Antimicrobial Peptides and Cell-Penetrating Peptides: Non-Antibiotic Membrane-Targeting Strategies Against Bacterial Infections

Infection and Drug Resistance, Год журнала: 2023, Номер Volume 16, С. 1203 - 1219

Опубликована: Фев. 1, 2023

The prevalence of antimicrobial resistance (AMR) has been rising quickly in recent years. AMR emerged as a significant obstacle to the treatment infectious diseases, and many attempts have made over past decades find best antimicrobials overcome it. Therefore, it is crucial new medicines combat global rise AMR. Antimicrobial peptides (AMPs) cell-penetrating (CPPs), which target membranes, are promising antibiotic substitutes. AMPs CPPs short amino acid sequences with antibacterial activity well possible therapeutic benefits. In this review, we provide thorough systematic introduction advancement research on CPPs, including information their classification, mechanism action, current state application, limitations optimization.

Язык: Английский

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Antimicrobial Peptides, Their Production, and Potential in the Fight Against Antibiotic-Resistant Pathogens

Fermentation, Год журнала: 2025, Номер 11(1), С. 36 - 36

Опубликована: Янв. 17, 2025

The article reviews the literature on antimicrobial peptides (AMPs) that exhibit unique mechanisms, such as broad-spectrum activity, low development of resistance, and ability to modulate immune response host organism. Information is provided significant potential AMPs in fight against pathogens threatening human health food safety. Enrichment diet with biologically active obtained using proteolytic activity lactic acid bacteria (LAB) proposed a simple, accessible, viable alternative antibiotics does not have harmful side effect. review briefly covers methods for obtaining features LAB system responsible producing bioactive environment. It has been shown various strains makes it possible produce high-quality whey-based beverages different directions antagonistic opportunistic helps optimize gastrointestinal microbiota. assumed drinks can reduce dose antimicrobials combined therapy infectious diseases be preventive measure contagion spread resistance.

Язык: Английский

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Targeting nucleic acid phase transitions as a mechanism of action for antimicrobial peptides

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 7, 2023

Abstract Antimicrobial peptides (AMPs), which combat bacterial infections by disrupting the cell membrane or interacting with intracellular targets, are naturally produced a number of different organisms, and increasingly also explored as therapeutics. However, mechanisms AMPs act on targets not well understood. Using machine learning-based sequence analysis, we identified significant that have strong tendency to form liquid-like condensates in presence nucleic acids through phase separation. We demonstrate this separation propensity is linked effectiveness inhibiting transcription translation vitro, their ability compact clusters cells. These results suggest AMP-driven compaction modulation transitions constitute previously unrecognised mechanism exert antibacterial effects. The development antimicrobials target acid may become an attractive route finding effective long-lasting antibiotics.

Язык: Английский

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No country for old antibiotics! Antimicrobial peptides (AMPs) as next-generation treatment for skin and soft tissue infection

International Journal of Pharmaceutics, Год журнала: 2023, Номер 642, С. 123169 - 123169

Опубликована: Июнь 24, 2023

Язык: Английский

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Design strategies for positively charged endolysins: Insights into Artilysin development

Biotechnology Advances, Год журнала: 2023, Номер 69, С. 108250 - 108250

Опубликована: Сен. 6, 2023

Endolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for development novel antibacterial agents. The use genetic engineering techniques production and modification endolysins offers opportunity to customize their properties activity against specific targets, paving way personalized therapies infections. Gram-negative bacteria possess outer membrane hinder action recombinantly produced endolysins. However, certain capable crossing by virtue segments share resembling those cationic peptides. These regions increase affinity endolysin towards surface assist in permeabilization membrane. In order improve bactericidal effectiveness endolysins, approaches have been implemented net charge, including Artilysins containing positively charged amino acids at one end. At present, there no guidelines outlining steps implementing these modifications. There is ongoing debate surrounding optimal location positive need a linker region, acid composition peptides modifying aim this study provide clarity on topics analyzing comparing most effective modifications found previous literature.

Язык: Английский

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Dual Antibiotic Approach: Synthesis and Antibacterial Activity of Antibiotic–Antimicrobial Peptide Conjugates

Antibiotics, Год журнала: 2024, Номер 13(8), С. 783 - 783

Опубликована: Авг. 21, 2024

In recent years, bacterial resistance to conventional antibiotics has become a major concern in the medical field. The global misuse of clinics, personal use, and agriculture accelerated this resistance, making infections increasingly difficult treat rendering new ineffective more quickly. Finding is challenging due complexity mechanisms, high costs low financial incentives for development molecular scaffolds, stringent regulatory requirements. Additionally, innovation slowed, with many being modifications existing drugs rather than entirely classes. Antimicrobial peptides (AMPs) are valid alternative small-molecule offering several advantages, including broad-spectrum activity lower likelihood inducing their multifaceted mechanisms action. However, AMPs face challenges such as stability issues physiological conditions, potential toxicity human cells, production costs, difficulties large-scale manufacturing. A reliable strategy overcome drawbacks associated use combination therapy, namely simultaneous co-administration two or synthesis covalently linked conjugates. This review aims provide comprehensive overview literature on antibiotic-AMP conjugates, particular emphasis critically analyzing design synthetic strategies employed creation. addition synthesis, will also explore reported antibacterial these conjugates and, where available, examine any data concerning cytotoxicity.

Язык: Английский

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Tuning the Lipophilicity of New Ciprofloxacin Derivatives in Selected Eskape Bacteria with Emphasis on E. Coli Mutants

Опубликована: Янв. 1, 2025

Язык: Английский

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Tuning the lipophilicity of new ciprofloxacin derivatives in selected ESKAPE bacteria with emphasis on E. coli mutants

Bioorganic Chemistry, Год журнала: 2025, Номер 158, С. 108324 - 108324

Опубликована: Март 4, 2025

Язык: Английский

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RNA toehold switch-based reporter assay to assess bacterial uptake of antisense oligomers

mBio, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

ABSTRACT Antisense oligomers (ASOs) hold promise as antibiotics for the selective targeting of bacterial pathogens and tools modulation gene expression in microbes that are not amenable to genetic engineering. However, their efficient delivery across complex envelope remains a major challenge. There few methods assess efficiency carrier-mediated ASO uptake by bacteria. Here, we have developed “switch-on” reporter assay measure semi-quantitative manner. The uses synthetic RNA toehold switch fused mRNA fluorescent protein, which is activated vivo peptide nucleic acid (PNA)-based upon into cytosol. We used this screen different cell-penetrating peptides (CPPs) carriers Escherichia coli Salmonella enterica observed up 60-fold activation, depending on CPP strain used. Our shows high dynamic range sensitivity, should enable high-throughput screens carriers. also show can be study routes PNA uptake, demonstrated reduced activity absence inner membrane protein SbmA. In summary, present tool discovery species-specific will useful broader investigation cellular mechanisms antibacterial ASOs. IMPORTANCE rise antimicrobial resistance presents global health If addressed, death toll from resistant infections expected dramatically coming years. As result, it essential explore alternative therapies. One promising approach target mRNAs using antisense silence genes involved functions, virulence, or resistance. delivering ASOs membranes challenge effective monitor limited. study, develop facilitate This research paves way developing novel precision antisense-based

Язык: Английский

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Cell-Penetrating Antimicrobial Peptides with Anti-Infective Activity against Intracellular Pathogens

Antibiotics, Год журнала: 2022, Номер 11(12), С. 1772 - 1772

Опубликована: Дек. 8, 2022

Cell-penetrating peptides (CPPs) are natural or engineered peptide sequences with the intrinsic ability to internalize into a diversity of cell types and simultaneously transport hydrophilic molecules nanomaterials, which cellular uptake is often limited. In addition this primordial activity penetration without membrane disruption, multivalent antimicrobial accompanies some CPPs. Antimicrobial (AMPs) cell-penetrability exert their effect intracellularly, they great interest. CPPs (CPAPs) comprise particular class bioactive that arise as promising agents against difficult-to-treat intracellular infections. This short review aims present antibacterial, antiparasitic, antiviral effects various cell-penetrating currently documented. Examples include different CPAPs bacteria can propagate like

Язык: Английский

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