An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 11, 2024

Abstract Background The development of the plant in vitro techniques has brought about variation identified regenerants known as somaclonal or tissue culture-induced (TCIV). S-adenosyl-L-methionine (SAM), glutathione (GSH), low methylated pectins (LMP), and Cu(II) ions may be implicated green regeneration efficiency (GPRE) TCIV, according to studies barley ( Hordeum vulgare L.) partially triticale (× Triticosecale spp. Wittmack ex A. Camus 1927). Using structural equation models (SEM), these metabolites have been connected metabolic pathways (Krebs Yang cycles, glycolysis, transsulfuration), but not for triticale. metabolomic (epi)genetic data, study sought develop a statistical model. culture’s induction medium was supplemented with various quantities Ag(I) regeneration. period also changed. donor plant, anther-derived regenerants, metAFLP were utilized analyze TCIV concerning DNA symmetric (CG, CHG) asymmetric (CHH) sequence contexts. Attenuated Total Reflectance–Fourier Transfer Infrared (ATR-FTIR) spectroscopy used gather information on LMP, SAM, GSH. To frame model employed. Results According analysis, average change CHH context 8.65%, 0.58% de novo methylation. Absorbances FTIR spectra regions specific GSH variables values introduced SEM number per 100 plated anthers 2.55. Conclusions amounts pectin demethylation, methylation, are explain GPRE. By altering concentration medium, which influences amount pectin, triticale’s GPRE can increased.

Language: Английский

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Exploring indirect somatic embryogenesis and somaclonal variation for propagation of three Coffea arabica L. cultivars

Chilean journal of agricultural research, Journal Year: 2024, Volume and Issue: 84(1), P. 15 - 27

Published: Jan. 26, 2024

The propagation of arabica coffee (Coffea L.) seedlings through indirect primary embryogenesis has significant potential to produce many healthy relatively quickly. However, there is a concern that plant growth regulators (PGRs) applied in the culture media could result somaclonal variations. To address this issue, appropriate concentration PGR needs be identified increase success somatic process while preventing This study aimed develop protocol for C. and evaluate variation plantlets derived from embryogenesis, considering genotype used. Three cultivars coffee, i.e., AS2K, S-795, Sigarar Utang, two types PGRs (2,4-D 2-iP) at different combination concentrations, were used as factorial experimental design. was 4.52 9.04 µM 2,4 D 4.93, 9.86, 14.79, 19.72, 24.65 2-iP. All combinations replicated 10 times. findings revealed influenced percentage callus formation which ranged 87.02% 93.81%, significantly impacted development torpedoes germination embryos with number germinated 32.20-94.20 21.10-82.20 ‘AS2K’, 37.90-62.90 27.70-50.20 ‘S-795’, 39.40-72.00 29.60-60.60 ‘Sigarar Utang’, respectively. Among evaluated, 2,4-D 19.72 2-iP demonstrated highest efficacy 94.20 82.20 whereas 14.79 preferred ‘S-795’ Utang’. Furthermore, molecular analysis using SSR primers resulting indicated no evidence variation.

Language: Английский

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Biotechnological Advancements Toward Sugarcane Crop Improvement

Published: Jan. 1, 2024

Sugarcane, is a vital tropical C4 plant known for its sucrose storage capacity, plays crucial role in global sugar and ethanol production. Despite multifaceted applications, the sugarcane industry faces numerous challenges, which impact final harvesting yield quality. Traditional breeding methods prove inefficient due to sugarcane's complexity extended cycles. Modern biotechnological approaches, particularly genetic engineering molecular markers, offer unprecedented opportunities. These techniques enable precise modifications, facilitating development of superior varieties. Molecular markers genomic tools aid identifying high-yield, stress-tolerant cultivars, enhancing selection processes. Additionally, advancements bioinformatics, genomics, proteomics, transcripteomic shed light on intricate gene-protein-metabolite relationships, understanding stress responses. This chapter explores these innovations, showcasing their potential address existing challenges ensure sustainable future production, thereby promoting food security sustainability.

Language: Английский

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Exploring the Significance of Somaclonal Variations in Horticultural Crops

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Language: Английский

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Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production

Molecules, Journal Year: 2023, Volume and Issue: 28(12), P. 4880 - 4880

Published: June 20, 2023

The in vitro cultures of Rindera graeca, a rare endemic plant, were developed as sustainable source phenolic acids. Various shoot and root established scaled up sprinkle bioreactor. A multiplication rate 7.2 shoots per explant was achieved. HPLC–PDA–ESI–HRMS analysis revealed the presence rosmarinic acid (RA) lithospermic B (LAB) main secondary metabolites both cultures. maximum RA (30.0 ± 3.2 mg/g DW) LAB (49.3 ±15.5 yields determined root-regenerated shoots. strongest free radical scavenging activity (87.4 1.1%), according to 2,2-diphenyl-1-picrylhydrazyl-hydrate assay, noted for roots cultivated DCR medium. highest reducing power (2.3 µM 0.4 TE/g DW), by ferric-reducing antioxidant on an SH medium containing 0.5 mg/L 6-benzylaminopurine. genetic performed using random amplified polymorphic DNA start codon targeted markers variation 62.8% 96.5% among investigated roots. This variability reflects capacity produce compounds.

Language: Английский

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Molecular Marker Applications in the Selection of Elite Genotypes for Plant Stress Tolerance and Genetic Fidelity

OBM Genetics, Journal Year: 2024, Volume and Issue: 08(03), P. 1 - 25

Published: Sept. 2, 2024

Molecular markers play a crucial role in accelerating crop production for sustainable agriculture by identifying resistant traits and enhancing genetic diversity. In this review, we examine the impact of molecular on advancing our understanding stress tolerance mechanisms plants, addressing pressing imperative to bolster global food meet escalating demands growing population. Additionally, application evaluate accuracy plants due changes caused somaclonal variation during <em>in vitro</em> propagation is mentioned. Specifically, highlight emerging technologies like MAS, MARS, MABC, GWAS, GS, DH production, speed breeding, genome editing, which offer promising opportunities enhance integrity cultivars, aiding security challenges. The literature search focused studies published last ten years. We utilized combination specific broad keywords such as stability, propagation, markers, abiotic-biotic stress, plant biotechnology. conclusion, review analyzes use assessing fidelity grown plants.

Language: Английский

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Selection of Superior Potato (Solanum tuberosum L.) by Combined Stability Analysis for Future Breeding Strategies

Agriculture (Pol nohospodárstvo), Journal Year: 2024, Volume and Issue: 70(2), P. 72 - 86

Published: July 1, 2024

Abstract Potato is one of the main agricultural commodities and has high economic value. Yield a trait that becomes benchmark for user (industry farmers) in selecting developing varieties. The selection superior newly potato somaclones using combined analysis sustainability index (SI) still underreported. This study aimed to identify effect genotype by environment (growing season) interactions (GEIs), as well select somaclones. research was conducted three years (2020 ‒ 2022) Lembang, West Java, Indonesia. There were 38 tested field, consisting 37 gamma ray-derived control genotype. originated from commercial varieties Agria, Granola, Repita, Vega. field trial used randomized block design repeated times each year. GEIs calculated based on ANOVA. stability assessed (parametric non-parametric), plus interaction (GGE) biplot, (SI). (G), seasons (E) found have highly significant influence yield ( P < 0.01). According ANOVA, impact accounted 37.38% yield’s total sum squares. Combining parametric non-parametric measurements, seven somaclones, P3, P4, P5, P6, P8, P9, P26, selected. GGE biplot selected five stable namely P22, P23, while SI eight high-yielding P13, P16, P32. Based various genotypes been identified P4 P26. can assist activities determine

Language: Английский

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Development of efficient embryo-derived regeneration system and optimization of genetic transformation in cumin (Cuminum cyminum L.)

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2023, Volume and Issue: 155(1), P. 297 - 312

Published: Sept. 9, 2023

Language: Английский

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Development of a sequence-characterized amplified region (SCAR) marker for female off-season flowering detection in date palm (Phoenix dactylifera L.)

Journal of Plant Biotechnology, Journal Year: 2023, Volume and Issue: 50

Published: Oct. 31, 2023

palm (Phoenix dactylifera L.: Arecaceae) is a dioecious species where only female trees bear fruits.In their natural state, date palms produce dates once year.However, in Thailand, some were observed to during the off-season, despite no variations morphology.The availability of such off-season fruits can significantly increase market value.Interestingly, most investigated this study obtained through micropropagation.Hence, there an urgent need for genetic markers distinguish offseason flowering plantlets within tissue culture systems.In study, we aimed develop random amplification polymorphic DNA-sequence characterized amplified region (RAPD-SCAR) identification cultivated Thailand.A total 160 decamer primers employed screen specific RAPD male and populations.Out these, one primer, OPN-02, generated distinct genomic DNA patterns (FOFdp) individuals compared seasonal genotypes.Based on RAPD-specific sequence, SCAR denoted as FOFdpF FOFdpR developed.These single 517-bp fragment, predominantly found populations, with accuracy rate 60%.These findings underscore potential marker technology tracking palms.Notably, BLAST analysis revealed substantial similarity between sequence transcript variant mRNA from Phoenix encoding SET DOMAIN GROUP 40 protein.In Arabidopsis, protein involved epigenetic regulation time.The traits warrants further elucidation.

Language: Английский

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A simple and efficient Agrobacterium tumefaciens mediated transgenic system for tetraploid potato cultivar Desiree

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 7, 2024

Abstract As the fourth staple food crop in world, potatoes can provide enough energy, protein, and nutrients necessary for humans. However, population growth negative effects of climate change call improved potato yields resilience. Genetic engineering is a convenient way to improve varieties create new germplasm resources. Agrobacterium tumefaciens mediated transformation stable widely used method genetic improvement. In this study, simple efficient transgenic system was built by testing different materials, explants type, selection medium, agent, A. EHA105 strain carried vectors. The results shown produced positive transformed seedlings about 40 days. lower ploidy ratio tetraploid variety Desiree compared diploid recipient. Both SEG SE could function as single regeneration medium. Leaf discs eligible. concentration 20μg/L suitable chlorsulfuron selection. efficiency recipient up 68.79%, This fast provides powerful tool supporting basic research on functional genes creating materials breeding.

Language: Английский

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