Ratiometric electrochemical biosensor based on Cu(II) modified covalent organic framework for the ultra-sensitive and specific detection of glutathione

Chemical Engineering Journal, Год журнала: 2024, Номер 492, С. 152271 - 152271

Опубликована: Май 15, 2024

Язык: Английский

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Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum

Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 11

Опубликована: Янв. 7, 2025

Chronic inflammation caused by Escherichia coli infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays central role not only in regulating ovary cycle, but also implantation of embryo maintenance early pregnancy through secretion progesterone. Understanding intricate interplay between inflammatory processes organ's function is crucial for development effective therapeutic strategies to alleviate disorders improve aim this study was determine vitro effects lipopolysaccharide (LPS) proteomic profile porcine CL mid-luteal phase estrous cycle using LC-MS/MS analysis. slices were incubated presence LPS 24 h. We identified 12 differentially regulated proteins after treatment with (7 them upregulated, while 5 downregulated). analysis showed that these are involved such as glucose metabolism, tricarboxylic acid (TCA), detoxification well steroid biosynthesis CL. Moreover, we demonstrated decreases levels increases progesterone These findings suggest modulates key metabolic pathways CL, potentially impacting its functional activity.

Язык: Английский

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Establishing reference intervals for serum glutathione reductase in healthy adults

Archives of Medical Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Introduction Glutathione reductase (GR) is a key enzyme that catalyzes the reduction of oxidized glutathione to its reduced form, playing vital role in protecting cells from oxidative damage caused by accumulation reactive oxygen species. This study employed ultraviolet enzymatic method measure serum GR activity, aiming investigate distribution among apparently healthy adults and preliminarily establish reference intervals for GR. Material methods A total 6,180 individuals participated this study. Serum activity was measured using AU5800 fully automated biochemical analyzer corresponding commercial kit. The Kolmogorov-Smirnov test used analyze activity. Following guidelines C28-A3 WS/T 402-2024, interval determined bilateral percentile method, covering range 2.5th 97.5th percentile. Results exhibited non-normal distribution. Significant gender-based differences were observed, while no significant variations found across different age groups. Based on these findings, gender-specific established: 26.6–51.8 U/l males 29.7–55.3 females. Conclusions first adults. These provide essential guidance health screening improve diagnosis management related clinical conditions.

Язык: Английский

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A novel fluorescent sensing platform for glutathione based on Förster resonance energy transfer and aggregation-induced emission

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер unknown, С. 126131 - 126131

Опубликована: Март 1, 2025

Язык: Английский

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Enhancing glutathione delivery and efficacy: a novel microcapsule approach

Journal of Materials Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Язык: Английский

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Genome-Based in silico Evaluation of the Biosafety and Probiotic Traits of Limosilactobacillus fermentum J23 Isolated from Artisanal Mexican Cheese

Food Biotechnology, Год журнала: 2024, Номер 38(1), С. 1 - 29

Опубликована: Янв. 2, 2024

Comprehensive in silico analysis aimed to identify the genes involved biosafety and probiotic features of Limosilactobacillus fermentum J23 was performed. The strain had a single circular chromosome 2,004,882 base pairs (bp), with Guanine + Cytosine (G C) content 51.74%. contained 2,001 predicted as protein-coding sequences (CDS), 86 pseudogenes, 56 tRNAs, five rRNAs, three ncRNAs. demonstrated absence risk-related corresponding transferable/acquirable antibiotic resistance, virulence factors, toxic metabolites, detrimental metabolic/enzymatic activities. nonpathogenic humans presence variety potentially related its profile were revealed, including those carbohydrate metabolism transport, adhesion aggregation ability, stress/adaptive response, CRISPR/Cas adaptive immunity, immunomodulation. These findings highlight potential L. provide genomic clues advance future applications.

Язык: Английский

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Poly(Vinyl Alcohol)/Bovine Serum Albumin Hybrid Hydrogels with Tunable Mechanical Properties

Polymers, Год журнала: 2023, Номер 15(23), С. 4611 - 4611

Опубликована: Дек. 4, 2023

In this study, a new strategy was adopted for obtaining polymer/protein hybrid hydrogels with shape stability and tunable mechanical or rheological characteristics by using non-toxic procedures. A chemical network created poly(vinyl alcohol)(PVA)/bovine serum albumin (BSA) mixture in aqueous solution the presence of genipin reduced glutathione (GSH). Then, second physical formed through PVA after applying freezing/thawing cycles. addition, protein macromolecules intermolecular disulfide bridges GSH. these conditions, multiple crosslinked networks were obtained, determining strengthening stiffening into relatively tough porous viscoelasticity self-healing ability. SEM analysis evidenced formation interconnected pores sizes between 20 μm 50 μm. The investigations showed that hydrogels’ strength response different conditions deformation influenced composition crosslinking procedure. Thus, dynamics can be adjusted to mimic viscoelastic properties native tissues. dynamic water vapor-sorption ability, swelling behavior an environment, bioadhesive also investigated are discussed paper. designed on request, they promising candidates tissue engineering, bioinks, wound dressing applications.

Язык: Английский

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Network-based Multi-omics Disease–Drug Associations Reveal Drug Repurposing Candidates for Covid-19 Disease Phases

Drug repurposing, Год журнала: 2024, Номер 1(1)

Опубликована: Июль 3, 2024

Background The development and rollout of vaccines the use various drugs have contributed to controlling coronavirus disease 2019 (Covid-19) pandemic. Nevertheless, challenges such as inequitable distribution vaccines, influence emerging viral lineages immunoevasive variants on vaccine efficacy, inadequate immune defense in subgroups population continue motivate new combat disease. Aim In this study, we sought identify, prioritize, characterize drug repurposing candidates appropriate for treating mild, moderate, or severe Covid-19 using a network-based integrative approach that systematically integrates drug-related data multi-omics datasets. Methods We leveraged used random walk with restart algorithm explore an integrated knowledge graph comprising three subgraphs: (i) graph, (ii) (iii) state-specific omics graph. Results prioritized 20 US Food Drug Administration-approved agents potential candidate phases. Specifically, could stimulate cell recruitment activation including histamine, curcumin, paclitaxel utility mild states mitigate progression. Drugs like omacetaxine, crizotinib, vorinostat exhibit antiviral properties inhibit replication can be considered moderate states. Also, given association between antioxidant deficiency high inflammatory factors trigger cytokine storms, antioxidants glutathione potent anti-inflammatory effects (sarilumab tocilizumab), corticosteroids (dexamethasone hydrocortisone), immunosuppressives (sirolimus cyclosporine) are hyperinflammatory cascade Covid-19. Conclusion Our study demonstrates data-driven analysis within enables prioritizing phases, offering comprehensive basis therapeutic strategies brought market quickly their established safety profiles. Importantly, implemented here prioritize other diseases.

Язык: Английский

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Horseradish peroxidase/graphene electrochemical biosensor for glutathione detection

Nanomaterials and Energy, Год журнала: 2024, Номер 13(3), С. 102 - 109

Опубликована: Авг. 6, 2024

Glutathione (GSH) is a tripeptide composed of three amino acids: cysteine, glycine, and glutamate. It essential to many different living things as an antioxidant. GSH serves key biomarker its detection crucial for studying levels in bodies, providing insights into various diseases. In this work, we present the use highly sensitive electrochemical sensor detect utilizing graphene oxide (GO)–modified indium tin (ITO) electrode immobilized with enzyme horseradish peroxidase (HRP). The modified was further characterized by Fourier transform infrared spectroscopy, spectrum showed that HRP successfully onto GO. behavior ITO examined cyclic voltammetry. Cyclic voltammetry demonstrated HRP/GO/ITO has better electrocatalytic activity than bare oxidation acetate buffer solution. had 1 µM limit linear range 10 50 µM. resulted improved properties, long-term stability, good repeatability, high sensitivity, it quick response GSH. addition, concentration real sample measured using HRP/GO/ITO-modified electrode.

Язык: Английский

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Glutathione regulates CIA-activated splenic-lymphocytes via NF-κB/MMP-9 and MAPK/PCNA pathways manipulating immune response

Cellular Immunology, Год журнала: 2024, Номер 405-406, С. 104866 - 104866

Опубликована: Сен. 3, 2024

Язык: Английский

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