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