Modulation of GPER1 alleviates early brain injury via inhibition of A1 reactive astrocytes activation after intracerebral hemorrhage in mice

Heliyon, Год журнала: 2024, Номер 10(5), С. e26909 - e26909

Опубликована: Фев. 25, 2024

BackgroundEarly brain injury (EBI) caused by inflammatory responses in acute phase of Intracerebral hemorrhage (ICH) plays a vital role the pathological progression ICH. Increasing evidences demonstrate A1 reactive astrocytes are associated with severity EBI. G-protein coupled estrogen receptor 1 (GPER1) has been proved mediating neuroprotective effects central nervous system (CNS) disease. However, whether GPER1 protective effect on ICH and activation is not well studied.MethodsICH model was established infused autologous whole blood into right basal ganglia wild type knockout mice. specific agonist G1 antagonist G15 were administered intraperitoneal injection at h or 0.5 after Neurological function detected day 3 open field test corner turn following Besides, determined immunofluorescence staining 3. To further identify possible mechanism mediated effect, Western blot assays performed 3.ResultsAfter ICH, treatment alleviated mice neurobehavior deficits Meanwhile, also significantly reduced GFAP positive C3 cells Interestingly, reversed expression GFAP+C3+ GPER1. Mechanistic studies indicated TLR4 NF-κB GPER1.ConclusionGenerally, EBI through inhibiting via TLR4/NF-κB pathway Additionally, GPER1may be promising target for treatment.

Язык: Английский

---

A Focus on Astrocyte Contribution to Parkinson’s Disease Etiology

Biomolecules, Год журнала: 2022, Номер 12(12), С. 1745 - 1745

Опубликована: Ноя. 24, 2022

Parkinson’s disease (PD) is an incurable neurodegenerative of high prevalence, characterized by the prominent death dopaminergic neurons in substantia nigra pars compacta, which produces dopamine deficiency, leading to classic motor symptoms. Although PD has traditionally been considered as a neuronal cell autonomous pathology, damage vulnerable responsible for disease, growing evidence strongly suggests that astrocytes might have active role neurodegeneration observed. In present review, we discuss several studies evidencing astrocyte implications PD, highlighting consequences both loss normal homeostatic functions and gain toxic wellbeing neurons. The revised information provides significant allows be positioned crucial players etiology, factor needs taken into account when considering therapeutic targets treatment disease.

Язык: Английский

---

Octadecaneuropeptide, ODN, Promotes Cell Survival against 6-OHDA-Induced Oxidative Stress and Apoptosis by Modulating the Expression of miR-34b, miR-29a, and miR-21in Cultured Astrocytes

Cells, Год журнала: 2024, Номер 13(14), С. 1188 - 1188

Опубликована: Июль 12, 2024

Astrocytes specifically synthesize and release endozepines, a family of regulatory peptides including octadecaneuropeptide (ODN). We have previously reported that ODN rescues neurons astrocytes from 6-OHDA-induced oxidative stress cell death. The purpose this study was to examine the potential implication miR-34b, miR-29a, miR-21 in protective activity on death cultured rat astrocytes. Flow cytometry analysis showed 6-OHDA increased number early apoptotic dead cells while treatment with subnanomolar dose significantly reduced induced by 6-OHDA. 6-OHDA-treated exhibited over-expression (+118%) associated knockdown miR-34b (−61%) miR-29a (−49%). Co-treatment blocked 6-OHDA-stimulated production ROS NO stimulation Bax caspase-3 gene transcription. Concomitantly, down-regulated expression rescued 6-OHDA-associated miR21, indicating regulates their during Transfection miR-21-3p inhibitor prevented effect against In conclusion, our indicated (i) miRNAs is modified under injury (ii) prevents deregulation induce its neuroprotective action. present identified as an emerging candidate promising pharmacological target opens new therapeutic strategies neurodegenerative diseases, especially Parkinson’s disease.

Язык: Английский

---

Active ingredients of Chinese medicine with immunomodulatory properties: NF-κB pathway and Parkinson's disease

Brain Research, Год журнала: 2023, Номер 1822, С. 148603 - 148603

Опубликована: Сен. 23, 2023

Язык: Английский

---

Vitamin D3 actions on astrocyte cells: A target for therapeutic strategy in Parkinson’s disease?

Neuroscience Letters, Год журнала: 2022, Номер 793, С. 136997 - 136997

Опубликована: Дек. 2, 2022

Язык: Английский

---